Restriction map for pF1KB8017
 (Linear) (Six-base) MAPSORT of: KIBB8017.seq  Check: 5703  from: 1  to: 1941



KIBB8017.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     482    1850    1941 
   Size:        482    1368      91

  Fragments arranged by size: 

                1368     482      91
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     132     325    1941 
   Size:        132     193    1616

  Fragments arranged by size: 

                1616     193     132
 
AfeI AGC'GCT

Cuts at:      0    1516    1941 
   Size:       1516     425
 
AflIII A'CryG_T

Cuts at:      0     337    1941 
   Size:        337    1604
 
AhdI GACnn_n'nnGTC

Cuts at:      0     535    1941 
   Size:        535    1406
 
AleI CACnn'nnGTG

Cuts at:      0     376     493    1525    1941 
   Size:        376     117    1032     416

  Fragments arranged by size: 

                1032     416     376     117
 
AlwNI CAG_nnn'CTG

Cuts at:      0     120     450    1648    1941 
   Size:        120     330    1198     293

  Fragments arranged by size: 

                1198     330     293     120
 
ApaI G_GGCC'C

Cuts at:      0     234    1855    1941 
   Size:        234    1621      86

  Fragments arranged by size: 

                1621     234      86
 
ApoI r'AATT_y

Cuts at:      0     642    1941 
   Size:        642    1299
 
AvaI C'yCGr_G

Cuts at:      0     316     760    1728    1771    1941 
   Size:        316     444     968      43     170

  Fragments arranged by size: 

                 968     444     316     170      43
 
BamHI G'GATC_C

Cuts at:      0     612    1941 
   Size:        612    1329
 
BanI G'GyrC_C

Cuts at:      0     574    1548    1941 
   Size:        574     974     393

  Fragments arranged by size: 

                 974     574     393
 
BanII G_rGCy'C

Cuts at:      0     234     631     823     928    1855    1941 
   Size:        234     397     192     105     927      86

  Fragments arranged by size: 

                 927     397     234     192     105      86
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1471    1941 
   Size:       1471     470
 
BclI T'GATC_A

Cuts at:      0    1559    1941 
   Size:       1559     382
 
BglI GCCn_nnn'nGGC

Cuts at:      0     566    1941 
   Size:        566    1375
 
Bme1580I G_kGCm'C

Cuts at:      0     234     407     704    1855    1941 
   Size:        234     173     297    1151      86

  Fragments arranged by size: 

                1151     297     234     173      86
 
BmgBI CAC'GTC

Cuts at:      0     654    1941 
   Size:        654    1287
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     237     793     931    1858    1941 
   Size:        237     556     138     927      83

  Fragments arranged by size: 

                 927     556     237     138      83
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48      97     733    1831    1882    1941 
   Size:         48      49     636    1098      51      59

  Fragments arranged by size: 

                1098     636      59      51      49      48
 
Bpu10I CC'TnA_GC

Cuts at:      0     817    1941 
   Size:        817    1124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     900    1129    1174    1477    1941 
   Size:        900     229      45     303     464

  Fragments arranged by size: 

                 900     464     303     229      45
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     653     799     908    1120    1370    1807    1882    1941 
   Size:        653     146     109     212     250     437      75      59

  Fragments arranged by size: 

                 653     437     250     212     146     109      75      59
            
 
BsaAI yAC'GTr

Cuts at:      0     338    1941 
   Size:        338    1603
 
BsaWI w'CCGG_w

Cuts at:      0     662    1112    1941 
   Size:        662     450     829

  Fragments arranged by size: 

                 829     662     450
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     292     322    1339    1369    1399    1941 
   Size:        292      30    1017      30      30     542

  Fragments arranged by size: 

                1017     542     292      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     897    1138    1941 
   Size:        897     241     803

  Fragments arranged by size: 

                 897     803     241
 
BseYI C'CCAG_C

Cuts at:      0     295     504     795     838     938    1032    1941 
   Size:        295     209     291      43     100      94     909

  Fragments arranged by size: 

                 909     295     291     209     100      94      43
 
BsiEI CG_ry'CG

Cuts at:      0    1006    1569    1941 
   Size:       1006     563     372

  Fragments arranged by size: 

                1006     563     372
 
BsiHKAI G_wGCw'C

Cuts at:      0     262     386     584     823    1190    1208    1941 
   Size:        262     124     198     239     367      18     733

  Fragments arranged by size: 

                 733     367     262     239     198     124      18
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1453    1941 
   Size:       1453     488
 
Bsp1286I G_dGCh'C

Cuts at:      0     234     262     386     407     584     631     704     823 
   Size:        234      28     124      21     177      47      73     119

Cuts at:    823     928    1190    1208    1855    1941 
   Size:        105     262      18     647      86

  Fragments arranged by size: 

                 647     262     234     177     124     119     105      86
                  73      47      28      21      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     482     728    1664    1850    1941 
   Size:        482     246     936     186      91

  Fragments arranged by size: 

                 936     482     246     186      91
 
BsrBI CCG'CTC

Cuts at:      0    1910    1941 
   Size:       1910      31
 
BsrFI r'CCGG_y

Cuts at:      0     983    1834    1895    1941 
   Size:        983     851      61      46

  Fragments arranged by size: 

                 983     851      61      46
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     450    1043    1941 
   Size:        450     593     898

  Fragments arranged by size: 

                 898     593     450
 
BstEII G'GTnAC_C

Cuts at:      0     426    1941 
   Size:        426    1515
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     282    1941 
   Size:        282    1659
 
BstYI r'GATC_y

Cuts at:      0      92     446     612     665    1941 
   Size:         92     354     166      53    1276

  Fragments arranged by size: 

                1276     354     166      92      53
 
Bsu36I CC'TnA_GG

Cuts at:      0    1475    1928    1941 
   Size:       1475     453      13

  Fragments arranged by size: 

                1475     453      13
 
BtgI C'CryG_G

Cuts at:      0     252     789    1020    1297    1523    1667    1941 
   Size:        252     537     231     277     226     144     274

  Fragments arranged by size: 

                 537     277     274     252     231     226     144
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1179    1314    1941 
   Size:       1179     135     627

  Fragments arranged by size: 

                1179     627     135
 
BtsI GCAGTG_nn'

Cuts at:      0     171     405    1941 
   Size:        171     234    1536

  Fragments arranged by size: 

                1536     234     171
 
DraIII CAC_nnn'GTG

Cuts at:      0    1075    1941 
   Size:       1075     866
 
EaeI y'GGCC_r

Cuts at:      0      84     102     985    1003    1050    1566    1832    1836 
   Size:         84      18     883      18      47     516     266       4

Cuts at:   1836    1941 
   Size:        105

  Fragments arranged by size: 

                 883     516     266     105      84      47      18      18
                   4
 
EagI C'GGCC_G

Cuts at:      0    1003    1566    1941 
   Size:       1003     563     375

  Fragments arranged by size: 

                1003     563     375
 
EarI CTCTTCn'nnn_

Cuts at:      0     214    1771    1941 
   Size:        214    1557     170

  Fragments arranged by size: 

                1557     214     170
 
EcoICRI GAG'CTC

Cuts at:      0     821    1941 
   Size:        821    1120
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48      97     132     325     733    1831    1882    1941 
   Size:         48      49      35     193     408    1098      51      59

  Fragments arranged by size: 

                1098     408     193      59      51      49      48      35
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1147    1941 
   Size:       1147     794
 
EcoO109I rG'GnC_Cy

Cuts at:      0     117     344     799     948    1479    1932    1941 
   Size:        117     227     455     149     531     453       9

  Fragments arranged by size: 

                 531     455     453     227     149     117       9
 
EcoRI G'AATT_C

Cuts at:      0     642    1941 
   Size:        642    1299
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1062    1941 
   Size:       1062     879
 
FseI GG_CCGG'CC

Cuts at:      0    1838    1941 
   Size:       1838     103
 
HaeII r_GCGC'y

Cuts at:      0     914    1518    1941 
   Size:        914     604     423

  Fragments arranged by size: 

                 914     604     423
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     521     553     650     682    1318    1350    1369    1401 
   Size:        521      32      97      32     636      32      19      32

Cuts at:   1401    1582    1614    1941 
   Size:        181      32     327

  Fragments arranged by size: 

                 636     521     327     181      97      32      32      32
                  32      32      19
 
HincII GTy'rAC

Cuts at:      0       8    1941 
   Size:          8    1933
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     636     989    1941 
   Size:        636     353     952

  Fragments arranged by size: 

                 952     636     353
 
MscI TGG'CCA

Cuts at:      0     104    1941 
   Size:        104    1837
 
MslI CAynn'nnrTG

Cuts at:      0     376     493    1201    1525    1747    1941 
   Size:        376     117     708     324     222     194

  Fragments arranged by size: 

                 708     376     324     222     194     117
 
MspA1I CmG'CkG

Cuts at:      0     753     842    1860    1869    1941 
   Size:        753      89    1018       9      72

  Fragments arranged by size: 

                1018     753      89      72       9
 
NaeI GCC'GGC

Cuts at:      0     985    1836    1941 
   Size:        985     851     105

  Fragments arranged by size: 

                 985     851     105
 
NcoI C'CATG_G

Cuts at:      0     252    1297    1667    1941 
   Size:        252    1045     370     274

  Fragments arranged by size: 

                1045     370     274     252
 
NgoMIV G'CCGG_C

Cuts at:      0     983    1834    1941 
   Size:        983     851     107

  Fragments arranged by size: 

                 983     851     107
 
NspI r_CATG'y

Cuts at:      0    1827    1941 
   Size:       1827     114
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     423     609     795    1941 
   Size:        423     186     186    1146

  Fragments arranged by size: 

                1146     423     186     186
 
PfoI T'CCnGG_A

Cuts at:      0    1697    1941 
   Size:       1697     244
 
PmlI CAC'GTG

Cuts at:      0     338    1941 
   Size:        338    1603
 
PpuMI rG'GwC_Cy

Cuts at:      0     344     948    1932    1941 
   Size:        344     604     984       9

  Fragments arranged by size: 

                 984     604     344       9
 
PshAI GACnn'nnGTC

Cuts at:      0     433     953    1941 
   Size:        433     520     988

  Fragments arranged by size: 

                 988     520     433
 
PspOMI G'GGCC_C

Cuts at:      0     230    1851    1941 
   Size:        230    1621      90

  Fragments arranged by size: 

                1621     230      90
 
PspXI vC'TCGA_Gb

Cuts at:      0     760    1941 
   Size:        760    1181
 
PstI C_TGCA'G

Cuts at:      0     166     892    1941 
   Size:        166     726    1049

  Fragments arranged by size: 

                1049     726     166
 
PvuII CAG'CTG

Cuts at:      0     753    1860    1869    1941 
   Size:        753    1107       9      72

  Fragments arranged by size: 

                1107     753      72       9
 
SacI G_AGCT'C

Cuts at:      0     823    1941 
   Size:        823    1118
 
SapI GCTCTTCn'nnn_

Cuts at:      0     214    1941 
   Size:        214    1727
 
ScaI AGT'ACT

Cuts at:      0     187    1941 
   Size:        187    1754
 
SfcI C'TryA_G

Cuts at:      0     162     888    1941 
   Size:        162     726    1053

  Fragments arranged by size: 

                1053     726     162
 
SmlI C'TyrA_G

Cuts at:      0     760     879    1144    1189    1492    1941 
   Size:        760     119     265      45     303     449

  Fragments arranged by size: 

                 760     449     303     265     119      45
 
SphI G_CATG'C

Cuts at:      0    1827    1941 
   Size:       1827     114
 
StyI C'CwwG_G

Cuts at:      0     252     275    1297    1322    1667    1941 
   Size:        252      23    1022      25     345     274

  Fragments arranged by size: 

                1022     345     274     252      25      23
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     245    1941 
   Size:        245    1696
 
TatI w'GTAC_w

Cuts at:      0     185     395    1241    1941 
   Size:        185     210     846     700

  Fragments arranged by size: 

                 846     700     210     185
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     578     610    1941 
   Size:        578      32    1331

  Fragments arranged by size: 

                1331     578      32
 
XhoI C'TCGA_G

Cuts at:      0     760    1941 
   Size:        760    1181
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AhdI     AleI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI     BclI     BglI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrFI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI    HaeII    Hin4I
   HincII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NspI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI    PspXI
     PstI    PvuII     SacI     SapI     ScaI     SfcI     SmlI     SphI
     StyI    TaqII     TatI     TstI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI
     BcgI    BciVI    BfrBI    BglII     BlpI     BmtI     BplI    BsaBI
    BsaHI     BsgI    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrGI
   BssHII    BssSI    BstBI  BstZ17I     ClaI    CspCI     DraI     DrdI
     EciI    EcoRV     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PpiI     PsiI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI
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