Restriction map for
pF1KB8005
(Circular) (Six-base) MAPSORT of: pF1KB8005.seq Check: 3499 from: 1 to: 5357
pF1KB8005 5357 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 2395 2395
Size: 5357
Acc65I G'GTAC_C
Cuts at: 2373 2373
Size: 5357
AclI AA'CG_TT
Cuts at: 5197 5197
Size: 5357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 761 1969 2107 3182 3614 4361 4629 499
Size: 262 1208 138 1075 432 747 268 1227
Fragments arranged by size:
1227 1208 1075 747 432 268 262 138
AflIII A'CryG_T
Cuts at: 763 2332 2709 3813 4790 763
Size: 1569 377 1104 977 1330
Fragments arranged by size:
1569 1330 1104 977 377
AgeI A'CCGG_T
Cuts at: 3745 3745
Size: 5357
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1299 2198 1299
Size: 899 4458
AlwNI CAG_nnn'CTG
Cuts at: 262 2662 4229 262
Size: 2400 1567 1390
Fragments arranged by size:
2400 1567 1390
ApaI G_GGCC'C
Cuts at: 140 140
Size: 5357
ApaLI G'TGCA_C
Cuts at: 4127 4127
Size: 5357
ApoI r'AATT_y
Cuts at: 1238 1381 2361 4710 4966 1238
Size: 143 980 2349 256 1629
Fragments arranged by size:
2349 1629 980 256 143
AseI AT'TA_AT
Cuts at: 20 1372 20
Size: 1352 4005
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5357
AvaI C'yCGr_G
Cuts at: 2144 2190 2377 2144
Size: 46 187 5124
Fragments arranged by size:
5124 187 46
BamHI G'GATC_C
Cuts at: 2137 2382 2137
Size: 245 5112
BanI G'GyrC_C
Cuts at: 2373 2787 3035 3070 2373
Size: 414 248 35 4660
Fragments arranged by size:
4660 414 248 35
BanII G_rGCy'C
Cuts at: 140 1979 2371 3401 140
Size: 1839 392 1030 2096
Fragments arranged by size:
2096 1839 1030 392
BbeI G_GCGC'C
Cuts at: 2791 3039 2791
Size: 248 5109
BbsI GAAGACnn'nnnn_
Cuts at: 394 505 767 1957 394
Size: 111 262 1190 3794
Fragments arranged by size:
3794 1190 262 111
BciVI GTATCCnnnnn_n'
Cuts at: 1706 1828 3255 4016 5357 1706
Size: 122 1427 761 1341 1706
Fragments arranged by size:
1706 1427 1341 761 122
BclI T'GATC_A
Cuts at: 2877 2877
Size: 5357
BfrBI ATG'CAT
Cuts at: 1771 1771
Size: 5357
BglII A'GATC_T
Cuts at: 1356 2872 4909 1356
Size: 1516 2037 1804
Fragments arranged by size:
2037 1804 1516
BlpI GC'TnA_GC
Cuts at: 2469 2469
Size: 5357
Bme1580I G_kGCm'C
Cuts at: 140 962 2982 3075 4131 140
Size: 822 2020 93 1056 1366
Fragments arranged by size:
2020 1366 1056 822 93
BmrI ACTGGGnnnn_n'
Cuts at: 2569 2650 2985 5129 2569
Size: 81 335 2144 2797
Fragments arranged by size:
2797 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3724 3724
Size: 5357
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 842 1890 842
Size: 1048 4309
Bpu10I CC'TnA_GC
Cuts at: 218 218
Size: 5357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1738 2533 3412 3904 4202 1738
Size: 795 879 492 298 2893
Fragments arranged by size:
2893 879 795 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 518 1211 1341 5061 518
Size: 693 130 3720 814
Fragments arranged by size:
3720 814 693 130
BsaAI yAC'GTr
Cuts at: 2710 2710
Size: 5357
BsaBI GATnn'nnATC
Cuts at: 6 1688 2871 4908 6
Size: 1682 1183 2037 455
Fragments arranged by size:
2037 1682 1183 455
BsaHI Gr'CG_yC
Cuts at: 2788 3036 3738 5234 2788
Size: 248 702 1496 2911
Fragments arranged by size:
2911 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1536 1686 2546 3067 3745 4019 4166 4577 1536
Size: 150 860 521 678 274 147 411 2316
Fragments arranged by size:
2316 860 678 521 411 274 150 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 533 563 533
Size: 30 5327
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 813 845 813
Size: 32 5325
BseYI C'CCAG_C
Cuts at: 593 2782 3540 4117 4613 593
Size: 2189 758 577 496 1337
Fragments arranged by size:
2189 1337 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2208 2208
Size: 5357
BsiEI CG_ry'CG
Cuts at: 104 679 2899 2945 4153 104
Size: 575 2220 46 1208 1308
Fragments arranged by size:
2220 1308 1208 575 46
BsiHKAI G_wGCw'C
Cuts at: 940 1979 2371 3149 3339 4131 940
Size: 1039 392 778 190 792 2166
Fragments arranged by size:
2166 1039 792 778 392 190
BsmI GAATG_Cn'
Cuts at: 1195 1195
Size: 5357
Bsp1286I G_dGCh'C
Cuts at: 140 940 962 1979 2371 2982 3075 3149 3339
Size: 800 22 1017 392 611 93 74 190
Cuts at: 3339 3401 4131 140
Size: 62 730 1366
Fragments arranged by size:
1366 1017 800 730 611 392 190 93
74 62 22
BspEI T'CCGG_A
Cuts at: 1536 1686 4577 1536
Size: 150 2891 2316
Fragments arranged by size:
2891 2316 150
BspHI T'CATG_A
Cuts at: 1337 4533 1337
Size: 3196 2161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1267 2243 2407 2621 2923 3304 1267
Size: 976 164 214 302 381 3320
Fragments arranged by size:
3320 976 381 302 214 164
BsrBI CCG'CTC
Cuts at: 50 3649 3703 5187 5352 50
Size: 3599 54 1484 165 55
Fragments arranged by size:
3599 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 250 664 1000 1281 1504 1621 2170 3269 250
Size: 414 336 281 223 117 549 1099 2338
Fragments arranged by size:
2338 1099 549 414 336 281 223 117
BsrFI r'CCGG_y
Cuts at: 678 3355 3536 3745 678
Size: 2677 181 209 2290
Fragments arranged by size:
2677 2290 209 181
BsrGI T'GTAC_A
Cuts at: 2044 2044
Size: 5357
BssSI C'ACGA_G
Cuts at: 3628 3986 3628
Size: 358 4999
BstAPI GCAn_nnn'nTGC
Cuts at: 2734 2734
Size: 5357
BstBI TT'CG_AA
Cuts at: 3718 3718
Size: 5357
BstXI CCAn_nnnn'nTGG
Cuts at: 593 1564 593
Size: 971 4386
BstYI r'GATC_y
Cuts at: 1 235 359 780 966 1019 1086 1356 1683
Size: 234 124 421 186 53 67 270 327
Cuts at: 1683 1689 2137 2382 2849 2872 3207 3453 4465
Size: 6 448 245 467 23 335 246 1012
Cuts at: 4465 4551 4563 4909 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 448 421 346 335 327
270 246 245 234 186 124 86 67
53 23 12 6
Bsu36I CC'TnA_GG
Cuts at: 1271 1271
Size: 5357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3307 3488 3651 3307
Size: 181 163 5013
Fragments arranged by size:
5013 181 163
BtsI GCAGTG_nn'
Cuts at: 2600 2600
Size: 5357
DraI TTT'AAA
Cuts at: 1121 2356 1121
Size: 1235 4122
DrdI GACnn_nn'nnGTC
Cuts at: 3063 3921 3063
Size: 858 4499
EaeI y'GGCC_r
Cuts at: 2942 3116 3507 3534 4879 2942
Size: 174 391 27 1345 3420
Fragments arranged by size:
3420 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2942 2942
Size: 5357
EarI CTCTTCn'nnn_
Cuts at: 983 1301 1480 2096 3380 3590 983
Size: 318 179 616 1284 210 2750
Fragments arranged by size:
2750 1284 616 318 210 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3565 3875 4021 5167 3565
Size: 310 146 1146 3755
Fragments arranged by size:
3755 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1977 2369 1977
Size: 392 4965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 761 842 1890 1969 2107 3182 3614 4361
Size: 262 81 1048 79 138 1075 432 747
Cuts at: 4361 4629 499
Size: 268 1227
Fragments arranged by size:
1227 1075 1048 747 432 268 262 138
81 79
EcoNI CCTnn'n_nnAGG
Cuts at: 1274 1274
Size: 5357
EcoO109I rG'GnC_Cy
Cuts at: 2496 2496
Size: 5357
EcoRI G'AATT_C
Cuts at: 2361 2361
Size: 5357
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1417 3376 1417
Size: 1959 3398
FspI TGC'GCA
Cuts at: 3138 3138
Size: 5357
HaeII r_GCGC'y
Cuts at: 669 2791 3039 4061 5040 669
Size: 2122 248 1022 979 986
Fragments arranged by size:
2122 1022 986 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1004 1036 3199 3229 3231 3261 1004
Size: 32 2163 30 2 30 3100
Fragments arranged by size:
3100 2163 32 30 30 2
HincII GTy'rAC
Cuts at: 1056 2396 1056
Size: 1340 4017
KasI G'GCGC_C
Cuts at: 2787 3035 2787
Size: 248 5109
KpnI G_GTAC'C
Cuts at: 2377 2377
Size: 5357
MfeI C'AATT_G
Cuts at: 981 1135 1639 981
Size: 154 504 4699
Fragments arranged by size:
4699 504 154
MluI A'CGCG_T
Cuts at: 4790 4790
Size: 5357
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 222 431 1545 4028 4212 222
Size: 209 1114 2483 184 1367
Fragments arranged by size:
2483 1367 1114 209 184
MscI TGG'CCA
Cuts at: 3118 3118
Size: 5357
MslI CAynn'nnrTG
Cuts at: 1342 3346 3473 1342
Size: 2004 127 3226
Fragments arranged by size:
3226 2004 127
MspA1I CmG'CkG
Cuts at: 2468 2782 3142 4155 4400 2468
Size: 314 360 1013 245 3425
Fragments arranged by size:
3425 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3538 3538
Size: 5357
NarI GG'CG_CC
Cuts at: 2788 3036 2788
Size: 248 5109
NdeI CA'TA_TG
Cuts at: 1773 1773
Size: 5357
NgoMIV G'CCGG_C
Cuts at: 3536 3536
Size: 5357
NsiI A_TGCA'T
Cuts at: 1773 1773
Size: 5357
NspI r_CATG'y
Cuts at: 767 2336 2410 2910 3817 767
Size: 1569 74 500 907 2307
Fragments arranged by size:
2307 1569 907 500 74
PasI CC'CwG_GG
Cuts at: 962 962
Size: 5357
PciI A'CATG_T
Cuts at: 763 2332 3813 763
Size: 1569 1481 2307
Fragments arranged by size:
2307 1569 1481
PflMI CCAn_nnn'nTGG
Cuts at: 745 1427 1468 745
Size: 682 41 4634
Fragments arranged by size:
4634 682 41
PfoI T'CCnGG_A
Cuts at: 1008 1008
Size: 5357
PmeI GTTT'AAAC
Cuts at: 2356 2356
Size: 5357
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1208 1240 4496 4528 1208
Size: 32 3256 32 2037
Fragments arranged by size:
3256 2037 32 32
PshAI GACnn'nnGTC
Cuts at: 560 560
Size: 5357
PsiI TTA'TAA
Cuts at: 1634 1634
Size: 5357
PspOMI G'GGCC_C
Cuts at: 136 136
Size: 5357
PstI C_TGCA'G
Cuts at: 562 1530 1913 2159 2187 2404 3089 562
Size: 968 383 246 28 217 685 2830
Fragments arranged by size:
2830 968 685 383 246 217 28
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5357
PvuII CAG'CTG
Cuts at: 2782 3142 2782
Size: 360 4997
RsrII CG'GwC_CG
Cuts at: 3552 3552
Size: 5357
SacI G_AGCT'C
Cuts at: 1979 2371 1979
Size: 392 4965
SalI G'TCGA_C
Cuts at: 2394 2394
Size: 5357
SapI GCTCTTCn'nnn_
Cuts at: 983 2096 3380 3590 983
Size: 1113 1284 210 2750
Fragments arranged by size:
2750 1284 1113 210
SbfI CC_TGCA'GG
Cuts at: 2404 2404
Size: 5357
ScaI AGT'ACT
Cuts at: 161 4874 161
Size: 4713 644
SexAI A'CCwGG_T
Cuts at: 176 1220 176
Size: 1044 4313
SfcI C'TryA_G
Cuts at: 33 254 558 1526 1909 2155 2183 2400 3085
Size: 221 304 968 383 246 28 217 685
Cuts at: 3085 4078 4269 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 968 685 383 304 246 221
217 191 28
SfoI GGC'GCC
Cuts at: 2789 3037 2789
Size: 248 5109
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5357
SmaI CCC'GGG
Cuts at: 2379 2379
Size: 5357
SmlI C'TyrA_G
Cuts at: 1717 2144 2512 3427 3919 4181 1717
Size: 427 368 915 492 262 2893
Fragments arranged by size:
2893 915 492 427 368 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5357
SphI G_CATG'C
Cuts at: 2410 2910 2410
Size: 500 4857
SspI AAT'ATT
Cuts at: 691 691
Size: 5357
StuI AGG'CCT
Cuts at: 1400 1400
Size: 5357
StyI C'CwwG_G
Cuts at: 1204 1661 2491 2845 1204
Size: 457 830 354 3716
Fragments arranged by size:
3716 830 457 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 542 2940 3073 3532 3741 542
Size: 2398 133 459 209 2158
Fragments arranged by size:
2398 2158 459 209 133
TatI w'GTAC_w
Cuts at: 159 2044 4872 159
Size: 1885 2828 644
Fragments arranged by size:
2828 1885 644
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 536 568 1571 1603 2330 2362 4874 4906 536
Size: 32 1003 32 727 32 2512 32 987
Fragments arranged by size:
2512 1003 987 727 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3154 3154
Size: 5357
XbaI T'CTAG_A
Cuts at: 2388 2388
Size: 5357
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1358 2852 1358
Size: 1494 3863
XhoI C'TCGA_G
Cuts at: 2144 2144
Size: 5357
XmaI C'CCGG_G
Cuts at: 2377 2377
Size: 5357
XmnI GAAnn'nnTTC
Cuts at: 1784 1931 1784
Size: 147 5210
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsiWI
BsmBI BssHII BstEII BstZ17I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NcoI NheI NotI
NruI PacI PmlI PpuMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SwaI ZraI