Restriction map for pF1KB5637
 (Circular) (Six-base) MAPSORT of: pF1KB5637.seq  Check: 4828  from: 1  to: 6344



pF1KB5637  6344 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    918    1320     918 
   Size:        402    5942
 
AatII G_ACGT'C

Cuts at:    315     315 
   Size:       6344
 
AccI GT'mk_AC

Cuts at:   3382    3382 
   Size:       6344
 
Acc65I G'GTAC_C

Cuts at:   1194    3360    1194 
   Size:       2166    4178
 
AclI AA'CG_TT

Cuts at:   6184    6184 
   Size:       6344
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    962    1508    1601    2768    3084    4169    4601    5348    5616 
   Size:        546      93    1167     316    1085     432     747     268

Cuts at:   5616     962 
   Size:       1690

  Fragments arranged by size: 

                1690    1167    1085     747     546     432     316     268
                  93
 
AflIII A'CryG_T

Cuts at:    568    3696    4800    5777     568 
   Size:       3128    1104     977    1135

  Fragments arranged by size: 

                3128    1135    1104     977
 
AgeI A'CCGG_T

Cuts at:   4732    4732 
   Size:       6344
 
AhdI GACnn_n'nnGTC

Cuts at:   2475    2475 
   Size:       6344
 
AleI CACnn'nnGTG

Cuts at:   1090    1090 
   Size:       6344
 
AlwNI CAG_nnn'CTG

Cuts at:    910    1342    3174    3649    5216     910 
   Size:        432    1832     475    1567    2038

  Fragments arranged by size: 

                2038    1832    1567     475     432
 
ApaI G_GGCC'C

Cuts at:    379     379 
   Size:       6344
 
ApaLI G'TGCA_C

Cuts at:   5114    5114 
   Size:       6344
 
ApoI r'AATT_y

Cuts at:   2263    2290    2592    2748    3052    3124    3191    3348    5697 
   Size:         27     302     156     304      72      67     157    2349

Cuts at:   5697    5953    2263 
   Size:        256    2654

  Fragments arranged by size: 

                2654    2349     304     302     256     157     156      72
                  67      27
 
AseI AT'TA_AT

Cuts at:     20    1882    1895    3151      20 
   Size:       1862      13    1256    3213

  Fragments arranged by size: 

                3213    1862    1256      13
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6344
 
AvaI C'yCGr_G

Cuts at:    153    1997    3364     153 
   Size:       1844    1367    3133

  Fragments arranged by size: 

                3133    1844    1367
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1186    1219    1186 
   Size:         33    6311
 
BamHI G'GATC_C

Cuts at:   1521    2845    3369    1521 
   Size:       1324     524    4496

  Fragments arranged by size: 

                4496    1324     524
 
BanI G'GyrC_C

Cuts at:   1194    1808    3360    3774    4022    4057    1194 
   Size:        614    1552     414     248      35    3481

  Fragments arranged by size: 

                3481    1552     614     414     248      35
 
BanII G_rGCy'C

Cuts at:    164     379    1562    1769    3320    3358    4388     164 
   Size:        215    1183     207    1551      38    1030    2120

  Fragments arranged by size: 

                2120    1551    1183    1030     215     207      38
 
BbeI G_GCGC'C

Cuts at:   3778    4026    3778 
   Size:        248    6096
 
BbsI GAAGACnn'nnnn_

Cuts at:   1381    1668    2416    2561    1381 
   Size:        287     748     145    5164

  Fragments arranged by size: 

                5164     748     287     145
 
BbvCI CC'TCA_GC

Cuts at:   1154    1154 
   Size:       6344
 
BciVI GTATCCnnnnn_n'

Cuts at:    353     927    4242    5003    6344     353 
   Size:        574    3315     761    1341     353

  Fragments arranged by size: 

                3315    1341     761     574     353
 
BclI T'GATC_A

Cuts at:   2163    3864    2163 
   Size:       1701    4643
 
BglI GCCn_nnn'nGGC

Cuts at:   1603    1603 
   Size:       6344
 
BglII A'GATC_T

Cuts at:   3859    5896    3859 
   Size:       2037    4307
 
BlpI GC'TnA_GC

Cuts at:   3456    3456 
   Size:       6344
 
Bme1580I G_kGCm'C

Cuts at:    379    3969    4062    5118     379 
   Size:       3590      93    1056    1605

  Fragments arranged by size: 

                3590    1605    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   3556    3637    3972    6116    3556 
   Size:         81     335    2144    3784

  Fragments arranged by size: 

                3784    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4711    4711 
   Size:       6344
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    335     373     473     518    1346    2041    3012     335 
   Size:         38     100      45     828     695     971    3667

  Fragments arranged by size: 

                3667     971     828     695     100      45      38
 
Bpu10I CC'TnA_GC

Cuts at:    163     662     680     698     716     728     734     794     800 
   Size:        499      18      18      18      12       6      60       6

Cuts at:    800     812     830    1154    1320     163 
   Size:         12      18     324     166    5187

  Fragments arranged by size: 

                5187     499     324     166      60      18      18      18
                  18      12      12       6       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    207    3520    4399    4891    5189     207 
   Size:       3313     879     492     298    1362

  Fragments arranged by size: 

                3313    1362     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    387    3112    6048     387 
   Size:       2725    2936     683

  Fragments arranged by size: 

                2936    2725     683
 
BsaAI yAC'GTr

Cuts at:    571    2972    3697     571 
   Size:       2401     725    3218

  Fragments arranged by size: 

                3218    2401     725
 
BsaBI GATnn'nnATC

Cuts at:      6    3858    5895       6 
   Size:       3852    2037     455

  Fragments arranged by size: 

                3852    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    312    3775    4023    4725    6221     312 
   Size:       3463     248     702    1496     435

  Fragments arranged by size: 

                3463    1496     702     435     248
 
BsaWI w'CCGG_w

Cuts at:   3533    4054    4732    5006    5153    5564    3533 
   Size:        521     678     274     147     411    4313

  Fragments arranged by size: 

                4313     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    590     620    1059    1089    1113    1143    2080    2110     590 
   Size:         30     439      30      24      30     937      30    4824

  Fragments arranged by size: 

                4824     937     439      30      30      30      30      24
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    245     275     338     401     422     425     523    1528    1531 
   Size:         30      63      63      21       3      98    1005       3

Cuts at:   1531    1570    1585    2878    3015    3253     245 
   Size:         39      15    1293     137     238    3336

  Fragments arranged by size: 

                3336    1293    1005     238     137      98      63      63
                  39      30      21      15       3       3
 
BseYI C'CCAG_C

Cuts at:    903    1106    2834    3769    4527    5104    5600     903 
   Size:        203    1728     935     758     577     496    1647

  Fragments arranged by size: 

                1728    1647     935     758     577     496     203
 
BsiEI CG_ry'CG

Cuts at:    104     301    3886    3932    5140     104 
   Size:        197    3585      46    1208    1308

  Fragments arranged by size: 

                3585    1308    1208     197      46
 
BsiHKAI G_wGCw'C

Cuts at:    164    2924    3358    4136    4326    5118     164 
   Size:       2760     434     778     190     792    1390

  Fragments arranged by size: 

                2760    1390     792     778     434     190
 
BsmI GAATG_Cn'

Cuts at:    467     467 
   Size:       6344
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1501    1928    3318    1501 
   Size:        427    1390    4527

  Fragments arranged by size: 

                4527    1390     427
 
Bsp1286I G_dGCh'C

Cuts at:    164     379    1562    1769    2924    3320    3358    3969    4062 
   Size:        215    1183     207    1155     396      38     611      93

Cuts at:   4062    4136    4326    4388    5118     164 
   Size:         74     190      62     730    1390

  Fragments arranged by size: 

                1390    1183    1155     730     611     396     215     207
                 190      93      74      62      38
 
BspEI T'CCGG_A

Cuts at:   5564    5564 
   Size:       6344
 
BspHI T'CATG_A

Cuts at:   5520    5520 
   Size:       6344
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    918    1182    1320    3394    3608    3910    4291     918 
   Size:        264     138    2074     214     302     381    2971

  Fragments arranged by size: 

                2971    2074     381     302     264     214     138
 
BsrBI CCG'CTC

Cuts at:     50    1671    4636    4690    6174    6339      50 
   Size:       1621    2965      54    1484     165      55

  Fragments arranged by size: 

                2965    1621    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   4256    4256 
   Size:       6344
 
BsrFI r'CCGG_y

Cuts at:   4342    4523    4732    4342 
   Size:        181     209    5954

  Fragments arranged by size: 

                5954     209     181
 
BssSI C'ACGA_G

Cuts at:   3321    4615    4973    3321 
   Size:       1294     358    4692

  Fragments arranged by size: 

                4692    1294     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1174    3721    1174 
   Size:       2547    3797
 
BstBI TT'CG_AA

Cuts at:   2407    4705    2407 
   Size:       2298    4046
 
BstYI r'GATC_y

Cuts at:      1    1521    2845    3003    3139    3369    3836    3859    4194 
   Size:       1520    1324     158     136     230     467      23     335

Cuts at:   4194    4440    5452    5538    5550    5896       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1520    1324    1012     467     449     346     335     246
                 230     158     136      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     295    1642    1973     107 
   Size:        188    1347     331    4478

  Fragments arranged by size: 

                4478    1347     331     188
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4294    4475    4638    4294 
   Size:        181     163    6000

  Fragments arranged by size: 

                6000     181     163
 
BtsI GCAGTG_nn'

Cuts at:    227    3587     227 
   Size:       3360    2984
 
DraI TTT'AAA

Cuts at:   3343    3343 
   Size:       6344
 
DrdI GACnn_nn'nnGTC

Cuts at:   1827    4050    4908    1827 
   Size:       2223     858    3263

  Fragments arranged by size: 

                3263    2223     858
 
EaeI y'GGCC_r

Cuts at:    298    2375    3929    4103    4494    4521    5866     298 
   Size:       2077    1554     174     391      27    1345     776

  Fragments arranged by size: 

                2077    1554    1345     776     391     174      27
 
EagI C'GGCC_G

Cuts at:    298    3929     298 
   Size:       3631    2713
 
EarI CTCTTCn'nnn_

Cuts at:   1448    1484    1526    1562    1924    2765    4367    4577    1448 
   Size:         36      42      36     362     841    1602     210    3215

  Fragments arranged by size: 

                3215    1602     841     362     210      42      36      36
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    127    4552    4862    5008    6154     127 
   Size:       4425     310     146    1146     317

  Fragments arranged by size: 

                4425    1146     317     310     146
 
EcoICRI GAG'CTC

Cuts at:    162    3356     162 
   Size:       3194    3150
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    335     373     473     518     962    1346    1508    1601    2041 
   Size:         38     100      45     444     384     162      93     440

Cuts at:   2041    2768    3012    3084    4169    4601    5348    5616     335 
   Size:        727     244      72    1085     432     747     268    1063

  Fragments arranged by size: 

                1085    1063     747     727     444     440     432     384
                 268     244     162     100      93      72      45      38
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    350     353     350 
   Size:          3    6341
 
EcoO109I rG'GnC_Cy

Cuts at:    280     376    2813    3483     280 
   Size:         96    2437     670    3141

  Fragments arranged by size: 

                3141    2437     670      96
 
EcoRI G'AATT_C

Cuts at:   3348    3348 
   Size:       6344
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2297    2893    4363    2297 
   Size:        596    1470    4278

  Fragments arranged by size: 

                4278    1470     596
 
FspI TGC'GCA

Cuts at:    986    4125     986 
   Size:       3139    3205
 
HaeII r_GCGC'y

Cuts at:   3778    4026    5048    6027    3778 
   Size:        248    1022     979    4095

  Fragments arranged by size: 

                4095    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    237     269    1113    1145    1939    1971    2461    2493    2527 
   Size:         32     844      32     794      32     490      32      34

Cuts at:   2527    2559    4186    4216    4218    4248     237 
   Size:         32    1627      30       2      30    2333

  Fragments arranged by size: 

                2333    1627     844     794     490      34      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1749    3383    1749 
   Size:       1634    4710
 
HindIII A'AGCT_T

Cuts at:   2875    2875 
   Size:       6344
 
KasI G'GCGC_C

Cuts at:   3774    4022    3774 
   Size:        248    6096
 
KpnI G_GTAC'C

Cuts at:   1198    3364    1198 
   Size:       2166    4178
 
MfeI C'AATT_G

Cuts at:   2330    2330 
   Size:       6344
 
MluI A'CGCG_T

Cuts at:   5777    5777 
   Size:       6344
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    855    1843    5015    5199     855 
   Size:        988    3172     184    2000

  Fragments arranged by size: 

                3172    2000     988     184
 
MscI TGG'CCA

Cuts at:   4105    4105 
   Size:       6344
 
MslI CAynn'nnrTG

Cuts at:   1090    2970    4333    4460    1090 
   Size:       1880    1363     127    2974

  Fragments arranged by size: 

                2974    1880    1363     127
 
MspA1I CmG'CkG

Cuts at:   2380    2834    3455    3769    4129    5142    5387    2380 
   Size:        454     621     314     360    1013     245    3337

  Fragments arranged by size: 

                3337    1013     621     454     360     314     245
 
NaeI GCC'GGC

Cuts at:   4525    4525 
   Size:       6344
 
NarI GG'CG_CC

Cuts at:   3775    4023    3775 
   Size:        248    6096
 
NcoI C'CATG_G

Cuts at:    107    1642    1973     107 
   Size:       1535     331    4478

  Fragments arranged by size: 

                4478    1535     331
 
NdeI CA'TA_TG

Cuts at:   3264    3264 
   Size:       6344
 
NgoMIV G'CCGG_C

Cuts at:   4523    4523 
   Size:       6344
 
NspI r_CATG'y

Cuts at:   1227    2052    2308    3397    3897    4804    1227 
   Size:        825     256    1089     500     907    2767

  Fragments arranged by size: 

                2767    1089     907     825     500     256
 
PciI A'CATG_T

Cuts at:   4800    4800 
   Size:       6344
 
PflMI CCAn_nnn'nTGG

Cuts at:    265     265 
   Size:       6344
 
PfoI T'CCnGG_A

Cuts at:    351     351 
   Size:       6344
 
PmeI GTTT'AAAC

Cuts at:   3343    3343 
   Size:       6344
 
PmlI CAC'GTG

Cuts at:    571    2972     571 
   Size:       2401    3943
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5483    5515    5483 
   Size:         32    6312
 
PpuMI rG'GwC_Cy

Cuts at:   2813    2813 
   Size:       6344
 
PsiI TTA'TAA

Cuts at:    557    1388    1685    3130     557 
   Size:        831     297    1445    3771

  Fragments arranged by size: 

                3771    1445     831     297
 
PspOMI G'GGCC_C

Cuts at:    375     375 
   Size:       6344
 
PstI C_TGCA'G

Cuts at:    915    3391    4076     915 
   Size:       2476     685    3183

  Fragments arranged by size: 

                3183    2476     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6344
 
PvuII CAG'CTG

Cuts at:   3769    4129    3769 
   Size:        360    5984
 
RsrII CG'GwC_CG

Cuts at:   4539    4539 
   Size:       6344
 
SacI G_AGCT'C

Cuts at:    164    3358     164 
   Size:       3194    3150
 
SalI G'TCGA_C

Cuts at:   3381    3381 
   Size:       6344
 
SapI GCTCTTCn'nnn_

Cuts at:   1484    1526    2765    4367    4577    1484 
   Size:         42    1239    1602     210    3251

  Fragments arranged by size: 

                3251    1602    1239     210      42
 
SbfI CC_TGCA'GG

Cuts at:   3391    3391 
   Size:       6344
 
ScaI AGT'ACT

Cuts at:   3221    5861    3221 
   Size:       2640    3704
 
SfcI C'TryA_G

Cuts at:     33     911    1052    1079    3387    4072    5065    5256      33 
   Size:        878     141      27    2308     685     993     191    1121

  Fragments arranged by size: 

                2308    1121     993     878     685     191     141      27
            
 
SfoI GGC'GCC

Cuts at:   3776    4024    3776 
   Size:        248    6096
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6344
 
SmaI CCC'GGG

Cuts at:    155    3366     155 
   Size:       3211    3133
 
SmlI C'TyrA_G

Cuts at:    186    3499    4414    4906    5168     186 
   Size:       3313     915     492     262    1362

  Fragments arranged by size: 

                3313    1362     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6344
 
SphI G_CATG'C

Cuts at:   1227    2308    3397    3897    1227 
   Size:       1081    1089     500    3674

  Fragments arranged by size: 

                3674    1089    1081     500
 
StuI AGG'CCT

Cuts at:    184     184 
   Size:       6344
 
StyI C'CwwG_G

Cuts at:    107    1642    1973    2228    3478    3832     107 
   Size:       1535     331     255    1250     354    2619

  Fragments arranged by size: 

                2619    1535    1250     354     331     255
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1017    1730    1826    2363    3927    4060    4519    4728    1017 
   Size:        713      96     537    1564     133     459     209    2633

  Fragments arranged by size: 

                2633    1564     713     537     459     209     133      96
            
 
TatI w'GTAC_w

Cuts at:   2570    3219    5859    2570 
   Size:        649    2640    3055

  Fragments arranged by size: 

                3055    2640     649
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5861    5893    5861 
   Size:         32    6312
 
Tth111I GACn'n_nGTC

Cuts at:   4141    4141 
   Size:       6344
 
XbaI T'CTAG_A

Cuts at:   3375    3375 
   Size:       6344
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2016    3839    2016 
   Size:       1823    4521
 
XmaI C'CCGG_G

Cuts at:    153    3364     153 
   Size:       3211    3133
 
XmnI GAAnn'nnTTC

Cuts at:   2038    2038 
   Size:       6344
 
ZraI GAC'GTC

Cuts at:    313     313 
   Size:       6344
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AleI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AlfI     AloI     AscI    AvrII     BcgI    BfrBI
    BmgBI     BmtI     BsgI    BsiWI    BsrGI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI
     HpaI     NheI     NotI     NruI     NsiI     PacI     PasI    PshAI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI
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