Restriction map for pF1KB5593
##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AccIII      AclI        AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AspEI       AviII       
AvrII       BbeI        BbrPI       BcgI        BcnI        
BlnI        BsaAI       BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BspDI       BspEI       BsrBI       
BsrFI       BssHII      BstBI       Cfr10I      ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRV       FseI        FspI        
HapII       HindIII     HpaII       KasI        KspI        
MamI        MluI        MroI        MspI        NaeI        
NarI        NciI        NdeI        NgoMIV      NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII         525  GACGTC               GACGT^C
Acc65I       2313  GGTACC               G^GTACC
AccII          19  CGCG                 CG^CG
AcyI          525  GACGTC               G[GA]^CG[CT]C
AflII        1419  CTTAAG               C^TTAAG
AflIII        979  ACATGT               A^C[GA][CT]GT
ApaI         5308  GGGCCC               GGGCC^C
Asp718I      2313  GGTACC               G^GTACC
AspI         5157  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         5501  CCCGAG               C^[CT]CG[GA]G
BalI         1295  TGGCCA               TGG^CCA
BamHI        3939  GGATCC               G^GATCC
BbvCI        1830  GCTGAGG              CCTCAGC(-5/-2)
BclI           89  TGATCA               T^GATCA
BfrI         1419  CTTAAG               C^TTAAG
BglI         1974  GCCAGTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         525  GACGTC               G[GA]^CG[CT]C
BsaI         1518  GAGACC               GGTCTC(1/5)
BsoBI        5501  CCCGAG               C^[CT]CG[GA]G
BspLU11I      979  ACATGT               A^CATGT
BsrDI        4879  GCAATG               GCAATG(2/0)
Bst1107I     2936  GTATAC               GTA^TAC
BstUI          19  CGCG                 CG^CG
BstZ17I      2936  GTATAC               GTA^TAC
DraI         3106  TTTAAA               TTT^AAA
DraIII        108  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         4952  CCATGG               C^C[GA][CT]GG
EaeI         1295  TGGCCA               [CT]^GGCC[GA]
EcoRI         907  GAATTC               G^AATTC
EcoT22I      3422  ATGCAT               ATGCA^T
FauI         5122  GCGGG                CCCGC(4/6)
FbaI           89  TGATCA               T^GATCA
HaeII        2594  AGCGCC               [GA]GCGC^[CT]
Hin1I         525  GACGTC               G[GA]^CG[CT]C
KpnI         2313  GGTACC               GGTAC^C
MluNI        1295  TGGCCA               TGG^CCA
MscI         1295  TGGCCA               TGG^CCA
MvnI           19  CGCG                 CG^CG
NcoI         4952  CCATGG               C^CATGG
NheI         3877  GCTAGC               G^CTAGC
NsiI         3422  ATGCAT               ATGCA^T
PflFI        5157  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         525  GACGTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         4579  GAGCTC               GAGCT^C
ScaI         3148  AGTACT               AGT^ACT
SphI          140  GCATGC               GCATG^C
Tth111I      5157  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4671  TCTAGA               T^CTAGA
XcmI         5327  CCACAGATCAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AocI         1540  CCTGAGG              CC^T[ACGT]AGG
             3271  CCTGAGG             
AspHI        3069  GAGCAC               G[AT]GC[AT]^C
             4579  GAGCTC              
BanI         2313  GGTACC               G^G[CT][GA]CC
             5487  GGCACC              
BlpI          935  GCTTAGC              GC^T[ACGT]AGC
             3555  GCTTAGC             
Bpu1102I      935  GCTTAGC              GC^T[ACGT]AGC
             3555  GCTTAGC             
BsiHKAI      3069  GAGCAC               G[AT]GC[AT]^C
             4579  GAGCTC              
BsmBI        4131  CGTCTC               CGTCTC(1/5)
              688  GAGACG              
Bsp1407I     2757  TGTACA               T^GTACA
             4994  TGTACA              
BspHI        1308  TCATGA               T^CATGA
             1656  TCATGA              
BspMI        2019  ACCTGC               ACCTGC(4/8)
             4322  ACCTGC              
BsrGI        2757  TGTACA               T^GTACA
             4994  TGTACA              
BssSI        4763  CTCGTG               CACGAG(-5/-1)
             4990  CTCGTG              
Bsu36I       1540  CCTGAGG              CC^T[ACGT]AGG
             3271  CCTGAGG             
CelII         935  GCTTAGC              GC^T[ACGT]AGC
             3555  GCTTAGC             
CfoI         1864  GCGC                 GCG^C
             2595  GCGC                
Eco81I       1540  CCTGAGG              CC^T[ACGT]AGG
             3271  CCTGAGG             
EcoNI        1522  CCTACATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3852  CCTTAGTGAGG         
HhaI         1864  GCGC                 GCG^C
             2595  GCGC                
HinP1I       1864  GCGC                 G^CGC
             2595  GCGC                
HpaI         2103  GTTAAC               GTT^AAC
             2509  GTTAAC              
MaeII         526  ACGT                 A^CGT
             1241  ACGT                
PflMI         375  CCAAAGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5541  CCAGGAGCTGG         
PpuMI        2668  GGGTCCT              [GA]G^G[AT]CC[CT]
             4319  AGGACCT             
RcaI         1308  TCATGA               T^CATGA
             1656  TCATGA              
SpeI         1872  ACTAGT               A^CTAGT
             3487  ACTAGT              
SspBI        2757  TGTACA               T^GTACA
             4994  TGTACA              
StuI         1530  AGGCCT               AGG^CCT
             4410  AGGCCT              
TaqI         1721  TCGA                 T^CGA
             1787  TCGA                
TthHB8I      1721  TCGA                 T^CGA
             1787  TCGA                
Van91I        375  CCAAAGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5541  CCAGGAGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
Asp700I     AvaII       BanII       BbsI        BbvI        
BciVI       BfaI        BglII       BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BsrI        BssKI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstXI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoO65I     EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HgaI        HincII      HindII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MfeI        MflI        
MnlI        MseI        MslI        MspA1I      MunI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PshBI       PstI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SexAI       SfaNI       SfcI        SmlI        SspI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XhoII       XmnI        
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