Restriction map for pF1KB5593
 (Linear) (Six-base) MAPSORT of: KIBB5593.seq  Check: 2874  from: 1  to: 5589



KIBB5593.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AatII G_ACGT'C

Cuts at:      0     529    5589 
   Size:        529    5060
 
AccI GT'mk_AC

Cuts at:      0     533    2937    3721    5589 
   Size:        533    2404     784    1868

  Fragments arranged by size: 

                2404    1868     784     533
 
Acc65I G'GTAC_C

Cuts at:      0    2313    5589 
   Size:       2313    3276
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     581    1121    1259    2119    2252    4157    4534    4760 
   Size:        581     540     138     860     133    1905     377     226

Cuts at:   4760    5063    5265    5589 
   Size:        303     202     324

  Fragments arranged by size: 

                1905     860     581     540     377     324     303     226
                 202     138     133
 
AflII C'TTAA_G

Cuts at:      0    1419    5589 
   Size:       1419    4170
 
AflIII A'CryG_T

Cuts at:      0     979    5589 
   Size:        979    4610
 
AleI CACnn'nnGTG

Cuts at:      0    3854    5589 
   Size:       3854    1735
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     603    1877    5453    5589 
   Size:        603    1274    3576     136

  Fragments arranged by size: 

                3576    1274     603     136
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1071    4108    4828    4846    5077    5547    5589 
   Size:       1071    3037     720      18     231     470      42

  Fragments arranged by size: 

                3037    1071     720     470     231      42      18
 
ApaI G_GGCC'C

Cuts at:      0    5312    5589 
   Size:       5312     277
 
ApoI r'AATT_y

Cuts at:      0     133     679     907    2158    3774    4981    5589 
   Size:        133     546     228    1251    1616    1207     608

  Fragments arranged by size: 

                1616    1251    1207     608     546     228     133
 
AseI AT'TA_AT

Cuts at:      0      44    3131    3173    5589 
   Size:         44    3087      42    2416

  Fragments arranged by size: 

                3087    2416      44      42
 
AvaI C'yCGr_G

Cuts at:      0    5501    5589 
   Size:       5501      88
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    5341    5374    5589 
   Size:       5341      33     215

  Fragments arranged by size: 

                5341     215      33
 
BamHI G'GATC_C

Cuts at:      0    3939    5589 
   Size:       3939    1650
 
BanI G'GyrC_C

Cuts at:      0    2313    5487    5589 
   Size:       2313    3174     102

  Fragments arranged by size: 

                3174    2313     102
 
BanII G_rGCy'C

Cuts at:      0    3166    4583    4752    5312    5589 
   Size:       3166    1417     169     560     277

  Fragments arranged by size: 

                3166    1417     560     277     169
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1376    1835    2240    3283    3917    4145    4540    4748 
   Size:       1376     459     405    1043     634     228     395     208

Cuts at:   4748    5589 
   Size:        841

  Fragments arranged by size: 

                1376    1043     841     634     459     405     395     228
                 208
 
BbvCI CC'TCA_GC

Cuts at:      0    1831    5589 
   Size:       1831    3758
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     503    2501    4185    5589 
   Size:        503    1998    1684    1404

  Fragments arranged by size: 

                1998    1684    1404     503
 
BclI T'GATC_A

Cuts at:      0      89    5589 
   Size:         89    5500
 
BfrBI ATG'CAT

Cuts at:      0    3424    5589 
   Size:       3424    2165
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1980    5589 
   Size:       1980    3609
 
BglII A'GATC_T

Cuts at:      0    1020    2244    2284    4668    5276    5589 
   Size:       1020    1224      40    2384     608     313

  Fragments arranged by size: 

                2384    1224    1020     608     313      40
 
BlpI GC'TnA_GC

Cuts at:      0     936    3556    5589 
   Size:        936    2620    2033

  Fragments arranged by size: 

                2620    2033     936
 
Bme1580I G_kGCm'C

Cuts at:      0    5312    5589 
   Size:       5312     277
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     716    5154    5589 
   Size:        716    4438     435

  Fragments arranged by size: 

                4438     716     435
 
BmtI G_CTAG'C

Cuts at:      0    3881    5589 
   Size:       3881    1708
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103    2048    3150    4204    5589 
   Size:        103    1945    1102    1054    1385

  Fragments arranged by size: 

                1945    1385    1102    1054     103
 
Bpu10I CC'TnA_GC

Cuts at:      0    1831    3596    4146    5589 
   Size:       1831    1765     550    1443

  Fragments arranged by size: 

                1831    1765    1443     550
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     611    3105    4336    4556    5589 
   Size:        611    2494    1231     220    1033

  Fragments arranged by size: 

                2494    1231    1033     611     220
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1512    5589 
   Size:       1512    4077
 
BsaHI Gr'CG_yC

Cuts at:      0     526    5589 
   Size:        526    5063
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1442    1472    2252    2282    5204    5234    5589 
   Size:       1442      30     780      30    2922      30     355

  Fragments arranged by size: 

                2922    1442     780     355      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1848    3784    4591    4618    5225    5589 
   Size:       1848    1936     807      27     607     364

  Fragments arranged by size: 

                1936    1848     807     607     364      27
 
BseYI C'CCAG_C

Cuts at:      0    1000    4867    5589 
   Size:       1000    3867     722

  Fragments arranged by size: 

                3867    1000     722
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     776    2435    3918    5449    5589 
   Size:        776    1659    1483    1531     140

  Fragments arranged by size: 

                1659    1531    1483     776     140
 
BsiHKAI G_wGCw'C

Cuts at:      0    3073    4583    5589 
   Size:       3073    1510    1006

  Fragments arranged by size: 

                3073    1510    1006
 
BsmI GAATG_Cn'

Cuts at:      0    1032    1133    3246    5589 
   Size:       1032     101    2113    2343

  Fragments arranged by size: 

                2343    2113    1032     101
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     682    4137    5589 
   Size:        682    3455    1452

  Fragments arranged by size: 

                3455    1452     682
 
Bsp1286I G_dGCh'C

Cuts at:      0    3073    3166    4583    4752    5312    5589 
   Size:       3073      93    1417     169     560     277

  Fragments arranged by size: 

                3073    1417     560     277     169      93
 
BspHI T'CATG_A

Cuts at:      0    1308    1656    5589 
   Size:       1308     348    3933

  Fragments arranged by size: 

                3933    1308     348
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2028    4331    5589 
   Size:       2028    2303    1258

  Fragments arranged by size: 

                2303    2028    1258
 
BsrDI GCAATG_nn'

Cuts at:      0    4886    5589 
   Size:       4886     703
 
BsrGI T'GTAC_A

Cuts at:      0    2757    4994    5589 
   Size:       2757    2237     595

  Fragments arranged by size: 

                2757    2237     595
 
BssSI C'ACGA_G

Cuts at:      0    4763    4990    5589 
   Size:       4763     227     599

  Fragments arranged by size: 

                4763     599     227
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     137    1071    4828    5589 
   Size:        137     934    3757     761

  Fragments arranged by size: 

                3757     934     761     137
 
BstEII G'GTnAC_C

Cuts at:      0    3601    5154    5496    5589 
   Size:       3601    1553     342      93

  Fragments arranged by size: 

                3601    1553     342      93
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      81    3997    5441    5470    5589 
   Size:         81    3916    1444      29     119

  Fragments arranged by size: 

                3916    1444     119      81      29
 
BstYI r'GATC_y

Cuts at:      0     547    1020    2244    2284    3939    4668    5276    5570 
   Size:        547     473    1224      40    1655     729     608     294

Cuts at:   5570    5589 
   Size:         19

  Fragments arranged by size: 

                1655    1224     729     608     547     473     294      40
                  19
 
BstZ17I GTA'TAC

Cuts at:      0    2938    5589 
   Size:       2938    2651
 
Bsu36I CC'TnA_GG

Cuts at:      0    1541    3272    5589 
   Size:       1541    1731    2317

  Fragments arranged by size: 

                2317    1731    1541
 
BtgI C'CryG_G

Cuts at:      0    4952    5589 
   Size:       4952     637
 
BtsI GCAGTG_nn'

Cuts at:      0     597    1944    2774    3906    5589 
   Size:        597    1347     830    1132    1683

  Fragments arranged by size: 

                1683    1347    1132     830     597
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     109     144    4619    4654    5589 
   Size:        109      35    4475      35     935

  Fragments arranged by size: 

                4475     935     109      35      35
 
DraI TTT'AAA

Cuts at:      0    3108    5589 
   Size:       3108    2481
 
DraIII CAC_nnn'GTG

Cuts at:      0     113    5589 
   Size:        113    5476
 
EaeI y'GGCC_r

Cuts at:      0    1295    5589 
   Size:       1295    4294
 
EarI CTCTTCn'nnn_

Cuts at:      0      20     245    1937    2032    2189    2630    3603    3632 
   Size:         20     225    1692      95     157     441     973      29

Cuts at:   3632    3656    4064    4311    4613    4714    5039    5589 
   Size:         24     408     247     302     101     325     550

  Fragments arranged by size: 

                1692     973     550     441     408     325     302     247
                 225     157     101      95      29      24      20
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1008    5589 
   Size:       1008    4581
 
EcoICRI GAG'CTC

Cuts at:      0    4581    5589 
   Size:       4581    1008
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     581    1121    1259    2048    2119    2252    3150 
   Size:        103     478     540     138     789      71     133     898

Cuts at:   3150    4157    4204    4534    4760    5063    5265    5589 
   Size:       1007      47     330     226     303     202     324

  Fragments arranged by size: 

                1007     898     789     540     478     330     324     303
                 226     202     138     133     103      71      47
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1526    3856    5589 
   Size:       1526    2330    1733

  Fragments arranged by size: 

                2330    1733    1526
 
EcoO109I rG'GnC_Cy

Cuts at:      0     169    2669    4320    5309    5589 
   Size:        169    2500    1651     989     280

  Fragments arranged by size: 

                2500    1651     989     280     169
 
EcoRI G'AATT_C

Cuts at:      0     907    5589 
   Size:        907    4682
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3571    3813    4322    5589 
   Size:       3571     242     509    1267

  Fragments arranged by size: 

                3571    1267     509     242
 
HaeII r_GCGC'y

Cuts at:      0    2598    5589 
   Size:       2598    2991
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     222     254     501     533    1189    1195    1221    1227 
   Size:        222      32     247      32     656       6      26       6

Cuts at:   1227    3631    3663    3739    3771    4711    4743    5204    5236 
   Size:       2404      32      76      32     940      32     461      32

Cuts at:   5236    5371    5403    5589 
   Size:        135      32     186

  Fragments arranged by size: 

                2404     940     656     461     247     222     186     135
                  76      32      32      32      32      32      32      32
                  26       6       6
 
HincII GTy'rAC

Cuts at:      0     241    2105    2511    4938    5589 
   Size:        241    1864     406    2427     651

  Fragments arranged by size: 

                2427    1864     651     406     241
 
HpaI GTT'AAC

Cuts at:      0    2105    2511    5589 
   Size:       2105     406    3078

  Fragments arranged by size: 

                3078    2105     406
 
KpnI G_GTAC'C

Cuts at:      0    2317    5589 
   Size:       2317    3272
 
MfeI C'AATT_G

Cuts at:      0    4810    5417    5468    5589 
   Size:       4810     607      51     121

  Fragments arranged by size: 

                4810     607     121      51
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     486    3702    5495    5589 
   Size:        486    3216    1793      94

  Fragments arranged by size: 

                3216    1793     486      94
 
MscI TGG'CCA

Cuts at:      0    1297    5589 
   Size:       1297    4292
 
MslI CAynn'nnrTG

Cuts at:      0    1859    2536    3854    4110    4957    5320    5589 
   Size:       1859     677    1318     256     847     363     269

  Fragments arranged by size: 

                1859    1318     847     677     363     269     256
 
MspA1I CmG'CkG

Cuts at:      0     507     760    4614    4843    5355    5589 
   Size:        507     253    3854     229     512     234

  Fragments arranged by size: 

                3854     512     507     253     234     229
 
NcoI C'CATG_G

Cuts at:      0    4952    5589 
   Size:       4952     637
 
NheI G'CTAG_C

Cuts at:      0    3877    5589 
   Size:       3877    1712
 
NsiI A_TGCA'T

Cuts at:      0    3426    5589 
   Size:       3426    2163
 
NspI r_CATG'y

Cuts at:      0     144     811     983    4530    5325    5589 
   Size:        144     667     172    3547     795     264

  Fragments arranged by size: 

                3547     795     667     264     172     144
 
PciI A'CATG_T

Cuts at:      0     979    5589 
   Size:        979    4610
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     381    5547    5589 
   Size:        381    5166      42

  Fragments arranged by size: 

                5166     381      42
 
PfoI T'CCnGG_A

Cuts at:      0    2622    3294    5589 
   Size:       2622     672    2295

  Fragments arranged by size: 

                2622    2295     672
 
PpuMI rG'GwC_Cy

Cuts at:      0    2669    4320    5589 
   Size:       2669    1651    1269

  Fragments arranged by size: 

                2669    1651    1269
 
PshAI GACnn'nnGTC

Cuts at:      0     529    5589 
   Size:        529    5060
 
PspOMI G'GGCC_C

Cuts at:      0    5308    5589 
   Size:       5308     281
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1226    1258    5589 
   Size:       1226      32    4331

  Fragments arranged by size: 

                4331    1226      32
 
PstI C_TGCA'G

Cuts at:      0    2781    3811    4443    5589 
   Size:       2781    1030     632    1146

  Fragments arranged by size: 

                2781    1146    1030     632
 
PvuII CAG'CTG

Cuts at:      0     760    4614    4843    5355    5589 
   Size:        760    3854     229     512     234

  Fragments arranged by size: 

                3854     760     512     234     229
 
SacI G_AGCT'C

Cuts at:      0    4583    5589 
   Size:       4583    1006
 
SapI GCTCTTCn'nnn_

Cuts at:      0      20     245    3656    4064    5589 
   Size:         20     225    3411     408    1525

  Fragments arranged by size: 

                3411    1525     408     225      20
 
ScaI AGT'ACT

Cuts at:      0    3150    5589 
   Size:       3150    2439
 
SexAI A'CCwGG_T

Cuts at:      0    2115    2316    3815    5589 
   Size:       2115     201    1499    1774

  Fragments arranged by size: 

                2115    1774    1499     201
 
SfcI C'TryA_G

Cuts at:      0     387     412    2288    2777    3807    4439    5589 
   Size:        387      25    1876     489    1030     632    1150

  Fragments arranged by size: 

                1876    1150    1030     632     489     387      25
 
SmlI C'TyrA_G

Cuts at:      0     626    1419    3120    4315    4571    5589 
   Size:        626     793    1701    1195     256    1018

  Fragments arranged by size: 

                1701    1195    1018     793     626     256
 
SpeI A'CTAG_T

Cuts at:      0    1872    3487    5589 
   Size:       1872    1615    2102

  Fragments arranged by size: 

                2102    1872    1615
 
SphI G_CATG'C

Cuts at:      0     144    5589 
   Size:        144    5445
 
SspI AAT'ATT

Cuts at:      0    1264    1365    1379    1641    3129    3824    5107    5589 
   Size:       1264     101      14     262    1488     695    1283     482

  Fragments arranged by size: 

                1488    1283    1264     695     482     262     101      14
            
 
StuI AGG'CCT

Cuts at:      0    1532    4412    5589 
   Size:       1532    2880    1177

  Fragments arranged by size: 

                2880    1532    1177
 
StyI C'CwwG_G

Cuts at:      0     474    1111    2432    4660    4952    5238    5397    5589 
   Size:        474     637    1321    2228     292     286     159     192

  Fragments arranged by size: 

                2228    1321     637     474     292     286     192     159
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3595    3949    4100    5142    5429    5499    5589 
   Size:       3595     354     151    1042     287      70      90

  Fragments arranged by size: 

                3595    1042     354     287     151      90      70
 
TatI w'GTAC_w

Cuts at:      0      30    1906    1961    2757    3061    3148    4994    5589 
   Size:         30    1876      55     796     304      87    1846     595

  Fragments arranged by size: 

                1876    1846     796     595     304      87      55      30
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2653    2685    3924    3956    5589 
   Size:       2653      32    1239      32    1633

  Fragments arranged by size: 

                2653    1633    1239      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    5160    5589 
   Size:       5160     429
 
XbaI T'CTAG_A

Cuts at:      0    4671    5589 
   Size:       4671     918
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    5334    5589 
   Size:       5334     255
 
XmnI GAAnn'nnTTC

Cuts at:      0    1106    1337    2578    3200    3347    3665    5589 
   Size:       1106     231    1241     622     147     318    1924

  Fragments arranged by size: 

                1924    1241    1106     622     318     231     147
 
ZraI GAC'GTC

Cuts at:      0     527    5589 
   Size:        527    5062
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI    AflII   AflIII     AleI     AlfI
    AlwNI     ApaI     ApoI     AseI     AvaI     BaeI    BamHI     BanI
    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI     DraI
   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII     HpaI     KpnI     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI     NheI     NsiI     NspI
     PciI    PflMI     PfoI    PpuMI    PshAI   PspOMI     PsrI     PstI
    PvuII     SacI     SapI     ScaI    SexAI     SfcI     SmlI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI     AgeI     AhdI     AloI    ApaLI     AscI
    AsiSI    AvrII     BbeI     BcgI    BmgBI     BplI    BsaAI    BsaBI
    BsaWI    BsiEI    BsiWI    BspEI    BsrBI    BsrFI   BssHII    BstBI
    BtgZI     ClaI     DrdI     EagI    EcoRV     FseI     FspI    FspAI
  HindIII     KasI     MluI     NaeI     NarI     NdeI   NgoMIV     NotI
     NruI     PacI     PasI     PmeI     PmlI     PpiI     PsiI    PspXI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SrfI     SwaI     XhoI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com