Restriction map for pF1KB5414
 (Linear) (Six-base) MAPSORT of: KIBB5414.seq  Check: 9092  from: 1  to: 1023



KIBB5414.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     495    1023 
   Size:        495     528
 
Acc65I G'GTAC_C

Cuts at:      0     589    1023 
   Size:        589     434
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464     488     857    1023 
   Size:        464      24     369     166

  Fragments arranged by size: 

                 464     369     166      24
 
AflII C'TTAA_G

Cuts at:      0     994    1023 
   Size:        994      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0      58     487     609    1023 
   Size:         58     429     122     414

  Fragments arranged by size: 

                 429     414     122      58
 
ApoI r'AATT_y

Cuts at:      0     377     407    1023 
   Size:        377      30     616

  Fragments arranged by size: 

                 616     377      30
 
AvaI C'yCGr_G

Cuts at:      0     890    1023 
   Size:        890     133
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     573     606    1023 
   Size:        573      33     417

  Fragments arranged by size: 

                 573     417      33
 
BanI G'GyrC_C

Cuts at:      0     507     589    1023 
   Size:        507      82     434

  Fragments arranged by size: 

                 507     434      82
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     254    1023 
   Size:        254     769
 
BglI GCCn_nnn'nGGC

Cuts at:      0     180    1023 
   Size:        180     843
 
BglII A'GATC_T

Cuts at:      0     695    1023 
   Size:        695     328
 
Bme1580I G_kGCm'C

Cuts at:      0     707    1023 
   Size:        707     316
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     758     812    1023 
   Size:         35     723      54     211

  Fragments arranged by size: 

                 723     211      54      35
 
BsaWI w'CCGG_w

Cuts at:      0     292    1023 
   Size:        292     731
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     487     578     994    1023 
   Size:        487      91     416      29

  Fragments arranged by size: 

                 487     416      91      29
 
BseYI C'CCAG_C

Cuts at:      0      86     218     706     813    1023 
   Size:         86     132     488     107     210

  Fragments arranged by size: 

                 488     210     132     107      86
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     734    1023 
   Size:        734     289
 
Bsp1286I G_dGCh'C

Cuts at:      0     707    1023 
   Size:        707     316
 
BspEI T'CCGG_A

Cuts at:      0     292    1023 
   Size:        292     731
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     495    1023 
   Size:        495     528
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     133    1023 
   Size:        133     890
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      34    1023 
   Size:         34     989
 
BstYI r'GATC_y

Cuts at:      0     695    1023 
   Size:        695     328
 
BtgI C'CryG_G

Cuts at:      0       5     198    1023 
   Size:          5     193     825

  Fragments arranged by size: 

                 825     193       5
 
BtsI GCAGTG_nn'

Cuts at:      0     540     953    1023 
   Size:        540     413      70

  Fragments arranged by size: 

                 540     413      70
 
EarI CTCTTCn'nnn_

Cuts at:      0     452     464    1023 
   Size:        452      12     559

  Fragments arranged by size: 

                 559     452      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     464     488     758     812     857    1023 
   Size:         35     429      24     270      54      45     166

  Fragments arranged by size: 

                 429     270     166      54      45      35      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     606     999    1023 
   Size:        606     393      24

  Fragments arranged by size: 

                 606     393      24
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     410    1023 
   Size:        410     613
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     680     712    1023 
   Size:        680      32     311

  Fragments arranged by size: 

                 680     311      32
 
KpnI G_GTAC'C

Cuts at:      0     593    1023 
   Size:        593     430
 
MspA1I CmG'CkG

Cuts at:      0      46     171     576    1023 
   Size:         46     125     405     447

  Fragments arranged by size: 

                 447     405     125      46
 
NcoI C'CATG_G

Cuts at:      0       5     198    1023 
   Size:          5     193     825

  Fragments arranged by size: 

                 825     193       5
 
PasI CC'CwG_GG

Cuts at:      0     558    1023 
   Size:        558     465
 
PpuMI rG'GwC_Cy

Cuts at:      0     999    1023 
   Size:        999      24
 
PvuII CAG'CTG

Cuts at:      0      46     171     576    1023 
   Size:         46     125     405     447

  Fragments arranged by size: 

                 447     405     125      46
 
SfcI C'TryA_G

Cuts at:      0     399     664    1023 
   Size:        399     265     359

  Fragments arranged by size: 

                 399     359     265
 
SmlI C'TyrA_G

Cuts at:      0     890     994    1023 
   Size:        890     104      29

  Fragments arranged by size: 

                 890     104      29
 
StuI AGG'CCT

Cuts at:      0     393    1023 
   Size:        393     630
 
StyI C'CwwG_G

Cuts at:      0       5     198     445    1023 
   Size:          5     193     247     578

  Fragments arranged by size: 

                 578     247     193       5
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     786    1023 
   Size:        786     237
 
TatI w'GTAC_w

Cuts at:      0     165    1023 
   Size:        165     858
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     532    1023 
   Size:        532     491
 
XhoI C'TCGA_G

Cuts at:      0     890    1023 
   Size:        890     133
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AflII    AlwNI     ApoI     AvaI     BaeI
     BanI     BbsI     BglI    BglII Bme1580I     BpmI    BsaWI    BseRI
    BseYI    BsmBI Bsp1286I    BspEI    BspMI   BstAPI    BstXI    BstYI
     BtgI     BtsI     EarI  Eco57MI EcoO109I     FalI    Hin4I     KpnI
   MspA1I     NcoI     PasI    PpuMI    PvuII     SfcI     SmlI     StuI
     StyI    TaqII     TatI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI  Tth111I     XbaI     XmaI     XmnI     ZraI
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