Restriction map for pF1KB4393
##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        AspEI       
AspI        AviII       BbeI        BfrI        BlpI        
Bpu1102I    BsaHI       BsiWI       BspLU11I    BsrBI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     CelII       CpoI        Eam1105I    Eco47III    
EcoO65I     EcoRV       EcoT22I     FspI        Hin1I       
HpaI        KasI        KspI        MluI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SwaI        Tth111I     XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I        490  GGTACC               G^GTACC
AccII        3282  CGCG                 CG^CG
AccIII       1124  TCCGGA               T^CCGGA
Asp718I       490  GGTACC               G^GTACC
AvrII        1962  CCTAGG               C^CTAGG
BbrPI        3856  CACGTG               CAC^GTG
BbvCI        2230  GCTGAGG              CCTCAGC(-5/-2)
BcgI         3836  GCATGAGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2605  GGATAC               GTATCC(6/5)
BclI          914  TGATCA               T^GATCA
BglI         2050  GCCAGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1962  CCTAGG               C^CTAGG
BsaBI        1825  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1124  TCCGGA               T^CCGGA
BsiEI        2559  CGGCCG               CG[GA][CT]^CG
BsmBI          22  GAGACG               CGTCTC(1/5)
BspDI        1363  ATCGAT               AT^CGAT
BspEI        1124  TCCGGA               T^CCGGA
BsrDI        1701  CATTGC               GCAATG(2/0)
BssSI        3298  CTCGTG               CACGAG(-5/-1)
BstUI        3282  CGCG                 CG^CG
ClaI         1363  ATCGAT               AT^CGAT
DrdI         2841  GACCACAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2559  CGGCCG               C^GGCCG
EclXI        2559  CGGCCG               C^GGCCG
Eco52I       2559  CGGCCG               C^GGCCG
EcoRI        4147  GAATTC               G^AATTC
FauI         3696  GCGGG                CCCGC(4/6)
FbaI          914  TGATCA               T^GATCA
FseI         2556  GGCCGGCC             GGCCGG^CC
HaeII         478  GGCGCT               [GA]GCGC^[CT]
HincII       2575  GTCGAC               GT[CT]^[GA]AC
HindII       2575  GTCGAC               GT[CT]^[GA]AC
HindIII      3670  AAGCTT               A^AGCTT
KpnI          490  GGTACC               GGTAC^C
MamI         1825  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2090  CAATTG               C^AATTG
MroI         1124  TCCGGA               T^CCGGA
MunI         2090  CAATTG               C^AATTG
MvnI         3282  CGCG                 CG^CG
PaeR7I       2420  CTCGAG               C^TCGAG
PmaCI        3856  CACGTG               CAC^GTG
PmlI         3856  CACGTG               CAC^GTG
SalI         2575  GTCGAC               G^TCGAC
SapI         4074  GCTCTTC              GCTCTTC(1/4)
SbfI         2895  CCTGCAGG             CCTGCA^GG
ScaI         1868  AGTACT               AGT^ACT
Sse8387I     2895  CCTGCAGG             CCTGCA^GG
SspI         1984  AATATT               AAT^ATT
StuI         1816  AGGCCT               AGG^CCT
XhoI         2420  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI         2065  GTCTAC               GT^[AC][GT]AC
             2575  GTCGAC              
BamHI         558  GGATCC               G^GATCC
             1972  GGATCC              
BsaAI        3559  TACGTG               [CT]AC^GT[GA]
             3856  CACGTG              
Bsp1407I      122  TGTACA               T^GTACA
             1477  TGTACA              
BsrFI        2155  GCCGGC               [GA]^CCGG[CT]
             2557  GCCGGC              
BsrGI         122  TGTACA               T^GTACA
             1477  TGTACA              
Cfr10I       2155  GCCGGC               [GA]^CCGG[CT]
             2557  GCCGGC              
DraI          679  TTTAAA               TTT^AAA
             2761  TTTAAA              
EarI         4075  CTCTTC               CTCTTC(1/4)
             2862  GAAGAG              
EcoNI        2113  CCTGGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3573  CCTGAAAGAGG         
HgaI         3280  GACGC                GACGC(5/10)
             3988  GACGC               
Ksp632I      4075  CTCTTC               CTCTTC(1/4)
             2862  GAAGAG              
MaeII        3560  ACGT                 A^CGT
             3857  ACGT                
NaeI         2155  GCCGGC               GCC^GGC
             2557  GCCGGC              
NcoI         2336  CCATGG               C^CATGG
             3821  CCATGG              
NgoMIV       2155  GCCGGC               G^CCGGC
             2557  GCCGGC              
NspI          141  ACATGC               [GA]CATG^[CT]
             2609  ACATGC              
PleI         4012  GACTC                GAGTC(4/5)
             2848  GAGTC               
PpuMI        2446  AGGTCCT              [GA]G^G[AT]CC[CT]
             4103  GGGACCC             
SspBI         122  TGTACA               T^GTACA
             1477  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspHI       BspMI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
Eco81I      EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PstI        PvuII       
RcaI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmnI        
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