Restriction map for pF1KB4393
 (Linear) (Six-base) MAPSORT of: KIBB4393.seq  Check: 7469  from: 1  to: 4170



KIBB4393.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3157    4170 
   Size:       3157    1013
 
AccI GT'mk_AC

Cuts at:      0    2066    2576    4170 
   Size:       2066     510    1594

  Fragments arranged by size: 

                2066    1594     510
 
Acc65I G'GTAC_C

Cuts at:      0     490    4170 
   Size:        490    3680
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     276     394     565     716    1054    1440    1785    2149 
   Size:        276     118     171     151     338     386     345     364

Cuts at:   2149    2278    3169    4170 
   Size:        129     891    1001

  Fragments arranged by size: 

                1001     891     386     364     345     338     276     171
                 151     129     118
 
AleI CACnn'nnGTG

Cuts at:      0    3823    4170 
   Size:       3823     347
 
AlwNI CAG_nnn'CTG

Cuts at:      0     375    2297    3091    3573    4170 
   Size:        375    1922     794     482     597

  Fragments arranged by size: 

                1922     794     597     482     375
 
ApoI r'AATT_y

Cuts at:      0     573     672    1163    2911    4147    4170 
   Size:        573      99     491    1748    1236      23

  Fragments arranged by size: 

                1748    1236     573     491      99      23
 
AvaI C'yCGr_G

Cuts at:      0     975    1593    2420    4170 
   Size:        975     618     827    1750

  Fragments arranged by size: 

                1750     975     827     618
 
AvrII C'CTAG_G

Cuts at:      0    1962    4170 
   Size:       1962    2208
 
BamHI G'GATC_C

Cuts at:      0     558    1972    4170 
   Size:        558    1414    2198

  Fragments arranged by size: 

                2198    1414     558
 
BanI G'GyrC_C

Cuts at:      0     490     768     817     996    1642    3133    3727    4170 
   Size:        490     278      49     179     646    1491     594     443

  Fragments arranged by size: 

                1491     646     594     490     443     278     179      49
            
 
BanII G_rGCy'C

Cuts at:      0     974    1331    2990    3550    4076    4170 
   Size:        974     357    1659     560     526      94

  Fragments arranged by size: 

                1659     974     560     526     357      94
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2784    3157    3973    4170 
   Size:       2784     373     816     197

  Fragments arranged by size: 

                2784     816     373     197
 
BbvCI CC'TCA_GC

Cuts at:      0    2231    4170 
   Size:       2231    1939
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3825    3859    4170 
   Size:       3825      34     311

  Fragments arranged by size: 

                3825     311      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2599    4170 
   Size:       2599    1571
 
BclI T'GATC_A

Cuts at:      0     914    4170 
   Size:        914    3256
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2056    4170 
   Size:       2056    2114
 
BglII A'GATC_T

Cuts at:      0    1796    1887    2640    4170 
   Size:       1796      91     753    1530

  Fragments arranged by size: 

                1796    1530     753      91
 
Bme1580I G_kGCm'C

Cuts at:      0     642     954    3075    3114    3203    3730    4170 
   Size:        642     312    2121      39      89     527     440

  Fragments arranged by size: 

                2121     642     527     440     312      89      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     936    2019    2646    2798    4170 
   Size:        936    1083     627     152    1372

  Fragments arranged by size: 

                1372    1083     936     627     152
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1081    4170 
   Size:       1081    3089
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283    2263    3064    4170 
   Size:        283    1980     801    1106

  Fragments arranged by size: 

                1980    1106     801     283
 
Bpu10I CC'TnA_GC

Cuts at:      0    2231    4170 
   Size:       2231    1939
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1230    1307    1315    1645    4170 
   Size:       1230      77       8     330    2525

  Fragments arranged by size: 

                2525    1230     330      77       8
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2230    2931    3040    4170 
   Size:       2230     701     109    1130

  Fragments arranged by size: 

                2230    1130     701     109
 
BsaAI yAC'GTr

Cuts at:      0    3561    3858    4170 
   Size:       3561     297     312

  Fragments arranged by size: 

                3561     312     297
 
BsaBI GATnn'nnATC

Cuts at:      0    1829    4170 
   Size:       1829    2341
 
BsaWI w'CCGG_w

Cuts at:      0    1124    1448    2518    4170 
   Size:       1124     324    1070    1652

  Fragments arranged by size: 

                1652    1124    1070     324
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     256     286    1361    1391    4170 
   Size:        256      30    1075      30    2779

  Fragments arranged by size: 

                2779    1075     256      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91     373    1642    2248    2723    4170 
   Size:         91     282    1269     606     475    1447

  Fragments arranged by size: 

                1447    1269     606     475     282      91
 
BseYI C'CCAG_C

Cuts at:      0     630     690    2481    4170 
   Size:        630      60    1791    1689

  Fragments arranged by size: 

                1791    1689     630      60
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1387    2208    3444    3622    4170 
   Size:       1387     821    1236     178     548

  Fragments arranged by size: 

                1387    1236     821     548     178
 
BsiEI CG_ry'CG

Cuts at:      0    2562    4170 
   Size:       2562    1608
 
BsiHKAI G_wGCw'C

Cuts at:      0     167     974    1331    3105    3550    4170 
   Size:        167     807     357    1774     445     620

  Fragments arranged by size: 

                1774     807     620     445     357     167
 
BsmI GAATG_Cn'

Cuts at:      0     980    2157    3608    4170 
   Size:        980    1177    1451     562

  Fragments arranged by size: 

                1451    1177     980     562
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      16    4170 
   Size:         16    4154
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     642     954     974    1331    2990    3075    3105 
   Size:        167     475     312      20     357    1659      85      30

Cuts at:   3105    3114    3203    3550    3730    4076    4170 
   Size:          9      89     347     180     346      94

  Fragments arranged by size: 

                1659     475     357     347     346     312     180     167
                  94      89      85      30      20       9
 
BspEI T'CCGG_A

Cuts at:      0    1124    4170 
   Size:       1124    3046
 
BspHI T'CATG_A

Cuts at:      0     525    2855    3878    4170 
   Size:        525    2330    1023     292

  Fragments arranged by size: 

                2330    1023     525     292
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     340    1856    3157    3701    4170 
   Size:        340    1516    1301     544     469

  Fragments arranged by size: 

                1516    1301     544     469     340
 
BsrDI GCAATG_nn'

Cuts at:      0    1700    4170 
   Size:       1700    2470
 
BsrFI r'CCGG_y

Cuts at:      0    2155    2557    4170 
   Size:       2155     402    1613

  Fragments arranged by size: 

                2155    1613     402
 
BsrGI T'GTAC_A

Cuts at:      0     122    1477    4170 
   Size:        122    1355    2693

  Fragments arranged by size: 

                2693    1355     122
 
BssSI C'ACGA_G

Cuts at:      0    3298    4170 
   Size:       3298     872
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     332    2297    3100    3861    4170 
   Size:        332    1965     803     761     309

  Fragments arranged by size: 

                1965     803     761     332     309
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     475    3121    3597    4170 
   Size:        475    2646     476     573

  Fragments arranged by size: 

                2646     573     476     475
 
BstYI r'GATC_y

Cuts at:      0     558     890    1796    1887    1972    2640    2721    3582 
   Size:        558     332     906      91      85     668      81     861

Cuts at:   3582    4170 
   Size:        588

  Fragments arranged by size: 

                 906     861     668     588     558     332      91      85
                  81
 
Bsu36I CC'TnA_GG

Cuts at:      0      74    1235    1813    4170 
   Size:         74    1161     578    2357

  Fragments arranged by size: 

                2357    1161     578      74
 
BtgI C'CryG_G

Cuts at:      0    2079    2336    3821    3831    4170 
   Size:       2079     257    1485      10     339

  Fragments arranged by size: 

                2079    1485     339     257      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1838    4170 
   Size:       1838    2332
 
BtsI GCAGTG_nn'

Cuts at:      0     952    2225    2298    3101    4170 
   Size:        952    1273      73     803    1069

  Fragments arranged by size: 

                1273    1069     952     803      73
 
ClaI AT'CG_AT

Cuts at:      0    1364    4170 
   Size:       1364    2806
 
DraI TTT'AAA

Cuts at:      0     681    2763    4170 
   Size:        681    2082    1407

  Fragments arranged by size: 

                2082    1407     681
 
DraIII CAC_nnn'GTG

Cuts at:      0    1781    3718    3720    4170 
   Size:       1781    1937       2     450

  Fragments arranged by size: 

                1937    1781     450       2
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2847    4170 
   Size:       2847    1323
 
EaeI y'GGCC_r

Cuts at:      0      32    1157    1430    2115    2555    2559    3707    3923 
   Size:         32    1125     273     685     440       4    1148     216

Cuts at:   3923    4170 
   Size:        247

  Fragments arranged by size: 

                1148    1125     685     440     273     247     216      32
                   4
 
EagI C'GGCC_G

Cuts at:      0    2559    4170 
   Size:       2559    1611
 
EarI CTCTTCn'nnn_

Cuts at:      0    2857    4081    4170 
   Size:       2857    1224      89

  Fragments arranged by size: 

                2857    1224      89
 
EcoICRI GAG'CTC

Cuts at:      0     972    1329    3548    4170 
   Size:        972     357    2219     622

  Fragments arranged by size: 

                2219     972     622     357
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     276     283     394     565     716    1054    1440    1785 
   Size:        276       7     111     171     151     338     386     345

Cuts at:   1785    2149    2263    2278    3064    3169    4170 
   Size:        364     114      15     786     105    1001

  Fragments arranged by size: 

                1001     786     386     364     345     338     276     171
                 151     114     111     105      15       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2117    3577    4170 
   Size:       2117    1460     593

  Fragments arranged by size: 

                2117    1460     593
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1554    2447    4104    4124    4170 
   Size:       1554     893    1657      20      46

  Fragments arranged by size: 

                1657    1554     893      46      20
 
EcoRI G'AATT_C

Cuts at:      0    4147    4170 
   Size:       4147      23
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1600    1815    3379    4170 
   Size:       1600     215    1564     791

  Fragments arranged by size: 

                1600    1564     791     215
 
FseI GG_CCGG'CC

Cuts at:      0    2561    4170 
   Size:       2561    1609
 
HaeII r_GCGC'y

Cuts at:      0     482    4170 
   Size:        482    3688
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     796     828    1007    1039    1346    1378    2023    2055 
   Size:        796      32     179      32     307      32     645      32

Cuts at:   2055    2632    2664    3440    3472    3617    3649    4170 
   Size:        577      32     776      32     145      32     521

  Fragments arranged by size: 

                 796     776     645     577     521     307     179     145
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2577    4170 
   Size:       2577    1593
 
HindIII A'AGCT_T

Cuts at:      0    3670    4170 
   Size:       3670     500
 
KpnI G_GTAC'C

Cuts at:      0     494    4170 
   Size:        494    3676
 
MfeI C'AATT_G

Cuts at:      0    2090    4170 
   Size:       2090    2080
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1050    3896    4170 
   Size:       1050    2846     274

  Fragments arranged by size: 

                2846    1050     274
 
MscI TGG'CCA

Cuts at:      0      34    1159    1432    2117    3709    3925    4170 
   Size:         34    1125     273     685    1592     216     245

  Fragments arranged by size: 

                1592    1125     685     273     245     216      34
 
MslI CAynn'nnrTG

Cuts at:      0     473    3139    3823    4042    4170 
   Size:        473    2666     684     219     128

  Fragments arranged by size: 

                2666     684     473     219     128
 
MspA1I CmG'CkG

Cuts at:      0     694     942    1611    2485    2596    3006    4170 
   Size:        694     248     669     874     111     410    1164

  Fragments arranged by size: 

                1164     874     694     669     410     248     111
 
NaeI GCC'GGC

Cuts at:      0    2157    2559    4170 
   Size:       2157     402    1611

  Fragments arranged by size: 

                2157    1611     402
 
NcoI C'CATG_G

Cuts at:      0    2336    3821    4170 
   Size:       2336    1485     349

  Fragments arranged by size: 

                2336    1485     349
 
NgoMIV G'CCGG_C

Cuts at:      0    2155    2557    4170 
   Size:       2155     402    1613

  Fragments arranged by size: 

                2155    1613     402
 
NspI r_CATG'y

Cuts at:      0     145    2613    4170 
   Size:        145    2468    1557

  Fragments arranged by size: 

                2468    1557     145
 
PasI CC'CwG_GG

Cuts at:      0    3192    4170 
   Size:       3192     978
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     177    1007    2553    4170 
   Size:        177     830    1546    1617

  Fragments arranged by size: 

                1617    1546     830     177
 
PfoI T'CCnGG_A

Cuts at:      0      17    2303    4170 
   Size:         17    2286    1867

  Fragments arranged by size: 

                2286    1867      17
 
PmlI CAC'GTG

Cuts at:      0    3858    4170 
   Size:       3858     312
 
PpuMI rG'GwC_Cy

Cuts at:      0    2447    4104    4170 
   Size:       2447    1657      66

  Fragments arranged by size: 

                2447    1657      66
 
PstI C_TGCA'G

Cuts at:      0     941     947    2031    2210    2730    2900    3096    3569 
   Size:        941       6    1084     179     520     170     196     473

Cuts at:   3569    4170 
   Size:        601

  Fragments arranged by size: 

                1084     941     601     520     473     196     179     170
                   6
 
PvuII CAG'CTG

Cuts at:      0     694     942    2485    2596    3006    4170 
   Size:        694     248    1543     111     410    1164

  Fragments arranged by size: 

                1543    1164     694     410     248     111
 
SacI G_AGCT'C

Cuts at:      0     974    1331    3550    4170 
   Size:        974     357    2219     620

  Fragments arranged by size: 

                2219     974     620     357
 
SalI G'TCGA_C

Cuts at:      0    2575    4170 
   Size:       2575    1595
 
SanDI GG'GwC_CC

Cuts at:      0    4104    4170 
   Size:       4104      66
 
SapI GCTCTTCn'nnn_

Cuts at:      0    4081    4170 
   Size:       4081      89
 
SbfI CC_TGCA'GG

Cuts at:      0    2900    4170 
   Size:       2900    1270
 
ScaI AGT'ACT

Cuts at:      0    1870    4170 
   Size:       1870    2300
 
SfcI C'TryA_G

Cuts at:      0     937     943    2027    2206    2726    2896    3051    3092 
   Size:        937       6    1084     179     520     170     155      41

Cuts at:   3092    3565    4170 
   Size:        473     605

  Fragments arranged by size: 

                1084     937     605     520     473     179     170     155
                  41       6
 
SmlI C'TyrA_G

Cuts at:      0    1209    1322    1330    1660    2420    4170 
   Size:       1209     113       8     330     760    1750

  Fragments arranged by size: 

                1750    1209     760     330     113       8
 
SspI AAT'ATT

Cuts at:      0    1986    4170 
   Size:       1986    2184
 
StuI AGG'CCT

Cuts at:      0    1818    4170 
   Size:       1818    2352
 
StyI C'CwwG_G

Cuts at:      0    1685    1856    1962    2118    2336    3230    3590    3821 
   Size:       1685     171     106     156     218     894     360     231

Cuts at:   3821    4170 
   Size:        349

  Fragments arranged by size: 

                1685     894     360     349     231     218     171     156
                 106
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1014    1259    3040    3088    3151    4170 
   Size:       1014     245    1781      48      63    1019

  Fragments arranged by size: 

                1781    1019    1014     245      63      48
 
TatI w'GTAC_w

Cuts at:      0     122    1477    1868    2177    3062    3554    3665    3815 
   Size:        122    1355     391     309     885     492     111     150

Cuts at:   3815    4170 
   Size:        355

  Fragments arranged by size: 

                1355     885     492     391     355     309     150     122
                 111
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     256     288    1064    1096    1361    1393    4003    4035 
   Size:        256      32     776      32     265      32    2610      32

Cuts at:   4035    4170 
   Size:        135

  Fragments arranged by size: 

                2610     776     265     256     135      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      55     549    2496    2701    4170 
   Size:         55     494    1947     205    1469

  Fragments arranged by size: 

                1947    1469     494     205      55
 
XhoI C'TCGA_G

Cuts at:      0    2420    4170 
   Size:       2420    1750
 
XmnI GAAnn'nnTTC

Cuts at:      0     701    3038    3154    3359    4170 
   Size:        701    2337     116     205     811

  Fragments arranged by size: 

                2337     811     701     205     116
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AleI    AlwNI     ApoI     AvaI
    AvrII    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FseI    HaeII    Hin4I   HincII  HindIII     KpnI     MfeI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI     PstI    PvuII     SacI
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SmlI     SspI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     BaeI     BbeI
    BfrBI     BlpI    BmgBI     BmtI    BsaHI    BsiWI    BsrBI   BssHII
    BstBI   BstEII  BstZ17I    CspCI     EciI    EcoRV     FspI    FspAI
     HpaI     KasI     MluI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI
  Tth111I     XbaI     XmaI     ZraI
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