Restriction map for pF1KB3009
 (Linear) (Six-base) MAPSORT of: KIBB3009.seq  Check: 5713  from: 1  to: 1944



KIBB3009.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:21  ..

AccI GT'mk_AC

Cuts at:      0    1089    1944 
   Size:       1089     855
 
Acc65I G'GTAC_C

Cuts at:      0    1652    1944 
   Size:       1652     292
 
AclI AA'CG_TT

Cuts at:      0     712    1944 
   Size:        712    1232
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1463    1536    1620    1788    1872    1944 
   Size:       1463      73      84     168      84      72

  Fragments arranged by size: 

                1463     168      84      84      73      72
 
AlwNI CAG_nnn'CTG

Cuts at:      0      97     222     600     705    1944 
   Size:         97     125     378     105    1239

  Fragments arranged by size: 

                1239     378     125     105      97
 
ApoI r'AATT_y

Cuts at:      0      50    1040    1380    1944 
   Size:         50     990     340     564

  Fragments arranged by size: 

                 990     564     340      50
 
AvaI C'yCGr_G

Cuts at:      0     511     625    1944 
   Size:        511     114    1319

  Fragments arranged by size: 

                1319     511     114
 
BamHI G'GATC_C

Cuts at:      0     489    1944 
   Size:        489    1455
 
BanI G'GyrC_C

Cuts at:      0     555    1325    1652    1944 
   Size:        555     770     327     292

  Fragments arranged by size: 

                 770     555     327     292
 
BanII G_rGCy'C

Cuts at:      0     544     593     658    1944 
   Size:        544      49      65    1286

  Fragments arranged by size: 

                1286     544      65      49
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     812    1675    1944 
   Size:        812     863     269

  Fragments arranged by size: 

                 863     812     269
 
BbvCI CC'TCA_GC

Cuts at:      0    1682    1944 
   Size:       1682     262
 
BglII A'GATC_T

Cuts at:      0     546     857     993    1944 
   Size:        546     311     136     951

  Fragments arranged by size: 

                 951     546     311     136
 
Bme1580I G_kGCm'C

Cuts at:      0     558     626    1870    1944 
   Size:        558      68    1244      74

  Fragments arranged by size: 

                1244     558      74      68
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     613    1856    1944 
   Size:        613    1243      88

  Fragments arranged by size: 

                1243     613      88
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     205     672    1944 
   Size:        205     467    1272

  Fragments arranged by size: 

                1272     467     205
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     577     680    1003    1037    1532    1944 
   Size:        317     260     103     323      34     495     412

  Fragments arranged by size: 

                 495     412     323     317     260     103      34
 
Bpu10I CC'TnA_GC

Cuts at:      0    1682    1944 
   Size:       1682     262
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     902    1109    1770    1944 
   Size:        902     207     661     174

  Fragments arranged by size: 

                 902     661     207     174
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     219     381    1384    1944 
   Size:        219     162    1003     560

  Fragments arranged by size: 

                1003     560     219     162
 
BsaAI yAC'GTr

Cuts at:      0    1865    1944 
   Size:       1865      79
 
BsaWI w'CCGG_w

Cuts at:      0    1373    1664    1944 
   Size:       1373     291     280

  Fragments arranged by size: 

                1373     291     280
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     454     484     779     809    1944 
   Size:        454      30     295      30    1135

  Fragments arranged by size: 

                1135     454     295      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     358     454     601     666    1944 
   Size:        358      96     147      65    1278

  Fragments arranged by size: 

                1278     358     147      96      65
 
BseYI C'CCAG_C

Cuts at:      0     311    1944 
   Size:        311    1633
 
BsiHKAI G_wGCw'C

Cuts at:      0     593     658    1944 
   Size:        593      65    1286

  Fragments arranged by size: 

                1286     593      65
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     436    1944 
   Size:        436    1508
 
Bsp1286I G_dGCh'C

Cuts at:      0     544     558     593     626     658    1870    1944 
   Size:        544      14      35      33      32    1212      74

  Fragments arranged by size: 

                1212     544      74      35      33      32      14
 
BspHI T'CATG_A

Cuts at:      0    1188    1944 
   Size:       1188     756
 
BsrBI CCG'CTC

Cuts at:      0     460    1944 
   Size:        460    1484
 
BsrFI r'CCGG_y

Cuts at:      0     558    1944 
   Size:        558    1386
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1706    1944 
   Size:       1706     238
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     398    1944 
   Size:        398    1546
 
BstYI r'GATC_y

Cuts at:      0      30     251     489     546     857     993    1944 
   Size:         30     221     238      57     311     136     951

  Fragments arranged by size: 

                 951     311     238     221     136      57      30
 
Bsu36I CC'TnA_GG

Cuts at:      0     524     908    1246    1944 
   Size:        524     384     338     698

  Fragments arranged by size: 

                 698     524     384     338
 
BtgI C'CryG_G

Cuts at:      0      11    1944 
   Size:         11    1933
 
BtsI GCAGTG_nn'

Cuts at:      0     356    1944 
   Size:        356    1588
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1773    1808    1944 
   Size:       1773      35     136

  Fragments arranged by size: 

                1773     136      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1822    1944 
   Size:       1822     122
 
EaeI y'GGCC_r

Cuts at:      0      88     328     420     725    1944 
   Size:         88     240      92     305    1219

  Fragments arranged by size: 

                1219     305     240      92      88
 
EarI CTCTTCn'nnn_

Cuts at:      0      38     578     686     872     968    1382    1792    1944 
   Size:         38     540     108     186      96     414     410     152

  Fragments arranged by size: 

                 540     414     410     186     152     108      96      38
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     252    1944 
   Size:        252    1692
 
EcoICRI GAG'CTC

Cuts at:      0     591     656    1944 
   Size:        591      65    1288

  Fragments arranged by size: 

                1288     591      65
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     577     680    1003    1037    1463    1532    1536 
   Size:        317     260     103     323      34     426      69       4

Cuts at:   1536    1620    1788    1872    1944 
   Size:         84     168      84      72

  Fragments arranged by size: 

                 426     323     317     260     168     103      84      84
                  72      69      34       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     436    1767    1944 
   Size:        436    1331     177

  Fragments arranged by size: 

                1331     436     177
 
EcoO109I rG'GnC_Cy

Cuts at:      0     615     630     888    1772    1922    1944 
   Size:        615      15     258     884     150      22

  Fragments arranged by size: 

                 884     615     258     150      22      15
 
EcoRI G'AATT_C

Cuts at:      0      50    1944 
   Size:         50    1894
 
EcoRV GAT'ATC

Cuts at:      0     897    1900    1944 
   Size:        897    1003      44

  Fragments arranged by size: 

                1003     897      44
 
HaeII r_GCGC'y

Cuts at:      0    1716    1944 
   Size:       1716     228
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486     531     563     663     695     779     811 
   Size:        454      32      45      32     100      32      84      32

Cuts at:    811     844     876    1791    1823    1944 
   Size:         33      32     915      32     121

  Fragments arranged by size: 

                 915     454     121     100      84      45      33      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1571    1944 
   Size:       1571     373
 
HindIII A'AGCT_T

Cuts at:      0    1548    1884    1944 
   Size:       1548     336      60

  Fragments arranged by size: 

                1548     336      60
 
HpaI GTT'AAC

Cuts at:      0    1571    1944 
   Size:       1571     373
 
KpnI G_GTAC'C

Cuts at:      0    1656    1944 
   Size:       1656     288
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     171    1944 
   Size:        171    1773
 
MslI CAynn'nnrTG

Cuts at:      0    1863    1944 
   Size:       1863      81
 
MspA1I CmG'CkG

Cuts at:      0    1472    1944 
   Size:       1472     472
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     337    1944 
   Size:        337    1607
 
PmlI CAC'GTG

Cuts at:      0    1865    1944 
   Size:       1865      79
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1679    1711    1944 
   Size:       1679      32     233

  Fragments arranged by size: 

                1679     233      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     615     630     888    1922    1944 
   Size:        615      15     258    1034      22

  Fragments arranged by size: 

                1034     615     258      22      15
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1339    1371    1944 
   Size:       1339      32     573

  Fragments arranged by size: 

                1339     573      32
 
PstI C_TGCA'G

Cuts at:      0     488     605     648    1702    1944 
   Size:        488     117      43    1054     242

  Fragments arranged by size: 

                1054     488     242     117      43
 
SacI G_AGCT'C

Cuts at:      0     593     658    1944 
   Size:        593      65    1286

  Fragments arranged by size: 

                1286     593      65
 
SanDI GG'GwC_CC

Cuts at:      0     888    1944 
   Size:        888    1056
 
SapI GCTCTTCn'nnn_

Cuts at:      0     686     872    1792    1944 
   Size:        686     186     920     152

  Fragments arranged by size: 

                 920     686     186     152
 
SbfI CC_TGCA'GG

Cuts at:      0     605    1944 
   Size:        605    1339
 
SfcI C'TryA_G

Cuts at:      0       5     304     484     601     644    1440    1698    1944 
   Size:          5     299     180     117      43     796     258     246

  Fragments arranged by size: 

                 796     299     258     246     180     117      43       5
            
 
SmlI C'TyrA_G

Cuts at:      0     917    1124    1749    1944 
   Size:        917     207     625     195

  Fragments arranged by size: 

                 917     625     207     195
 
SspI AAT'ATT

Cuts at:      0    1119    1944 
   Size:       1119     825
 
StuI AGG'CCT

Cuts at:      0    1020    1944 
   Size:       1020     924
 
StyI C'CwwG_G

Cuts at:      0     391     883    1944 
   Size:        391     492    1061

  Fragments arranged by size: 

                1061     492     391
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     303     390    1738    1944 
   Size:        303      87    1348     206

  Fragments arranged by size: 

                1348     303     206      87
 
TatI w'GTAC_w

Cuts at:      0    1531    1944 
   Size:       1531     413
 
Tth111I GACn'n_nGTC

Cuts at:      0    1486    1944 
   Size:       1486     458
 
XmnI GAAnn'nnTTC

Cuts at:      0     278     778    1801    1858    1944 
   Size:        278     500    1023      57      86

  Fragments arranged by size: 

                1023     500     278      86      57
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AlwNI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI    BglII Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI
    BseYI  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrBI    BsrFI   BstAPI
    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MmeI     MslI
   MspA1I    PflMI     PmlI     PpiI    PpuMI     PsrI     PstI     SacI
    SanDI     SapI     SbfI     SfcI     SmlI     SspI     StuI     StyI
    TaqII     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmtI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BspMI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I    BtgZI     ClaI     DraI   DraIII     EagI     FalI     FseI
     FspI    FspAI     KasI     MfeI     MluI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI    PshAI     PsiI   PspOMI
    PspXI     PvuI    PvuII    RsrII    SacII     SalI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SwaI     TstI     XbaI     XcmI     XhoI     XmaI     ZraI
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