Restriction map for pF1KB1488
 (Circular) (Six-base) MAPSORT of: pF1KB1488.seq  Check: 7076  from: 1  to: 8345



pF1KB1488  8345 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2618    2754    2618 
   Size:        136    8209
 
AatII G_ACGT'C

Cuts at:   4571    4571 
   Size:       8345
 
AccI GT'mk_AC

Cuts at:   5383    5383 
   Size:       8345
 
Acc65I G'GTAC_C

Cuts at:    578    5361     578 
   Size:       4783    3562
 
AclI AA'CG_TT

Cuts at:   8185    8185 
   Size:       8345
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1376    1490    2023    2860    4261    4883    6170    6602    7349 
   Size:        114     533     837    1401     622    1287     432     747

Cuts at:   7349    7617    1376 
   Size:        268    2104

  Fragments arranged by size: 

                2104    1401    1287     837     747     622     533     432
                 268     114
 
AfeI AGC'GCT

Cuts at:   2209    2209 
   Size:       8345
 
AflIII A'CryG_T

Cuts at:   2230    2711    5697    6801    7778    2230 
   Size:        481    2986    1104     977    2797

  Fragments arranged by size: 

                2986    2797    1104     977     481
 
AgeI A'CCGG_T

Cuts at:   6733    6733 
   Size:       8345
 
AhdI GACnn_n'nnGTC

Cuts at:    468     468 
   Size:       8345
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1846    1878    1846 
   Size:         32    8313
 
AlwNI CAG_nnn'CTG

Cuts at:   1113    1303    1453    2769    3335    3808    5211    5650    7217 
   Size:        190     150    1316     566     473    1403     439    1567

Cuts at:   7217    1113 
   Size:       2241

  Fragments arranged by size: 

                2241    1567    1403    1316     566     473     439     190
                 150
 
ApaI G_GGCC'C

Cuts at:   1254    4667    1254 
   Size:       3413    4932
 
ApaLI G'TGCA_C

Cuts at:   4504    7115    4504 
   Size:       2611    5734
 
ApoI r'AATT_y

Cuts at:   2866    5349    7698    7954    2866 
   Size:       2483    2349     256    3257

  Fragments arranged by size: 

                3257    2483    2349     256
 
AseI AT'TA_AT

Cuts at:     20    2168      20 
   Size:       2148    6197
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8345
 
AvaI C'yCGr_G

Cuts at:    248     612    1516    1640    2852    3128    3223    4703    4792 
   Size:        364     904     124    1212     276      95    1480      89

Cuts at:   4792    5191    5365     248 
   Size:        399     174    3228

  Fragments arranged by size: 

                3228    1480    1212     904     399     364     276     174
                 124      95      89
 
BamHI G'GATC_C

Cuts at:   3272    5370    3272 
   Size:       2098    6247
 
BanI G'GyrC_C

Cuts at:    372     524     578     870    1719    1837    2772    2811    3673 
   Size:        152      54     292     849     118     935      39     862

Cuts at:   3673    3720    4470    4821    5330    5361    5775    6023    6058 
   Size:         47     750     351     509      31     414     248      35

Cuts at:   6058     372 
   Size:       2659

  Fragments arranged by size: 

                2659     935     862     849     750     509     414     351
                 292     248     152     118      54      47      39      35
                  31
 
BanII G_rGCy'C

Cuts at:    965    1178    1254    1673    1826    2300    2379    2785    3668 
   Size:        213      76     419     153     474      79     406     883

Cuts at:   3668    3802    4667    5359    6389     965 
   Size:        134     865     692    1030    2921

  Fragments arranged by size: 

                2921    1030     883     865     692     474     419     406
                 213     153     134      79      76
 
BbeI G_GCGC'C

Cuts at:   3724    4474    5779    6027    3724 
   Size:        750    1305     248    6042

  Fragments arranged by size: 

                6042    1305     750     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    991    1328    2131    3564    5015     991 
   Size:        337     803    1433    1451    4321

  Fragments arranged by size: 

                4321    1451    1433     803     337
 
BbvCI CC'TCA_GC

Cuts at:   4880    4880 
   Size:       8345
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   4542    4576    5105    5139    4542 
   Size:         34     529      34    7748

  Fragments arranged by size: 

                7748     529      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   6243    7004    8345    6243 
   Size:        761    1341    6243

  Fragments arranged by size: 

                6243    1341     761
 
BclI T'GATC_A

Cuts at:   5159    5865    5159 
   Size:        706    7639
 
BglI GCCn_nnn'nGGC

Cuts at:   1903    2363    3694    4746    1903 
   Size:        460    1331    1052    5502

  Fragments arranged by size: 

                5502    1331    1052     460
 
BglII A'GATC_T

Cuts at:    749    5860    7897     749 
   Size:       5111    2037    1197

  Fragments arranged by size: 

                5111    2037    1197
 
BlpI GC'TnA_GC

Cuts at:   1825    5457    1825 
   Size:       3632    4713
 
Bme1580I G_kGCm'C

Cuts at:    248     377     527    1254    2037    2816    3678    4508    4667 
   Size:        129     150     727     783     779     862     830     159

Cuts at:   4667    4824    4894    5292    5970    6063    7119     248 
   Size:        157      70     398     678      93    1056    1474

  Fragments arranged by size: 

                1474    1056     862     830     783     779     727     678
                 398     159     157     150     129      93      70
 
BmgBI CAC'GTC

Cuts at:   2233    4326    4671    2233 
   Size:       2093     345    5907

  Fragments arranged by size: 

                5907    2093     345
 
BmrI ACTGGGnnnn_n'

Cuts at:    789    1759    2572    2713    3804    5199    5557    5638    5973 
   Size:        970     813     141    1091    1395     358      81     335

Cuts at:   5973    8117     789 
   Size:       2144    1017

  Fragments arranged by size: 

                2144    1395    1091    1017     970     813     358     335
                 141      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3143    5262    6712    3143 
   Size:       2119    1450    4776

  Fragments arranged by size: 

                4776    2119    1450
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    220     352    1024    1090    1096    1133    1840    2401    2478 
   Size:        132     672      66       6      37     707     561      77

Cuts at:   2478    2674    3800    4189    4630    4933    5293     220 
   Size:        196    1126     389     441     303     360    3272

  Fragments arranged by size: 

                3272    1126     707     672     561     441     389     360
                 303     196     132      77      66      37       6
 
Bpu10I CC'TnA_GC

Cuts at:    334    2539    4880     334 
   Size:       2205    2341    3799

  Fragments arranged by size: 

                3799    2341    2205
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3138    5521    6400    6892    7190    3138 
   Size:       2383     879     492     298    4293

  Fragments arranged by size: 

                4293    2383     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1698    1751    2072    3220    4090    4648    5032    8049    1698 
   Size:         53     321    1148     870     558     384    3017    1994

  Fragments arranged by size: 

                3017    1994    1148     870     558     384     321      53
            
 
BsaAI yAC'GTr

Cuts at:   2522    2712    4641    5698    2522 
   Size:        190    1929    1057    5169

  Fragments arranged by size: 

                5169    1929    1057     190
 
BsaBI GATnn'nnATC

Cuts at:      6    5859    7896       6 
   Size:       5853    2037     455

  Fragments arranged by size: 

                5853    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1070    3721    4471    4568    5776    6024    6726    8222    1070 
   Size:       2651     750      97    1208     248     702    1496    1193

  Fragments arranged by size: 

                2651    1496    1208    1193     750     702     248      97
            
 
BsaWI w'CCGG_w

Cuts at:   1850    5534    6055    6733    7007    7154    7565    1850 
   Size:       3684     521     678     274     147     411    2630

  Fragments arranged by size: 

                3684    2630     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2486    2516    2716    2746    2870    2900    3204    3234    3828 
   Size:         30     200      30     124      30     304      30     594

Cuts at:   3828    3858    4168    4198    4480    4510    2486 
   Size:         30     310      30     282      30    6321

  Fragments arranged by size: 

                6321     594     310     304     282     200     124      30
                  30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    349     403     501    1180    1544    2410    2947    3836    4090 
   Size:         54      98     679     364     866     537     889     254

Cuts at:   4090    4186    4189    4501    5251     349 
   Size:         96       3     312     750    3443

  Fragments arranged by size: 

                3443     889     866     750     679     537     364     312
                 254      98      96      54       3
 
BseYI C'CCAG_C

Cuts at:   1296    1798    1908    2740    2759    3434    3661    3879    5291 
   Size:        502     110     832      19     675     227     218    1412

Cuts at:   5291    5770    6528    7105    7601    1296 
   Size:        479     758     577     496    2040

  Fragments arranged by size: 

                2040    1412     832     758     675     577     502     496
                 479     227     218     110      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    250     955    2296    2596    3608    3901    4750    4774    5044 
   Size:        705    1341     300    1012     293     849      24     270

Cuts at:   5044     250 
   Size:       3551

  Fragments arranged by size: 

                3551    1341    1012     849     705     300     293     270
                  24
 
BsiEI CG_ry'CG

Cuts at:    104    1540    5887    5933    7141     104 
   Size:       1436    4347      46    1208    1308

  Fragments arranged by size: 

                4347    1436    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    619    1178    1826    3214    4508    5269    5359    6137    6327 
   Size:        559     648    1388    1294     761      90     778     190

Cuts at:   6327    7119     619 
   Size:        792    1845

  Fragments arranged by size: 

                1845    1388    1294     792     778     761     648     559
                 190      90
 
BsmI GAATG_Cn'

Cuts at:   1307    1403    1307 
   Size:         96    8249
 
Bsp1286I G_dGCh'C

Cuts at:    248     377     527     619     965    1178    1254    1673    1826 
   Size:        129     150      92     346     213      76     419     153

Cuts at:   1826    2037    2300    2379    2785    2816    3214    3668    3678 
   Size:        211     263      79     406      31     398     454      10

Cuts at:   3678    3802    4508    4667    4824    4894    5269    5292    5359 
   Size:        124     706     159     157      70     375      23      67

Cuts at:   5359    5970    6063    6137    6327    6389    7119     248 
   Size:        611      93      74     190      62     730    1474

  Fragments arranged by size: 

                1474     730     706     611     454     419     406     398
                 375     346     263     213     211     190     159     157
                 153     150     129     124      93      92      79      76
                  74      70      67      62      31      23      10
 
BspEI T'CCGG_A

Cuts at:   7565    7565 
   Size:       8345
 
BspHI T'CATG_A

Cuts at:   1658    7521    1658 
   Size:       5863    2482
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    509    2618    2754    3372    3740    4294    5219    5395    5609 
   Size:       2109     136     618     368     554     925     176     214

Cuts at:   5609    5911    6292     509 
   Size:        302     381    2562

  Fragments arranged by size: 

                2562    2109     925     618     554     381     368     302
                 214     176     136
 
BsrBI CCG'CTC

Cuts at:     50    1391    1536    4917    6637    6691    8175    8340      50 
   Size:       1341     145    3381    1720      54    1484     165      55

  Fragments arranged by size: 

                3381    1720    1484    1341     165     145      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   3516    3770    3828    6257    3516 
   Size:        254      58    2429    5604

  Fragments arranged by size: 

                5604    2429     254      58
 
BsrFI r'CCGG_y

Cuts at:   4740    4758    6343    6524    6733    4740 
   Size:         18    1585     181     209    6352

  Fragments arranged by size: 

                6352    1585     209     181      18
 
BsrGI T'GTAC_A

Cuts at:    680     680 
   Size:       8345
 
BssSI C'ACGA_G

Cuts at:   1556    3931    6616    6974    1556 
   Size:       2375    2685     358    2927

  Fragments arranged by size: 

                2927    2685    2375     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3924    4737    5287    5722    3924 
   Size:        813     550     435    6547

  Fragments arranged by size: 

                6547     813     550     435
 
BstBI TT'CG_AA

Cuts at:   2864    6706    2864 
   Size:       3842    4503
 
BstXI CCAn_nnnn'nTGG

Cuts at:    743    2450    3778    3887    4482    5317     743 
   Size:       1707    1328     109     595     835    3771

  Fragments arranged by size: 

                3771    1707    1328     835     595     109
 
BstYI r'GATC_y

Cuts at:      1     749    1215    2384    2528    3057    3246    3272    5370 
   Size:        748     466    1169     144     529     189      26    2098

Cuts at:   5370    5837    5860    6195    6441    7453    7539    7551    7897 
   Size:        467      23     335     246    1012      86      12     346

Cuts at:   7897       1 
   Size:        449

  Fragments arranged by size: 

                2098    1169    1012     748     529     467     466     449
                 346     335     246     189     144      86      26      23
                  12
 
Bsu36I CC'TnA_GG

Cuts at:    425     425 
   Size:       8345
 
BtgI C'CryG_G

Cuts at:    451    1328    1592    1752    2532    2816    3242     451 
   Size:        877     264     160     780     284     426    5554

  Fragments arranged by size: 

                5554     877     780     426     284     264     160
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3531    4545    6295    6476    6639    3531 
   Size:       1014    1750     181     163    5237

  Fragments arranged by size: 

                5237    1750    1014     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1605    3211    3214    5588    1605 
   Size:       1606       3    2374    4362

  Fragments arranged by size: 

                4362    2374    1606       3
 
DraI TTT'AAA

Cuts at:   5344    5344 
   Size:       8345
 
DraIII CAC_nnn'GTG

Cuts at:   1242    1767    3243    4517    4853    1242 
   Size:        525    1476    1274     336    4734

  Fragments arranged by size: 

                4734    1476    1274     525     336
 
DrdI GACnn_nn'nnGTC

Cuts at:   2111    6051    6909    2111 
   Size:       3940     858    3547

  Fragments arranged by size: 

                3940    3547     858
 
EaeI y'GGCC_r

Cuts at:    564    1392    1537    1769    2570    3566    4556    4760    4844 
   Size:        828     145     232     801     996     990     204      84

Cuts at:   4844    5930    6104    6495    6522    7867     564 
   Size:       1086     174     391      27    1345    1042

  Fragments arranged by size: 

                1345    1086    1042     996     990     828     801     391
                 232     204     174     145      84      27
 
EagI C'GGCC_G

Cuts at:   1537    5930    1537 
   Size:       4393    3952
 
EarI CTCTTCn'nnn_

Cuts at:    385     511    2335    6368    6578     385 
   Size:        126    1824    4033     210    2152

  Fragments arranged by size: 

                4033    2152    1824     210     126
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2608    2626    2762    3307    4542    5017    6553    6863    7009 
   Size:         18     136     545    1235     475    1536     310     146

Cuts at:   7009    8155    2608 
   Size:       1146    2798

  Fragments arranged by size: 

                2798    1536    1235    1146     545     475     310     146
                 136      18
 
EcoICRI GAG'CTC

Cuts at:   1176    1824    5357    1176 
   Size:        648    3533    4164

  Fragments arranged by size: 

                4164    3533     648
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    220     352    1024    1090    1096    1133    1376    1490    1840 
   Size:        132     672      66       6      37     243     114     350

Cuts at:   1840    2023    2401    2478    2674    2860    3800    4189    4261 
   Size:        183     378      77     196     186     940     389      72

Cuts at:   4261    4630    4883    4933    5293    6170    6602    7349    7617 
   Size:        369     253      50     360     877     432     747     268

Cuts at:   7617     220 
   Size:        948

  Fragments arranged by size: 

                 948     940     877     747     672     432     389     378
                 369     360     350     268     253     243     196     186
                 183     132     114      77      72      66      50      37
                   6
 
EcoNI CCTnn'n_nnAGG

Cuts at:    970    1051    1264    1508    2392    4171     970 
   Size:         81     213     244     884    1779    5144

  Fragments arranged by size: 

                5144    1779     884     244     213      81
 
EcoO109I rG'GnC_Cy

Cuts at:    210     421     740    1250    1251    1513    1679    1802    2400 
   Size:        211     319     510       1     262     166     123     598

Cuts at:   2400    2858    3086    3360    3462    3876    4245    4293    4663 
   Size:        458     228     274     102     414     369      48     370

Cuts at:   4663    4664    5484     210 
   Size:          1     820    3071

  Fragments arranged by size: 

                3071     820     598     510     458     414     370     369
                 319     274     262     228     211     166     123     102
                  48       1       1
 
EcoRI G'AATT_C

Cuts at:   2866    5349    2866 
   Size:       2483    5862
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    342    1008    1893    6364     342 
   Size:        666     885    4471    2323

  Fragments arranged by size: 

                4471    2323     885     666
 
FspI TGC'GCA

Cuts at:   2335    2431    5101    6126    2335 
   Size:         96    2670    1025    4554

  Fragments arranged by size: 

                4554    2670    1025      96
 
HaeII r_GCGC'y

Cuts at:   1909    2211    3724    4474    5779    6027    7049    8028    1909 
   Size:        302    1513     750    1305     248    1022     979    2226

  Fragments arranged by size: 

                2226    1513    1305    1022     979     750     302     248
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    454     486    1165    1197    1634    1666    1775    1807    2872 
   Size:         32     679      32     437      32     109      32    1065

Cuts at:   2872    2904    4027    4059    4480    4512    5151    5183    6187 
   Size:         32    1123      32     421      32     639      32    1004

Cuts at:   6187    6217    6219    6249     454 
   Size:         30       2      30    2550

  Fragments arranged by size: 

                2550    1123    1065    1004     679     639     437     421
                 109      32      32      32      32      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   5384    5384 
   Size:       8345
 
KasI G'GCGC_C

Cuts at:   3720    4470    5775    6023    3720 
   Size:        750    1305     248    6042

  Fragments arranged by size: 

                6042    1305     750     248
 
KpnI G_GTAC'C

Cuts at:    582    5365     582 
   Size:       4783    3562
 
MluI A'CGCG_T

Cuts at:   7778    7778 
   Size:       8345
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    806    2394    3190    3884    4426    7016    7200     806 
   Size:       1588     796     694     542    2590     184    1951

  Fragments arranged by size: 

                2590    1951    1588     796     694     542     184
 
MscI TGG'CCA

Cuts at:    566    1771    2572    4558    4846    6106     566 
   Size:       1205     801    1986     288    1260    2805

  Fragments arranged by size: 

                2805    1986    1260    1205     801     288
 
MslI CAynn'nnrTG

Cuts at:    634     884    2425    3763    6334    6461     634 
   Size:        250    1541    1338    2571     127    2518

  Fragments arranged by size: 

                2571    2518    1541    1338     250     127
 
MspA1I CmG'CkG

Cuts at:    435     570    2056    2262    2652    2830    3685    4143    4434 
   Size:        135    1486     206     390     178     855     458     291

Cuts at:   4434    5086    5277    5456    5770    6130    7143    7388     435 
   Size:        652     191     179     314     360    1013     245    1392

  Fragments arranged by size: 

                1486    1392    1013     855     652     458     390     360
                 314     291     245     206     191     179     178     135
            
 
NaeI GCC'GGC

Cuts at:   4760    6526    4760 
   Size:       1766    6579
 
NarI GG'CG_CC

Cuts at:   3721    4471    5776    6024    3721 
   Size:        750    1305     248    6042

  Fragments arranged by size: 

                6042    1305     750     248
 
NcoI C'CATG_G

Cuts at:    451    1752    2532     451 
   Size:       1301     780    6264

  Fragments arranged by size: 

                6264    1301     780
 
NdeI CA'TA_TG

Cuts at:    948     948 
   Size:       8345
 
NgoMIV G'CCGG_C

Cuts at:   4758    6524    4758 
   Size:       1766    6579
 
NspI r_CATG'y

Cuts at:    687    3270    3492    5398    5898    6805     687 
   Size:       2583     222    1906     500     907    2227

  Fragments arranged by size: 

                2583    2227    1906     907     500     222
 
PciI A'CATG_T

Cuts at:   6801    6801 
   Size:       8345
 
PflMI CCAn_nnn'nTGG

Cuts at:    438    1303    1716    1767    3243     438 
   Size:        865     413      51    1476    5540

  Fragments arranged by size: 

                5540    1476     865     413      51
 
PfoI T'CCnGG_A

Cuts at:    236     701    1064    1361    3458    3575    4265    5309     236 
   Size:        465     363     297    2097     117     690    1044    3272

  Fragments arranged by size: 

                3272    2097    1044     690     465     363     297     117
            
 
PmeI GTTT'AAAC

Cuts at:   5344    5344 
   Size:       8345
 
PmlI CAC'GTG

Cuts at:   2522    2712    2522 
   Size:        190    8155
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   7484    7516    7484 
   Size:         32    8313
 
PpuMI rG'GwC_Cy

Cuts at:    210     740    1679    2400    2858    3360    3462    4245    4293 
   Size:        530     939     721     458     502     102     783      48

Cuts at:   4293     210 
   Size:       4262

  Fragments arranged by size: 

                4262     939     783     721     530     502     458     102
                  48
 
PshAI GACnn'nnGTC

Cuts at:   1690    3467    1690 
   Size:       1777    6568
 
PspOMI G'GGCC_C

Cuts at:   1250    4663    1250 
   Size:       3413    4932
 
PspXI vC'TCGA_Gb

Cuts at:   2852    3128    5191    2852 
   Size:        276    2063    6006

  Fragments arranged by size: 

                6006    2063     276
 
PstI C_TGCA'G

Cuts at:    164    1134    1268    1442    1458    1941    2393    2903    3410 
   Size:        970     134     174      16     483     452     510     507

Cuts at:   3410    4142    4184    4415    4433    4928    5392    6077     164 
   Size:        732      42     231      18     495     464     685    2432

  Fragments arranged by size: 

                2432     970     732     685     510     507     495     483
                 464     452     231     174     134      42      18      16
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       8345
 
PvuII CAG'CTG

Cuts at:    435    2652    3685    4143    4434    5770    6130     435 
   Size:       2217    1033     458     291    1336     360    2650

  Fragments arranged by size: 

                2650    2217    1336    1033     458     360     291
 
RsrII CG'GwC_CG

Cuts at:   6540    6540 
   Size:       8345
 
SacI G_AGCT'C

Cuts at:   1178    1826    5359    1178 
   Size:        648    3533    4164

  Fragments arranged by size: 

                4164    3533     648
 
SalI G'TCGA_C

Cuts at:   5382    5382 
   Size:       8345
 
SanDI GG'GwC_CC

Cuts at:    210     210 
   Size:       8345
 
SapI GCTCTTCn'nnn_

Cuts at:    385     511    2335    6368    6578     385 
   Size:        126    1824    4033     210    2152

  Fragments arranged by size: 

                4033    2152    1824     210     126
 
SbfI CC_TGCA'GG

Cuts at:   2393    5392    2393 
   Size:       2999    5346
 
ScaI AGT'ACT

Cuts at:   7862    7862 
   Size:       8345
 
SfcI C'TryA_G

Cuts at:     33     160    1130    1264    1438    1454    1937    2389    2899 
   Size:        127     970     134     174      16     483     452     510

Cuts at:   2899    3406    4138    4180    4297    4411    4429    4924    4941 
   Size:        507     732      42     117     114      18     495      17

Cuts at:   4941    5388    6073    7066    7257      33 
   Size:        447     685     993     191    1121

  Fragments arranged by size: 

                1121     993     970     732     685     510     507     495
                 483     452     447     191     174     134     127     117
                 114      42      18      17      16
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2363    2363 
   Size:       8345
 
SfoI GGC'GCC

Cuts at:   3722    4472    5777    6025    3722 
   Size:        750    1305     248    6042

  Fragments arranged by size: 

                6042    1305     750     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8345
 
SmaI CCC'GGG

Cuts at:   1518    4705    5367    1518 
   Size:       3187     662    4496

  Fragments arranged by size: 

                4496    3187     662
 
SmlI C'TyrA_G

Cuts at:   2852    3117    3128    5191    5500    6415    6907    7169    2852 
   Size:        265      11    2063     309     915     492     262    4028

  Fragments arranged by size: 

                4028    2063     915     492     309     265     262      11
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       8345
 
SphI G_CATG'C

Cuts at:   3492    5398    5898    3492 
   Size:       1906     500    5939

  Fragments arranged by size: 

                5939    1906     500
 
SspI AAT'ATT

Cuts at:   2489    5155    2489 
   Size:       2666    5679
 
StuI AGG'CCT

Cuts at:   1270    2561    1270 
   Size:       1291    7054
 
StyI C'CwwG_G

Cuts at:    451    1211    1271    1752    1916    2117    2532    2891    2957 
   Size:        760      60     481     164     201     415     359      66

Cuts at:   2957    2978    3230    3716    3993    4190    4388    4475    5069 
   Size:         21     252     486     277     197     198      87     594

Cuts at:   5069    5479    5833     451 
   Size:        410     354    2963

  Fragments arranged by size: 

                2963     760     594     486     481     415     410     359
                 354     277     252     201     198     197     164      87
                  66      60      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    808    1680    1778    3661    4672    4680    5065    5928    6061 
   Size:        872      98    1883    1011       8     385     863     133

Cuts at:   6061    6520    6729     808 
   Size:        459     209    2424

  Fragments arranged by size: 

                2424    1883    1011     872     863     459     385     209
                 133      98       8
 
TatI w'GTAC_w

Cuts at:    680    3791    4331    7860     680 
   Size:       3111     540    3529    1165

  Fragments arranged by size: 

                3529    3111    1165     540
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    245     277    2486    2518    2716    2748    2870    2902    3204 
   Size:         32    2209      32     198      32     122      32     302

Cuts at:   3204    3236    3828    3860    7862    7894     245 
   Size:         32     592      32    4002      32     696

  Fragments arranged by size: 

                4002    2209     696     592     302     198     122      32
                  32      32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   4690    6142    4690 
   Size:       1452    6893
 
XbaI T'CTAG_A

Cuts at:   5376    5376 
   Size:       8345
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    633    1488    3156    3723    5840     633 
   Size:        855    1668     567    2117    3138

  Fragments arranged by size: 

                3138    2117    1668     855     567
 
XhoI C'TCGA_G

Cuts at:   2852    3128    5191    2852 
   Size:        276    2063    6006

  Fragments arranged by size: 

                6006    2063     276
 
XmaI C'CCGG_G

Cuts at:   1516    4703    5365    1516 
   Size:       3187     662    4496

  Fragments arranged by size: 

                4496    3187     662
 
XmnI GAAnn'nnTTC

Cuts at:    988    4120     988 
   Size:       3132    5213
 
ZraI GAC'GTC

Cuts at:   4569    4569 
   Size:       8345
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

    AflII     AleI     AlfI     AscI    AvrII     BaeI    BfrBI     BmtI
    BsiWI    BsmBI   BssHII   BstEII  BstZ17I     ClaI    CspCI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PsiI     PsrI    SacII    SexAI    SgrAI
    SnaBI     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com