Restriction map for pF1KB0063
##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

Acc65I      AclI        AflII       Alw44I      Aor51HI     
ApaLI       AscI        Asp718I     AspI        AviII       
AvrII       BbeI        BbrPI       BciVI       BfrI        
BglII       BlnI        BsaBI       BsiWI       BspHI       
BsrBI       BssHII      Bst1107I    BstZ17I     CpoI        
DraI        Eco47III    EcoT22I     FseI        FspI        
KasI        KpnI        MamI        MluI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PflFI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        SfoI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tth111I     


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        2022  GACGTC               GACGT^C
AcyI         2022  GACGTC               G[GA]^CG[CT]C
AgeI         5743  ACCGGT               A^CCGGT
AseI         4618  ATTAAT               AT^TAAT
AsnI         4618  ATTAAT               AT^TAAT
Asp700I       253  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI         2459  TGATCA               T^GATCA
BlpI          366  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      366  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2127  CACGTA               [CT]AC^GT[GA]
BsaHI        2022  GACGTC               G[GA]^CG[CT]C
Bsp1407I     1964  TGTACA               T^GTACA
BsrGI        1964  TGTACA               T^GTACA
BstBI         715  TTCGAA               TT^CGAA
CelII         366  GCTGAGC              GC^T[ACGT]AGC
DraIII       2350  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         5479  GACAGTTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1431  CGGCCG               C^GGCCG
EclXI        1431  CGGCCG               C^GGCCG
Eco52I       1431  CGGCCG               C^GGCCG
EcoRV        2340  GATATC               GAT^ATC
FbaI         2459  TGATCA               T^GATCA
Hin1I        2022  GACGTC               G[GA]^CG[CT]C
HindIII       255  AAGCTT               A^AGCTT
HpaI         6499  GTTAAC               GTT^AAC
KspI         3705  CCGCGG               CCGC^GG
NdeI         6125  CATATG               CA^TATG
NspV          715  TTCGAA               TT^CGAA
PinAI        5743  ACCGGT               A^CCGGT
PshAI        3647  GACTGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4618  ATTAAT               AT^TAAT
SacII        3705  CCGCGG               CCGC^GG
SfiI         5401  GGCCCATCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          715  TTCGAA               TT^CGAA
SspBI        1964  TGTACA               T^GTACA
XbaI         2202  TCTAGA               T^CTAGA
XmnI          253  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI         1162  GTAGAC               GT^[AC][GT]AC
             4516  GTCTAC              
AccII        3706  CGCG                 CG^CG
             6097  CGCG                
BamHI         181  GGATCC               G^GATCC
             2196  GGATCC              
BsmI         4659  GAATGC               GAATGC(1/-1)
             2102  GCATTC              
BspDI        2488  ATCGAT               AT^CGAT
             2545  ATCGAT              
BssSI        1741  CACGAG               CACGAG(-5/-1)
             1978  CACGAG              
BstAPI       1664  GCAAAGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             6124  GCATATGCTGC         
BstEII       2391  GGTGACC              G^GT[ACGT]ACC
             5746  GGTTACC             
BstPI        2391  GGTGACC              G^GT[ACGT]ACC
             5746  GGTTACC             
BstUI        3706  CGCG                 CG^CG
             6097  CGCG                
ClaI         2488  ATCGAT               AT^CGAT
             2545  ATCGAT              
EcoO65I      2391  GGTGACC              G^GT[ACGT]ACC
             5746  GGTTACC             
EcoRI        4720  GAATTC               G^AATTC
             6157  GAATTC              
HaeII        2113  AGCGCC               [GA]GCGC^[CT]
             6024  GGCGCT              
MfeI          858  CAATTG               C^AATTG
             5918  CAATTG              
MunI          858  CAATTG               C^AATTG
             5918  CAATTG              
MvnI         3706  CGCG                 CG^CG
             6097  CGCG                
PaeR7I       4219  CTCGAG               C^TCGAG
             5569  CTCGAG              
PflMI         135  CCAGCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3072  CCAGTGGGTGG         
PpuMI          68  GGGTCCT              [GA]G^G[AT]CC[CT]
             2966  AGGACCT             
SapI         2097  GAAGAGC              GCTCTTC(1/4)
             5622  GAAGAGC             
Van91I        135  CCAGCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3072  CCAGTGGGTGG         
XhoI         4219  CTCGAG               C^TCGAG
             5569  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccIII      AciI        AcsI        AfaI        AflIII      
AhdI        AluI        AlwI        AlwNI       AocI        
ApaI        ApoI        AspEI       AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcgI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspEI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
Eam1105I    EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MroI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScaI        ScrFI       SexAI       SfaNI       
SfcI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
XmaCI       XmaI        
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