Restriction map for pF1KB0063
 (Circular) (Six-base) MAPSORT of: pF1KB0063.seq  Check: 8201  from: 1  to: 9716



pF1KB0063  9716 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    124    1102    1150    6427     124 
   Size:        978      48    5277    3413

  Fragments arranged by size: 

                5277    3413     978      48
 
AatII G_ACGT'C

Cuts at:   2134    2134 
   Size:       9716
 
AccI GT'mk_AC

Cuts at:   1271    4625    6754    1271 
   Size:       3354    2129    4233

  Fragments arranged by size: 

                4233    3354    2129
 
Acc65I G'GTAC_C

Cuts at:   6732    6732 
   Size:       9716
 
AclI AA'CG_TT

Cuts at:   9556    9556 
   Size:       9716
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    332     591     635    2273    2512    2716    3064    3816    6430 
   Size:        259      44    1638     239     204     348     752    2614

Cuts at:   6430    6663    7541    7973    8720    8988     332 
   Size:        233     878     432     747     268    1060

  Fragments arranged by size: 

                2614    1638    1060     878     752     747     432     348
                 268     259     239     233     204      44
 
AflIII A'CryG_T

Cuts at:    892    2744    2808    3303    7068    8172    9149     892 
   Size:       1852      64     495    3765    1104     977    1459

  Fragments arranged by size: 

                3765    1852    1459    1104     977     495      64
 
AgeI A'CCGG_T

Cuts at:   5851    8104    5851 
   Size:       2253    7463
 
AhdI GACnn_n'nnGTC

Cuts at:   1654    1918    2130    1654 
   Size:        264     212    9240

  Fragments arranged by size: 

                9240     264     212
 
AleI CACnn'nnGTG

Cuts at:   1256    2090    1256 
   Size:        834    8882
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    545     545 
   Size:       9716
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2092    2124    2665    2697    6626    6658    2092 
   Size:         32     541      32    3929      32    5150

  Fragments arranged by size: 

                5150    3929     541      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    457     779    1619    1805    1898    6551    7021    8588     457 
   Size:        322     840     186      93    4653     470    1567    1585

  Fragments arranged by size: 

                4653    1585    1567     840     470     322     186      93
            
 
ApaI G_GGCC'C

Cuts at:   2485    5726    5990    2485 
   Size:       3241     264    6211

  Fragments arranged by size: 

                6211    3241     264
 
ApaLI G'TGCA_C

Cuts at:   8486    8486 
   Size:       9716
 
ApoI r'AATT_y

Cuts at:   2263    3139    4828    5277    6265    6720    9069    9325    2263 
   Size:        876    1689     449     988     455    2349     256    2654

  Fragments arranged by size: 

                2654    2349    1689     988     876     455     449     256
            
 
AseI AT'TA_AT

Cuts at:     20    4727      20 
   Size:       4707    5009
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9716
 
AvaI C'yCGr_G

Cuts at:   1923    2477    4320    4327    4459    5677    5837    6736    1923 
   Size:        554    1843       7     132    1218     160     899    4903

  Fragments arranged by size: 

                4903    1843    1218     899     554     160     132       7
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   6022    6055    6022 
   Size:         33    9683
 
BamHI G'GATC_C

Cuts at:    289    2304    6741     289 
   Size:       2015    4437    3264

  Fragments arranged by size: 

                4437    3264    2015
 
BanI G'GyrC_C

Cuts at:    339    1090    5301    6732    7146    7394    7429     339 
   Size:        751    4211    1431     414     248      35    2626

  Fragments arranged by size: 

                4211    2626    1431     751     414     248      35
 
BanII G_rGCy'C

Cuts at:    356     671    1046    1325    2485    3782    5242    5726    5737 
   Size:        315     375     279    1160    1297    1460     484      11

Cuts at:   5737    5947    5990    6323    6730    7760     356 
   Size:        210      43     333     407    1030    2312

  Fragments arranged by size: 

                2312    1460    1297    1160    1030     484     407     375
                 333     315     279     210      43      11
 
BbeI G_GCGC'C

Cuts at:   7150    7398    7150 
   Size:        248    9468
 
BbsI GAAGACnn'nnnn_

Cuts at:    396     500    1037    1130    1595    1967    3023    3130    5037 
   Size:        104     537      93     465     372    1056     107    1907

Cuts at:   5037    6135     396 
   Size:       1098    3977

  Fragments arranged by size: 

                3977    1907    1098    1056     537     465     372     107
                 104      93
 
BbvCI CC'TCA_GC

Cuts at:   2915    3581    4400    6128    2915 
   Size:        666     819    1728    6503

  Fragments arranged by size: 

                6503    1728     819     666
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    686     720     804     838     910     944     686 
   Size:         34      84      34      72      34    9458

  Fragments arranged by size: 

                9458      84      72      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   7614    8375    9716    7614 
   Size:        761    1341    7614

  Fragments arranged by size: 

                7614    1341     761
 
BclI T'GATC_A

Cuts at:   2567    7236    2567 
   Size:       4669    5047
 
BglI GCCn_nnn'nGGC

Cuts at:    112     923    1853    2230    5516     112 
   Size:        811     930     377    3286    4312

  Fragments arranged by size: 

                4312    3286     930     811     377
 
BglII A'GATC_T

Cuts at:   7231    9268    7231 
   Size:       2037    7679
 
BlpI GC'TnA_GC

Cuts at:    475    6828     475 
   Size:       6353    3363
 
Bme1580I G_kGCm'C

Cuts at:    872    2485    5304    5345    5726    5990    7341    7434    8490 
   Size:       1613    2819      41     381     264    1351      93    1056

Cuts at:   8490     872 
   Size:       2098

  Fragments arranged by size: 

                2819    2098    1613    1351    1056     381     264      93
                  41
 
BmgBI CAC'GTC

Cuts at:   2631    2809    2631 
   Size:        178    9538
 
BmrI ACTGGGnnnn_n'

Cuts at:    288     859    1406    1793    1886    6928    7009    7344    9488 
   Size:        571     547     387      93    5042      81     335    2144

Cuts at:   9488     288 
   Size:        516

  Fragments arranged by size: 

                5042    2144     571     547     516     387     335      93
                  81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   8083    8083 
   Size:       9716
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    331     505     514     535    1325    2173    2215    2921    3653 
   Size:        174       9      21     790     848      42     706     732

Cuts at:   3653    4564    4879    5316     331 
   Size:        911     315     437    4731

  Fragments arranged by size: 

                4731     911     848     790     732     706     437     315
                 174      42      21       9
 
Bpu10I CC'TnA_GC

Cuts at:   2915    3332    3581    4400    5941    6128    2915 
   Size:        417     249     819    1541     187    6503

  Fragments arranged by size: 

                6503    1541     819     417     249     187
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    166    1309    3493    4959    6892    7771    8263    8561     166 
   Size:       1143    2184    1466    1933     879     492     298    1321

  Fragments arranged by size: 

                2184    1933    1466    1321    1143     879     492     298
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   2395    2633    2761    3094    4498    4908    5149    6660    9420 
   Size:        238     128     333    1404     410     241    1511    2760

Cuts at:   9420    2395 
   Size:       2691

  Fragments arranged by size: 

                2760    2691    1511    1404     410     333     241     238
                 128
 
BsaAI yAC'GTr

Cuts at:   2237    7069    2237 
   Size:       4832    4884
 
BsaBI GATnn'nnATC

Cuts at:      6    7230    9267       6 
   Size:       7224    2037     455

  Fragments arranged by size: 

                7224    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2131    7147    7395    8097    9593    2131 
   Size:       5016     248     702    1496    2254

  Fragments arranged by size: 

                5016    2254    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2345    4652    4968    5327    5851    6905    7426    8104    8378 
   Size:       2307     316     359     524    1054     521     678     274

Cuts at:   8378    8525    8936    2345 
   Size:        147     411    3125

  Fragments arranged by size: 

                3125    2307    1054     678     524     521     411     359
                 316     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    985    1015    1171    1201    1312    1342    2665    2695    3605 
   Size:         30     156      30     111      30    1323      30     910

Cuts at:   3605    3635     985 
   Size:         30    7066

  Fragments arranged by size: 

                7066    1323     910     156     111      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    502     895    1192    1970    2406    3883    6009     502 
   Size:        393     297     778     436    1477    2126    4209

  Fragments arranged by size: 

                4209    2126    1477     778     436     393     297
 
BseYI C'CCAG_C

Cuts at:    355     772    2524    2757    3365    3638    3911    4155    4184 
   Size:        417    1752     233     608     273     273     244      29

Cuts at:   4184    4645    7141    7899    8476    8972     355 
   Size:        461    2496     758     577     496    1099

  Fragments arranged by size: 

                2496    1752    1099     758     608     577     496     461
                 417     273     273     244     233      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1709    2231    2440    3214    3451    3724    4069    4270    1709 
   Size:        522     209     774     237     273     345     201    7155

  Fragments arranged by size: 

                7155     774     522     345     273     237     209     201
            
 
BsiEI CG_ry'CG

Cuts at:    104     597    1071    1542    7258    7304    8512     104 
   Size:        493     474     471    5716      46    1208    1308

  Fragments arranged by size: 

                5716    1308    1208     493     474     471      46
 
BsiHKAI G_wGCw'C

Cuts at:    377     887    1046    1325    3416    3575    4235    5737    6323 
   Size:        510     159     279    2091     159     660    1502     586

Cuts at:   6323    6519    6730    7508    7698    8490     377 
   Size:        196     211     778     190     792    1603

  Fragments arranged by size: 

                2091    1603    1502     792     778     660     586     510
                 279     211     196     190     159     159
 
BsmI GAATG_Cn'

Cuts at:   2210    4773    2210 
   Size:       2563    7153
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1926    2011    6183    1926 
   Size:         85    4172    5459

  Fragments arranged by size: 

                5459    4172      85
 
Bsp1286I G_dGCh'C

Cuts at:    356     377     671     872     887    1046    1325    2485    3416 
   Size:         21     294     201      15     159     279    1160     931

Cuts at:   3416    3575    3782    4235    5242    5304    5345    5726    5737 
   Size:        159     207     453    1007      62      41     381      11

Cuts at:   5737    5947    5990    6323    6519    6730    7341    7434    7508 
   Size:        210      43     333     196     211     611      93      74

Cuts at:   7508    7698    7760    8490     356 
   Size:        190      62     730    1582

  Fragments arranged by size: 

                1582    1160    1007     931     730     611     453     381
                 333     294     279     211     210     207     201     196
                 190     159     159      93      74      62      62      43
                  41      21      15      11
 
BspEI T'CCGG_A

Cuts at:   2345    4652    5327    8936    2345 
   Size:       2307     675    3609    3125

  Fragments arranged by size: 

                3609    3125    2307     675
 
BspHI T'CATG_A

Cuts at:   8892    8892 
   Size:       9716
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    124    1102    1150    2253    3779    5867    6341    6427    6766 
   Size:        978      48    1103    1526    2088     474      86     339

Cuts at:   6766    6980    7282    7663     124 
   Size:        214     302     381    2177

  Fragments arranged by size: 

                2177    2088    1526    1103     978     474     381     339
                 302     214      86      48
 
BsrBI CCG'CTC

Cuts at:     50    8008    8062    9546    9711      50 
   Size:       7958      54    1484     165      55

  Fragments arranged by size: 

                7958    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    529     789    2315    5573    6074    7628     529 
   Size:        260    1526    3258     501    1554    2617

  Fragments arranged by size: 

                3258    2617    1554    1526     501     260
 
BsrFI r'CCGG_y

Cuts at:    701    1169    1210    1675    2411    5851    7714    7895    8104 
   Size:        468      41     465     736    3440    1863     181     209

Cuts at:   8104     701 
   Size:       2313

  Fragments arranged by size: 

                3440    2313    1863     736     468     465     209     181
                  41
 
BsrGI T'GTAC_A

Cuts at:   2072    2072 
   Size:       9716
 
BssSI C'ACGA_G

Cuts at:   1849    2086    7987    8345    1849 
   Size:        237    5901     358    3220

  Fragments arranged by size: 

                5901    3220     358     237
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1778    6238    7093    1778 
   Size:       4460     855    4401

  Fragments arranged by size: 

                4460    4401     855
 
BstBI TT'CG_AA

Cuts at:    824    8077     824 
   Size:       7253    2463
 
BstEII G'GTnAC_C

Cuts at:   2499    5854    2499 
   Size:       3355    6361
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2092    2514    3060    3447    5502    2092 
   Size:        422     546     387    2055    6306

  Fragments arranged by size: 

                6306    2055     546     422     387
 
BstYI r'GATC_y

Cuts at:      1     289     306    2304    4143    4496    5969    6466    6741 
   Size:        288      17    1998    1839     353    1473     497     275

Cuts at:   6741    7208    7231    7566    7812    8824    8910    8922    9268 
   Size:        467      23     335     246    1012      86      12     346

Cuts at:   9268       1 
   Size:        449

  Fragments arranged by size: 

                1998    1839    1473    1012     497     467     449     353
                 346     335     288     275     246      86      23      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:    514    2389    4054    4303    5992     514 
   Size:       1875    1665     249    1689    4238

  Fragments arranged by size: 

                4238    1875    1689    1665     249
 
BtgI C'CryG_G

Cuts at:    107     978    1350    1716    3813    4037    5234    5366    5687 
   Size:        871     372     366    2097     224    1197     132     321

Cuts at:   5687    6342    6446     107 
   Size:        655     104    3377

  Fragments arranged by size: 

                3377    2097    1197     871     655     372     366     321
                 224     132     104
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    397     950    5430    7666    7847    8010     397 
   Size:        553    4480    2236     181     163    2103

  Fragments arranged by size: 

                4480    2236    2103     553     181     163
 
BtsI GCAGTG_nn'

Cuts at:    724     882    1044    1192    1350    1378    1509    1750    2558 
   Size:        158     162     148     158      28     131     241     808

Cuts at:   2558    5744    6959     724 
   Size:       3186    1215    3481

  Fragments arranged by size: 

                3481    3186    1215     808     241     162     158     158
                 148     131      28
 
ClaI AT'CG_AT

Cuts at:   2597    2654    2597 
   Size:         57    9659
 
DraI TTT'AAA

Cuts at:   6715    6715 
   Size:       9716
 
DraIII CAC_nnn'GTG

Cuts at:   2463    2463 
   Size:       9716
 
DrdI GACnn_nn'nnGTC

Cuts at:   5593    7422    8280    5593 
   Size:       1829     858    7029

  Fragments arranged by size: 

                7029    1829     858
 
EaeI y'GGCC_r

Cuts at:   1260    1347    1353    1539    1719    1812    1833    1875    2231 
   Size:         87       6     186     180      93      21      42     356

Cuts at:   2231    3134    3626    5555    7301    7475    7866    7893    9238 
   Size:        903     492    1929    1746     174     391      27    1345

Cuts at:   9238    1260 
   Size:       1738

  Fragments arranged by size: 

                1929    1746    1738    1345     903     492     391     356
                 186     180     174      93      87      42      27      21
                   6
 
EagI C'GGCC_G

Cuts at:   1539    7301    1539 
   Size:       5762    3954
 
EarI CTCTTCn'nnn_

Cuts at:    230    1225    2009    2200    2488    3992    5725    7739    7949 
   Size:        995     784     191     288    1504    1733    2014     210

Cuts at:   7949     230 
   Size:       1997

  Fragments arranged by size: 

                2014    1997    1733    1504     995     784     288     210
                 191
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   7924    8234    8380    9526    7924 
   Size:        310     146    1146    8114

  Fragments arranged by size: 

                8114    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1044    1323    5735    6321    6728    1044 
   Size:        279    4412     586     407    4032

  Fragments arranged by size: 

                4412    4032     586     407     279
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    331     332     505     514     535     591     635    1325    2173 
   Size:          1     173       9      21      56      44     690     848

Cuts at:   2173    2215    2273    2512    2716    2921    3064    3653    3816 
   Size:         42      58     239     204     205     143     589     163

Cuts at:   3816    4564    4879    5316    6430    6663    7541    7973    8720 
   Size:        748     315     437    1114     233     878     432     747

Cuts at:   8720    8988     331 
   Size:        268    1059

  Fragments arranged by size: 

                1114    1059     878     848     748     747     690     589
                 437     432     315     268     239     233     205     204
                 173     163     143      58      56      44      42      21
                   9       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:    560    5480    5556     560 
   Size:       4920      76    4720

  Fragments arranged by size: 

                4920    4720      76
 
EcoO109I rG'GnC_Cy

Cuts at:    177     581     652    1632    2481    2482    3075    3677    4689 
   Size:        404      71     980     849       1     593     602    1012

Cuts at:   4689    5722    5723    5987    6855     177 
   Size:       1033       1     264     868    3038

  Fragments arranged by size: 

                3038    1033    1012     980     868     849     602     593
                 404     264      71       1       1
 
EcoRI G'AATT_C

Cuts at:   4828    6265    6720    4828 
   Size:       1437     455    7824

  Fragments arranged by size: 

                7824    1437     455
 
EcoRV GAT'ATC

Cuts at:   2450    2450 
   Size:       9716
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   7735    7735 
   Size:       9716
 
FspI TGC'GCA

Cuts at:   7497    7497 
   Size:       9716
 
HaeII r_GCGC'y

Cuts at:   2225    6136    7150    7398    8420    9399    2225 
   Size:       3911    1014     248    1022     979    2542

  Fragments arranged by size: 

                3911    2542    1022    1014     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    298     330     800     832    1640    1672    1904    1936    2116 
   Size:         32     470      32     808      32     232      32     180

Cuts at:   2116    2148    4340    4372    4455    4487    5497    5529    5788 
   Size:         32    2192      32      83      32    1010      32     259

Cuts at:   5788    5820    5905    5937    6073    6105    6142    6174    7558 
   Size:         32      85      32     136      32      37      32    1384

Cuts at:   7558    7588    7590    7620     298 
   Size:         30       2      30    2394

  Fragments arranged by size: 

                2394    2192    1384    1010     808     470     259     232
                 180     136      85      83      37      32      32      32
                  32      32      32      32      32      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   2025    4260    4977    6564    6609    6755    2025 
   Size:       2235     717    1587      45     146    4986

  Fragments arranged by size: 

                4986    2235    1587     717     146      45
 
HindIII A'AGCT_T

Cuts at:    363     363 
   Size:       9716
 
HpaI GTT'AAC

Cuts at:   6609    6609 
   Size:       9716
 
KasI G'GCGC_C

Cuts at:   7146    7394    7146 
   Size:        248    9468
 
KpnI G_GTAC'C

Cuts at:   6736    6736 
   Size:       9716
 
MfeI C'AATT_G

Cuts at:    966    6026     966 
   Size:       5060    4656
 
MluI A'CGCG_T

Cuts at:   9149    9149 
   Size:       9716
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3041    4745    5786    8387    8571    3041 
   Size:       1704    1041    2601     184    4186

  Fragments arranged by size: 

                4186    2601    1704    1041     184
 
MscI TGG'CCA

Cuts at:   1349    1814    1877    2233    3136    5557    7477    1349 
   Size:        465      63     356     903    2421    1920    3588

  Fragments arranged by size: 

                3588    2421    1920     903     465     356      63
 
MslI CAynn'nnrTG

Cuts at:   1256    2090    3427    3700    4246    5449    7705    7832    1256 
   Size:        834    1337     273     546    1203    2256     127    3140

  Fragments arranged by size: 

                3140    2256    1337    1203     834     546     273     127
            
 
MspA1I CmG'CkG

Cuts at:    126     762    1079    1265    1358    1451    1544    1823    1931 
   Size:        636     317     186      93      93      93     279     108

Cuts at:   1931    1955    3397    3815    4155    4216    5032    5294    6827 
   Size:         24    1442     418     340      61     816     262    1533

Cuts at:   6827    7141    7501    8514    8759     126 
   Size:        314     360    1013     245    1083

  Fragments arranged by size: 

                1533    1442    1083    1013     816     636     418     360
                 340     317     314     279     262     245     186     108
                  93      93      93      61      24
 
NaeI GCC'GGC

Cuts at:   7897    7897 
   Size:       9716
 
NarI GG'CG_CC

Cuts at:   7147    7395    7147 
   Size:        248    9468
 
NcoI C'CATG_G

Cuts at:    107    1350    1716    4037    5234    5366    6342    6446     107 
   Size:       1243     366    2321    1197     132     976     104    3377

  Fragments arranged by size: 

                3377    2321    1243    1197     976     366     132     104
            
 
NdeI CA'TA_TG

Cuts at:   6234    6234 
   Size:       9716
 
NgoMIV G'CCGG_C

Cuts at:   7895    7895 
   Size:       9716
 
NspI r_CATG'y

Cuts at:    896    1153    1560    2748    3307    6769    7269    8176     896 
   Size:        257     407    1188     559    3462     500     907    2436

  Fragments arranged by size: 

                3462    2436    1188     907     559     500     407     257
            
 
PasI CC'CwG_GG

Cuts at:   1164    1613    6306    1164 
   Size:        449    4693    4574

  Fragments arranged by size: 

                4693    4574     449
 
PciI A'CATG_T

Cuts at:    892    2744    3303    8172     892 
   Size:       1852     559    4869    2436

  Fragments arranged by size: 

                4869    2436    1852     559
 
PflMI CCAn_nnn'nTGG

Cuts at:    249    3186     249 
   Size:       2937    6779
 
PfoI T'CCnGG_A

Cuts at:   2144    2192    3977    4477    4750    5023    5644    2144 
   Size:         48    1785     500     273     273     621    6216

  Fragments arranged by size: 

                6216    1785     621     500     273     273      48
 
PmeI GTTT'AAAC

Cuts at:   6715    6715 
   Size:       9716
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2665    2697    5376    5408    8855    8887    2665 
   Size:         32    2679      32    3447      32    3494

  Fragments arranged by size: 

                3494    3447    2679      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    177    3075     177 
   Size:       2898    6818
 
PshAI GACnn'nnGTC

Cuts at:   3759    3759 
   Size:       9716
 
PspOMI G'GGCC_C

Cuts at:   2481    5722    5986    2481 
   Size:       3241     264    6211

  Fragments arranged by size: 

                6211    3241     264
 
PspXI vC'TCGA_Gb

Cuts at:   4327    5677    4327 
   Size:       1350    8366
 
PstI C_TGCA'G

Cuts at:    534     757     850     877    1039    1225    1345    1411    1504 
   Size:        223      93      27     162     186     120      66      93

Cuts at:   1504    1624    3099    6763    7448     534 
   Size:        120    1475    3664     685    2802

  Fragments arranged by size: 

                3664    2802    1475     685     223     186     162     120
                 120      93      93      66      27
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       9716
 
PvuII CAG'CTG

Cuts at:    126     762    3397    4155    4216    5032    5294    7141    7501 
   Size:        636    2635     758      61     816     262    1847     360

Cuts at:   7501     126 
   Size:       2341

  Fragments arranged by size: 

                2635    2341    1847     816     758     636     360     262
                  61
 
RsrII CG'GwC_CG

Cuts at:   7911    7911 
   Size:       9716
 
SacI G_AGCT'C

Cuts at:   1046    1325    5737    6323    6730    1046 
   Size:        279    4412     586     407    4032

  Fragments arranged by size: 

                4412    4032     586     407     279
 
SacII CC_GC'GG

Cuts at:   3816    3816 
   Size:       9716
 
SalI G'TCGA_C

Cuts at:   6753    6753 
   Size:       9716
 
SapI GCTCTTCn'nnn_

Cuts at:   2200    5725    7739    7949    2200 
   Size:       3525    2014     210    3967

  Fragments arranged by size: 

                3967    3525    2014     210
 
SbfI CC_TGCA'GG

Cuts at:   6763    6763 
   Size:       9716
 
ScaI AGT'ACT

Cuts at:   2170    2680    3688    4391    4611    9233    2170 
   Size:        510    1008     703     220    4622    2653

  Fragments arranged by size: 

                4622    2653    1008     703     510     220
 
SexAI A'CCwGG_T

Cuts at:    500    2572    3989     500 
   Size:       2072    1417    6227

  Fragments arranged by size: 

                6227    2072    1417
 
SfcI C'TryA_G

Cuts at:     33     530     588     753     846     873    1035    1221    1341 
   Size:        497      58     165      93      27     162     186     120

Cuts at:   1341    1407    1500    1620    1950    3095    3558    4124    4377 
   Size:         66      93     120     330    1145     463     566     253

Cuts at:   4377    5219    5925    6759    7444    8437    8628      33 
   Size:        842     706     834     685     993     191    1121

  Fragments arranged by size: 

                1145    1121     993     842     834     706     685     566
                 497     463     330     253     191     186     165     162
                 120     120      93      93      66      58      27
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   5516    5516 
   Size:       9716
 
SfoI GGC'GCC

Cuts at:   7148    7396    7148 
   Size:        248    9468
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9716
 
SmaI CCC'GGG

Cuts at:   2479    4322    5839    6738    2479 
   Size:       1843    1517     899    5457

  Fragments arranged by size: 

                5457    1843    1517     899
 
SmlI C'TyrA_G

Cuts at:    181    1324    3508    4327    4938    5677    6871    7786    8278 
   Size:       1143    2184     819     611     739    1194     915     492

Cuts at:   8278    8540     181 
   Size:        262    1357

  Fragments arranged by size: 

                2184    1357    1194    1143     915     819     739     611
                 492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       9716
 
SphI G_CATG'C

Cuts at:   6769    7269    6769 
   Size:        500    9216
 
StuI AGG'CCT

Cuts at:   1865    2258    2783    3233    3406    3586    4052    4405    4871 
   Size:        393     525     450     173     180     466     353     466

Cuts at:   4871    1865 
   Size:       6710

  Fragments arranged by size: 

                6710     525     466     466     450     393     353     180
                 173
 
StyI C'CwwG_G

Cuts at:    107    1350    1716    4037    4917    5234    5366    5552    6050 
   Size:       1243     366    2321     880     317     132     186     498

Cuts at:   6050    6342    6446    6850    7204     107 
   Size:        292     104     404     354    2619

  Fragments arranged by size: 

                2619    2321    1243     880     498     404     366     354
                 317     292     186     132     104
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1177    2098    3174    3916    4930    5319    5686    5968    7299 
   Size:        921    1076     742    1014     389     367     282    1331

Cuts at:   7299    7432    7891    8100    1177 
   Size:        133     459     209    2793

  Fragments arranged by size: 

                2793    1331    1076    1014     921     742     459     389
                 367     282     209     133
 
TatI w'GTAC_w

Cuts at:    526    2072    2168    2678    3251    3686    4389    4609    9231 
   Size:       1546      96     510     573     435     703     220    4622

Cuts at:   9231     526 
   Size:       1011

  Fragments arranged by size: 

                4622    1546    1011     703     573     510     435     220
                  96
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    974     985    1006    1017    1171    1203    1312    1344    1636 
   Size:         11      21      11     154      32     109      32     292

Cuts at:   1636    1668    1822    1854    2284    2316    3605    3637    6164 
   Size:         32     154      32     430      32    1289      32    2527

Cuts at:   6164    6196    6423    6455    9233    9265     974 
   Size:         32     227      32    2778      32    1425

  Fragments arranged by size: 

                2778    2527    1425    1289     430     292     227     154
                 154     109      32      32      32      32      32      32
                  32      32      32      21      11      11
 
Tth111I GACn'n_nGTC

Cuts at:   7513    7513 
   Size:       9716
 
XbaI T'CTAG_A

Cuts at:   2310    6747    2310 
   Size:       4437    5279
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    567    2511    4539    5313    5373    5580    5980    7211     567 
   Size:       1944    2028     774      60     207     400    1231    3072

  Fragments arranged by size: 

                3072    2028    1944    1231     774     400     207      60
            
 
XhoI C'TCGA_G

Cuts at:   4327    5677    4327 
   Size:       1350    8366
 
XmaI C'CCGG_G

Cuts at:   2477    4320    5837    6736    2477 
   Size:       1843    1517     899    5457

  Fragments arranged by size: 

                5457    1843    1517     899
 
XmnI GAAnn'nnTTC

Cuts at:    365     365 
   Size:       9716
 
ZraI GAC'GTC

Cuts at:   2132    2132 
   Size:       9716
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AscI    AvrII    BfrBI     BmtI    BsiWI   BssHII
  BstZ17I    CspCI     FseI    FspAI     NheI     NotI     NruI     NsiI
     PacI     PmlI     PsiI     PsrI    SanDI    SgrAI    SnaBI     SrfI
     SspI     SwaI
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