Restriction map for pF1KB0045
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# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       AcyI        AflII       AgeI        AhdI        
Aor51HI     ApaI        AscI        AseI        AsnI        
AspEI       AviII       AvrII       BbeI        BbrPI       
BcgI        BciVI       BfrI        BglII       BlnI        
BsaAI       BsaHI       BsaI        BsiEI       BsiWI       
Bsp1407I    BspDI       BspHI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstEII      
BstPI       BstZ17I     CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO109I    EcoO65I     FauI        FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HindIII     
KasI        KspI        MluI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
SwaI        XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       2218  GGTACC               G^GTACC
AccI         1874  GTCTAC               GT^[AC][GT]AC
AccII          53  CGCG                 CG^CG
AccIII         47  TCCGGA               T^CCGGA
AclI          638  AACGTT               AA^CGTT
Alw44I        890  GTGCAC               G^TGCAC
AocI         2222  CCTTAGG              CC^T[ACGT]AGG
ApaLI         890  GTGCAC               G^TGCAC
Asp700I       710  GAACACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2218  GGTACC               G^GTACC
BalI          232  TGGCCA               TGG^CCA
BamHI        3775  GGATCC               G^GATCC
BclI         3417  TGATCA               T^GATCA
BcnI         1240  CCCGG                CC^[GC]GG
BglI          153  GCCAGGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI          47  TCCGGA               [AT]^CCGG[AT]
BseAI          47  TCCGGA               T^CCGGA
BspEI          47  TCCGGA               T^CCGGA
BsrDI         608  CATTGC               GCAATG(2/0)
BstBI        2556  TTCGAA               TT^CGAA
BstUI          53  CGCG                 CG^CG
Bsu36I       2222  CCTTAGG              CC^T[ACGT]AGG
EaeI          232  TGGCCA               [CT]^GGCC[GA]
Eco81I       2222  CCTTAGG              CC^T[ACGT]AGG
EcoRV        1036  GATATC               GAT^ATC
EcoT14I      3021  CCTTGG               C^C[AT][AT]GG
EcoT22I      1905  ATGCAT               ATGCA^T
FbaI         3417  TGATCA               T^GATCA
HpaI          375  GTTAAC               GTT^AAC
KpnI         2218  GGTACC               GGTAC^C
MfeI          470  CAATTG               C^AATTG
MluNI         232  TGGCCA               TGG^CCA
MroI           47  TCCGGA               T^CCGGA
MscI          232  TGGCCA               TGG^CCA
MunI          470  CAATTG               C^AATTG
MvnI           53  CGCG                 CG^CG
NciI         1240  CCCGG                CC^[GC]GG
NsiI         1905  ATGCAT               ATGCA^T
NspV         2556  TTCGAA               TT^CGAA
Psp1406I      638  AACGTT               AA^CGTT
SapI         3247  GAAGAGC              GCTCTTC(1/4)
SfuI         2556  TTCGAA               TT^CGAA
StuI          382  AGGCCT               AGG^CCT
StyI         3021  CCTTGG               C^C[AT][AT]GG
XcmI         3530  CCAATCCACCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          710  GAACACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII       1870  ACATGT               A^C[GA][CT]GT
             3064  ACATGT              
AspI         3857  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3963  GACAGTGTC           
BbvCI        3803  CCTCAGC              CCTCAGC(-5/-2)
             4151  CCTCAGC             
BlpI          877  GCTAAGC              GC^T[ACGT]AGC
             1753  GCTCAGC             
Bpu1102I      877  GCTAAGC              GC^T[ACGT]AGC
             1753  GCTCAGC             
BsaBI        1032  GATAGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3899  GATCTATATC          
BsmBI        1511  CGTCTC               CGTCTC(1/5)
             1347  GAGACG              
BspLU11I     1870  ACATGT               A^CATGT
             3064  ACATGT              
BstXI         613  CCACCTCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2480  CCAAATGTTTGG        
CelII         877  GCTAAGC              GC^T[ACGT]AGC
             1753  GCTCAGC             
EcoNI         492  CCTTGTAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2386  CCTTCTGGAGG         
HgaI           51  GACGC                GACGC(5/10)
             4289  GACGC               
HincII        375  GTTAAC               GT[CT]^[GA]AC
             3260  GTCAAC              
HindII        375  GTTAAC               GT[CT]^[GA]AC
             3260  GTCAAC              
MaeII         639  ACGT                 A^CGT
             3131  ACGT                
MamI         1032  GATAGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3899  GATCTATATC          
PflFI        3857  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3963  GACAGTGTC           
SacI          276  GAGCTC               GAGCT^C
             3395  GAGCTC              
SmlI         1514  CTCAAG               C^T[CT][GA]AG
             3638  CTCAAG              
SpeI          346  ACTAGT               A^CTAGT
             3403  ACTAGT              
TaqI         1134  TCGA                 T^CGA
             2557  TCGA                
Tth111I      3857  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3963  GACAGTGTC           
TthHB8I      1134  TCGA                 T^CGA
             2557  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EarI        EcoRI       EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SspI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Van91I      XhoII       
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