Restriction map for pF1KB0009
 (Circular) (Six-base) MAPSORT of: pF1KB0009.seq  Check: 3622  from: 1  to: 8075



pF1KB0009  8075 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    713     998    1485    1737     713 
   Size:        285     487     252    7051

  Fragments arranged by size: 

                7051     487     285     252
 
AccI GT'mk_AC

Cuts at:   5113    5113 
   Size:       8075
 
Acc65I G'GTAC_C

Cuts at:   5091    5091 
   Size:       8075
 
AclI AA'CG_TT

Cuts at:   7915    7915 
   Size:       8075
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    841    2071    2819    3079    4375    4607    4846    5900    6332 
   Size:       1230     748     260    1296     232     239    1054     432

Cuts at:   6332    7079    7347     841 
   Size:        747     268    1569

  Fragments arranged by size: 

                1569    1296    1230    1054     748     747     432     268
                 260     239     232
 
AfeI AGC'GCT

Cuts at:   3495    3495 
   Size:       8075
 
AflII C'TTAA_G

Cuts at:   3268    3268 
   Size:       8075
 
AflIII A'CryG_T

Cuts at:    371    2030    5427    6531    7508     371 
   Size:       1659    3397    1104     977     938

  Fragments arranged by size: 

                3397    1659    1104     977     938
 
AgeI A'CCGG_T

Cuts at:   6463    6463 
   Size:       8075
 
AhdI GACnn_n'nnGTC

Cuts at:   4539    4539 
   Size:       8075
 
AleI CACnn'nnGTG

Cuts at:   2942    2942 
   Size:       8075
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2960    2960 
   Size:       8075
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1567    1599    2362    2394    1567 
   Size:         32     763      32    7248

  Fragments arranged by size: 

                7248     763      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    861     936     990    1389    1629    1713    2122    2142    2538 
   Size:         75      54     399     240      84     409      20     396

Cuts at:   2538    2610    2814    3588    5380    6947     861 
   Size:         72     204     774    1792    1567    1989

  Fragments arranged by size: 

                1989    1792    1567     774     409     399     396     240
                 204      84      75      72      54      20
 
ApaI G_GGCC'C

Cuts at:   1833    1833 
   Size:       8075
 
ApaLI G'TGCA_C

Cuts at:    700    1618    6845     700 
   Size:        918    5227    1930

  Fragments arranged by size: 

                5227    1930     918
 
ApoI r'AATT_y

Cuts at:    544    2704    3532    3607    3928    4183    4708    5079    7428 
   Size:       2160     828      75     321     255     525     371    2349

Cuts at:   7428    7684     544 
   Size:        256     935

  Fragments arranged by size: 

                2349    2160     935     828     525     371     321     256
                 255      75
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       8075
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8075
 
AvaI C'yCGr_G

Cuts at:    718     912    1826    1942    2251    3362    3370    5095     718 
   Size:        194     914     116     309    1111       8    1725    3698

  Fragments arranged by size: 

                3698    1725    1111     914     309     194     116       8
            
 
AvrII C'CTAG_G

Cuts at:   3792    3792 
   Size:       8075
 
BamHI G'GATC_C

Cuts at:   2822    3515    4043    5100    2822 
   Size:        693     528    1057    5797

  Fragments arranged by size: 

                5797    1057     693     528
 
BanI G'GyrC_C

Cuts at:    939    3615    4252    5033    5091    5505    5753    5788     939 
   Size:       2676     637     781      58     414     248      35    3226

  Fragments arranged by size: 

                3226    2676     781     637     414     248      58      35
            
 
BanII G_rGCy'C

Cuts at:    415     775    1570    1827    1833    2261    2738    3404    4397 
   Size:        360     795     257       6     428     477     666     993

Cuts at:   4397    5089    6119     415 
   Size:        692    1030    2371

  Fragments arranged by size: 

                2371    1030     993     795     692     666     477     428
                 360     257       6
 
BbeI G_GCGC'C

Cuts at:   5509    5757    5509 
   Size:        248    7827
 
BbsI GAAGACnn'nnnn_

Cuts at:    329     529    1719    2087    2219    2483    2938    3074     329 
   Size:        200    1190     368     132     264     455     136    5330

  Fragments arranged by size: 

                5330    1190     455     368     264     200     136     132
            
 
BbvCI CC'TCA_GC

Cuts at:   3299    3299 
   Size:       8075
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1042    1076    1329    1363    2986    3020    3833    3867    1042 
   Size:         34     253      34    1623      34     813      34    5250

  Fragments arranged by size: 

                5250    1623     813     253      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   5973    6734    8075    5973 
   Size:        761    1341    5973

  Fragments arranged by size: 

                5973    1341     761
 
BclI T'GATC_A

Cuts at:   2144    5595    2144 
   Size:       3451    4624
 
BfrBI ATG'CAT

Cuts at:   4452    4452 
   Size:       8075
 
BglI GCCn_nnn'nGGC

Cuts at:   1529    3097    1529 
   Size:       1568    6507
 
BglII A'GATC_T

Cuts at:   1280    1949    4085    4586    5590    7627    1280 
   Size:        669    2136     501    1004    2037    1728

  Fragments arranged by size: 

                2136    2037    1728    1004     669     501
 
BlpI GC'TnA_GC

Cuts at:   1379    3497    5187    1379 
   Size:       2118    1690    4267

  Fragments arranged by size: 

                4267    2118    1690
 
Bme1580I G_kGCm'C

Cuts at:    704     944    1622    1833    2949    4255    5700    5793    6849 
   Size:        240     678     211    1116    1306    1445      93    1056

Cuts at:   6849     704 
   Size:       1930

  Fragments arranged by size: 

                1930    1445    1306    1116    1056     678     240     211
                  93
 
BmgBI CAC'GTC

Cuts at:    372    1635     372 
   Size:       1263    6812
 
BmrI ACTGGGnnnn_n'

Cuts at:   3338    5287    5368    5703    7847    3338 
   Size:       1949      81     335    2144    3566

  Fragments arranged by size: 

                3566    2144    1949     335      81
 
BmtI G_CTAG'C

Cuts at:    742    2566     742 
   Size:       1824    6251
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6442    6442 
   Size:       8075
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    697     712    1093    1141    1690    1820    1993    2776    2965 
   Size:         15     381      48     549     130     173     783     189

Cuts at:   2965    3157    3581    3802    3844    3934    4144    4261    4429 
   Size:        192     424     221      42      90     210     117     168

Cuts at:   4429    4495    5066     697 
   Size:         66     571    3706

  Fragments arranged by size: 

                3706     783     571     549     424     381     221     210
                 192     189     173     168     130     117      90      66
                  48      42      15
 
Bpu10I CC'TnA_GC

Cuts at:   1564    2230    3299    1564 
   Size:        666    1069    6340

  Fragments arranged by size: 

                6340    1069     666
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    790    1003    3295    4381    5251    6130    6622    6920     790 
   Size:        213    2292    1086     870     879     492     298    1945

  Fragments arranged by size: 

                2292    1945    1086     879     870     492     298     213
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   3559    3988    7779    3559 
   Size:        429    3791    3855

  Fragments arranged by size: 

                3855    3791     429
 
BsaAI yAC'GTr

Cuts at:   1877    4689    5428    1877 
   Size:       2812     739    4524

  Fragments arranged by size: 

                4524    2812     739
 
BsaBI GATnn'nnATC

Cuts at:      6    5589    7626       6 
   Size:       5583    2037     455

  Fragments arranged by size: 

                5583    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2465    3878    5506    5754    6456    7952    2465 
   Size:       1413    1628     248     702    1496    2588

  Fragments arranged by size: 

                2588    1628    1496    1413     702     248
 
BsaWI w'CCGG_w

Cuts at:   3158    4550    5264    5785    6463    6737    6884    7295    3158 
   Size:       1392     714     521     678     274     147     411    3938

  Fragments arranged by size: 

                3938    1392     714     678     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2296    2326    2362    2392    2774    2804    4850    4880    4948 
   Size:         30      36      30     382      30    2046      30      68

Cuts at:   4948    4978    2296 
   Size:         30    5393

  Fragments arranged by size: 

                5393    2046     382      68      36      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    430     631     664     694     850    1960    2386    3589    4042 
   Size:        201      33      30     156    1110     426    1203     453

Cuts at:   4042     430 
   Size:       4463

  Fragments arranged by size: 

                4463    1203    1110     453     426     201     156      33
                  30
 
BseYI C'CCAG_C

Cuts at:    605    1289    2260    3195    3840    4671    5500    6258    6835 
   Size:        684     971     935     645     831     829     758     577

Cuts at:   6835    7331     605 
   Size:        496    1349

  Fragments arranged by size: 

                1349     971     935     831     829     758     684     645
                 577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    955     976    1440    2135    2383    2596    3261    4513     955 
   Size:         21     464     695     248     213     665    1252    4517

  Fragments arranged by size: 

                4517    1252     695     665     464     248     213      21
            
 
BsiEI CG_ry'CG

Cuts at:    104    5617    5663    6871     104 
   Size:       5513      46    1208    1308

  Fragments arranged by size: 

                5513    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    704    1622    2738    2906    3404    4397    4688    5089    5867 
   Size:        918    1116     168     498     993     291     401     778

Cuts at:   5867    6057    6849     704 
   Size:        190     792    1930

  Fragments arranged by size: 

                1930    1116     993     918     792     778     498     401
                 291     190     168
 
BsmI GAATG_Cn'

Cuts at:   1200    3300    1200 
   Size:       2100    5975
 
BsmBI CGTCTCn'nnnn_

Cuts at:    550     550 
   Size:       8075
 
Bsp1286I G_dGCh'C

Cuts at:    415     704     775     944    1570    1622    1827    1833    2261 
   Size:        289      71     169     626      52     205       6     428

Cuts at:   2261    2738    2906    2949    3404    4255    4397    4688    5089 
   Size:        477     168      43     455     851     142     291     401

Cuts at:   5089    5700    5793    5867    6057    6119    6849     415 
   Size:        611      93      74     190      62     730    1641

  Fragments arranged by size: 

                1641     851     730     626     611     477     455     428
                 401     291     289     205     190     169     168     142
                  93      74      71      62      52      43       6
 
BspEI T'CCGG_A

Cuts at:   4550    7295    4550 
   Size:       2745    5330
 
BspHI T'CATG_A

Cuts at:    303    7251     303 
   Size:       6948    1127
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    335     713     998    1485    1737    2943    5125    5339    5641 
   Size:        378     285     487     252    1206    2182     214     302

Cuts at:   5641    6022     335 
   Size:        381    2388

  Fragments arranged by size: 

                2388    2182    1206     487     381     378     302     285
                 252     214
 
BsrBI CCG'CTC

Cuts at:     50    2388    4566    6367    6421    7905    8070      50 
   Size:       2338    2178    1801      54    1484     165      55

  Fragments arranged by size: 

                2338    2178    1801    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2835    4967    5987    2835 
   Size:       2132    1020    4923

  Fragments arranged by size: 

                4923    2132    1020
 
BsrFI r'CCGG_y

Cuts at:   1249    2986    3071    6073    6254    6463    1249 
   Size:       1737      85    3002     181     209    2861

  Fragments arranged by size: 

                3002    2861    1737     209     181      85
 
BsrGI T'GTAC_A

Cuts at:   1740    1740 
   Size:       8075
 
BssSI C'ACGA_G

Cuts at:    337    2383    2905    6346    6704     337 
   Size:       2046     522    3441     358    1708

  Fragments arranged by size: 

                3441    2046    1708     522     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    861     878     990    1418    1626    3054    5452     861 
   Size:         17     112     428     208    1428    2398    3484

  Fragments arranged by size: 

                3484    2398    1428     428     208     112      17
 
BstBI TT'CG_AA

Cuts at:   6436    6436 
   Size:       8075
 
BstEII G'GTnAC_C

Cuts at:   1497    3717    1497 
   Size:       2220    5855
 
BstXI CCAn_nnnn'nTGG

Cuts at:    960    1293    4023    4939     960 
   Size:        333    2730     916    4096

  Fragments arranged by size: 

                4096    2730     916     333
 
BstYI r'GATC_y

Cuts at:      1     473    1280    1949    2607    2822    3515    4031    4043 
   Size:        472     807     669     658     215     693     516      12

Cuts at:   4043    4085    4586    5049    5100    5567    5590    5925    6171 
   Size:         42     501     463      51     467      23     335     246

Cuts at:   6171    7183    7269    7281    7627       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     807     693     669     658     516     501     472
                 467     463     449     346     335     246     215      86
                  51      42      23      12      12
 
Bsu36I CC'TnA_GG

Cuts at:   3754    4145    3754 
   Size:        391    7684
 
BtgI C'CryG_G

Cuts at:   1226    1770    3698    3722    1226 
   Size:        544    1928      24    5579

  Fragments arranged by size: 

                5579    1928     544      24
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   6025    6206    6369    6025 
   Size:        181     163    7731

  Fragments arranged by size: 

                7731     181     163
 
BtsI GCAGTG_nn'

Cuts at:    874     924    1428    1622    2776    4158    5318     874 
   Size:         50     504     194    1154    1382    1160    3631

  Fragments arranged by size: 

                3631    1382    1160    1154     504     194      50
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    575     610    5049    5084     575 
   Size:         35    4439      35    3566

  Fragments arranged by size: 

                4439    3566      35      35
 
DraI TTT'AAA

Cuts at:   4850    5074    4850 
   Size:        224    7851
 
DraIII CAC_nnn'GTG

Cuts at:   1626    1626 
   Size:       8075
 
DrdI GACnn_nn'nnGTC

Cuts at:   5781    6639    5781 
   Size:        858    7217
 
EaeI y'GGCC_r

Cuts at:    350    1592    2921    2988    3662    4289    5660    5834    6225 
   Size:       1242    1329      67     674     627    1371     174     391

Cuts at:   6225    6252    7597     350 
   Size:         27    1345     828

  Fragments arranged by size: 

                1371    1345    1329    1242     828     674     627     391
                 174      67      27
 
EagI C'GGCC_G

Cuts at:   5660    5660 
   Size:       8075
 
EarI CTCTTCn'nnn_

Cuts at:   1054    1303    1834    1853    3893    4308    6098    6308    1054 
   Size:        249     531      19    2040     415    1790     210    2821

  Fragments arranged by size: 

                2821    2040    1790     531     415     249     210      19
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    284    3306    6283    6593    6739    7885     284 
   Size:       3022    2977     310     146    1146     474

  Fragments arranged by size: 

                3022    2977    1146     474     310     146
 
EcoICRI GAG'CTC

Cuts at:   2736    3402    4395    5087    2736 
   Size:        666     993     692    5724

  Fragments arranged by size: 

                5724     993     692     666
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    697     712     841    1093    1141    1690    1820    1993    2071 
   Size:         15     129     252      48     549     130     173      78

Cuts at:   2071    2776    2819    2965    3079    3157    3581    3802    3844 
   Size:        705      43     146     114      78     424     221      42

Cuts at:   3844    3934    4144    4261    4375    4429    4495    4607    4846 
   Size:         90     210     117     114      54      66     112     239

Cuts at:   4846    5066    5900    6332    7079    7347     697 
   Size:        220     834     432     747     268    1425

  Fragments arranged by size: 

                1425     834     747     705     549     432     424     268
                 252     239     221     220     210     173     146     130
                 129     117     114     114     112      90      78      78
                  66      54      48      43      42      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1243    2089    1243 
   Size:        846    7229
 
EcoO109I rG'GnC_Cy

Cuts at:    423     579     723     825    1830    2514    3367    3819    4533 
   Size:        156     144     102    1005     684     853     452     714

Cuts at:   4533    4979    5041    5214     423 
   Size:        446      62     173    3284

  Fragments arranged by size: 

                3284    1005     853     714     684     452     446     173
                 156     144     102      62
 
EcoRI G'AATT_C

Cuts at:   3607    5079    3607 
   Size:       1472    6603
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    255     546    1558    2500    3279    6094     255 
   Size:        291    1012     942     779    2815    2236

  Fragments arranged by size: 

                2815    2236    1012     942     779     291
 
FseI GG_CCGG'CC

Cuts at:   2990    2990 
   Size:       8075
 
FspI TGC'GCA

Cuts at:   1012    1981    1999    2422    5856    1012 
   Size:        969      18     423    3434    3231

  Fragments arranged by size: 

                3434    3231     969     423      18
 
FspAI rTGC'GCAy

Cuts at:   1981    1981 
   Size:       8075
 
HaeII r_GCGC'y

Cuts at:    698    2242    2547    3497    3508    5509    5757    6779    7758 
   Size:       1544     305     950      11    2001     248    1022     979

Cuts at:   7758     698 
   Size:       1015

  Fragments arranged by size: 

                2001    1544    1022    1015     979     950     305     248
                  11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    160     192     877     909     939     971    2160    2192    3402 
   Size:         32     685      32      30      32    1189      32    1210

Cuts at:   3402    3434    4525    4557    4948    4980    5041    5073    5917 
   Size:         32    1091      32     391      32      61      32     844

Cuts at:   5917    5947    5949    5979     160 
   Size:         30       2      30    2256

  Fragments arranged by size: 

                2256    1210    1189    1091     844     685     391      61
                  32      32      32      32      32      32      32      32
                  30      30      30       2
 
HincII GTy'rAC

Cuts at:   1206    3801    5114    1206 
   Size:       2595    1313    4167

  Fragments arranged by size: 

                4167    2595    1313
 
HindIII A'AGCT_T

Cuts at:    200    2017    3646     200 
   Size:       1817    1629    4629

  Fragments arranged by size: 

                4629    1817    1629
 
KasI G'GCGC_C

Cuts at:   5505    5753    5505 
   Size:        248    7827
 
KpnI G_GTAC'C

Cuts at:   5095    5095 
   Size:       8075
 
MluI A'CGCG_T

Cuts at:   7508    7508 
   Size:       8075
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1733    2081    2312    2858    2940    4250    4793    6746    6930 
   Size:        348     231     546      82    1310     543    1953     184

Cuts at:   6930    1733 
   Size:       2878

  Fragments arranged by size: 

                2878    1953    1310     546     543     348     231     184
                  82
 
MscI TGG'CCA

Cuts at:    352    2923    4291    5836     352 
   Size:       2571    1368    1545    2591

  Fragments arranged by size: 

                2591    2571    1545    1368
 
MslI CAynn'nnrTG

Cuts at:    403    1225    2446    2942    2996    3692    6064    6191     403 
   Size:        822    1221     496      54     696    2372     127    2287

  Fragments arranged by size: 

                2372    2287    1221     822     696     496     127      54
            
 
MspA1I CmG'CkG

Cuts at:    469     609     858     878    1041    1293    1557    1710    2013 
   Size:        140     249      20     163     252     264     153     303

Cuts at:   2013    2535    2892    2898    3660    4278    5186    5500    5860 
   Size:        522     357       6     762     618     908     314     360

Cuts at:   5860    6873    7118     469 
   Size:       1013     245    1426

  Fragments arranged by size: 

                1426    1013     908     762     618     522     360     357
                 314     303     264     252     249     245     163     153
                 140      20       6
 
NaeI GCC'GGC

Cuts at:   2988    6256    2988 
   Size:       3268    4807
 
NarI GG'CG_CC

Cuts at:   5506    5754    5506 
   Size:        248    7827
 
NcoI C'CATG_G

Cuts at:   1770    1770 
   Size:       8075
 
NgoMIV G'CCGG_C

Cuts at:   2986    6254    2986 
   Size:       3268    4807
 
NheI G'CTAG_C

Cuts at:    738    2562     738 
   Size:       1824    6251
 
NsiI A_TGCA'T

Cuts at:   4454    4454 
   Size:       8075
 
NspI r_CATG'y

Cuts at:    408    1009    2034    3342    4249    4456    4464    5128    5628 
   Size:        601    1025    1308     907     207       8     664     500

Cuts at:   5628    6535     408 
   Size:        907    1948

  Fragments arranged by size: 

                1948    1308    1025     907     907     664     601     500
                 207       8
 
PasI CC'CwG_GG

Cuts at:   1570    4039    1570 
   Size:       2469    5606
 
PciI A'CATG_T

Cuts at:   2030    6531    2030 
   Size:       4501    3574
 
PflMI CCAn_nnn'nTGG

Cuts at:    936    1629     936 
   Size:        693    7382
 
PfoI T'CCnGG_A

Cuts at:    728     959    2024    2135    3011    3134    4238    4511     728 
   Size:        231    1065     111     876     123    1104     273    4292

  Fragments arranged by size: 

                4292    1104    1065     876     273     231     123     111
            
 
PmeI GTTT'AAAC

Cuts at:   5074    5074 
   Size:       8075
 
PmlI CAC'GTG

Cuts at:   4689    4689 
   Size:       8075
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    653     685    2362    2394    7214    7246     653 
   Size:         32    1677      32    4820      32    1482

  Fragments arranged by size: 

                4820    1677    1482      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    423     723    2514    3367    3819    4533    4979    5041     423 
   Size:        300    1791     853     452     714     446      62    3457

  Fragments arranged by size: 

                3457    1791     853     714     452     446     300      62
            
 
PspOMI G'GGCC_C

Cuts at:   1829    1829 
   Size:       8075
 
PspXI vC'TCGA_Gb

Cuts at:    718     718 
   Size:       8075
 
PstI C_TGCA'G

Cuts at:    455     869     883    1556    1718    1838    2342    2543    2897 
   Size:        414      14     673     162     120     504     201     354

Cuts at:   2897    2954    3410    5122    5807     455 
   Size:         57     456    1712     685    2723

  Fragments arranged by size: 

                2723    1712     685     673     504     456     414     354
                 201     162     120      57      14
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       8075
 
PvuII CAG'CTG

Cuts at:    469     609    1041    1293    2892    3660    5500    5860     469 
   Size:        140     432     252    1599     768    1840     360    2684

  Fragments arranged by size: 

                2684    1840    1599     768     432     360     252     140
            
 
RsrII CG'GwC_CG

Cuts at:    114    6270     114 
   Size:       6156    1919
 
SacI G_AGCT'C

Cuts at:   2738    3404    4397    5089    2738 
   Size:        666     993     692    5724

  Fragments arranged by size: 

                5724     993     692     666
 
SalI G'TCGA_C

Cuts at:   5112    5112 
   Size:       8075
 
SanDI GG'GwC_CC

Cuts at:   3367    4533    3367 
   Size:       1166    6909
 
SapI GCTCTTCn'nnn_

Cuts at:   1054    1303    6098    6308    1054 
   Size:        249    4795     210    2821

  Fragments arranged by size: 

                4795    2821     249     210
 
SbfI CC_TGCA'GG

Cuts at:   2954    5122    2954 
   Size:       2168    5907
 
ScaI AGT'ACT

Cuts at:   7592    7592 
   Size:       8075
 
SexAI A'CCwGG_T

Cuts at:   1622    1622 
   Size:       8075
 
SfcI C'TryA_G

Cuts at:     33     451     865     879    1552    1714    1834    2338    2539 
   Size:        418     414      14     673     162     120     504     201

Cuts at:   2539    2893    2950    3406    3738    5118    5803    6796    6987 
   Size:        354      57     456     332    1380     685     993     191

Cuts at:   6987      33 
   Size:       1121

  Fragments arranged by size: 

                1380    1121     993     685     673     504     456     418
                 414     354     332     201     191     162     120      57
                  14
 
SfoI GGC'GCC

Cuts at:   5507    5755    5507 
   Size:        248    7827
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8075
 
SmaI CCC'GGG

Cuts at:    914    1828    5097     914 
   Size:        914    3269    3892

  Fragments arranged by size: 

                3892    3269     914
 
SmlI C'TyrA_G

Cuts at:    718     805    1018    3268    3310    4396    5230    6145    6637 
   Size:         87     213    2250      42    1086     834     915     492

Cuts at:   6637    6899     718 
   Size:        262    1894

  Fragments arranged by size: 

                2250    1894    1086     915     834     492     262     213
                  87      42
 
SnaBI TAC'GTA

Cuts at:   1877    1877 
   Size:       8075
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       8075
 
SphI G_CATG'C

Cuts at:   3342    4249    4464    5128    5628    3342 
   Size:        907     215     664     500    5789

  Fragments arranged by size: 

                5789     907     664     500     215
 
SrfI GCCC'GGGC

Cuts at:   1828    1828 
   Size:       8075
 
StuI AGG'CCT

Cuts at:   1780    3262    3274    1780 
   Size:       1482      12    6581

  Fragments arranged by size: 

                6581    1482      12
 
StyI C'CwwG_G

Cuts at:   1610    1770    3792    4592    5209    5563    1610 
   Size:        160    2022     800     617     354    4122

  Fragments arranged by size: 

                4122    2022     800     617     354     160
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1303    2322    3705    4663    4696    5658    5791    6250    6459 
   Size:       1019    1383     958      33     962     133     459     209

Cuts at:   6459    1303 
   Size:       2919

  Fragments arranged by size: 

                2919    1383    1019     962     958     459     209     133
                  33
 
TatI w'GTAC_w

Cuts at:   1740    2435    2618    3314    7590    1740 
   Size:        695     183     696    4276    2225

  Fragments arranged by size: 

                4276    2225     696     695     183
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1288    1320    1393    1425    2296    2328    4850    4882    7592 
   Size:         32      73      32     871      32    2522      32    2710

Cuts at:   7592    7624    1288 
   Size:         32    1739

  Fragments arranged by size: 

                2710    2522    1739     871      73      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3220    5872    3220 
   Size:       2652    5423
 
XbaI T'CTAG_A

Cuts at:   2178    5106    2178 
   Size:       2928    5147
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   5570    5570 
   Size:       8075
 
XhoI C'TCGA_G

Cuts at:    718     718 
   Size:       8075
 
XmaI C'CCGG_G

Cuts at:    912    1826    5095     912 
   Size:        914    3269    3892

  Fragments arranged by size: 

                3892    3269     914
 
XmnI GAAnn'nnTTC

Cuts at:    461    2084    2500    4814     461 
   Size:       1623     416    2314    3722

  Fragments arranged by size: 

                3722    2314    1623     416
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AscI     BaeI    BsiWI   BssHII  BstZ17I     ClaI    EcoRV
     HpaI     MfeI     NdeI     NotI     NruI     PacI    PshAI     PsiI
     PsrI    SacII     SfiI    SgrAI     SspI     SwaI     ZraI
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