Restriction map for pF1KA2010
 (Linear) (Six-base) MAPSORT of: KIAA2010.seq  Check: 8047  from: 1  to: 2460



KIAA2010.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AatII G_ACGT'C

Cuts at:      0     659    2460 
   Size:        659    1801
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     239     371     659    2460 
   Size:        124     115     132     288    1801

  Fragments arranged by size: 

                1801     288     132     124     115
 
AflIII A'CryG_T

Cuts at:      0     851    2460 
   Size:        851    1609
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     133    2460 
   Size:        133    2327
 
AlwNI CAG_nnn'CTG

Cuts at:      0     763    1279    2460 
   Size:        763     516    1181

  Fragments arranged by size: 

                1181     763     516
 
ApoI r'AATT_y

Cuts at:      0     275     712     779     925    1009    1375    1777    2104 
   Size:        275     437      67     146      84     366     402     327

Cuts at:   2104    2449    2460 
   Size:        345      11

  Fragments arranged by size: 

                 437     402     366     345     327     275     146      84
                  67      11
 
AseI AT'TA_AT

Cuts at:      0    1556    2460 
   Size:       1556     904
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1112    1145    1177    1210    2460 
   Size:       1112      33      32      33    1250

  Fragments arranged by size: 

                1250    1112      33      33      32
 
BamHI G'GATC_C

Cuts at:      0    1732    2091    2278    2305    2460 
   Size:       1732     359     187      27     155

  Fragments arranged by size: 

                1732     359     187     155      27
 
BanI G'GyrC_C

Cuts at:      0      67    2460 
   Size:         67    2393
 
BanII G_rGCy'C

Cuts at:      0    2185    2460 
   Size:       2185     275
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1451    2224    2460 
   Size:       1451     773     236

  Fragments arranged by size: 

                1451     773     236
 
BfrBI ATG'CAT

Cuts at:      0    1407    2460 
   Size:       1407    1053
 
Bme1580I G_kGCm'C

Cuts at:      0      70    1626    2460 
   Size:         70    1556     834

  Fragments arranged by size: 

                1556     834      70
 
BmtI G_CTAG'C

Cuts at:      0    1348    2460 
   Size:       1348    1112
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541    2276    2460 
   Size:        541    1735     184

  Fragments arranged by size: 

                1735     541     184
 
Bpu10I CC'TnA_GC

Cuts at:      0     244     764    2460 
   Size:        244     520    1696

  Fragments arranged by size: 

                1696     520     244
 
BsaAI yAC'GTr

Cuts at:      0      93    2460 
   Size:         93    2367
 
BsaBI GATnn'nnATC

Cuts at:      0    1274    2460 
   Size:       1274    1186
 
BsaHI Gr'CG_yC

Cuts at:      0     656    2460 
   Size:        656    1804
 
BsaWI w'CCGG_w

Cuts at:      0    1571    2460 
   Size:       1571     889
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1280    1310    2030    2060    2345    2375    2460 
   Size:       1280      30     720      30     285      30      85

  Fragments arranged by size: 

                1280     720     285      85      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     370    1304    2460 
   Size:        370     934    1156

  Fragments arranged by size: 

                1156     934     370
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550    2460 
   Size:        550    1910
 
BsiEI CG_ry'CG

Cuts at:      0      63    2460 
   Size:         63    2397
 
BsiHKAI G_wGCw'C

Cuts at:      0    1526    2460 
   Size:       1526     934
 
Bsp1286I G_dGCh'C

Cuts at:      0      70    1526    1626    2185    2460 
   Size:         70    1456     100     559     275

  Fragments arranged by size: 

                1456     559     275     100      70
 
BspEI T'CCGG_A

Cuts at:      0    1571    2460 
   Size:       1571     889
 
BsrBI CCG'CTC

Cuts at:      0      99    2460 
   Size:         99    2361
 
BsrFI r'CCGG_y

Cuts at:      0      47    2460 
   Size:         47    2413
 
BsrGI T'GTAC_A

Cuts at:      0      29    1860    2460 
   Size:         29    1831     600

  Fragments arranged by size: 

                1831     600      29
 
BssSI C'ACGA_G

Cuts at:      0    2359    2460 
   Size:       2359     101
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1404    1621    2460 
   Size:       1404     217     839

  Fragments arranged by size: 

                1404     839     217
 
BstBI TT'CG_AA

Cuts at:      0    1317    2460 
   Size:       1317    1143
 
BstYI r'GATC_y

Cuts at:      0     759    1275    1732    2091    2278    2287    2305    2460 
   Size:        759     516     457     359     187       9      18     155

  Fragments arranged by size: 

                 759     516     457     359     187     155      18       9
            
 
BtgI C'CryG_G

Cuts at:      0     317    1181    2460 
   Size:        317     864    1279

  Fragments arranged by size: 

                1279     864     317
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     362    2460 
   Size:        362    2098
 
BtsI GCAGTG_nn'

Cuts at:      0      58     176    1349    1415    2460 
   Size:         58     118    1173      66    1045

  Fragments arranged by size: 

                1173    1045     118      66      58
 
DraI TTT'AAA

Cuts at:      0     738    1004    1635    1872    2460 
   Size:        738     266     631     237     588

  Fragments arranged by size: 

                 738     631     588     266     237
 
EarI CTCTTCn'nnn_

Cuts at:      0     347     467    1962    1991    2460 
   Size:        347     120    1495      29     469

  Fragments arranged by size: 

                1495     469     347     120      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     239     371     541     659    2276    2460 
   Size:        124     115     132     170     118    1617     184

  Fragments arranged by size: 

                1617     184     170     132     124     118     115
 
EcoO109I rG'GnC_Cy

Cuts at:      0     309     333    2460 
   Size:        309      24    2127

  Fragments arranged by size: 

                2127     309      24
 
EcoRV GAT'ATC

Cuts at:      0    1566    1963    2460 
   Size:       1566     397     497

  Fragments arranged by size: 

                1566     497     397
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1123    2460 
   Size:       1123    1337
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     358     361     390     393    1138    1170    1181    1213 
   Size:        358       3      29       3     745      32      11      32

Cuts at:   1213    1280    1312    1786    1818    2460 
   Size:         67      32     474      32     642

  Fragments arranged by size: 

                 745     642     474     358      67      32      32      32
                  32      29      11       3       3
 
HincII GTy'rAC

Cuts at:      0     996    1329    1508    2460 
   Size:        996     333     179     952

  Fragments arranged by size: 

                 996     952     333     179
 
HindIII A'AGCT_T

Cuts at:      0    2194    2460 
   Size:       2194     266
 
HpaI GTT'AAC

Cuts at:      0     996    1508    2460 
   Size:        996     512     952

  Fragments arranged by size: 

                 996     952     512
 
MslI CAynn'nnrTG

Cuts at:      0     536     575    2460 
   Size:        536      39    1885

  Fragments arranged by size: 

                1885     536      39
 
NcoI C'CATG_G

Cuts at:      0    1181    2460 
   Size:       1181    1279
 
NdeI CA'TA_TG

Cuts at:      0    1259    2460 
   Size:       1259    1201
 
NheI G'CTAG_C

Cuts at:      0    1344    2460 
   Size:       1344    1116
 
NsiI A_TGCA'T

Cuts at:      0    1409    2460 
   Size:       1409    1051
 
NspI r_CATG'y

Cuts at:      0      79     114     855     909    1344    1411    1606    2460 
   Size:         79      35     741      54     435      67     195     854

  Fragments arranged by size: 

                 854     741     435     195      79      67      54      35
            
 
PciI A'CATG_T

Cuts at:      0     851    2460 
   Size:        851    1609
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1305    1485    2460 
   Size:       1305     180     975

  Fragments arranged by size: 

                1305     975     180
 
PfoI T'CCnGG_A

Cuts at:      0     518    2265    2301    2460 
   Size:        518    1747      36     159

  Fragments arranged by size: 

                1747     518     159      36
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     126     158    1568    1600    2460 
   Size:        126      32    1410      32     860

  Fragments arranged by size: 

                1410     860     126      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     309     333    2460 
   Size:        309      24    2127

  Fragments arranged by size: 

                2127     309      24
 
SfcI C'TryA_G

Cuts at:      0    1033    2045    2460 
   Size:       1033    1012     415

  Fragments arranged by size: 

                1033    1012     415
 
SphI G_CATG'C

Cuts at:      0    1606    2460 
   Size:       1606     854
 
SspI AAT'ATT

Cuts at:      0     555    2460 
   Size:        555    1905
 
StuI AGG'CCT

Cuts at:      0    2357    2460 
   Size:       2357     103
 
StyI C'CwwG_G

Cuts at:      0    1107    1181    2460 
   Size:       1107      74    1279

  Fragments arranged by size: 

                1279    1107      74
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      19     825    2460 
   Size:         19     806    1635

  Fragments arranged by size: 

                1635     806      19
 
TatI w'GTAC_w

Cuts at:      0      29    1860    2460 
   Size:         29    1831     600

  Fragments arranged by size: 

                1831     600      29
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      37      69    2345    2377    2460 
   Size:         37      32    2276      32      83

  Fragments arranged by size: 

                2276      83      37      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1068    2460 
   Size:       1068    1392
 
XmnI GAAnn'nnTTC

Cuts at:      0     746     926    2150    2460 
   Size:        746     180    1224     310

  Fragments arranged by size: 

                1224     746     310     180
 
ZraI GAC'GTC

Cuts at:      0     657    2460 
   Size:        657    1803
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AlfI    AlwNI     ApoI     AseI     BaeI
    BamHI     BanI    BanII     BbsI    BfrBI Bme1580I     BmtI     BpmI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     EarI
  Eco57MI EcoO109I    EcoRV     FalI    Hin4I   HincII  HindIII     HpaI
     MslI     NcoI     NdeI     NheI     NsiI     NspI     PciI    PflMI
     PfoI     PpiI    PpuMI     SfcI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
    BmgBI     BmrI     BplI    BpuEI     BsaI    BseYI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrDI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI    HaeII     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
   NgoMIV     NotI     NruI     PacI     PasI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SwaI     XbaI     XcmI     XhoI     XmaI
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