Restriction map for pF1KA1975
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AocI        ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        HgaI        HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII        1537  GACGTC               GACGT^C
AcyI         1537  GACGTC               G[GA]^CG[CT]C
Alw44I        112  GTGCAC               G^TGCAC
Aor51HI      1569  AGCGCT               AGC^GCT
ApaLI         112  GTGCAC               G^TGCAC
AvaI         1547  CCCGAG               C^[CT]CG[GA]G
AviII         571  TGCGCA               TGC^GCA
BamHI        1289  GGATCC               G^GATCC
BanI         1120  GGCACC               G^G[CT][GA]CC
BanII        1171  GAGCTC               G[GA]GC[CT]^C
BbsI          408  GAAGAC               GAAGAC(2/6)
BbvCI         802  GCTGAGG              CCTCAGC(-5/-2)
BcnI         1583  CCCGG                CC^[GC]GG
BglII          61  AGATCT               A^GATCT
BpuAI         408  GAAGAC               GAAGAC(2/6)
BsaHI        1537  GACGTC               G[GA]^CG[CT]C
BsmBI        1457  GAGACG               CGTCTC(1/5)
BsoBI        1547  CCCGAG               C^[CT]CG[GA]G
BstAPI       1359  GCACCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EarI         1273  GAAGAG               CTCTTC(1/4)
Eco47III     1569  AGCGCT               AGC^GCT
EcoNI         603  CCTATCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         646  GATATC               GAT^ATC
FspI          571  TGCGCA               TGC^GCA
HapII        1584  CCGG                 C^CGG
Hin1I        1537  GACGTC               G[GA]^CG[CT]C
HincII       1263  GTCAAC               GT[CT]^[GA]AC
HindII       1263  GTCAAC               GT[CT]^[GA]AC
HindIII       468  AAGCTT               A^AGCTT
HpaII        1584  CCGG                 C^CGG
Ksp632I      1273  GAAGAG               CTCTTC(1/4)
MslI         1608  CATCAACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1584  CCGG                 C^CGG
NciI         1583  CCCGG                CC^[GC]GG
PvuII         961  CAGCTG               CAG^CTG
SacI         1171  GAGCTC               GAGCT^C
SphI          377  GCATGC               GCATG^C
SspI          490  AATATT               AAT^ATT
StuI         1589  AGGCCT               AGG^CCT
TaqI          997  TCGA                 T^CGA
Tsp509I       640  AATT                 ^AATT
TthHB8I       997  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI         1527  GTAC                 GT^AC
             1626  GTAC                
BfaI          556  CTAG                 C^TAG
             1207  CTAG                
BglI          559  GCCACATCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1582  GCCCGGCAGGC         
BsaI         1039  GAGACC               GGTCTC(1/5)
             1441  GAGACC              
BsgI         1335  CTGCAC               GTGCAG(16/14)
             1464  CTGCAC              
BsrBI         348  GAGCGG               CCGCTC(-3/-3)
              880  GAGCGG              
BsrDI         596  GCAATG               GCAATG(2/0)
             1199  GCAATG              
BstXI         538  CCAGGAAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              608  CCAAGGACATGG        
DraI          147  TTTAAA               TTT^AAA
              859  TTTAAA              
DraII         197  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1385  AGGACCT             
DraIII        432  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1024  CACTGCGTG           
EcoO109I      197  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1385  AGGACCT             
HaeII        1470  GGCGCT               [GA]GCGC^[CT]
             1569  AGCGCT              
MaeI          556  CTAG                 C^TAG
             1207  CTAG                
MspA1I        961  CAGCTG               C[AC]G^C[GT]G
             1376  CCGCTG              
NcoI          257  CCATGG               C^CATGG
              983  CCATGG              
NdeI          628  CATATG               CA^TATG
              666  CATATG              
NspI          377  GCATGC               [GA]CATG^[CT]
             1007  ACATGC              
PpuMI         197  AGGACCT              [GA]G^G[AT]CC[CT]
             1385  AGGACCT             
RsaI         1527  GTAC                 GT^AC
             1626  GTAC                
TfiI         1047  GAATC                G^A[AT]TC
             1103  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AspHI       
AvaII       BalI        BbvI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PstI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
Tru9I       TseI        Tsp45I      TspRI       Van91I      
XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com