Restriction map for pF1KA1975
 (Linear) (Six-base) MAPSORT of: KIAA1975.seq  Check: 5077  from: 1  to: 1650



KIAA1975.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1370    1650 
   Size:       1370     280
 
AatII G_ACGT'C

Cuts at:      0    1541    1650 
   Size:       1541     109
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     657    1650 
   Size:        427     230     993

  Fragments arranged by size: 

                 993     427     230
 
AfeI AGC'GCT

Cuts at:      0    1571    1650 
   Size:       1571      79
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1630    1650 
   Size:       1630      20
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1274    1306    1650 
   Size:       1274      32     344

  Fragments arranged by size: 

                1274     344      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     918    1220    1362    1518    1650 
   Size:        918     302     142     156     132

  Fragments arranged by size: 

                 918     302     156     142     132
 
ApaLI G'TGCA_C

Cuts at:      0     112    1650 
   Size:        112    1538
 
AvaI C'yCGr_G

Cuts at:      0    1547    1650 
   Size:       1547     103
 
BamHI G'GATC_C

Cuts at:      0    1289    1650 
   Size:       1289     361
 
BanI G'GyrC_C

Cuts at:      0    1120    1650 
   Size:       1120     530
 
BanII G_rGCy'C

Cuts at:      0    1175    1650 
   Size:       1175     475
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     415    1650 
   Size:        415    1235
 
BbvCI CC'TCA_GC

Cuts at:      0     803    1650 
   Size:        803     847
 
BglI GCCn_nnn'nGGC

Cuts at:      0     565    1588    1650 
   Size:        565    1023      62

  Fragments arranged by size: 

                1023     565      62
 
BglII A'GATC_T

Cuts at:      0      61    1650 
   Size:         61    1589
 
Bme1580I G_kGCm'C

Cuts at:      0     116    1650 
   Size:        116    1534
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     674    1650 
   Size:        674     976
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     567     672    1168    1578    1650 
   Size:        343     224     105     496     410      72

  Fragments arranged by size: 

                 496     410     343     224     105      72
 
Bpu10I CC'TnA_GC

Cuts at:      0     471     803    1174    1650 
   Size:        471     332     371     476

  Fragments arranged by size: 

                 476     471     371     332
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1033    1435    1650 
   Size:       1033     402     215

  Fragments arranged by size: 

                1033     402     215
 
BsaHI Gr'CG_yC

Cuts at:      0    1538    1650 
   Size:       1538     112
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     145     175    1650 
   Size:        145      30    1475

  Fragments arranged by size: 

                1475     145      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     153     892    1444    1650 
   Size:        153     739     552     206

  Fragments arranged by size: 

                 739     552     206     153
 
BseYI C'CCAG_C

Cuts at:      0     181     654     959    1650 
   Size:        181     473     305     691

  Fragments arranged by size: 

                 691     473     305     181
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1320    1449    1650 
   Size:       1320     129     201

  Fragments arranged by size: 

                1320     201     129
 
BsiHKAI G_wGCw'C

Cuts at:      0     116     455    1175    1650 
   Size:        116     339     720     475

  Fragments arranged by size: 

                 720     475     339     116
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1451    1650 
   Size:       1451     199
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     455    1175    1650 
   Size:        116     339     720     475

  Fragments arranged by size: 

                 720     475     339     116
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     722    1370    1397    1650 
   Size:        722     648      27     253

  Fragments arranged by size: 

                 722     648     253      27
 
BsrBI CCG'CTC

Cuts at:      0     350     882    1650 
   Size:        350     532     768

  Fragments arranged by size: 

                 768     532     350
 
BsrDI GCAATG_nn'

Cuts at:      0     603    1206    1650 
   Size:        603     603     444

  Fragments arranged by size: 

                 603     603     444
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1365    1650 
   Size:       1365     285
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     545     615    1650 
   Size:        545      70    1035

  Fragments arranged by size: 

                1035     545      70
 
BstYI r'GATC_y

Cuts at:      0      61     914    1289    1650 
   Size:         61     853     375     361

  Fragments arranged by size: 

                 853     375     361      61
 
BtgI C'CryG_G

Cuts at:      0     257     983    1557    1650 
   Size:        257     726     574      93

  Fragments arranged by size: 

                 726     574     257      93
 
BtsI GCAGTG_nn'

Cuts at:      0     221     797    1023    1650 
   Size:        221     576     226     627

  Fragments arranged by size: 

                 627     576     226     221
 
DraI TTT'AAA

Cuts at:      0     149     861    1650 
   Size:        149     712     789

  Fragments arranged by size: 

                 789     712     149
 
DraIII CAC_nnn'GTG

Cuts at:      0     437    1029    1650 
   Size:        437     592     621

  Fragments arranged by size: 

                 621     592     437
 
EaeI y'GGCC_r

Cuts at:      0     547     843     920    1162    1650 
   Size:        547     296      77     242     488

  Fragments arranged by size: 

                 547     488     296     242      77
 
EarI CTCTTCn'nnn_

Cuts at:      0    1268    1650 
   Size:       1268     382
 
EcoICRI GAG'CTC

Cuts at:      0    1173    1650 
   Size:       1173     477
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     427     567     657     672    1168    1578    1650 
   Size:        343      84     140      90      15     496     410      72

  Fragments arranged by size: 

                 496     410     343     140      90      84      72      15
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     607    1650 
   Size:        607    1043
 
EcoO109I rG'GnC_Cy

Cuts at:      0     198    1386    1650 
   Size:        198    1188     264

  Fragments arranged by size: 

                1188     264     198
 
EcoRV GAT'ATC

Cuts at:      0     648    1650 
   Size:        648    1002
 
FspI TGC'GCA

Cuts at:      0     573    1650 
   Size:        573    1077
 
HaeII r_GCGC'y

Cuts at:      0    1474    1573    1650 
   Size:       1474      99      77

  Fragments arranged by size: 

                1474      99      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      18      50     775     807    1032    1064    1650 
   Size:         18      32     725      32     225      32     586

  Fragments arranged by size: 

                 725     586     225      32      32      32      18
 
HincII GTy'rAC

Cuts at:      0    1265    1650 
   Size:       1265     385
 
HindIII A'AGCT_T

Cuts at:      0     468    1650 
   Size:        468    1182
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     293    1650 
   Size:        293    1357
 
MscI TGG'CCA

Cuts at:      0     549     845     922    1164    1650 
   Size:        549     296      77     242     486

  Fragments arranged by size: 

                 549     486     296     242      77
 
MslI CAynn'nnrTG

Cuts at:      0    1612    1650 
   Size:       1612      38
 
MspA1I CmG'CkG

Cuts at:      0     963    1378    1650 
   Size:        963     415     272

  Fragments arranged by size: 

                 963     415     272
 
NcoI C'CATG_G

Cuts at:      0     257     983    1650 
   Size:        257     726     667

  Fragments arranged by size: 

                 726     667     257
 
NdeI CA'TA_TG

Cuts at:      0     629     667    1650 
   Size:        629      38     983

  Fragments arranged by size: 

                 983     629      38
 
NspI r_CATG'y

Cuts at:      0     381    1011    1650 
   Size:        381     630     639

  Fragments arranged by size: 

                 639     630     381
 
PasI CC'CwG_GG

Cuts at:      0    1350    1650 
   Size:       1350     300
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     263     918    1029    1650 
   Size:        263     655     111     621

  Fragments arranged by size: 

                 655     621     263     111
 
PpuMI rG'GwC_Cy

Cuts at:      0     198    1386    1650 
   Size:        198    1188     264

  Fragments arranged by size: 

                1188     264     198
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1080    1112    1650 
   Size:       1080      32     538

  Fragments arranged by size: 

                1080     538      32
 
PstI C_TGCA'G

Cuts at:      0     932     995    1625    1650 
   Size:        932      63     630      25

  Fragments arranged by size: 

                 932     630      63      25
 
PvuII CAG'CTG

Cuts at:      0     963    1650 
   Size:        963     687
 
SacI G_AGCT'C

Cuts at:      0    1175    1650 
   Size:       1175     475
 
SfcI C'TryA_G

Cuts at:      0     928     991    1621    1650 
   Size:        928      63     630      29

  Fragments arranged by size: 

                 928     630      63      29
 
SphI G_CATG'C

Cuts at:      0     381    1650 
   Size:        381    1269
 
SspI AAT'ATT

Cuts at:      0     492    1650 
   Size:        492    1158
 
StuI AGG'CCT

Cuts at:      0    1591    1650 
   Size:       1591      59
 
StyI C'CwwG_G

Cuts at:      0      20     257     608     983    1650 
   Size:         20     237     351     375     667

  Fragments arranged by size: 

                 667     375     351     237      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     249     591     646    1650 
   Size:        249     342      55    1004

  Fragments arranged by size: 

                1004     342     249      55
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1009    1068    1114    1407    1650 
   Size:       1009      59      46     293     243

  Fragments arranged by size: 

                1009     293     243      59      46
 
ZraI GAC'GTC

Cuts at:      0    1539    1650 
   Size:       1539     111
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI     AlfI     AloI    AlwNI    ApaLI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BglI    BglII
 Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI
   BstAPI    BstXI    BstYI     BtgI     BtsI     DraI   DraIII     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FspI    HaeII
    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NspI     PasI    PflMI    PpuMI     PsrI     PstI    PvuII
     SacI     SfcI     SphI     SspI     StuI     StyI    TaqII     XcmI
     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BspEI
    BspHI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DrdI     EagI     EciI    EcoRI
     FalI     FseI    FspAI     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com