Restriction map for
pF1KA1883
(Circular) (Six-base) MAPSORT of: pF1KA1883.seq Check: 8866 from: 1 to: 7493
pF1KA1883 7493 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 884 1299 1599 884
Size: 415 300 6778
Fragments arranged by size:
6778 415 300
AccI GT'mk_AC
Cuts at: 407 3521 4184 4531 407
Size: 3114 663 347 3369
Fragments arranged by size:
3369 3114 663 347
Acc65I G'GTAC_C
Cuts at: 4509 4509
Size: 7493
AclI AA'CG_TT
Cuts at: 7333 7333
Size: 7493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 781 1255 1289 5318 5750 6497 6765 353
Size: 428 474 34 4029 432 747 268 1081
Fragments arranged by size:
4029 1081 747 474 432 428 268 34
AfeI AGC'GCT
Cuts at: 3026 3026
Size: 7493
AflIII A'CryG_T
Cuts at: 4845 5949 6926 4845
Size: 1104 977 5412
Fragments arranged by size:
5412 1104 977
AgeI A'CCGG_T
Cuts at: 5881 5881
Size: 7493
AhdI GACnn_n'nnGTC
Cuts at: 3114 3114
Size: 7493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 940 1939 940
Size: 999 6494
AlwNI CAG_nnn'CTG
Cuts at: 396 504 696 1312 2694 3169 3322 3418 4798
Size: 108 192 616 1382 475 153 96 1380
Cuts at: 4798 6365 396
Size: 1567 1524
Fragments arranged by size:
1567 1524 1382 1380 616 475 192 153
108 96
ApaI G_GGCC'C
Cuts at: 623 1152 2394 2470 2648 2952 3194 3648 3721
Size: 529 1242 76 178 304 242 454 73
Cuts at: 3721 3850 4015 4455 623
Size: 129 165 440 3661
Fragments arranged by size:
3661 1242 529 454 440 304 242 178
165 129 76 73
ApaLI G'TGCA_C
Cuts at: 895 6263 895
Size: 5368 2125
ApoI r'AATT_y
Cuts at: 319 4497 6846 7102 319
Size: 4178 2349 256 710
Fragments arranged by size:
4178 2349 710 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7493
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7493
AvaI C'yCGr_G
Cuts at: 790 818 1042 1051 1385 1437 1537 1942 2218
Size: 28 224 9 334 52 100 405 276
Cuts at: 2218 2296 2341 2387 2581 2622 2640 2648 2753
Size: 78 45 46 194 41 18 8 105
Cuts at: 2753 2788 2808 2962 3208 3364 3385 3433 3603
Size: 35 20 154 246 156 21 48 170
Cuts at: 3603 3628 3640 3706 3721 3837 4015 4237 4434
Size: 25 12 66 15 116 178 222 197
Cuts at: 4434 4448 4513 790
Size: 14 65 3770
Fragments arranged by size:
3770 405 334 276 246 224 222 197
194 178 170 156 154 116 105 100
78 66 65 52 48 46 45 41
35 28 25 21 20 18 15 14
12 9 8
BamHI G'GATC_C
Cuts at: 1019 4518 1019
Size: 3499 3994
BanI G'GyrC_C
Cuts at: 121 1038 1587 1954 2328 3399 3984 4038 4509
Size: 917 549 367 374 1071 585 54 471
Cuts at: 4509 4923 5171 5206 121
Size: 414 248 35 2408
Fragments arranged by size:
2408 1071 917 585 549 471 414 374
367 248 54 35
BanII G_rGCy'C
Cuts at: 207 605 623 780 1049 1061 1152 1760 2254
Size: 398 18 157 269 12 91 608 494
Cuts at: 2254 2394 2470 2577 2648 2916 2952 3186 3194
Size: 140 76 107 71 268 36 234 8
Cuts at: 3194 3273 3384 3411 3453 3641 3648 3721 3850
Size: 79 111 27 42 188 7 73 129
Cuts at: 3850 4015 4455 4507 5537 207
Size: 165 440 52 1030 2163
Fragments arranged by size:
2163 1030 608 494 440 398 269 268
234 188 165 157 140 129 111 107
91 79 76 73 71 52 42 36
27 18 12 8 7
BbeI G_GCGC'C
Cuts at: 125 1042 1958 2332 3403 3988 4927 5175 125
Size: 917 916 374 1071 585 939 248 2443
Fragments arranged by size:
2443 1071 939 917 916 585 374 248
BbsI GAAGACnn'nnnn_
Cuts at: 2069 3208 2069
Size: 1139 6354
BbvCI CC'TCA_GC
Cuts at: 425 1905 3043 425
Size: 1480 1138 4875
Fragments arranged by size:
4875 1480 1138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 7459
BciVI GTATCCnnnnn_n'
Cuts at: 5391 6152 7493 5391
Size: 761 1341 5391
Fragments arranged by size:
5391 1341 761
BclI T'GATC_A
Cuts at: 5013 5013
Size: 7493
BglI GCCn_nnn'nGGC
Cuts at: 1359 1618 1810 1819 3332 3415 3585 3886 3907
Size: 259 192 9 1513 83 170 301 21
Cuts at: 3907 1359
Size: 4945
Fragments arranged by size:
4945 1513 301 259 192 170 83 21
9
BglII A'GATC_T
Cuts at: 5008 7045 5008
Size: 2037 5456
BlpI GC'TnA_GC
Cuts at: 1927 4219 4605 1927
Size: 2292 386 4815
Fragments arranged by size:
4815 2292 386
Bme1580I G_kGCm'C
Cuts at: 623 899 1152 1590 1802 2394 2470 2648 2952
Size: 276 253 438 212 592 76 178 304
Cuts at: 2952 3194 3463 3648 3721 3850 4015 4043 4455
Size: 242 269 185 73 129 165 28 412
Cuts at: 4455 5118 5211 6267 623
Size: 663 93 1056 1849
Fragments arranged by size:
1849 1056 663 592 438 412 304 276
269 253 242 212 185 178 165 129
93 76 73 28
BmgBI CAC'GTC
Cuts at: 4176 4176
Size: 7493
BmrI ACTGGGnnnn_n'
Cuts at: 760 1785 1893 2328 2866 3179 3401 3448 4705
Size: 1025 108 435 538 313 222 47 1257
Cuts at: 4705 4786 5121 7265 760
Size: 81 335 2144 988
Fragments arranged by size:
2144 1257 1025 988 538 435 335 313
222 108 81 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 363 396 399 582 1761 2067 2244 2268 5860
Size: 33 3 183 1179 306 177 24 3592
Cuts at: 5860 363
Size: 1996
Fragments arranged by size:
3592 1996 1179 306 183 177 33 24
3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 673 871 1502 2176 2600 2914 3004 3049
Size: 27 198 631 674 424 314 90 45
Cuts at: 3049 3283 3610 4159 4250 4364 4447 646
Size: 234 327 549 91 114 83 3692
Fragments arranged by size:
3692 674 631 549 424 327 314 234
198 114 91 90 83 45 27
Bpu10I CC'TnA_GC
Cuts at: 425 490 505 806 1754 1905 2863 3043 425
Size: 65 15 301 948 151 958 180 4875
Fragments arranged by size:
4875 958 948 301 180 151 65 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 4081 4669 5548 6040 6338 1219
Size: 2862 588 879 492 298 2374
Fragments arranged by size:
2862 2374 879 588 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 370 435 815 1846 1957 1970 2488 2494 3247
Size: 65 380 1031 111 13 518 6 753
Cuts at: 3247 3297 4164 4423 7197 370
Size: 50 867 259 2774 666
Fragments arranged by size:
2774 1031 867 753 666 518 380 259
111 65 50 13 6
BsaAI yAC'GTr
Cuts at: 894 4846 894
Size: 3952 3541
BsaBI GATnn'nnATC
Cuts at: 6 5007 7044 6
Size: 5001 2037 455
Fragments arranged by size:
5001 2037 455
BsaHI Gr'CG_yC
Cuts at: 122 166 1039 1606 1955 2168 2329 3205 3400
Size: 44 873 567 349 213 161 876 195
Cuts at: 3400 3878 3985 4204 4460 4924 5172 5874 7370
Size: 478 107 219 256 464 248 702 1496
Cuts at: 7370 122
Size: 245
Fragments arranged by size:
1496 876 873 702 567 478 464 349
256 248 245 219 213 195 161 107
44
BsaWI w'CCGG_w
Cuts at: 3567 4682 5203 5881 6155 6302 6713 3567
Size: 1115 521 678 274 147 411 4347
Fragments arranged by size:
4347 1115 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1327 1357 3031 3061 1327
Size: 30 1674 30 5759
Fragments arranged by size:
5759 1674 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 197 206 262 265 372 375 388 1039 1192
Size: 9 56 3 107 3 13 651 153
Cuts at: 1192 1440 1750 2047 2050 2053 2056 2220 2375
Size: 248 310 297 3 3 3 164 155
Cuts at: 2375 2563 2941 3031 3285 3390 3597 3946 3976
Size: 188 378 90 254 105 207 349 30
Cuts at: 3976 3979 3988 4360 197
Size: 3 9 372 3330
Fragments arranged by size:
3330 651 378 372 349 310 297 254
248 207 188 164 155 153 107 105
90 56 30 13 9 9 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 779 1031 1151 1295 1669 1838 1893 1934 2180
Size: 252 120 144 374 169 55 41 246
Cuts at: 2180 3280 3336 3418 4315 4918 5676 6253 6749
Size: 1100 56 82 897 603 758 577 496
Cuts at: 6749 779
Size: 1523
Fragments arranged by size:
1523 1100 897 758 603 577 496 374
252 246 169 144 120 82 56 55
41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1403 2142 3260 1403
Size: 739 1118 5636
Fragments arranged by size:
5636 1118 739
BsiEI CG_ry'CG
Cuts at: 104 143 1228 1575 2408 2432 2546 2569 3236
Size: 39 1085 347 833 24 114 23 667
Cuts at: 3236 5035 5081 6289 104
Size: 1799 46 1208 1308
Fragments arranged by size:
1799 1308 1208 1085 833 667 347 114
46 39 24 23
BsiHKAI G_wGCw'C
Cuts at: 605 899 1049 1061 1505 1742 2683 4507 5285
Size: 294 150 12 444 237 941 1824 778
Cuts at: 5285 5475 6267 605
Size: 190 792 1831
Fragments arranged by size:
1831 1824 941 792 778 444 294 237
190 150 12
BsmI GAATG_Cn'
Cuts at: 879 879
Size: 7493
BsmBI CGTCTCn'nnnn_
Cuts at: 150 706 1382 3680 4195 4419 150
Size: 556 676 2298 515 224 3224
Fragments arranged by size:
3224 2298 676 556 515 224
Bsp1286I G_dGCh'C
Cuts at: 207 605 623 780 899 1049 1061 1152 1505
Size: 398 18 157 119 150 12 91 353
Cuts at: 1505 1590 1742 1760 1802 2254 2394 2470 2577
Size: 85 152 18 42 452 140 76 107
Cuts at: 2577 2648 2683 2916 2952 3186 3194 3273 3384
Size: 71 35 233 36 234 8 79 111
Cuts at: 3384 3411 3453 3463 3641 3648 3721 3850 4015
Size: 27 42 10 178 7 73 129 165
Cuts at: 4015 4043 4455 4507 5118 5211 5285 5475 5537
Size: 28 412 52 611 93 74 190 62
Cuts at: 5537 6267 207
Size: 730 1433
Fragments arranged by size:
1433 730 611 452 412 398 353 234
233 190 178 165 157 152 150 140
129 119 111 107 93 91 85 79
76 74 73 71 62 52 42 42
36 35 28 27 18 18 12 10
8 7
BspEI T'CCGG_A
Cuts at: 6713 6713
Size: 7493
BspHI T'CATG_A
Cuts at: 6669 6669
Size: 7493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 521 833 884 1299 1599 4543 4757 5059 5440
Size: 312 51 415 300 2944 214 302 381
Cuts at: 5440 521
Size: 2574
Fragments arranged by size:
2944 2574 415 381 312 302 300 214
51
BsrBI CCG'CTC
Cuts at: 50 1350 1931 2001 2160 2247 2362 2631 3819
Size: 1300 581 70 159 87 115 269 1188
Cuts at: 3819 5785 5839 7323 7488 50
Size: 1966 54 1484 165 55
Fragments arranged by size:
1966 1484 1300 1188 581 269 165 159
115 87 70 55 54
BsrDI GCAATG_nn'
Cuts at: 5405 5405
Size: 7493
BsrFI r'CCGG_y
Cuts at: 494 1570 1602 3661 3901 3924 5491 5672 5881
Size: 1076 32 2059 240 23 1567 181 209
Cuts at: 5881 494
Size: 2106
Fragments arranged by size:
2106 2059 1567 1076 240 209 181 32
23
BssSI C'ACGA_G
Cuts at: 3528 5764 6122 3528
Size: 2236 358 4899
Fragments arranged by size:
4899 2236 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 4870 1426
Size: 3444 4049
BstBI TT'CG_AA
Cuts at: 5854 5854
Size: 7493
BstEII G'GTnAC_C
Cuts at: 1119 1509 1119
Size: 390 7103
BstXI CCAn_nnnn'nTGG
Cuts at: 184 483 1031 3194 3288 184
Size: 299 548 2163 94 4389
Fragments arranged by size:
4389 2163 548 299 94
BstYI r'GATC_y
Cuts at: 1 1019 4518 4985 5008 5343 5589 6601 6687
Size: 1018 3499 467 23 335 246 1012 86
Cuts at: 6687 6699 7045 1
Size: 12 346 449
Fragments arranged by size:
3499 1018 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1808 3758 1808
Size: 1950 5543
BtgI C'CryG_G
Cuts at: 861 1062 1524 2014 2270 2396 2432 2569 3850
Size: 201 462 490 256 126 36 137 1281
Cuts at: 3850 3862 4006 4167 4478 861
Size: 12 144 161 311 3876
Fragments arranged by size:
3876 1281 490 462 311 256 201 161
144 137 126 36 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 317 5443 5624 5787 317
Size: 5126 181 163 2023
Fragments arranged by size:
5126 2023 181 163
BtsI GCAGTG_nn'
Cuts at: 1024 3293 4736 1024
Size: 2269 1443 3781
Fragments arranged by size:
3781 2269 1443
DraI TTT'AAA
Cuts at: 4492 4492
Size: 7493
DraIII CAC_nnn'GTG
Cuts at: 1426 1426
Size: 7493
DrdI GACnn_nn'nnGTC
Cuts at: 1180 2830 4180 5199 6057 1180
Size: 1650 1350 1019 858 2616
Fragments arranged by size:
2616 1650 1350 1019 858
EaeI y'GGCC_r
Cuts at: 140 1225 1285 1572 2258 2405 2429 2435 2543
Size: 1085 60 287 686 147 24 6 108
Cuts at: 2543 2566 2608 5078 5252 5643 5670 7015 140
Size: 23 42 2470 174 391 27 1345 618
Fragments arranged by size:
2470 1345 1085 686 618 391 287 174
147 108 60 42 27 24 23 6
EagI C'GGCC_G
Cuts at: 140 1225 1572 2405 2429 2543 2566 5078 140
Size: 1085 347 833 24 114 23 2512 2555
Fragments arranged by size:
2555 2512 1085 833 347 114 24 23
EarI CTCTTCn'nnn_
Cuts at: 1768 1876 2024 2966 4195 5516 5726 1768
Size: 108 148 942 1229 1321 210 3535
Fragments arranged by size:
3535 1321 1229 942 210 148 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 138 1189 2953 3680 3943 4369 5701 6011 6157
Size: 1051 1764 727 263 426 1332 310 146
Cuts at: 6157 7303 138
Size: 1146 328
Fragments arranged by size:
1764 1332 1146 1051 727 426 328 310
263 146
EcoICRI GAG'CTC
Cuts at: 603 1047 1059 4505 603
Size: 444 12 3446 3591
Fragments arranged by size:
3591 3446 444 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 646 673 781 871 1255 1289 1502 2176
Size: 293 27 108 90 384 34 213 674
Cuts at: 2176 2600 2914 3004 3049 3283 3610 4159 4250
Size: 424 314 90 45 234 327 549 91
Cuts at: 4250 4364 4447 5318 5750 6497 6765 353
Size: 114 83 871 432 747 268 1081
Fragments arranged by size:
1081 871 747 674 549 432 424 384
327 314 293 268 234 213 114 108
91 90 90 83 45 34 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2707 2707
Size: 7493
EcoO109I rG'GnC_Cy
Cuts at: 619 620 756 795 1067 1148 1182 1442 1779
Size: 1 136 39 272 81 34 260 337
Cuts at: 1779 1812 1821 1833 1938 2101 2135 2391 2467
Size: 33 9 12 105 163 34 256 76
Cuts at: 2467 2559 2644 2645 2764 2785 2823 2948 2949
Size: 92 85 1 119 21 38 125 1
Cuts at: 2949 2957 3047 3084 3129 3190 3191 3644 3645
Size: 8 90 37 45 61 1 453 1
Cuts at: 3645 3693 3717 3718 3751 4011 4012 4230 4384
Size: 48 24 1 33 260 1 218 154
Cuts at: 4384 4452 4474 4632 619
Size: 68 22 158 3480
Fragments arranged by size:
3480 453 337 272 260 260 256 218
163 158 154 136 125 119 105 92
90 85 81 76 68 61 48 45
39 38 37 34 34 33 33 24
22 21 12 9 8 1 1 1
1 1 1 1
EcoRI G'AATT_C
Cuts at: 4497 4497
Size: 7493
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2679 5512 2679
Size: 2833 4660
FseI GG_CCGG'CC
Cuts at: 1574 1574
Size: 7493
FspI TGC'GCA
Cuts at: 916 949 5274 916
Size: 33 4325 3135
Fragments arranged by size:
4325 3135 33
FspAI rTGC'GCAy
Cuts at: 949 949
Size: 7493
HaeII r_GCGC'y
Cuts at: 125 1014 1042 1302 1660 1694 1958 2332 2605
Size: 889 28 260 358 34 264 374 273
Cuts at: 2605 3028 3403 3601 3811 3919 3988 4061 4273
Size: 423 375 198 210 108 69 73 212
Cuts at: 4273 4927 5175 6197 7176 125
Size: 654 248 1022 979 442
Fragments arranged by size:
1022 979 889 654 442 423 375 374
358 273 264 260 248 212 210 198
108 73 69 34 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 784 816 1645 1677 2215 2247 2789 2821 3031
Size: 32 829 32 538 32 542 32 210
Cuts at: 3031 3063 3100 3132 3204 3236 5335 5365 5367
Size: 32 37 32 72 32 2099 30 2
Cuts at: 5367 5397 784
Size: 30 2880
Fragments arranged by size:
2880 2099 829 542 538 210 72 37
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 748 4259 4532 748
Size: 3511 273 3709
Fragments arranged by size:
3709 3511 273
KasI G'GCGC_C
Cuts at: 121 1038 1954 2328 3399 3984 4923 5171 121
Size: 917 916 374 1071 585 939 248 2443
Fragments arranged by size:
2443 1071 939 917 916 585 374 248
KpnI G_GTAC'C
Cuts at: 4513 4513
Size: 7493
MfeI C'AATT_G
Cuts at: 1321 1321
Size: 7493
MluI A'CGCG_T
Cuts at: 6926 6926
Size: 7493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 593 1664 6164 6348 593
Size: 1071 4500 184 1738
Fragments arranged by size:
4500 1738 1071 184
MscI TGG'CCA
Cuts at: 2437 5254 2437
Size: 2817 4676
MslI CAynn'nnrTG
Cuts at: 182 680 1078 5482 5609 182
Size: 498 398 4404 127 2066
Fragments arranged by size:
4404 2066 498 398 127
MspA1I CmG'CkG
Cuts at: 420 783 863 1526 1842 2398 2486 2571 2691
Size: 363 80 663 316 556 88 85 120
Cuts at: 2691 3555 3683 3852 4008 4083 4328 4392 4604
Size: 864 128 169 156 75 245 64 212
Cuts at: 4604 4918 5278 6291 6536 420
Size: 314 360 1013 245 1377
Fragments arranged by size:
1377 1013 864 663 556 363 360 316
314 245 245 212 169 156 128 120
88 85 80 75 64
NaeI GCC'GGC
Cuts at: 496 1572 1604 3663 3903 3926 5674 496
Size: 1076 32 2059 240 23 1748 2315
Fragments arranged by size:
2315 2059 1748 1076 240 32 23
NarI GG'CG_CC
Cuts at: 122 1039 1955 2329 3400 3985 4924 5172 122
Size: 917 916 374 1071 585 939 248 2443
Fragments arranged by size:
2443 1071 939 917 916 585 374 248
NcoI C'CATG_G
Cuts at: 2270 2270
Size: 7493
NgoMIV G'CCGG_C
Cuts at: 494 1570 1602 3661 3901 3924 5672 494
Size: 1076 32 2059 240 23 1748 2315
Fragments arranged by size:
2315 2059 1748 1076 240 32 23
NspI r_CATG'y
Cuts at: 1213 4546 5046 5953 1213
Size: 3333 500 907 2753
Fragments arranged by size:
3333 2753 907 500
PasI CC'CwG_GG
Cuts at: 195 483 1113 2056 2952 3187 3194 3412 195
Size: 288 630 943 896 235 7 218 4276
Fragments arranged by size:
4276 943 896 630 288 235 218 7
PciI A'CATG_T
Cuts at: 5949 5949
Size: 7493
PflMI CCAn_nnn'nTGG
Cuts at: 591 696 1775 3169 3418 591
Size: 105 1079 1394 249 4666
Fragments arranged by size:
4666 1394 1079 249 105
PfoI T'CCnGG_A
Cuts at: 3298 3298
Size: 7493
PmeI GTTT'AAAC
Cuts at: 4492 4492
Size: 7493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6632 6664 6632
Size: 32 7461
PpuMI rG'GwC_Cy
Cuts at: 756 1067 1182 1442 1779 1833 1938 2101 2559
Size: 311 115 260 337 54 105 163 458
Cuts at: 2559 2764 2823 2957 3047 3693 3751 756
Size: 205 59 134 90 646 58 4498
Fragments arranged by size:
4498 646 458 337 311 260 205 163
134 115 105 90 59 58 54
PshAI GACnn'nnGTC
Cuts at: 2624 2624
Size: 7493
PspOMI G'GGCC_C
Cuts at: 619 1148 2390 2466 2644 2948 3190 3644 3717
Size: 529 1242 76 178 304 242 454 73
Cuts at: 3717 3846 4011 4451 619
Size: 129 165 440 3661
Fragments arranged by size:
3661 1242 529 454 440 304 242 178
165 129 76 73
PspXI vC'TCGA_Gb
Cuts at: 1537 1537
Size: 7493
PstI C_TGCA'G
Cuts at: 518 674 839 4540 5225 518
Size: 156 165 3701 685 2786
Fragments arranged by size:
3701 2786 685 165 156
PvuI CG_AT'CG
Cuts at: 104 3236 104
Size: 3132 4361
PvuII CAG'CTG
Cuts at: 1842 2691 4918 5278 1842
Size: 849 2227 360 4057
Fragments arranged by size:
4057 2227 849 360
RsrII CG'GwC_CG
Cuts at: 5688 5688
Size: 7493
SacI G_AGCT'C
Cuts at: 605 1049 1061 4507 605
Size: 444 12 3446 3591
Fragments arranged by size:
3591 3446 444 12
SacII CC_GC'GG
Cuts at: 864 1527 2399 2572 3853 4009 864
Size: 663 872 173 1281 156 4348
Fragments arranged by size:
4348 1281 872 663 173 156
SalI G'TCGA_C
Cuts at: 4530 4530
Size: 7493
SanDI GG'GwC_CC
Cuts at: 1442 1779 1938 2101 2559 2823 2957 3693 3751
Size: 337 159 163 458 264 134 736 58
Cuts at: 3751 1442
Size: 5184
Fragments arranged by size:
5184 736 458 337 264 163 159 134
58
SapI GCTCTTCn'nnn_
Cuts at: 5516 5726 5516
Size: 210 7283
SbfI CC_TGCA'GG
Cuts at: 518 4540 518
Size: 4022 3471
ScaI AGT'ACT
Cuts at: 1720 7010 1720
Size: 5290 2203
SfcI C'TryA_G
Cuts at: 33 514 670 835 4536 5221 6214 6405 33
Size: 481 156 165 3701 685 993 191 1121
Fragments arranged by size:
3701 1121 993 685 481 191 165 156
SfiI GGCCn_nnn'nGGCC
Cuts at: 1359 1618 1819 3332 1359
Size: 259 201 1513 5520
Fragments arranged by size:
5520 1513 259 201
SfoI GGC'GCC
Cuts at: 123 1040 1956 2330 3401 3986 4925 5173 123
Size: 917 916 374 1071 585 939 248 2443
Fragments arranged by size:
2443 1071 939 917 916 585 374 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7493
SmaI CCC'GGG
Cuts at: 1439 2343 2624 2642 2650 2755 2790 2810 3366
Size: 904 281 18 8 105 35 20 556
Cuts at: 3366 3642 3839 4017 4436 4450 4515 1439
Size: 276 197 178 419 14 65 4417
Fragments arranged by size:
4417 904 556 419 281 276 197 178
105 65 35 20 18 14 8
SmlI C'TyrA_G
Cuts at: 818 1234 1537 4096 4648 5563 6055 6317 818
Size: 416 303 2559 552 915 492 262 1994
Fragments arranged by size:
2559 1994 915 552 492 416 303 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7493
SphI G_CATG'C
Cuts at: 4546 5046 4546
Size: 500 6993
SrfI GCCC'GGGC
Cuts at: 4450 4450
Size: 7493
StuI AGG'CCT
Cuts at: 2453 2453
Size: 7493
StyI C'CwwG_G
Cuts at: 2076 2270 3007 3175 3330 4627 4981 2076
Size: 194 737 168 155 1297 354 4588
Fragments arranged by size:
4588 1297 737 354 194 168 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2007 2512 3294 3385 5076 5209 5668 5877 2007
Size: 505 782 91 1691 133 459 209 3623
Fragments arranged by size:
3623 1691 782 505 459 209 133 91
TatI w'GTAC_w
Cuts at: 356 1718 7008 356
Size: 1362 5290 841
Fragments arranged by size:
5290 1362 841
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 865 897 3482 3514 7010 7042 865
Size: 32 2585 32 3496 32 1316
Fragments arranged by size:
3496 2585 1316 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5290 5290
Size: 7493
XbaI T'CTAG_A
Cuts at: 4524 4524
Size: 7493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1013 2197 3618 4988 1013
Size: 1184 1421 1370 3518
Fragments arranged by size:
3518 1421 1370 1184
XhoI C'TCGA_G
Cuts at: 818 1537 818
Size: 719 6774
XmaI C'CCGG_G
Cuts at: 1437 2341 2622 2640 2648 2753 2788 2808 3364
Size: 904 281 18 8 105 35 20 556
Cuts at: 3364 3640 3837 4015 4434 4448 4513 1437
Size: 276 197 178 419 14 65 4417
Fragments arranged by size:
4417 904 556 419 281 276 197 178
105 65 35 20 18 14 8
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AflII AleI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsrGI BssHII BstZ17I ClaI CspCI EcoRV
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PmlI PsiI PsrI SexAI SgrAI SnaBI SspI SwaI
XmnI ZraI