Restriction map for pF1KA1692
 (Linear) (Six-base) MAPSORT of: KIAA1692.seq  Check: 7190  from: 1  to: 2499



KIAA1692.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1780    2499 
   Size:       1780     719
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119    1502    1801    2052    2499 
   Size:        119    1383     299     251     447

  Fragments arranged by size: 

                1383     447     299     251     119
 
AflIII A'CryG_T

Cuts at:      0    2057    2499 
   Size:       2057     442
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2109    2499 
   Size:       2109     390
 
AlwNI CAG_nnn'CTG

Cuts at:      0     920    1492    1570    1714    1878    2499 
   Size:        920     572      78     144     164     621

  Fragments arranged by size: 

                 920     621     572     164     144      78
 
ApaI G_GGCC'C

Cuts at:      0    1030    2499 
   Size:       1030    1469
 
ApoI r'AATT_y

Cuts at:      0     403    1150    1557    2499 
   Size:        403     747     407     942

  Fragments arranged by size: 

                 942     747     407     403
 
AseI AT'TA_AT

Cuts at:      0     587    2499 
   Size:        587    1912
 
AvaI C'yCGr_G

Cuts at:      0     691    2354    2499 
   Size:        691    1663     145

  Fragments arranged by size: 

                1663     691     145
 
BamHI G'GATC_C

Cuts at:      0    1847    2499 
   Size:       1847     652
 
BanII G_rGCy'C

Cuts at:      0     254    1030    1932    2499 
   Size:        254     776     902     567

  Fragments arranged by size: 

                 902     776     567     254
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      92     308    1205    1244    1508    1739    1948    2499 
   Size:         92     216     897      39     264     231     209     551

  Fragments arranged by size: 

                 897     551     264     231     216     209      92      39
            
 
BbvCI CC'TCA_GC

Cuts at:      0     578    1663    1838    2499 
   Size:        578    1085     175     661

  Fragments arranged by size: 

                1085     661     578     175
 
BclI T'GATC_A

Cuts at:      0     492    1716    2499 
   Size:        492    1224     783

  Fragments arranged by size: 

                1224     783     492
 
BfrBI ATG'CAT

Cuts at:      0       3    2499 
   Size:          3    2496
 
BglII A'GATC_T

Cuts at:      0      36     480    2499 
   Size:         36     444    2019

  Fragments arranged by size: 

                2019     444      36
 
Bme1580I G_kGCm'C

Cuts at:      0    1030    2499 
   Size:       1030    1469
 
BmgBI CAC'GTC

Cuts at:      0    2058    2499 
   Size:       2058     441
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     364    2499 
   Size:        364    2135
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1006    1583    2416    2499 
   Size:       1006     577     833      83

  Fragments arranged by size: 

                1006     833     577      83
 
Bpu10I CC'TnA_GC

Cuts at:      0     578    1663    1838    2499 
   Size:        578    1085     175     661

  Fragments arranged by size: 

                1085     661     578     175
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     205     276    2499 
   Size:        205      71    2223

  Fragments arranged by size: 

                2223     205      71
 
BsaBI GATnn'nnATC

Cuts at:      0    1852    2499 
   Size:       1852     647
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      46      76     949     979     997    1027    1978    2008 
   Size:         46      30     873      30      18      30     951      30

Cuts at:   2008    2499 
   Size:        491

  Fragments arranged by size: 

                 951     873     491      46      30      30      30      30
                  18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     299    1005    2499 
   Size:        299     706    1494

  Fragments arranged by size: 

                1494     706     299
 
BseYI C'CCAG_C

Cuts at:      0      49    1506    2499 
   Size:         49    1457     993

  Fragments arranged by size: 

                1457     993      49
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     453    2499 
   Size:        453    2046
 
BsiHKAI G_wGCw'C

Cuts at:      0    1932    1962    2499 
   Size:       1932      30     537

  Fragments arranged by size: 

                1932     537      30
 
BsmI GAATG_Cn'

Cuts at:      0     960    2421    2499 
   Size:        960    1461      78

  Fragments arranged by size: 

                1461     960      78
 
Bsp1286I G_dGCh'C

Cuts at:      0     254    1030    1932    1962    2499 
   Size:        254     776     902      30     537

  Fragments arranged by size: 

                 902     776     537     254      30
 
BspHI T'CATG_A

Cuts at:      0     335    2499 
   Size:        335    2164
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     564    1780    2499 
   Size:        564    1216     719

  Fragments arranged by size: 

                1216     719     564
 
BsrBI CCG'CTC

Cuts at:      0    2345    2499 
   Size:       2345     154
 
BsrDI GCAATG_nn'

Cuts at:      0     392    1073    2098    2499 
   Size:        392     681    1025     401

  Fragments arranged by size: 

                1025     681     401     392
 
BssSI C'ACGA_G

Cuts at:      0      44    2499 
   Size:         44    2455
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1957    2499 
   Size:       1957     542
 
BstBI TT'CG_AA

Cuts at:      0    2363    2499 
   Size:       2363     136
 
BstYI r'GATC_y

Cuts at:      0      36     480    1847    2010    2499 
   Size:         36     444    1367     163     489

  Fragments arranged by size: 

                1367     489     444     163      36
 
BtgI C'CryG_G

Cuts at:      0    1685    1843    2499 
   Size:       1685     158     656

  Fragments arranged by size: 

                1685     656     158
 
BtsI GCAGTG_nn'

Cuts at:      0     404    2499 
   Size:        404    2095
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1358    1393    2499 
   Size:       1358      35    1106

  Fragments arranged by size: 

                1358    1106      35
 
EaeI y'GGCC_r

Cuts at:      0     947    1455    2142    2324    2499 
   Size:        947     508     687     182     175

  Fragments arranged by size: 

                 947     687     508     182     175
 
EarI CTCTTCn'nnn_

Cuts at:      0      64     899    2499 
   Size:         64     835    1600

  Fragments arranged by size: 

                1600     835      64
 
EcoICRI GAG'CTC

Cuts at:      0    1930    2499 
   Size:       1930     569
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119    1006    1502    1583    1801    2052    2416    2499 
   Size:        119     887     496      81     218     251     364      83

  Fragments arranged by size: 

                 887     496     364     251     218     119      83      81
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     240    1027    2499 
   Size:         54     186     787    1472

  Fragments arranged by size: 

                1472     787     186      54
 
EcoRI G'AATT_C

Cuts at:      0    1150    2499 
   Size:       1150    1349
 
EcoRV GAT'ATC

Cuts at:      0     333    2499 
   Size:        333    2166
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     109    1367    1659    1782    2182    2499 
   Size:        109    1258     292     123     400     317

  Fragments arranged by size: 

                1258     400     317     292     123     109
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78     202     234     997    1029    1546    1578 
   Size:         46      32     124      32     763      32     517      32

Cuts at:   1578    2499 
   Size:        921

  Fragments arranged by size: 

                 921     763     517     124      46      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     101    2499 
   Size:        101    2398
 
MscI TGG'CCA

Cuts at:      0     949    1457    2144    2326    2499 
   Size:        949     508     687     182     173

  Fragments arranged by size: 

                 949     687     508     182     173
 
MslI CAynn'nnrTG

Cuts at:      0     397     899    2091    2443    2499 
   Size:        397     502    1192     352      56

  Fragments arranged by size: 

                1192     502     397     352      56
 
MspA1I CmG'CkG

Cuts at:      0     627    1506    2499 
   Size:        627     879     993

  Fragments arranged by size: 

                 993     879     627
 
NcoI C'CATG_G

Cuts at:      0    1685    1843    2499 
   Size:       1685     158     656

  Fragments arranged by size: 

                1685     656     158
 
NdeI CA'TA_TG

Cuts at:      0    1680    2499 
   Size:       1680     819
 
NsiI A_TGCA'T

Cuts at:      0       5    2499 
   Size:          5    2494
 
NspI r_CATG'y

Cuts at:      0     235     670     898    2499 
   Size:        235     435     228    1601

  Fragments arranged by size: 

                1601     435     235     228
 
PpuMI rG'GwC_Cy

Cuts at:      0      54    2499 
   Size:         54    2445
 
PspOMI G'GGCC_C

Cuts at:      0    1026    2499 
   Size:       1026    1473
 
PspXI vC'TCGA_Gb

Cuts at:      0     691    2499 
   Size:        691    1808
 
PvuII CAG'CTG

Cuts at:      0     627    1506    2499 
   Size:        627     879     993

  Fragments arranged by size: 

                 993     879     627
 
SacI G_AGCT'C

Cuts at:      0    1932    2499 
   Size:       1932     567
 
SexAI A'CCwGG_T

Cuts at:      0     932    2018    2499 
   Size:        932    1086     481

  Fragments arranged by size: 

                1086     932     481
 
SfcI C'TryA_G

Cuts at:      0    1619    2499 
   Size:       1619     880
 
SmaI CCC'GGG

Cuts at:      0    2356    2499 
   Size:       2356     143
 
SmlI C'TyrA_G

Cuts at:      0     691    2499 
   Size:        691    1808
 
SphI G_CATG'C

Cuts at:      0     235    2499 
   Size:        235    2264
 
SspI AAT'ATT

Cuts at:      0    1536    2499 
   Size:       1536     963
 
StyI C'CwwG_G

Cuts at:      0     473     525    1109    1458    1685    1843    2402    2499 
   Size:        473      52     584     349     227     158     559      97

  Fragments arranged by size: 

                 584     559     473     349     227     158      97      52
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     229    2499 
   Size:        229    2270
 
TatI w'GTAC_w

Cuts at:      0    2304    2499 
   Size:       2304     195
 
Tth111I GACn'n_nGTC

Cuts at:      0     216    2499 
   Size:        216    2283
 
XhoI C'TCGA_G

Cuts at:      0     691    2499 
   Size:        691    1808
 
XmaI C'CCGG_G

Cuts at:      0    2354    2499 
   Size:       2354     145
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI    AlwNI     ApaI     ApoI     AseI
     AvaI    BamHI    BanII     BbsI    BbvCI     BclI    BfrBI    BglII
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaBI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BssSI   BstAPI    BstBI    BstYI     BtgI     BtsI
    CspCI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI    Hin4I  HindIII     MscI     MslI   MspA1I     NcoI     NdeI
     NsiI     NspI    PpuMI   PspOMI    PspXI    PvuII     SacI    SexAI
     SfcI     SmaI     SmlI     SphI     SspI     StyI    TaqII     TatI
  Tth111I     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI    ApaLI     AscI    AsiSI    AvrII     BaeI     BanI
     BbeI     BcgI    BciVI     BglI     BlpI     BmtI     BplI    BpuEI
    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BsrFI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
     DraI   DraIII     DrdI     EagI     EciI    EcoNI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     StuI     SwaI     TstI     XbaI     XcmI     XmnI
     ZraI
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