Restriction map for pF1KA1677
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AviII       AvrII       BanI        BbeI        
BbrPI       BbvCI       BciVI       BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       BstAPI      Bsu36I      
CelII       CfoI        ClaI        CpoI        DraIII      
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      KasI        
KpnI        KspI        MfeI        MluI        MslI        
MspA1I      MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         1540  GTATAC               GT^[AC][GT]AC
AccII        1428  CGCG                 CG^CG
AhdI          508  GACTTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1550  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1011  GGGCCC               GGGCC^C
AspEI         508  GACTTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1059  CCCGAG               C^[CT]CG[GA]G
BamHI        1227  GGATCC               G^GATCC
BanII        1011  GGGCCC               G[GA]GC[CT]^C
BcgI          952  CGACCTGACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         1014  CCCGG                CC^[GC]GG
BglII         380  AGATCT               A^GATCT
BpmI          633  CTGGAG               CTGGAG(16/14)
BseRI          76  GAGGAG               GAGGAG(10/8)
BsiYI        1394  CCAACTGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1394  CCAACTGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI        1059  CCCGAG               C^[CT]CG[GA]G
BspHI         303  TCATGA               T^CATGA
Bst1107I     1540  GTATAC               GTA^TAC
BstBI        1510  TTCGAA               TT^CGAA
BstEII       1389  GGTTACC              G^GT[ACGT]ACC
BstPI        1389  GGTTACC              G^GT[ACGT]ACC
BstUI        1428  CGCG                 CG^CG
BstXI         910  CCAAGAGTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1540  GTATAC               GTA^TAC
DraI          219  TTTAAA               TTT^AAA
DraII        1501  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DrdI           40  GACACCAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1602  CCATGG               C^C[GA][CT]GG
Eam1105I      508  GACTTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1003  GAAGAG               CTCTTC(1/4)
EcoO109I     1501  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1389  GGTTACC              G^GT[ACGT]ACC
EcoRI        1024  GAATTC               G^AATTC
EcoT14I      1602  CCATGG               C^C[AT][AT]GG
FauI          998  GCGGG                CCCGC(4/6)
HgaI          629  GACGC                GACGC(5/10)
HindIII      1465  AAGCTT               A^AGCTT
Ksp632I      1003  GAAGAG               CTCTTC(1/4)
MaeII          11  ACGT                 A^CGT
MvnI         1428  CGCG                 CG^CG
NciI         1014  CCCGG                CC^[GC]GG
NcoI         1602  CCATGG               C^CATGG
NspV         1510  TTCGAA               TT^CGAA
PflMI        1394  CCAACTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          303  TCATGA               T^CATGA
SapI         1003  GAAGAGC              GCTCTTC(1/4)
SfuI         1510  TTCGAA               TT^CGAA
SgrAI        1290  CACCGGTG             C[GA]^CCGG[CT]G
StyI         1602  CCATGG               C^C[AT][AT]GG
Van91I       1394  CCAACTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          910  CCAAGAGTCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII       1692  TCCGGA               T^CCGGA
             1776  TCCGGA              
AgeI          238  ACCGGT               A^CCGGT
             1291  ACCGGT              
AspHI          65  GAGCAC               G[AT]GC[AT]^C
             1267  GTGCTC              
BalI          146  TGGCCA               TGG^CCA
             1599  TGGCCA              
BbsI          626  GAAGAC               GAAGAC(2/6)
             1474  GTCTTC              
BclI          558  TGATCA               T^GATCA
             1557  TGATCA              
BpuAI         626  GAAGAC               GAAGAC(2/6)
             1474  GTCTTC              
BsaBI         553  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1228  GATCCTTATC          
BsaJI        1602  CCATGG               C^C[ACGT][ACGT]GG
             1726  CCTCGG              
BseAI        1692  TCCGGA               T^CCGGA
             1776  TCCGGA              
BsgI          398  GTGCAG               GTGCAG(16/14)
              601  GTGCAG              
BsiHKAI        65  GAGCAC               G[AT]GC[AT]^C
             1267  GTGCTC              
BspEI        1692  TCCGGA               T^CCGGA
             1776  TCCGGA              
BspMI        1766  ACCTGC               ACCTGC(4/8)
              569  GCAGGT              
BsrFI         238  ACCGGT               [GA]^CCGG[CT]
             1291  ACCGGT              
BstYI         380  AGATCT               [GA]^GATC[CT]
             1227  GGATCC              
Cfr10I        238  ACCGGT               [GA]^CCGG[CT]
             1291  ACCGGT              
EaeI          146  TGGCCA               [CT]^GGCC[GA]
             1599  TGGCCA              
FbaI          558  TGATCA               T^GATCA
             1557  TGATCA              
HpaI          880  GTTAAC               GTT^AAC
             1240  GTTAAC              
MamI          553  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1228  GATCCTTATC          
MflI          380  AGATCT               [GA]^GATC[CT]
             1227  GGATCC              
MluNI         146  TGGCCA               TGG^CCA
             1599  TGGCCA              
MroI         1692  TCCGGA               T^CCGGA
             1776  TCCGGA              
MscI          146  TGGCCA               TGG^CCA
             1599  TGGCCA              
PinAI         238  ACCGGT               A^CCGGT
             1291  ACCGGT              
PstI          182  CTGCAG               CTGCA^G
             1078  CTGCAG              
SmlI         1382  CTCAAG               C^T[CT][GA]AG
             1497  CTTGAG              
StuI          502  AGGCCT               AGG^CCT
             1644  AGGCCT              
Tsp45I         97  GTCAC                ^GT[GC]AC
             1609  GTGAC               
XhoII         380  AGATCT               [GA]^GATC[CT]
             1227  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbvI        BfaI        BmyI        
BsaWI       BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     
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