Restriction map for pF1KA1625
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# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AviII       AvrII       
BanI        BbeI        BbvCI       BcgI        BfrI        
BlnI        BsaBI       BsgI        BsiWI       BsmI        
Bsp1407I    BspDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FseI        FspI        
HaeII       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MunI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfoI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        XbaI        XcmI        XhoI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccIII       1428  TCCGGA               T^CCGGA
AcyI          666  GACGCC               G[GA]^CG[CT]C
AflIII       1412  ACATGT               A^C[GA][CT]GT
AgeI          556  ACCGGT               A^CCGGT
ApaI         2107  GGGCCC               GGGCC^C
AspHI        1837  GAGCTC               G[AT]GC[AT]^C
AspI         2043  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI         780  CACGTG               CAC^GTG
BbsI          490  GAAGAC               GAAGAC(2/6)
BclI         1889  TGATCA               T^GATCA
BglI          501  GCCTGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          513  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      513  GCTCAGC              GC^T[ACGT]AGC
BpuAI         490  GAAGAC               GAAGAC(2/6)
BsaAI         780  CACGTG               [CT]AC^GT[GA]
BsaHI         666  GACGCC               G[GA]^CG[CT]C
BseAI        1428  TCCGGA               T^CCGGA
BsiEI        1258  CGACCG               CG[GA][CT]^CG
BsiHKAI      1837  GAGCTC               G[AT]GC[AT]^C
BsmBI        1999  GAGACG               CGTCTC(1/5)
BspEI        1428  TCCGGA               T^CCGGA
BspHI        1025  TCATGA               T^CATGA
BspLU11I     1412  ACATGT               A^CATGT
BsrDI        1192  CATTGC               GCAATG(2/0)
BssSI         416  CACGAG               CACGAG(-5/-1)
BstBI         635  TTCGAA               TT^CGAA
CelII         513  GCTCAGC              GC^T[ACGT]AGC
CfoI         2099  GCGC                 GCG^C
EarI         1384  CTCTTC               CTCTTC(1/4)
EcoNI        2154  CCTTCCGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         850  GAATTC               G^AATTC
FbaI         1889  TGATCA               T^GATCA
HhaI         2099  GCGC                 GCG^C
Hin1I         666  GACGCC               G[GA]^CG[CT]C
HinP1I       2099  GCGC                 G^CGC
Ksp632I      1384  CTCTTC               CTCTTC(1/4)
MroI         1428  TCCGGA               T^CCGGA
NcoI         1503  CCATGG               C^CATGG
NdeI         2198  CATATG               CA^TATG
NspI         1412  ACATGT               [GA]CATG^[CT]
NspV          635  TTCGAA               TT^CGAA
PflFI        2043  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         556  ACCGGT               A^CCGGT
PmaCI         780  CACGTG               CAC^GTG
PmlI          780  CACGTG               CAC^GTG
RcaI         1025  TCATGA               T^CATGA
SacI         1837  GAGCTC               GAGCT^C
SapI         1383  GCTCTTC              GCTCTTC(1/4)
SfiI          500  GGCCTGGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          635  TTCGAA               TT^CGAA
SgrAI         555  CACCGGTG             C[GA]^CCGG[CT]G
SmaI           10  CCCGGG               CCC^GGG
SmlI         1045  CTCAAG               C^T[CT][GA]AG
SspI         1405  AATATT               AAT^ATT
Tth111I      2043  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI          10  CCCGGG               C^CCGGG
XmaI           10  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI          947  GTAC                 GT^AC
             1648  GTAC                
AhdI         1450  GACCACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1924  GACTGGAAGTC         
AspEI        1450  GACCACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1924  GACTGGAAGTC         
AvaI           10  CCCGGG               C^[CT]CG[GA]G
              810  CTCGGG              
BalI          390  TGGCCA               TGG^CCA
             1328  TGGCCA              
BamHI        1622  GGATCC               G^GATCC
             1691  GGATCC              
BciVI         334  GGATAC               GTATCC(6/5)
              797  GGATAC              
BfaI         1132  CTAG                 C^TAG
             1627  CTAG                
BglII        1128  AGATCT               A^GATCT
             1578  AGATCT              
BsaI          884  GAGACC               GGTCTC(1/5)
             2248  GAGACC              
BsoBI          10  CCCGGG               C^[CT]CG[GA]G
              810  CTCGGG              
BsrBI         511  CCGCTC               CCGCTC(-3/-3)
             2062  CCGCTC              
BsrFI         433  ACCGGC               [GA]^CCGG[CT]
              556  ACCGGT              
Cfr10I        433  ACCGGC               [GA]^CCGG[CT]
              556  ACCGGT              
DraII        2106  AGGGCCC              [GA]G^G[ACGT]CC[CT]
             2107  GGGCCCC             
DraIII       1468  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1951  CACTGTGTG           
Eam1105I     1450  GACCACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1924  GACTGGAAGTC         
EcoO109I     2106  AGGGCCC              [GA]G^G[ACGT]CC[CT]
             2107  GGGCCCC             
FauI         2061  CCCGC                CCCGC(4/6)
             2255  GCGGG               
HgaI          666  GACGC                GACGC(5/10)
             2137  GACGC               
MaeI         1132  CTAG                 C^TAG
             1627  CTAG                
MluNI         390  TGGCCA               TGG^CCA
             1328  TGGCCA              
MscI          390  TGGCCA               TGG^CCA
             1328  TGGCCA              
MslI          231  CATTAGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1200  CATCGAAGTG          
PvuII         516  CAGCTG               CAG^CTG
             1549  CAGCTG              
RsaI          947  GTAC                 GT^AC
             1648  GTAC                
Tsp45I       1735  GTCAC                ^GT[GC]AC
             2041  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AcsI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AvaII       BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BsaWI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PstI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI        
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