Restriction map for pF1KA1625
 (Linear) (Six-base) MAPSORT of: KIAA1625.seq  Check: 2071  from: 1  to: 2271



KIAA1625.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     604    1156    1879    1966    2271 
   Size:         75     529     552     723      87     305

  Fragments arranged by size: 

                 723     552     529     305      87      75
 
AflIII A'CryG_T

Cuts at:      0    1412    2271 
   Size:       1412     859
 
AgeI A'CCGG_T

Cuts at:      0     556    2271 
   Size:        556    1715
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1455    1929    2271 
   Size:       1455     474     342

  Fragments arranged by size: 

                1455     474     342
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1744    1990    2271 
   Size:       1744     246     281

  Fragments arranged by size: 

                1744     281     246
 
AlwNI CAG_nnn'CTG

Cuts at:      0     376     955    1065    1143    1554    2271 
   Size:        376     579     110      78     411     717

  Fragments arranged by size: 

                 717     579     411     376     110      78
 
ApaI G_GGCC'C

Cuts at:      0    2111    2271 
   Size:       2111     160
 
ApoI r'AATT_y

Cuts at:      0     254     850    1291    2271 
   Size:        254     596     441     980

  Fragments arranged by size: 

                 980     596     441     254
 
AvaI C'yCGr_G

Cuts at:      0      10     810    2271 
   Size:         10     800    1461

  Fragments arranged by size: 

                1461     800      10
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     327     360    2271 
   Size:        327      33    1911

  Fragments arranged by size: 

                1911     327      33
 
BamHI G'GATC_C

Cuts at:      0    1622    1691    2271 
   Size:       1622      69     580

  Fragments arranged by size: 

                1622     580      69
 
BanII G_rGCy'C

Cuts at:      0     881    1055    1087    1385    1841    2111    2271 
   Size:        881     174      32     298     456     270     160

  Fragments arranged by size: 

                 881     456     298     270     174     160      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     497    2271 
   Size:        497    1774
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     328     791    2271 
   Size:        328     463    1480

  Fragments arranged by size: 

                1480     463     328
 
BclI T'GATC_A

Cuts at:      0    1889    2271 
   Size:       1889     382
 
BglI GCCn_nnn'nGGC

Cuts at:      0     507    2271 
   Size:        507    1764
 
BglII A'GATC_T

Cuts at:      0    1128    1578    2271 
   Size:       1128     450     693

  Fragments arranged by size: 

                1128     693     450
 
BlpI GC'TnA_GC

Cuts at:      0     514    2271 
   Size:        514    1757
 
Bme1580I G_kGCm'C

Cuts at:      0    1538    2111    2271 
   Size:       1538     573     160

  Fragments arranged by size: 

                1538     573     160
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     990    1070    1800    2054    2271 
   Size:        990      80     730     254     217

  Fragments arranged by size: 

                 990     730     254     217      80
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2080    2271 
   Size:       2080     191
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15     590     791    1111    1800    1903    2271 
   Size:         15     575     201     320     689     103     368

  Fragments arranged by size: 

                 689     575     368     320     201     103      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1030    2271 
   Size:       1030    1241
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     878    2242    2271 
   Size:        878    1364      29

  Fragments arranged by size: 

                1364     878      29
 
BsaAI yAC'GTr

Cuts at:      0     782    2271 
   Size:        782    1489
 
BsaHI Gr'CG_yC

Cuts at:      0     667    2271 
   Size:        667    1604
 
BsaWI w'CCGG_w

Cuts at:      0     331     556    1428    1763    2180    2271 
   Size:        331     225     872     335     417      91

  Fragments arranged by size: 

                 872     417     335     331     225      91
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     587    1114    1243    1756    2260    2271 
   Size:        587     527     129     513     504      11

  Fragments arranged by size: 

                 587     527     513     504     129      11
 
BseYI C'CCAG_C

Cuts at:      0     932    1054    1554    2271 
   Size:        932     122     500     717

  Fragments arranged by size: 

                 932     717     500     122
 
BsiEI CG_ry'CG

Cuts at:      0    1261    2271 
   Size:       1261    1010
 
BsiHKAI G_wGCw'C

Cuts at:      0    1841    2271 
   Size:       1841     430
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1993    2271 
   Size:       1993     278
 
Bsp1286I G_dGCh'C

Cuts at:      0     881    1055    1087    1385    1538    1841    2111    2271 
   Size:        881     174      32     298     153     303     270     160

  Fragments arranged by size: 

                 881     303     298     270     174     160     153      32
            
 
BspEI T'CCGG_A

Cuts at:      0    1428    2271 
   Size:       1428     843
 
BspHI T'CATG_A

Cuts at:      0    1025    2271 
   Size:       1025    1246
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     575    2179    2260    2271 
   Size:        575    1604      81      11

  Fragments arranged by size: 

                1604     575      81      11
 
BsrBI CCG'CTC

Cuts at:      0     513    2064    2271 
   Size:        513    1551     207

  Fragments arranged by size: 

                1551     513     207
 
BsrDI GCAATG_nn'

Cuts at:      0    1191    2271 
   Size:       1191    1080
 
BsrFI r'CCGG_y

Cuts at:      0     433     556    2271 
   Size:        433     123    1715

  Fragments arranged by size: 

                1715     433     123
 
BssSI C'ACGA_G

Cuts at:      0     416    2271 
   Size:        416    1855
 
BstBI TT'CG_AA

Cuts at:      0     636    2271 
   Size:        636    1635
 
BstYI r'GATC_y

Cuts at:      0      41     348    1128    1578    1622    1691    2271 
   Size:         41     307     780     450      44      69     580

  Fragments arranged by size: 

                 780     580     450     307      69      44      41
 
BtgI C'CryG_G

Cuts at:      0    1296    1503    2240    2271 
   Size:       1296     207     737      31

  Fragments arranged by size: 

                1296     737     207      31
 
BtsI GCAGTG_nn'

Cuts at:      0     145     919    1069    2271 
   Size:        145     774     150    1202

  Fragments arranged by size: 

                1202     774     150     145
 
DraIII CAC_nnn'GTG

Cuts at:      0    1473    1956    2271 
   Size:       1473     483     315

  Fragments arranged by size: 

                1473     483     315
 
EaeI y'GGCC_r

Cuts at:      0     390     687    1328    1958    2271 
   Size:        390     297     641     630     313

  Fragments arranged by size: 

                 641     630     390     313     297
 
EarI CTCTTCn'nnn_

Cuts at:      0    1390    2271 
   Size:       1390     881
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1494    1704    2271 
   Size:       1494     210     567

  Fragments arranged by size: 

                1494     567     210
 
EcoICRI GAG'CTC

Cuts at:      0    1839    2271 
   Size:       1839     432
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15      75     590     604     791    1111    1156    1800 
   Size:         15      60     515      14     187     320      45     644

Cuts at:   1800    1879    1903    1966    2271 
   Size:         79      24      63     305

  Fragments arranged by size: 

                 644     515     320     305     187      79      63      60
                  45      24      15      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2158    2271 
   Size:       2158     113
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2107    2108    2271 
   Size:       2107       1     163

  Fragments arranged by size: 

                2107     163       1
 
EcoRI G'AATT_C

Cuts at:      0     850    2271 
   Size:        850    1421
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     102    2271 
   Size:        102    2169
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     789     821    1118    1150    1205    1237    1441    1473 
   Size:        789      32     297      32      55      32     204      32

Cuts at:   1473    1499    1531    1570    1602    1915    1947    2271 
   Size:         26      32      39      32     313      32     324

  Fragments arranged by size: 

                 789     324     313     297     204      55      39      32
                  32      32      32      32      32      32      26
 
MscI TGG'CCA

Cuts at:      0     392    1330    2271 
   Size:        392     938     941

  Fragments arranged by size: 

                 941     938     392
 
MslI CAynn'nnrTG

Cuts at:      0     235    1204    2271 
   Size:        235     969    1067

  Fragments arranged by size: 

                1067     969     235
 
MspA1I CmG'CkG

Cuts at:      0     518     630    1065    1551    2271 
   Size:        518     112     435     486     720

  Fragments arranged by size: 

                 720     518     486     435     112
 
NcoI C'CATG_G

Cuts at:      0    1503    2271 
   Size:       1503     768
 
NdeI CA'TA_TG

Cuts at:      0    2199    2271 
   Size:       2199      72
 
NspI r_CATG'y

Cuts at:      0    1416    2271 
   Size:       1416     855
 
PciI A'CATG_T

Cuts at:      0    1412    2271 
   Size:       1412     859
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     345     399    1337    1473    2271 
   Size:        345      54     938     136     798

  Fragments arranged by size: 

                 938     798     345     136      54
 
PmlI CAC'GTG

Cuts at:      0     782    2271 
   Size:        782    1489
 
PspOMI G'GGCC_C

Cuts at:      0    2107    2271 
   Size:       2107     164
 
PstI C_TGCA'G

Cuts at:      0     457    1700    1823    2036    2271 
   Size:        457    1243     123     213     235

  Fragments arranged by size: 

                1243     457     235     213     123
 
PvuII CAG'CTG

Cuts at:      0     518    1551    2271 
   Size:        518    1033     720

  Fragments arranged by size: 

                1033     720     518
 
SacI G_AGCT'C

Cuts at:      0    1841    2271 
   Size:       1841     430
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1390    2271 
   Size:       1390     881
 
SfcI C'TryA_G

Cuts at:      0     453     661    1696    1819    2032    2093    2271 
   Size:        453     208    1035     123     213      61     178

  Fragments arranged by size: 

                1035     453     213     208     178     123      61
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     507    2271 
   Size:        507    1764
 
SgrAI Cr'CCGG_yG

Cuts at:      0     556    2271 
   Size:        556    1715
 
SmaI CCC'GGG

Cuts at:      0      12    2271 
   Size:         12    2259
 
SmlI C'TyrA_G

Cuts at:      0    1045    2271 
   Size:       1045    1226
 
SspI AAT'ATT

Cuts at:      0    1407    2271 
   Size:       1407     864
 
StyI C'CwwG_G

Cuts at:      0    1009    1503    2067    2271 
   Size:       1009     494     564     204

  Fragments arranged by size: 

                1009     564     494     204
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1275    2271 
   Size:       1275     996
 
TatI w'GTAC_w

Cuts at:      0     946    1647    2271 
   Size:        946     701     624

  Fragments arranged by size: 

                 946     701     624
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     166     198    2271 
   Size:        166      32    2073

  Fragments arranged by size: 

                2073     166      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2046    2271 
   Size:       2046     225
 
XmaI C'CCGG_G

Cuts at:      0      10    2271 
   Size:         10    2261
 
XmnI GAAnn'nnTTC

Cuts at:      0      85     854    1828    2271 
   Size:         85     769     974     443

  Fragments arranged by size: 

                 974     769     443      85
 

 Enzymes that do cut:

     AcuI   AflIII     AgeI     AhdI     AlfI    AlwNI     ApaI     ApoI
     AvaI     BaeI    BamHI    BanII     BbsI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
    BstBI    BstYI     BtgI     BtsI   DraIII     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I     MscI
     MslI   MspA1I     NcoI     NdeI     NspI     PciI    PflMI     PmlI
   PspOMI     PstI    PvuII     SacI     SapI     SfcI     SfiI    SgrAI
     SmaI     SmlI     SspI     StyI    TaqII     TatI     TstI  Tth111I
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AleI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BanI     BbeI
    BbvCI     BcgI    BfrBI    BmgBI     BmtI   Bpu10I    BsaBI    BsaXI
     BsgI    BsiWI     BsmI    BsrGI   BssHII   BstAPI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfoI     SgfI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     XbaI     XcmI
     XhoI     ZraI
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