Restriction map for pF1KA1342
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BanI        BanII       BbeI        BbvCI       
BcgI        BclI        BglI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MscI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII        958  TCCGGA               T^CCGGA
AfaI          993  GTAC                 GT^AC
AflII         169  CTTAAG               C^TTAAG
AvaI          783  CTCGGG               C^[CT]CG[GA]G
AvaII        1145  GGTCC                G^G[AT]CC
BamHI         645  GGATCC               G^GATCC
BbrPI        1258  CACGTG               CAC^GTG
BciVI         680  GGATAC               GTATCC(6/5)
BcnI           21  CCGGG                CC^[GC]GG
BfaI          623  CTAG                 C^TAG
BfrI          169  CTTAAG               C^TTAAG
BpmI          352  CTGGAG               CTGGAG(16/14)
BsaAI        1258  CACGTG               [CT]AC^GT[GA]
BsaI          661  GAGACC               GGTCTC(1/5)
BsaWI         958  TCCGGA               [AT]^CCGG[AT]
BseAI         958  TCCGGA               T^CCGGA
BseRI         143  CTCCTC               GAGGAG(10/8)
BsgI         1181  GTGCAG               GTGCAG(16/14)
BsmFI        1146  GTCCC                GGGAC(10/14)
BsmI          357  GAATGC               GAATGC(1/-1)
BsoBI         783  CTCGGG               C^[CT]CG[GA]G
BspEI         958  TCCGGA               T^CCGGA
BsrDI         272  CATTGC               GCAATG(2/0)
BstXI          44  CCACAGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          414  TTTAAA               TTT^AAA
EcoRI         267  GAATTC               G^AATTC
EcoRV         754  GATATC               GAT^ATC
MaeI          623  CTAG                 C^TAG
MaeII        1259  ACGT                 A^CGT
MroI          958  TCCGGA               T^CCGGA
MslI           45  CACAGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        254  CAGCTG               C[AC]G^C[GT]G
MwoI          252  GCCAGCTGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI           21  CCGGG                CC^[GC]GG
NcoI          545  CCATGG               C^CATGG
NspI          162  GCATGT               [GA]CATG^[CT]
PflMI         699  CCAAGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1258  CACGTG               CAC^GTG
PmlI         1258  CACGTG               CAC^GTG
PpuMI        1144  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         254  CAGCTG               CAG^CTG
RsaI          993  GTAC                 GT^AC
SfaNI         276  GCATC                GCATC(5/9)
SmlI          169  CTTAAG               C^T[CT][GA]AG
Tsp45I       1226  GTGAC                ^GT[GC]AC
Van91I        699  CCAAGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AspHI        1207  GAGCAC               G[AT]GC[AT]^C
             1261  GTGCTC              
BbvI         1183  GCAGC                GCAGC(8/12)
             1186  GCAGC               
BglII         297  AGATCT               A^GATCT
             1220  AGATCT              
BmyI         1207  GAGCAC               G[AGT]GC[ACT]^C
             1261  GTGCTC              
BsiHKAI      1207  GAGCAC               G[AT]GC[AT]^C
             1261  GTGCTC              
Bsp1286I     1207  GAGCAC               G[AGT]GC[ACT]^C
             1261  GTGCTC              
BsrI         1198  ACTGG                ACTGG(1/-1)
             1211  ACTGG               
BstF5I        125  CATCC                GGATG(2/0)
              549  GGATG               
Cfr13I       1095  GGGCC                G^G[ACGT]CC
             1145  GGTCC               
DraII        1094  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1144  GGGTCCC             
DsaI          531  CCGTGG               C^C[GA][CT]GG
              545  CCATGG              
EarI          469  CTCTTC               CTCTTC(1/4)
              442  GAAGAG              
EcoO109I     1094  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1144  GGGTCCC             
EcoT14I       545  CCATGG               C^C[AT][AT]GG
              641  CCTTGG              
Fnu4HI       1183  GCAGC                GC^[ACGT]GC
             1186  GCAGC               
FokI          125  CATCC                GGATG(9/13)
              549  GGATG               
HapII          21  CCGG                 C^CGG
              959  CCGG                
HpaII          21  CCGG                 C^CGG
              959  CCGG                
HphI          865  GGTGA                GGTGA(8/7)
               11  TCACC               
ItaI         1183  GCAGC                GC^[ACGT]GC
             1186  GCAGC               
Ksp632I       469  CTCTTC               CTCTTC(1/4)
              442  GAAGAG              
MaeIII        870  GTTAC                ^GT[ACGT]AC
             1226  GTGAC               
MspI           21  CCGG                 C^CGG
              959  CCGG                
Sau96I       1095  GGGCC                G^G[ACGT]CC
             1145  GGTCC               
StyI          545  CCATGG               C^C[AT][AT]GG
              641  CCTTGG              
TfiI         1010  GAATC                G^A[AT]TC
             1132  GATTC               
TseI         1183  GCAGC                G^C[AT]GC
             1186  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BbsI        
BpuAI       BsaJI       BsiYI       BslI        BsmAI       
BssKI       BstNI       BstYI       Cac8I       DdeI        
DpnII       EcoRII      HaeIII      HinfI       MboI        
MboII       MflI        MnlI        MseI        MvaI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
ScrFI       TaqI        Tru9I       Tsp509I     TspRI       
TthHB8I     XhoII       
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