Restriction map for pF1KA1342
 (Linear) (Six-base) MAPSORT of: KIAA1342.seq  Check: 7896  from: 1  to: 1275



KIAA1342.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      45     422     821     839    1275 
   Size:         45     377     399      18     436

  Fragments arranged by size: 

                 436     399     377      45      18
 
AflII C'TTAA_G

Cuts at:      0     169    1275 
   Size:        169    1106
 
AleI CACnn'nnGTG

Cuts at:      0      49    1275 
   Size:         49    1226
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     123    1275 
   Size:        123    1152
 
ApoI r'AATT_y

Cuts at:      0      28     267    1117    1275 
   Size:         28     239     850     158

  Fragments arranged by size: 

                 850     239     158      28
 
AvaI C'yCGr_G

Cuts at:      0     783    1275 
   Size:        783     492
 
BamHI G'GATC_C

Cuts at:      0     645    1275 
   Size:        645     630
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      72     845    1030    1275 
   Size:         72     773     185     245

  Fragments arranged by size: 

                 773     245     185      72
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     674    1275 
   Size:        674     601
 
BglII A'GATC_T

Cuts at:      0     297    1220    1275 
   Size:        297     923      55

  Fragments arranged by size: 

                 923     297      55
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373    1275 
   Size:        373     902
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     655    1275 
   Size:        655     620
 
BsaAI yAC'GTr

Cuts at:      0    1260    1275 
   Size:       1260      15
 
BsaWI w'CCGG_w

Cuts at:      0     958    1275 
   Size:        958     317
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     134    1275 
   Size:        134    1141
 
BseYI C'CCAG_C

Cuts at:      0     459    1275 
   Size:        459     816
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1202    1275 
   Size:       1202      73
 
BsiHKAI G_wGCw'C

Cuts at:      0    1211    1265    1275 
   Size:       1211      54      10

  Fragments arranged by size: 

                1211      54      10
 
BsmI GAATG_Cn'

Cuts at:      0     363    1275 
   Size:        363     912
 
Bsp1286I G_dGCh'C

Cuts at:      0    1211    1265    1275 
   Size:       1211      54      10

  Fragments arranged by size: 

                1211      54      10
 
BspEI T'CCGG_A

Cuts at:      0     958    1275 
   Size:        958     317
 
BsrDI GCAATG_nn'

Cuts at:      0     271    1275 
   Size:        271    1004
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      51    1275 
   Size:         51    1224
 
BstYI r'GATC_y

Cuts at:      0      56     104     282     297     645     969    1220    1275 
   Size:         56      48     178      15     348     324     251      55

  Fragments arranged by size: 

                 348     324     251     178      56      55      48      15
            
 
BtgI C'CryG_G

Cuts at:      0     531     545    1275 
   Size:        531      14     730

  Fragments arranged by size: 

                 730     531      14
 
BtsI GCAGTG_nn'

Cuts at:      0    1061    1275 
   Size:       1061     214
 
DraI TTT'AAA

Cuts at:      0     416    1275 
   Size:        416     859
 
EarI CTCTTCn'nnn_

Cuts at:      0     437     475    1275 
   Size:        437      38     800

  Fragments arranged by size: 

                 800     437      38
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      45     373     422     821     839    1275 
   Size:         45     328      49     399      18     436

  Fragments arranged by size: 

                 436     399     328      49      45      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1095    1145    1275 
   Size:       1095      50     130

  Fragments arranged by size: 

                1095     130      50
 
EcoRI G'AATT_C

Cuts at:      0     267    1275 
   Size:        267    1008
 
EcoRV GAT'ATC

Cuts at:      0     756    1275 
   Size:        756     519
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      96     128     541     573     740     772     815     847 
   Size:         96      32     413      32     167      32      43      32

Cuts at:    847    1275 
   Size:        428

  Fragments arranged by size: 

                 428     413     167      96      43      32      32      32
                  32
 
MslI CAynn'nnrTG

Cuts at:      0      49    1275 
   Size:         49    1226
 
MspA1I CmG'CkG

Cuts at:      0     256    1275 
   Size:        256    1019
 
NcoI C'CATG_G

Cuts at:      0     545    1275 
   Size:        545     730
 
NspI r_CATG'y

Cuts at:      0     166    1275 
   Size:        166    1109
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     705    1275 
   Size:        705     570
 
PmlI CAC'GTG

Cuts at:      0    1260    1275 
   Size:       1260      15
 
PpuMI rG'GwC_Cy

Cuts at:      0    1145    1275 
   Size:       1145     130
 
PvuII CAG'CTG

Cuts at:      0     256    1275 
   Size:        256    1019
 
SanDI GG'GwC_CC

Cuts at:      0    1145    1275 
   Size:       1145     130
 
SmlI C'TyrA_G

Cuts at:      0     169    1275 
   Size:        169    1106
 
StyI C'CwwG_G

Cuts at:      0     545     641    1275 
   Size:        545      96     634

  Fragments arranged by size: 

                 634     545      96
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     706    1168    1275 
   Size:        706     462     107

  Fragments arranged by size: 

                 706     462     107
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      48      80    1275 
   Size:         48      32    1195

  Fragments arranged by size: 

                1195      48      32
 

 Enzymes that do cut:

     AcuI    AflII     AleI     AlfI     ApoI     AvaI    BamHI     BbsI
    BciVI    BglII     BpmI     BsaI    BsaAI    BsaWI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BsrDI    BstXI    BstYI
     BtgI     BtsI     DraI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV
    Hin4I     MslI   MspA1I     NcoI     NspI    PflMI     PmlI    PpuMI
    PvuII    SanDI     SmlI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BcgI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI    BsmBI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com