Restriction map for pF1KA1265
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AocI        
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BbeI        BbrPI       BcgI        BcnI        BfrI        
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmFI       BsoBI       BspDI       BspEI       BspMI       
BsrBI       BsrFI       BssHII      BssSI       BstBI       
BstEII      BstPI       BstUI       Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       FauI        FseI        FspI        HincII      
HindII      HpaI        KasI        KpnI        KspI        
MluI        MroI        MvnI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspI        StuI        Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI         2197  GTATAC               GT^[AC][GT]AC
AciI          528  CCGC                 CCGC(-3/-1)
AcyI         1360  GACGCC               G[GA]^CG[CT]C
AflIII        942  ACATGT               A^C[GA][CT]GT
Aor51HI       102  AGCGCT               AGC^GCT
AseI          295  ATTAAT               AT^TAAT
AsnI          295  ATTAAT               AT^TAAT
AvaII         795  GGTCC                G^G[AT]CC
BalI          312  TGGCCA               TGG^CCA
BamHI        1972  GGATCC               G^GATCC
BanII        2053  GGGCTC               G[GA]GC[CT]^C
BbsI         2116  GTCTTC               GAAGAC(2/6)
BbvCI        1905  GCTGAGG              CCTCAGC(-5/-2)
BciVI         433  GTATCC               GTATCC(6/5)
BglI         2215  GCCATGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI         2053  GGGCTC               G[AGT]GC[ACT]^C
BpuAI        2116  GTCTTC               GAAGAC(2/6)
BsaHI        1360  GACGCC               G[GA]^CG[CT]C
BsaI          646  GAGACC               GGTCTC(1/5)
BsmBI        1358  GAGACG               CGTCTC(1/5)
Bsp1286I     2053  GGGCTC               G[AGT]GC[ACT]^C
BspLU11I      942  ACATGT               A^CATGT
Bst1107I     2197  GTATAC               GTA^TAC
BstXI        2438  CCATTATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2197  GTATAC               GTA^TAC
CfoI          103  GCGC                 GCG^C
DraI          402  TTTAAA               TTT^AAA
EaeI          312  TGGCCA               [CT]^GGCC[GA]
EarI         1798  GAAGAG               CTCTTC(1/4)
Eco47III      102  AGCGCT               AGC^GCT
EcoT22I       511  ATGCAT               ATGCA^T
HaeII         102  AGCGCT               [GA]GCGC^[CT]
HapII        1694  CCGG                 C^CGG
HgaI         1360  GACGC                GACGC(5/10)
HhaI          103  GCGC                 GCG^C
Hin1I        1360  GACGCC               G[GA]^CG[CT]C
HinP1I        103  GCGC                 G^CGC
HindIII      1630  AAGCTT               A^AGCTT
HpaII        1694  CCGG                 C^CGG
Ksp632I      1798  GAAGAG               CTCTTC(1/4)
MluNI         312  TGGCCA               TGG^CCA
MscI          312  TGGCCA               TGG^CCA
MspA1I       2249  CAGCTG               C[AC]G^C[GT]G
MspI         1694  CCGG                 C^CGG
NsiI          511  ATGCAT               ATGCA^T
PleI         1705  GACTC                GAGTC(4/5)
PshBI         295  ATTAAT               AT^TAAT
PstI         2300  CTGCAG               CTGCA^G
PvuII        2249  CAGCTG               CAG^CTG
ScaI         2101  AGTACT               AGT^ACT
SmlI         1681  CTCAAG               C^T[CT][GA]AG
SphI         2234  GCATGC               GCATG^C
SwaI          401  ATTTAAAT             ATTT^AAAT


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AlwNI        1661  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2294  CAGTCACTG           
Asp700I       100  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1628  GAAAGCTTTC          
BanI         1048  GGTGCC               G^G[CT][GA]CC
             1928  GGCACC              
BglII         885  AGATCT               A^GATCT
              978  AGATCT              
BseRI        2206  CTCCTC               GAGGAG(10/8)
             1801  GAGGAG              
BsgI         1871  CTGCAC               GTGCAG(16/14)
             2066  GTGCAG              
BsmI          387  GCATTC               GAATGC(1/-1)
             1938  GCATTC              
Bsp1407I      813  TGTACA               T^GTACA
              837  TGTACA              
BsrGI         813  TGTACA               T^GTACA
              837  TGTACA              
MfeI         2060  CAATTG               C^AATTG
             2192  CAATTG              
MunI         2060  CAATTG               C^AATTG
             2192  CAATTG              
NcoI           90  CCATGG               C^CATGG
             1953  CCATGG              
NdeI           10  CATATG               CA^TATG
              508  CATATG              
SexAI         744  ACCAGGT              A^CC[AT]GGT
             1178  ACCTGGT             
SspBI         813  TGTACA               T^GTACA
              837  TGTACA              
XmnI          100  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1628  GAAAGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BbvI        BclI        BfaI        BsaBI       BsaJI       
BsiYI       BslI        BsmAI       BspHI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DsaI        EcoRII      EcoT14I     FbaI        Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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