Restriction map for pF1KA1265
 (Linear) (Six-base) MAPSORT of: KIAA1265.seq  Check: 1792  from: 1  to: 2493



KIAA1265.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AccI GT'mk_AC

Cuts at:      0    2198    2493 
   Size:       2198     295
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119    1208    1739    2028    2392    2493 
   Size:        119    1089     531     289     364     101

  Fragments arranged by size: 

                1089     531     364     289     119     101
 
AfeI AGC'GCT

Cuts at:      0     104    2493 
   Size:        104    2389
 
AflIII A'CryG_T

Cuts at:      0     942    2493 
   Size:        942    1551
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1534    2493 
   Size:       1534     959
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1666    2299    2493 
   Size:       1666     633     194

  Fragments arranged by size: 

                1666     633     194
 
ApoI r'AATT_y

Cuts at:      0      22     148     405     726    1309    2412    2493 
   Size:         22     126     257     321     583    1103      81

  Fragments arranged by size: 

                1103     583     321     257     126      81      22
 
AseI AT'TA_AT

Cuts at:      0     296    2493 
   Size:        296    2197
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     416     449    2493 
   Size:        416      33    2044

  Fragments arranged by size: 

                2044     416      33
 
BamHI G'GATC_C

Cuts at:      0    1972    2493 
   Size:       1972     521
 
BanI G'GyrC_C

Cuts at:      0    1048    1928    2493 
   Size:       1048     880     565

  Fragments arranged by size: 

                1048     880     565
 
BanII G_rGCy'C

Cuts at:      0    2057    2493 
   Size:       2057     436
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2109    2493 
   Size:       2109     384
 
BbvCI CC'TCA_GC

Cuts at:      0    1906    2493 
   Size:       1906     587
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     444    2493 
   Size:        444    2049
 
BclI T'GATC_A

Cuts at:      0     564     765    1401    2018    2493 
   Size:        564     201     636     617     475

  Fragments arranged by size: 

                 636     617     564     475     201
 
BfrBI ATG'CAT

Cuts at:      0     513    2493 
   Size:        513    1980
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2221    2493 
   Size:       2221     272
 
BglII A'GATC_T

Cuts at:      0     885     978    2493 
   Size:        885      93    1515

  Fragments arranged by size: 

                1515     885      93
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     602    2493 
   Size:        602    1891
 
Bpu10I CC'TnA_GC

Cuts at:      0    1906    2493 
   Size:       1906     587
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1666    2493 
   Size:       1666     827
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     640    2493 
   Size:        640    1853
 
BsaBI GATnn'nnATC

Cuts at:      0     890     995    2017    2493 
   Size:        890     105    1022     476

  Fragments arranged by size: 

                1022     890     476     105
 
BsaHI Gr'CG_yC

Cuts at:      0    1361    2493 
   Size:       1361    1132
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     702     732    2493 
   Size:        702      30    1761

  Fragments arranged by size: 

                1761     702      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1816    2197    2493 
   Size:       1816     381     296

  Fragments arranged by size: 

                1816     381     296
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1856    2087    2493 
   Size:       1856     231     406

  Fragments arranged by size: 

                1856     406     231
 
BsmI GAATG_Cn'

Cuts at:      0     387    1938    2493 
   Size:        387    1551     555

  Fragments arranged by size: 

                1551     555     387
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1352    2493 
   Size:       1352    1141
 
Bsp1286I G_dGCh'C

Cuts at:      0    2057    2493 
   Size:       2057     436
 
BspHI T'CATG_A

Cuts at:      0     300     861    1002    1848    2124    2493 
   Size:        300     561     141     846     276     369

  Fragments arranged by size: 

                 846     561     369     300     276     141
 
BsrDI GCAATG_nn'

Cuts at:      0      60      69     636    2493 
   Size:         60       9     567    1857

  Fragments arranged by size: 

                1857     567      60       9
 
BsrGI T'GTAC_A

Cuts at:      0     813     837    2493 
   Size:        813      24    1656

  Fragments arranged by size: 

                1656     813      24
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      71    2065    2299    2493 
   Size:         71    1994     234     194

  Fragments arranged by size: 

                1994     234     194      71
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2445    2493 
   Size:       2445      48
 
BstYI r'GATC_y

Cuts at:      0     306     885     978    1972    2285    2493 
   Size:        306     579      93     994     313     208

  Fragments arranged by size: 

                 994     579     313     306     208      93
 
BstZ17I GTA'TAC

Cuts at:      0    2199    2493 
   Size:       2199     294
 
BtgI C'CryG_G

Cuts at:      0      90    1884    1953    2493 
   Size:         90    1794      69     540

  Fragments arranged by size: 

                1794     540      90      69
 
BtsI GCAGTG_nn'

Cuts at:      0    1536    2297    2493 
   Size:       1536     761     196

  Fragments arranged by size: 

                1536     761     196
 
DraI TTT'AAA

Cuts at:      0     404    2493 
   Size:        404    2089
 
EaeI y'GGCC_r

Cuts at:      0     312    2493 
   Size:        312    2181
 
EarI CTCTTCn'nnn_

Cuts at:      0    1793    2493 
   Size:       1793     700
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119    1208    1739    2028    2392    2493 
   Size:        119    1089     531     289     364     101

  Fragments arranged by size: 

                1089     531     364     289     119     101
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     928    1744    2493 
   Size:        928     816     749

  Fragments arranged by size: 

                 928     816     749
 
HaeII r_GCGC'y

Cuts at:      0     106    2493 
   Size:        106    2387
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     963     995    2493 
   Size:        963      32    1498

  Fragments arranged by size: 

                1498     963      32
 
HindIII A'AGCT_T

Cuts at:      0    1630    2493 
   Size:       1630     863
 
MfeI C'AATT_G

Cuts at:      0    2060    2192    2493 
   Size:       2060     132     301

  Fragments arranged by size: 

                2060     301     132
 
MscI TGG'CCA

Cuts at:      0     314    2493 
   Size:        314    2179
 
MslI CAynn'nnrTG

Cuts at:      0      74      89     320     455     512     833    1001    1415 
   Size:         74      15     231     135      57     321     168     414

Cuts at:   1415    1421    1427    1439    1445    1546    1829    1853    1952 
   Size:          6       6      12       6     101     283      24      99

Cuts at:   1952    2233    2357    2493 
   Size:        281     124     136

  Fragments arranged by size: 

                 414     321     283     281     231     168     136     135
                 124     101      99      74      57      24      15      12
                   6       6       6
 
MspA1I CmG'CkG

Cuts at:      0    2251    2493 
   Size:       2251     242
 
NcoI C'CATG_G

Cuts at:      0      90    1953    2493 
   Size:         90    1863     540

  Fragments arranged by size: 

                1863     540      90
 
NdeI CA'TA_TG

Cuts at:      0      11     509    2493 
   Size:         11     498    1984

  Fragments arranged by size: 

                1984     498      11
 
NsiI A_TGCA'T

Cuts at:      0     515    2493 
   Size:        515    1978
 
NspI r_CATG'y

Cuts at:      0     946    1420    2238    2310    2493 
   Size:        946     474     818      72     183

  Fragments arranged by size: 

                 946     818     474     183      72
 
PciI A'CATG_T

Cuts at:      0     942    2493 
   Size:        942    1551
 
PstI C_TGCA'G

Cuts at:      0    2304    2493 
   Size:       2304     189
 
PvuII CAG'CTG

Cuts at:      0    2251    2493 
   Size:       2251     242
 
ScaI AGT'ACT

Cuts at:      0    2103    2493 
   Size:       2103     390
 
SexAI A'CCwGG_T

Cuts at:      0     744    1178    2493 
   Size:        744     434    1315

  Fragments arranged by size: 

                1315     744     434
 
SfcI C'TryA_G

Cuts at:      0    1340    2108    2300    2493 
   Size:       1340     768     192     193

  Fragments arranged by size: 

                1340     768     193     192
 
SmlI C'TyrA_G

Cuts at:      0    1681    2493 
   Size:       1681     812
 
SphI G_CATG'C

Cuts at:      0    2238    2493 
   Size:       2238     255
 
StyI C'CwwG_G

Cuts at:      0      90     270    1296    1953    2324    2493 
   Size:         90     180    1026     657     371     169

  Fragments arranged by size: 

                1026     657     371     180     169      90
 
SwaI ATTT'AAAT

Cuts at:      0     404    2493 
   Size:        404    2089
 
TatI w'GTAC_w

Cuts at:      0     813     837    2101    2493 
   Size:        813      24    1264     392

  Fragments arranged by size: 

                1264     813     392      24
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     373     405    2493 
   Size:        373      32    2088

  Fragments arranged by size: 

                2088     373      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1390    1420    1667    2493 
   Size:       1390      30     247     826

  Fragments arranged by size: 

                1390     826     247      30
 
XmnI GAAnn'nnTTC

Cuts at:      0     104    1632    2493 
   Size:        104    1528     861

  Fragments arranged by size: 

                1528     861     104
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AlfI    AlwNI     ApoI     AseI
     BaeI    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII     BmrI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaXI    BseRI     BsgI     BsmI    BsmBI Bsp1286I    BspHI
    BsrDI    BsrGI   BstAPI    BstXI    BstYI  BstZ17I     BtgI     BtsI
     DraI     EaeI     EarI  Eco57MI     FalI    HaeII    Hin4I  HindIII
     MfeI     MscI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI
     PciI     PstI    PvuII     ScaI    SexAI     SfcI     SmlI     SphI
     StyI     SwaI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BbeI
     BcgI     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI    BsaAI
    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI    BspEI    BspMI    BsrBI
    BsrFI   BssHII    BssSI    BstBI   BstEII   Bsu36I    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MluI     MmeI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
    TaqII  Tth111I     XbaI     XhoI     XmaI     ZraI
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