Restriction map for pF1KA1248
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# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AflIII      AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspLU11I    BspMI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     CelII       CfoI        ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        HaeII       
HhaI        HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MscI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tth111I     XbaI        XhoI        XhoII       


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcyI           57  GACGCC               G[GA]^CG[CT]C
AgeI          983  ACCGGT               A^CCGGT
AlwNI         196  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1039  GGGCCC               GGGCC^C
AspHI         574  GAGCTC               G[AT]GC[AT]^C
AvaI         1044  CCCGGG               C^[CT]CG[GA]G
BanI          124  GGCACC               G^G[CT][GA]CC
BglI         1041  GCCCCCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          398  CTGGAG               CTGGAG(16/14)
BsaHI          57  GACGCC               G[GA]^CG[CT]C
BseRI          31  GAGGAG               GAGGAG(10/8)
BsiHKAI       574  GAGCTC               G[AT]GC[AT]^C
BsoBI        1044  CCCGGG               C^[CT]CG[GA]G
BspHI         756  TCATGA               T^CATGA
BsrBI         990  CCGCTC               CCGCTC(-3/-3)
BsrFI         983  ACCGGT               [GA]^CCGG[CT]
BstAPI         12  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        983  ACCGGT               [GA]^CCGG[CT]
DrdI          924  GACTCGCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1055  CCATGG               C^C[GA][CT]GG
EaeI           68  CGGCCA               [CT]^GGCC[GA]
Hin1I          57  GACGCC               G[GA]^CG[CT]C
MseI          428  TTAA                 T^TAA
MspA1I        844  CAGCTG               C[AC]G^C[GT]G
NcoI         1055  CCATGG               C^CATGG
PinAI         983  ACCGGT               A^CCGGT
PvuII         844  CAGCTG               CAG^CTG
RcaI          756  TCATGA               T^CATGA
SacI          574  GAGCTC               GAGCT^C
SapI          571  GAAGAGC              GCTCTTC(1/4)
ScaI          881  AGTACT               AGT^ACT
SmaI         1044  CCCGGG               CCC^GGG
SmlI          814  CTCAAG               C^T[CT][GA]AG
TaqI          212  TCGA                 T^CGA
Tru9I         428  TTAA                 T^TAA
Tsp45I        106  GTCAC                ^GT[GC]AC
TthHB8I       212  TCGA                 T^CGA
XcmI          482  CCAAGGGTTGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1044  CCCGGG               C^CCGGG
XmaI         1044  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccIII        537  TCCGGA               T^CCGGA
              829  TCCGGA              
AcsI          363  AAATTT               [GA]^AATT[CT]
              585  AAATTC              
AocI           61  CCTGAGG              CC^T[ACGT]AGG
              714  CCTCAGG             
ApoI          363  AAATTT               [GA]^AATT[CT]
              585  AAATTC              
Asp700I       226  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1025  GAACTCTTTC          
BseAI         537  TCCGGA               T^CCGGA
              829  TCCGGA              
BsgI           19  GTGCAG               GTGCAG(16/14)
              962  GTGCAG              
BspEI         537  TCCGGA               T^CCGGA
              829  TCCGGA              
Bsu36I         61  CCTGAGG              CC^T[ACGT]AGG
              714  CCTCAGG             
Cac8I         327  GCTTGC               GC[ACGT]^[ACGT]GC
              860  GCAAGC              
DraII        1038  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1039  GGGCCCC             
DraIII         79  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              163  CACGATGTG           
Eco81I         61  CCTGAGG              CC^T[ACGT]AGG
              714  CCTCAGG             
EcoO109I     1038  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1039  GGGCCCC             
EcoRV         302  GATATC               GAT^ATC
              706  GATATC              
FauI          989  CCCGC                CCCGC(4/6)
              246  GCGGG               
HgaI           57  GACGC                GACGC(5/10)
              347  GCGTC               
MaeIII        106  GTCAC                ^GT[ACGT]AC
              779  GTAAC               
MroI          537  TCCGGA               T^CCGGA
              829  TCCGGA              
MslI          253  CATGTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              757  CATGAAGATG          
NspI          218  ACATGC               [GA]CATG^[CT]
              624  ACATGC              
PflMI         162  CCACGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              482  CCAAGGGTTGG         
PstI           10  CTGCAG               CTGCA^G
              352  CTGCAG              
SfcI           10  CTGCAG               C^T[GA][CT]AG
              352  CTGCAG              
StuI          519  AGGCCT               AGG^CCT
              872  AGGCCT              
Van91I        162  CCACGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              482  CCAAGGGTTGG         
XmnI          226  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1025  GAACTCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanII       BbvI        BcnI        BmyI        BsaI        
BsaJI       BsaWI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MboI        MboII       MnlI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TseI        Tsp509I     
TspRI       
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