Restriction map for pF1KA1191
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BciVI       BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FauI        FseI        
FspI        HaeII       Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          227  GTCTAC               GT^[AC][GT]AC
AcsI          318  GAATTC               [GA]^AATT[CT]
AlwNI         564  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          863  GGGCCC               GGGCC^C
ApoI          318  GAATTC               [GA]^AATT[CT]
Asp700I       346  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         895  GTGCTC               G[AT]GC[AT]^C
AvaI          109  CTCGAG               C^[CT]CG[GA]G
BcgI          111  CGAGGACCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          326  TGATCA               T^GATCA
BcnI           84  CCGGG                CC^[GC]GG
BglI          718  GCCACCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         767  TACGTG               [CT]AC^GT[GA]
BsaBI         822  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          424  GAGACC               GGTCTC(1/5)
BsiHKAI       895  GTGCTC               G[AT]GC[AT]^C
BsmAI         424  GAGAC                GTCTC(1/5)
BsmI          350  GCATTC               GAATGC(1/-1)
BsoBI         109  CTCGAG               C^[CT]CG[GA]G
BspHI         314  TCATGA               T^CATGA
BstF5I        828  CATCC                GGATG(2/0)
BstXI         779  CCAACCGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI          443  TGGCCG               [CT]^GGCC[GA]
EcoNI         198  CCTCGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         318  GAATTC               G^AATTC
FbaI          326  TGATCA               T^GATCA
FokI          828  CATCC                GGATG(9/13)
HapII          84  CCGG                 C^CGG
HgaI          155  GCGTC                GACGC(5/10)
HpaII          84  CCGG                 C^CGG
MaeII         768  ACGT                 A^CGT
MamI          822  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          828  CATCCCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           84  CCGG                 C^CGG
NciI           84  CCGGG                CC^[GC]GG
NcoI          620  CCATGG               C^CATGG
PaeR7I        109  CTCGAG               C^TCGAG
PflMI         563  CCAGGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          314  TCATGA               T^CATGA
StuI          725  AGGCCT               AGG^CCT
Tsp45I        884  GTGAC                ^GT[GC]AC
Tsp509I       319  AATT                 ^AATT
Van91I        563  CCAGGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          260  CCATTGACAGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          109  CTCGAG               C^TCGAG
XmnI          346  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI           81  CCGC                 CCGC(-3/-1)
              538  CCGC                
AfaI          432  GTAC                 GT^AC
              699  GTAC                
AluI           94  AGCT                 AG^CT
              300  AGCT                
AlwI          686  GATCC                GGATC(4/5)
              796  GATCC               
AspI          246  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              274  GACAAGGTC           
BanII         737  GAGCCC               G[GA]GC[CT]^C
              863  GGGCCC              
BstYI         685  AGATCC               [GA]^GATC[CT]
              795  AGATCC              
DdeI          379  CTGAG                C^T[ACGT]AG
              551  CTAAG               
DsaI          620  CCATGG               C^C[GA][CT]GG
              859  CCACGG              
EarI          543  CTCTTC               CTCTTC(1/4)
              493  GAAGAG              
Ksp632I       543  CTCTTC               CTCTTC(1/4)
              493  GAAGAG              
MflI          685  AGATCC               [GA]^GATC[CT]
              795  AGATCC              
PflFI         246  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              274  GACAAGGTC           
PleI          129  GACTC                GAGTC(4/5)
              625  GACTC               
PpuMI         113  AGGACCC              [GA]G^G[AT]CC[CT]
              278  AGGTCCC             
RsaI          432  GTAC                 GT^AC
              699  GTAC                
SfaNI         385  GATGC                GCATC(5/9)
              508  GCATC               
SmlI           31  CTTGAG               C^T[CT][GA]AG
              109  CTCGAG              
TaqI          110  TCGA                 T^CGA
              438  TCGA                
TfiI          174  GATTC                G^A[AT]TC
              703  GATTC               
Tth111I       246  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              274  GACAAGGTC           
TthHB8I       110  TCGA                 T^CGA
              438  TCGA                
XhoII         685  AGATCC               [GA]^GATC[CT]
              795  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AvaII       BbvI        BfaI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstNI       Cac8I       CfoI        
Cfr13I      DpnII       DraII       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      HaeIII      HhaI        HinP1I      
HinfI       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       StyI        TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com