Restriction map for
pF1KA1191
(Circular) (Six-base) MAPSORT of: pF1KA1191.seq Check: 118 from: 1 to: 4028
pF1KA1191 4028 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 336 1066 336
Size: 730 3298
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 920 1853 2285 2638 3300 784
Size: 136 933 432 353 662 1512
Fragments arranged by size:
1512 933 662 432 353 136
AflIII A'CryG_T
Cuts at: 1380 3180 3461 1380
Size: 1800 281 1947
Fragments arranged by size:
1947 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 677 1333 2771 677
Size: 656 1438 1934
Fragments arranged by size:
1934 1438 656
ApaI G_GGCC'C
Cuts at: 975 975
Size: 4028
ApaLI G'TGCA_C
Cuts at: 2866 2866
Size: 4028
ApoI r'AATT_y
Cuts at: 426 1032 3381 3637 426
Size: 606 2349 256 817
Fragments arranged by size:
2349 817 606 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 217 1048 217
Size: 831 3197
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 1044 1458 1706 1741 1044
Size: 414 248 35 3331
Fragments arranged by size:
3331 414 248 35
BanII G_rGCy'C
Cuts at: 849 975 1042 2072 849
Size: 126 67 1030 2805
Fragments arranged by size:
2805 1030 126 67
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 208 242 208
Size: 34 3994
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2982 4028 1926
Size: 1056 1046 1926
Fragments arranged by size:
1926 1056 1046
BclI T'GATC_A
Cuts at: 434 1548 434
Size: 1114 2914
BglI GCCn_nnn'nGGC
Cuts at: 832 832
Size: 4028
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 882 975 1653 1746 2870 882
Size: 93 678 93 1124 2040
Fragments arranged by size:
2040 1124 678 93 93
BmrI ACTGGGnnnn_n'
Cuts at: 385 618 934 1240 1321 1656 3800 385
Size: 233 316 306 81 335 2144 613
Fragments arranged by size:
2144 613 335 316 306 233 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
Bpu10I CC'TnA_GC
Cuts at: 659 659
Size: 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 1204 2083 2797 3095 160
Size: 1044 879 714 298 1093
Fragments arranged by size:
1093 1044 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 526 3732 526
Size: 3206 822
BsaAI yAC'GTr
Cuts at: 877 1381 877
Size: 504 3524
BsaBI GATnn'nnATC
Cuts at: 6 934 1542 3579 6
Size: 928 608 2037 455
Fragments arranged by size:
2037 928 608 455
BsaHI Gr'CG_yC
Cuts at: 1459 1707 2409 3905 1459
Size: 248 702 1496 1582
Fragments arranged by size:
1582 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1217 1738 2416 2827 2974 3248 1217
Size: 521 678 411 147 274 1997
Fragments arranged by size:
1997 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 228 258 523 553 228
Size: 30 265 30 3703
Fragments arranged by size:
3703 265 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 230 233 544 857 230
Size: 3 311 313 3401
Fragments arranged by size:
3401 313 311 3
BseYI C'CCAG_C
Cuts at: 1453 2211 2876 3284 1453
Size: 758 665 408 2197
Fragments arranged by size:
2197 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1570 1616 2846 104
Size: 1466 46 1230 1286
Fragments arranged by size:
1466 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1007 1042 1820 2010 2870 1007
Size: 35 778 190 860 2165
Fragments arranged by size:
2165 860 778 190 35
BsmI GAATG_Cn'
Cuts at: 458 458
Size: 4028
Bsp1286I G_dGCh'C
Cuts at: 849 882 975 1007 1042 1653 1746 1820 2010
Size: 33 93 32 35 611 93 74 190
Cuts at: 2010 2072 2870 849
Size: 62 798 2007
Fragments arranged by size:
2007 798 611 190 93 93 74 62
35 33 32
BspHI T'CATG_A
Cuts at: 422 2460 422
Size: 2038 1990
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1078 1292 1594 1975 1078
Size: 214 302 381 3131
Fragments arranged by size:
3131 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2320 2374 3858 4023 50
Size: 2270 54 1484 165 55
Fragments arranged by size:
2270 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 1940
Size: 4028
BsrFI r'CCGG_y
Cuts at: 2026 2207 2026
Size: 181 3847
BssSI C'ACGA_G
Cuts at: 2299 3007 2299
Size: 708 3320
BstAPI GCAn_nnn'nTGC
Cuts at: 1405 1405
Size: 4028
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstXI CCAn_nnnn'nTGG
Cuts at: 894 894
Size: 4028
BstYI r'GATC_y
Cuts at: 1 793 903 1053 1520 1543 1878 2124 2430
Size: 792 110 150 467 23 335 246 306
Cuts at: 2430 2442 2528 3580 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 792 467 449 335 306 246 150
110 86 23 12
BtgI C'CryG_G
Cuts at: 107 728 967 107
Size: 621 239 3168
Fragments arranged by size:
3168 621 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 1271 1271
Size: 4028
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 1734 3078 1734
Size: 1344 2684
EaeI y'GGCC_r
Cuts at: 551 1613 1787 2178 2205 3550 551
Size: 1062 174 391 27 1345 1029
Fragments arranged by size:
1345 1062 1029 391 174 27
EagI C'GGCC_G
Cuts at: 1613 1613
Size: 4028
EarI CTCTTCn'nnn_
Cuts at: 596 657 2051 2261 596
Size: 61 1394 210 2363
Fragments arranged by size:
2363 1394 210 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 2978 3124 3838 2236
Size: 742 146 714 2426
Fragments arranged by size:
2426 742 714 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 920 1853 2285 2638 3300 784
Size: 136 933 432 353 662 1512
Fragments arranged by size:
1512 933 662 432 353 136
EcoNI CCTnn'n_nnAGG
Cuts at: 310 310
Size: 4028
EcoO109I rG'GnC_Cy
Cuts at: 222 387 516 1167 222
Size: 165 129 651 3083
Fragments arranged by size:
3083 651 165 129
EcoRI G'AATT_C
Cuts at: 426 1032 426
Size: 606 3422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 4028
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 1462 1710 2940 3711 1462
Size: 248 1230 771 1779
Fragments arranged by size:
1779 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 228 260 1870 1900 1902 1932 228
Size: 32 1610 30 2 30 2324
Fragments arranged by size:
2324 1610 32 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2787 2971 2787
Size: 184 3844
MscI TGG'CCA
Cuts at: 1789 1789
Size: 4028
MslI CAynn'nnrTG
Cuts at: 940 2017 2144 940
Size: 1077 127 2824
Fragments arranged by size:
2824 1077 127
MspA1I CmG'CkG
Cuts at: 1139 1453 1813 2597 2842 1139
Size: 314 360 784 245 2325
Fragments arranged by size:
2325 784 360 314 245
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NcoI C'CATG_G
Cuts at: 107 728 107
Size: 621 3407
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NspI r_CATG'y
Cuts at: 1081 1581 3184 1081
Size: 500 1603 1925
Fragments arranged by size:
1925 1603 500
PasI CC'CwG_GG
Cuts at: 671 671
Size: 4028
PciI A'CATG_T
Cuts at: 3180 3180
Size: 4028
PflMI CCAn_nnn'nTGG
Cuts at: 677 677
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2474 2506 2474
Size: 32 3996
PpuMI rG'GwC_Cy
Cuts at: 222 387 222
Size: 165 3863
PspOMI G'GGCC_C
Cuts at: 971 971
Size: 4028
PspXI vC'TCGA_Gb
Cuts at: 217 217
Size: 4028
PstI C_TGCA'G
Cuts at: 1075 1760 1075
Size: 685 3343
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 1453 1813 1453
Size: 360 3668
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 2051 2261 2051
Size: 210 3818
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 1071 1756 2724 2915 33
Size: 1038 685 968 191 1146
Fragments arranged by size:
1146 1038 968 685 191
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 1050 1050
Size: 4028
SmlI C'TyrA_G
Cuts at: 139 217 1183 2098 2812 3074 139
Size: 78 966 915 714 262 1093
Fragments arranged by size:
1093 966 915 714 262 78
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
StuI AGG'CCT
Cuts at: 835 835
Size: 4028
StyI C'CwwG_G
Cuts at: 107 268 311 728 1162 1516 107
Size: 161 43 417 434 354 2619
Fragments arranged by size:
2619 434 417 354 161 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 804 844 1026 1611 1744 2203 2412 804
Size: 40 182 585 133 459 209 2420
Fragments arranged by size:
2420 585 459 209 182 133 40
TatI w'GTAC_w
Cuts at: 3543 3543
Size: 4028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 3996
Tth111I GACn'n_nGTC
Cuts at: 357 385 1825 357
Size: 28 1440 2560
Fragments arranged by size:
2560 1440 28
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 375 1523 375
Size: 1148 2880
XhoI C'TCGA_G
Cuts at: 217 217
Size: 4028
XmaI C'CCGG_G
Cuts at: 1048 1048
Size: 4028
XmnI GAAnn'nnTTC
Cuts at: 458 458
Size: 4028
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI
BmtI BpmI BsgI BsiWI BsmBI BspEI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI