Restriction map for pF1KA1179
##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

AatII       AccIII      AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AspEI       
BcgI        BclI        BfrI        BsaBI       BseAI       
BsiEI       BsiWI       BsmBI       BspEI       BssHII      
Bst1107I    BstZ17I     CpoI        DraI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRV       
FbaI        FseI        HindIII     KspI        MamI        
MluI        MroI        NdeI        NotI        NruI        
PacI        PinAI       PmeI        PshAI       PvuI        
RsrII       SacII       SalI        ScaI        SgrAI       
SnaBI       SpeI        SphI        SspI        SwaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        520  GGTACC               G^GTACC
AccII        4628  CGCG                 CG^CG
AclI         1097  AACGTT               AA^CGTT
AcyI         5002  GGCGCC               G[GA]^CG[CT]C
ApaI         3674  GGGCCC               GGGCC^C
Asp718I       520  GGTACC               G^GTACC
AviII        3737  TGCGCA               TGC^GCA
AvrII        1086  CCTAGG               C^CTAGG
BamHI         803  GGATCC               G^GATCC
BbeI         5002  GGCGCC               GGCGC^C
BbrPI        3265  CACGTG               CAC^GTG
BbsI         4165  GAAGAC               GAAGAC(2/6)
BlnI         1086  CCTAGG               C^CTAGG
BpuAI        4165  GAAGAC               GAAGAC(2/6)
BsaHI        5002  GGCGCC               G[GA]^CG[CT]C
BspDI        4093  ATCGAT               AT^CGAT
BsrFI         360  GCCGGC               [GA]^CCGG[CT]
BssSI        2749  CTCGTG               CACGAG(-5/-1)
BstBI        3472  TTCGAA               TT^CGAA
BstEII         54  GGTGACC              G^GT[ACGT]ACC
BstPI          54  GGTGACC              G^GT[ACGT]ACC
BstUI        4628  CGCG                 CG^CG
Cfr10I        360  GCCGGC               [GA]^CCGG[CT]
ClaI         4093  ATCGAT               AT^CGAT
EcoO65I        54  GGTGACC              G^GT[ACGT]ACC
FspI         3737  TGCGCA               TGC^GCA
HaeII        5002  GGCGCC               [GA]GCGC^[CT]
Hin1I        5002  GGCGCC               G[GA]^CG[CT]C
HpaI          580  GTTAAC               GTT^AAC
KasI         5002  GGCGCC               G^GCGCC
KpnI          520  GGTACC               GGTAC^C
MvnI         4628  CGCG                 CG^CG
NaeI          360  GCCGGC               GCC^GGC
NarI         5002  GGCGCC               GG^CGCC
NgoMIV        360  GCCGGC               G^CCGGC
NspV         3472  TTCGAA               TT^CGAA
PaeR7I       3157  CTCGAG               C^TCGAG
PmaCI        3265  CACGTG               CAC^GTG
PmlI         3265  CACGTG               CAC^GTG
Psp1406I     1097  AACGTT               AA^CGTT
SapI          192  GAAGAGC              GCTCTTC(1/4)
SbfI         2772  CCTGCAGG             CCTGCA^GG
SfiI         3618  GGCCCGGGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         5002  GGCGCC               GGC^GCC
SfuI         3472  TTCGAA               TT^CGAA
Sse8387I     2772  CCTGCAGG             CCTGCA^GG
XbaI         4833  TCTAGA               T^CTAGA
XhoI         3157  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          274  GTCTAC               GT^[AC][GT]AC
             4717  GTAGAC              
AseI         1333  ATTAAT               AT^TAAT
             2620  ATTAAT              
AsnI         1333  ATTAAT               AT^TAAT
             2620  ATTAAT              
BalI         3230  TGGCCA               TGG^CCA
             5150  TGGCCA              
BbvCI        2791  GCTGAGG              CCTCAGC(-5/-2)
             3571  GCTGAGG             
BglI         3619  GCCCGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5005  GCCTACGAGGC         
BglII        2709  AGATCT               A^GATCT
             3075  AGATCT              
BsaAI         460  TACGTG               [CT]AC^GT[GA]
             3265  CACGTG              
BspHI        1304  TCATGA               T^CATGA
             2513  TCATGA              
BspLU11I     2837  ACATGT               A^CATGT
             3188  ACATGT              
DraIII        914  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2587  CACCTGGTG           
DrdI          109  GACCGAGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              301  GACAAGAAAGTC        
HgaI         3730  GACGC                GACGC(5/10)
             4695  GCGTC               
MfeI         2657  CAATTG               C^AATTG
             3366  CAATTG              
MluNI        3230  TGGCCA               TGG^CCA
             5150  TGGCCA              
MscI         3230  TGGCCA               TGG^CCA
             5150  TGGCCA              
MunI         2657  CAATTG               C^AATTG
             3366  CAATTG              
NcoI         2132  CCATGG               C^CATGG
             4958  CCATGG              
NheI         2298  GCTAGC               G^CTAGC
             2376  GCTAGC              
PshBI        1333  ATTAAT               AT^TAAT
             2620  ATTAAT              
RcaI         1304  TCATGA               T^CATGA
             2513  TCATGA              
SexAI        2588  ACCTGGT              A^CC[AT]GGT
             4547  ACCTGGT             
SmaI         2012  CCCGGG               CCC^GGG
             3620  CCCGGG              
XmaCI        2012  CCCGGG               C^CCGGG
             3620  CCCGGG              
XmaI         2012  CCCGGG               C^CCGGG
             3620  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  130 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       AocI        ApoI        Asp700I     
AspHI       AspI        AvaI        AvaII       BanI        
BanII       BbvI        BciVI       BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspMI       BsrBI       
BsrDI       BsrGI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRI       EcoRII      
EcoT14I     EcoT22I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PflFI       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII       XmnI        
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