Restriction map for pF1KA1168
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# NO CUT ENZYMES (   62 enzymes) #
##################################

AatII       Acc65I      AflII       AgeI        Aor51HI     
AscI        Asp718I     AviII       AvrII       BbeI        
BbrPI       BfrI        BlnI        BsaAI       BsaBI       
BsiWI       BsmBI       BspDI       BssHII      BssSI       
Bst1107I    BstZ17I     ClaI        CpoI        DrdI        
EagI        EclXI       Eco47III    Eco52I      FseI        
FspI        HindIII     KasI        KpnI        MamI        
MluI        NaeI        NarI        NgoMIV      NheI        
NotI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PvuI        RsrII       
SalI        ScaI        SfiI        SfoI        SgrAI       
SnaBI       SpeI        SphI        SspI        SwaI        
XbaI        XhoI        


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# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccIII       3206  TCCGGA               T^CCGGA
AclI         2246  AACGTT               AA^CGTT
AhdI         1829  GACCCATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        999  GTGCAC               G^TGCAC
ApaI         3402  GGGCCC               GGGCC^C
ApaLI         999  GTGCAC               G^TGCAC
AseI          868  ATTAAT               AT^TAAT
AsnI          868  ATTAAT               AT^TAAT
Asp700I        52  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1829  GACCCATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI        2289  GTATCC               GTATCC(6/5)
BlpI         3517  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3517  GCTGAGC              GC^T[ACGT]AGC
BsaI          958  GAGACC               GGTCTC(1/5)
BseAI        3206  TCCGGA               T^CCGGA
BsiEI        3687  CGACCG               CG[GA][CT]^CG
BspEI        3206  TCCGGA               T^CCGGA
BspHI         365  TCATGA               T^CATGA
BspLU11I      497  ACATGT               A^CATGT
BstBI        1538  TTCGAA               TT^CGAA
BstXI         632  CCATGTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        3517  GCTGAGC              GC^T[ACGT]AGC
DraI         2116  TTTAAA               TTT^AAA
Eam1105I     1829  GACCCATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1945  GAATTC               G^AATTC
EcoRV        1627  GATATC               GAT^ATC
HpaI         1959  GTTAAC               GTT^AAC
KspI         1289  CCGCGG               CCGC^GG
MfeI         2354  CAATTG               C^AATTG
MroI         3206  TCCGGA               T^CCGGA
MunI         2354  CAATTG               C^AATTG
NspV         1538  TTCGAA               TT^CGAA
PshBI         868  ATTAAT               AT^TAAT
Psp1406I     2246  AACGTT               AA^CGTT
RcaI          365  TCATGA               T^CATGA
SacII        1289  CCGCGG               CCGC^GG
SexAI        3245  ACCTGGT              A^CC[AT]GGT
SfuI         1538  TTCGAA               TT^CGAA
SmaI          269  CCCGGG               CCC^GGG
XcmI         2453  CCATTGTGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         269  CCCGGG               C^CCGGG
XmaI          269  CCCGGG               C^CCGGG
XmnI           52  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AccI         2179  GTCTAC               GT^[AC][GT]AC
             3175  GTCTAC              
AcyI         2275  GGCGTC               G[GA]^CG[CT]C
             3421  GGCGTC              
AocI         1809  CCTGAGG              CC^T[ACGT]AGG
             3060  CCTGAGG             
AspI          451  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              718  GACATTGTC           
AvaI          269  CCCGGG               C^[CT]CG[GA]G
             1686  CCCGAG              
BamHI        2740  GGATCC               G^GATCC
             2984  GGATCC              
BbvCI        3277  CCTCAGC              CCTCAGC(-5/-2)
             2149  GCTGAGG             
BcgI         1726  CGAAGAGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              389  GCAAGGCCATCG        
BclI         1367  TGATCA               T^GATCA
             2169  TGATCA              
BfaI         1174  CTAG                 C^TAG
             3170  CTAG                
BglI         1479  GCCCCTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1724  GCCGAAGAGGC         
BsaHI        2275  GGCGTC               G[GA]^CG[CT]C
             3421  GGCGTC              
BsaWI        3206  TCCGGA               [AT]^CCGG[AT]
             3689  ACCGGA              
BsgI          534  GTGCAG               GTGCAG(16/14)
             3628  GTGCAG              
BsmI          147  GAATGC               GAATGC(1/-1)
             3368  GCATTC              
BsoBI         269  CCCGGG               C^[CT]CG[GA]G
             1686  CCCGAG              
BsrFI        2486  ACCGGC               [GA]^CCGG[CT]
             3455  ACCGGC              
BstAPI        906  GCAGGTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2496  GCATCGGCTGC         
BstEII        354  GGTCACC              G^GT[ACGT]ACC
             3304  GGTGACC             
BstPI         354  GGTCACC              G^GT[ACGT]ACC
             3304  GGTGACC             
Bsu36I       1809  CCTGAGG              CC^T[ACGT]AGG
             3060  CCTGAGG             
Cfr10I       2486  ACCGGC               [GA]^CCGG[CT]
             3455  ACCGGC              
DraIII        660  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2560  CACAATGTG           
Eco81I       1809  CCTGAGG              CC^T[ACGT]AGG
             3060  CCTGAGG             
EcoO65I       354  GGTCACC              G^GT[ACGT]ACC
             3304  GGTGACC             
EcoT22I        81  ATGCAT               ATGCA^T
              149  ATGCAT              
FbaI         1367  TGATCA               T^GATCA
             2169  TGATCA              
Hin1I        2275  GGCGTC               G[GA]^CG[CT]C
             3421  GGCGTC              
HincII        724  GTCAAC               GT[CT]^[GA]AC
             1959  GTTAAC              
HindII        724  GTCAAC               GT[CT]^[GA]AC
             1959  GTTAAC              
MaeI         1174  CTAG                 C^TAG
             3170  CTAG                
NcoI         1964  CCATGG               C^CATGG
             2219  CCATGG              
NdeI          781  CATATG               CA^TATG
             2515  CATATG              
NruI         1158  TCGCGA               TCG^CGA
             3297  TCGCGA              
NsiI           81  ATGCAT               ATGCA^T
              149  ATGCAT              
PflFI         451  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              718  GACATTGTC           
SbfI         1377  CCTGCAGG             CCTGCA^GG
             3381  CCTGCAGG            
SmlI          790  CTCAAG               C^T[CT][GA]AG
             1593  CTTGAG              
Sse8387I     1377  CCTGCAGG             CCTGCA^GG
             3381  CCTGCAGG            
Tth111I       451  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              718  GACATTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BcnI        BglII       BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    Bsp1407I    
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspBI       StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       
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