Restriction map for pF1KA1168
 (Linear) (Six-base) MAPSORT of: KIAA1168.seq  Check: 9458  from: 1  to: 3834



KIAA1168.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     897    1371    3626    3834 
   Size:        897     474    2255     208

  Fragments arranged by size: 

                2255     897     474     208
 
AccI GT'mk_AC

Cuts at:      0    2180    3176    3834 
   Size:       2180     996     658

  Fragments arranged by size: 

                2180     996     658
 
AclI AA'CG_TT

Cuts at:      0    2247    3834 
   Size:       2247    1587
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2341    3031    3694    3834 
   Size:       2341     690     663     140

  Fragments arranged by size: 

                2341     690     663     140
 
AflIII A'CryG_T

Cuts at:      0     497    2331    3047    3834 
   Size:        497    1834     716     787

  Fragments arranged by size: 

                1834     787     716     497
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1834    3834 
   Size:       1834    2000
 
AlwNI CAG_nnn'CTG

Cuts at:      0     582    2130    3060    3834 
   Size:        582    1548     930     774

  Fragments arranged by size: 

                1548     930     774     582
 
ApaI G_GGCC'C

Cuts at:      0    3406    3834 
   Size:       3406     428
 
ApaLI G'TGCA_C

Cuts at:      0     999    3834 
   Size:        999    2835
 
ApoI r'AATT_y

Cuts at:      0     889    1339    1945    2790    3834 
   Size:        889     450     606     845    1044

  Fragments arranged by size: 

                1044     889     845     606     450
 
AseI AT'TA_AT

Cuts at:      0     869    3834 
   Size:        869    2965
 
AvaI C'yCGr_G

Cuts at:      0     269    1686    3834 
   Size:        269    1417    2148

  Fragments arranged by size: 

                2148    1417     269
 
BamHI G'GATC_C

Cuts at:      0    2740    2984    3834 
   Size:       2740     244     850

  Fragments arranged by size: 

                2740     850     244
 
BanI G'GyrC_C

Cuts at:      0     912    1285    1635    3834 
   Size:        912     373     350    2199

  Fragments arranged by size: 

                2199     912     373     350
 
BanII G_rGCy'C

Cuts at:      0    1088    1123    1166    1481    1577    3038    3406    3834 
   Size:       1088      35      43     315      96    1461     368     428

  Fragments arranged by size: 

                1461    1088     428     368     315      96      43      35
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     334    1401    1810    2592    3834 
   Size:        334    1067     409     782    1242

  Fragments arranged by size: 

                1242    1067     782     409     334
 
BbvCI CC'TCA_GC

Cuts at:      0    2150    3278    3834 
   Size:       2150    1128     556

  Fragments arranged by size: 

                2150    1128     556
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     378     412    1715    1749    3834 
   Size:        378      34    1303      34    2085

  Fragments arranged by size: 

                2085    1303     378      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2300    3834 
   Size:       2300    1534
 
BclI T'GATC_A

Cuts at:      0    1367    2169    3834 
   Size:       1367     802    1665

  Fragments arranged by size: 

                1665    1367     802
 
BfrBI ATG'CAT

Cuts at:      0      83     151    3834 
   Size:         83      68    3683

  Fragments arranged by size: 

                3683      83      68
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1485    1730    3834 
   Size:       1485     245    2104

  Fragments arranged by size: 

                2104    1485     245
 
BglII A'GATC_T

Cuts at:      0     317    2198    3707    3834 
   Size:        317    1881    1509     127

  Fragments arranged by size: 

                1881    1509     317     127
 
BlpI GC'TnA_GC

Cuts at:      0    3518    3834 
   Size:       3518     316
 
Bme1580I G_kGCm'C

Cuts at:      0     917    1003    1640    3406    3671    3834 
   Size:        917      86     637    1766     265     163

  Fragments arranged by size: 

                1766     917     637     265     163      86
 
BmgBI CAC'GTC

Cuts at:      0      12     998    1218    2334    3048    3432    3834 
   Size:         12     986     220    1116     714     384     402

  Fragments arranged by size: 

                1116     986     714     402     384     220      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     597     657     764    1017    1566    3555    3834 
   Size:        597      60     107     253     549    1989     279

  Fragments arranged by size: 

                1989     597     549     279     253     107      60
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1248    2991    3834 
   Size:       1248    1743     843

  Fragments arranged by size: 

                1743    1248     843
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     238     364    1975    2485    2497    2893    3010 
   Size:        183      55     126    1611     510      12     396     117

Cuts at:   3010    3220    3834 
   Size:        210     614

  Fragments arranged by size: 

                1611     614     510     396     210     183     126     117
                  55      12
 
Bpu10I CC'TnA_GC

Cuts at:      0    1122    2150    3278    3834 
   Size:       1122    1028    1128     556

  Fragments arranged by size: 

                1128    1122    1028     556
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     775    1614    3834 
   Size:        775     839    2220

  Fragments arranged by size: 

                2220     839     775
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     952    3834 
   Size:        952    2882
 
BsaHI Gr'CG_yC

Cuts at:      0    2276    3422    3834 
   Size:       2276    1146     412

  Fragments arranged by size: 

                2276    1146     412
 
BsaWI w'CCGG_w

Cuts at:      0    3206    3689    3834 
   Size:       3206     483     145

  Fragments arranged by size: 

                3206     483     145
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     770     800    2434    2452    2464    2482    3313    3343 
   Size:        770      30    1634      18      12      18     831      30

Cuts at:   3343    3834 
   Size:        491

  Fragments arranged by size: 

                1634     831     770     491      30      30      18      18
                  12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     718     778    1165    1827    3085    3136    3834 
   Size:        718      60     387     662    1258      51     698

  Fragments arranged by size: 

                1258     718     698     662     387      60      51
 
BseYI C'CCAG_C

Cuts at:      0    1928    2378    3267    3573    3834 
   Size:       1928     450     889     306     261

  Fragments arranged by size: 

                1928     889     450     306     261
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     555    3649    3834 
   Size:        555    3094     185

  Fragments arranged by size: 

                3094     555     185
 
BsiEI CG_ry'CG

Cuts at:      0    3690    3834 
   Size:       3690     144
 
BsiHKAI G_wGCw'C

Cuts at:      0     969    1003    1088    1166    1388    3038    3834 
   Size:        969      34      85      78     222    1650     796

  Fragments arranged by size: 

                1650     969     796     222      85      78      34
 
BsmI GAATG_Cn'

Cuts at:      0     153    3368    3834 
   Size:        153    3215     466

  Fragments arranged by size: 

                3215     466     153
 
Bsp1286I G_dGCh'C

Cuts at:      0     917     969    1003    1088    1123    1166    1388    1481 
   Size:        917      52      34      85      35      43     222      93

Cuts at:   1481    1577    1640    3038    3406    3671    3834 
   Size:         96      63    1398     368     265     163

  Fragments arranged by size: 

                1398     917     368     265     222     163      96      93
                  85      63      52      43      35      34
 
BspEI T'CCGG_A

Cuts at:      0    3206    3834 
   Size:       3206     628
 
BspHI T'CATG_A

Cuts at:      0     365    3834 
   Size:        365    3469
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      53     897    1371    2718    3389    3626    3834 
   Size:         53     844     474    1347     671     237     208

  Fragments arranged by size: 

                1347     844     671     474     237     208      53
 
BsrBI CCG'CTC

Cuts at:      0     402    3240    3264    3324    3834 
   Size:        402    2838      24      60     510

  Fragments arranged by size: 

                2838     510     402      60      24
 
BsrDI GCAATG_nn'

Cuts at:      0     164    1444    1778    2756    3081    3290    3834 
   Size:        164    1280     334     978     325     209     544

  Fragments arranged by size: 

                1280     978     544     334     325     209     164
 
BsrFI r'CCGG_y

Cuts at:      0    2486    3455    3834 
   Size:       2486     969     379

  Fragments arranged by size: 

                2486     969     379
 
BsrGI T'GTAC_A

Cuts at:      0     248    1748    2081    3834 
   Size:        248    1500     333    1753

  Fragments arranged by size: 

                1753    1500     333     248
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     912    2502    3834 
   Size:        912    1590    1332

  Fragments arranged by size: 

                1590    1332     912
 
BstBI TT'CG_AA

Cuts at:      0    1539    3834 
   Size:       1539    2295
 
BstEII G'GTnAC_C

Cuts at:      0     354    3304    3834 
   Size:        354    2950     530

  Fragments arranged by size: 

                2950     530     354
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     639    3834 
   Size:        639    3195
 
BstYI r'GATC_y

Cuts at:      0     317     597    1052    2076    2198    2740    2984    3707 
   Size:        317     280     455    1024     122     542     244     723

Cuts at:   3707    3834 
   Size:        127

  Fragments arranged by size: 

                1024     723     542     455     317     280     244     127
                 122
 
Bsu36I CC'TnA_GG

Cuts at:      0    1810    3061    3834 
   Size:       1810    1251     773

  Fragments arranged by size: 

                1810    1251     773
 
BtgI C'CryG_G

Cuts at:      0    1289    1964    2219    3755    3834 
   Size:       1289     675     255    1536      79

  Fragments arranged by size: 

                1536    1289     675     255      79
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2832    3548    3834 
   Size:       2832     716     286

  Fragments arranged by size: 

                2832     716     286
 
BtsI GCAGTG_nn'

Cuts at:      0    1918    2235    2314    3529    3834 
   Size:       1918     317      79    1215     305

  Fragments arranged by size: 

                1918    1215     317     305      79
 
DraI TTT'AAA

Cuts at:      0    2118    3834 
   Size:       2118    1716
 
DraIII CAC_nnn'GTG

Cuts at:      0     665    2565    3834 
   Size:        665    1900    1269

  Fragments arranged by size: 

                1900    1269     665
 
EaeI y'GGCC_r

Cuts at:      0     641     944    2580    3683    3821    3834 
   Size:        641     303    1636    1103     138      13

  Fragments arranged by size: 

                1636    1103     641     303     138      13
 
EarI CTCTTCn'nnn_

Cuts at:      0      47     856     971    1171    1722    2878    3091    3834 
   Size:         47     809     115     200     551    1156     213     743

  Fragments arranged by size: 

                1156     809     743     551     213     200     115      47
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2559    3834 
   Size:       2559    1275
 
EcoICRI GAG'CTC

Cuts at:      0    1086    1164    3036    3834 
   Size:       1086      78    1872     798

  Fragments arranged by size: 

                1872    1086     798      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     238     364    1975    2341    2485    2497    2893 
   Size:        183      55     126    1611     366     144      12     396

Cuts at:   2893    3010    3031    3220    3694    3834 
   Size:        117      21     189     474     140

  Fragments arranged by size: 

                1611     474     396     366     189     183     144     140
                 126     117      55      21      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     564    1486    3316    3834 
   Size:        564     922    1830     518

  Fragments arranged by size: 

                1830     922     564     518
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1608    2442    3272    3387    3402    3834 
   Size:       1608     834     830     115      15     432

  Fragments arranged by size: 

                1608     834     830     432     115      15
 
EcoRI G'AATT_C

Cuts at:      0    1945    3834 
   Size:       1945    1889
 
EcoRV GAT'ATC

Cuts at:      0    1629    3834 
   Size:       1629    2205
 
HaeII r_GCGC'y

Cuts at:      0    1214    2096    3198    3470    3834 
   Size:       1214     882    1102     272     364

  Fragments arranged by size: 

                1214    1102     882     364     272
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     302     334     442     474     582     589 
   Size:         16      32     254      32     108      32     108       7

Cuts at:    589     614     621     855     887     951     970     983    1002 
   Size:         25       7     234      32      64      19      13      19

Cuts at:   1002    1613    1645    1820    1852    2434    2466    3164    3196 
   Size:        611      32     175      32     582      32     698      32

Cuts at:   3196    3201    3233    3834 
   Size:          5      32     601

  Fragments arranged by size: 

                 698     611     601     582     254     234     175     108
                 108      64      32      32      32      32      32      32
                  32      32      32      25      19      19      16      13
                   7       7       5
 
HincII GTy'rAC

Cuts at:      0     726    1961    3834 
   Size:        726    1235    1873

  Fragments arranged by size: 

                1873    1235     726
 
HpaI GTT'AAC

Cuts at:      0    1961    3834 
   Size:       1961    1873
 
MfeI C'AATT_G

Cuts at:      0    2354    3834 
   Size:       2354    1480
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      59    2219    3834 
   Size:         59    2160    1615

  Fragments arranged by size: 

                2160    1615      59
 
MscI TGG'CCA

Cuts at:      0     643     946    3823    3834 
   Size:        643     303    2877      11

  Fragments arranged by size: 

                2877     643     303      11
 
MslI CAynn'nnrTG

Cuts at:      0     233     496     733    1225    1993    2851    2969    3434 
   Size:        233     263     237     492     768     858     118     465

Cuts at:   3434    3493    3834 
   Size:         59     341

  Fragments arranged by size: 

                 858     768     492     465     341     263     237     233
                 118      59
 
MspA1I CmG'CkG

Cuts at:      0     263     903    1291    2340    3009    3517    3834 
   Size:        263     640     388    1049     669     508     317

  Fragments arranged by size: 

                1049     669     640     508     388     317     263
 
NcoI C'CATG_G

Cuts at:      0    1964    2219    3834 
   Size:       1964     255    1615

  Fragments arranged by size: 

                1964    1615     255
 
NdeI CA'TA_TG

Cuts at:      0     782    2516    3834 
   Size:        782    1734    1318

  Fragments arranged by size: 

                1734    1318     782
 
NruI TCG'CGA

Cuts at:      0    1160    3299    3834 
   Size:       1160    2139     535

  Fragments arranged by size: 

                2139    1160     535
 
NsiI A_TGCA'T

Cuts at:      0      85     153    3834 
   Size:         85      68    3681

  Fragments arranged by size: 

                3681      85      68
 
NspI r_CATG'y

Cuts at:      0     501     933    2599    3787    3834 
   Size:        501     432    1666    1188      47

  Fragments arranged by size: 

                1666    1188     501     432      47
 
PasI CC'CwG_GG

Cuts at:      0    2980    3834 
   Size:       2980     854
 
PciI A'CATG_T

Cuts at:      0     497    3834 
   Size:        497    3337
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1221    2006    2028    2343    3819    3834 
   Size:       1221     785      22     315    1476      15

  Fragments arranged by size: 

                1476    1221     785     315      22      15
 
PfoI T'CCnGG_A

Cuts at:      0     509     611    1055    1952    2027    2987    3834 
   Size:        509     102     444     897      75     960     847

  Fragments arranged by size: 

                 960     897     847     509     444     102      75
 
PpuMI rG'GwC_Cy

Cuts at:      0    1608    2442    3272    3387    3834 
   Size:       1608     834     830     115     447

  Fragments arranged by size: 

                1608     834     830     447     115
 
PspOMI G'GGCC_C

Cuts at:      0    3402    3834 
   Size:       3402     432
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     742     774    1420    1452    3834 
   Size:        742      32     646      32    2382

  Fragments arranged by size: 

                2382     742     646      32      32
 
PstI C_TGCA'G

Cuts at:      0     412     908    1193    1382    1910    2827    3386    3834 
   Size:        412     496     285     189     528     917     559     448

  Fragments arranged by size: 

                 917     559     528     496     448     412     285     189
            
 
PvuII CAG'CTG

Cuts at:      0     263     903    2340    3009    3517    3834 
   Size:        263     640    1437     669     508     317

  Fragments arranged by size: 

                1437     669     640     508     317     263
 
SacI G_AGCT'C

Cuts at:      0    1088    1166    3038    3834 
   Size:       1088      78    1872     796

  Fragments arranged by size: 

                1872    1088     796      78
 
SacII CC_GC'GG

Cuts at:      0    1292    3834 
   Size:       1292    2542
 
SanDI GG'GwC_CC

Cuts at:      0    3272    3834 
   Size:       3272     562
 
SapI GCTCTTCn'nnn_

Cuts at:      0      47    1171    2878    3834 
   Size:         47    1124    1707     956

  Fragments arranged by size: 

                1707    1124     956      47
 
SbfI CC_TGCA'GG

Cuts at:      0    1382    3386    3834 
   Size:       1382    2004     448

  Fragments arranged by size: 

                2004    1382     448
 
SexAI A'CCwGG_T

Cuts at:      0    3245    3834 
   Size:       3245     589
 
SfcI C'TryA_G

Cuts at:      0     188     408     904    1095    1189    1378    1528    1906 
   Size:        188     220     496     191      94     189     150     378

Cuts at:   1906    2763    2775    2784    2823    3382    3834 
   Size:        857      12       9      39     559     452

  Fragments arranged by size: 

                 857     559     496     452     378     220     191     189
                 188     150      94      39      12       9
 
SmaI CCC'GGG

Cuts at:      0     271    3834 
   Size:        271    3563
 
SmlI C'TyrA_G

Cuts at:      0     790    1593    3834 
   Size:        790     803    2241

  Fragments arranged by size: 

                2241     803     790
 
StuI AGG'CCT

Cuts at:      0     466    1376    1672    3834 
   Size:        466     910     296    2162

  Fragments arranged by size: 

                2162     910     466     296
 
StyI C'CwwG_G

Cuts at:      0     480    1964    2219    2408    2895    3317    3818    3834 
   Size:        480    1484     255     189     487     422     501      16

  Fragments arranged by size: 

                1484     501     487     480     422     255     189      16
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     676    1018    2701    3819    3834 
   Size:        676     342    1683    1118      15

  Fragments arranged by size: 

                1683    1118     676     342      15
 
TatI w'GTAC_w

Cuts at:      0     248    1025    1229    1748    2081    3482    3834 
   Size:        248     777     204     519     333    1401     352

  Fragments arranged by size: 

                1401     777     519     352     333     248     204
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1849    1881    2452    2484    2551    2583    3313    3345 
   Size:       1849      32     571      32      67      32     730      32

Cuts at:   3345    3834 
   Size:        489

  Fragments arranged by size: 

                1849     730     571     489      67      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0     454     721    3834 
   Size:        454     267    3113

  Fragments arranged by size: 

                3113     454     267
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2460    3834 
   Size:       2460    1374
 
XmaI C'CCGG_G

Cuts at:      0     269    3834 
   Size:        269    3565
 
XmnI GAAnn'nnTTC

Cuts at:      0      56    3834 
   Size:         56    3778
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI   AflIII     AhdI    AlwNI     ApaI
    ApaLI     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII
     HpaI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NruI     NsiI     NspI     PasI     PciI    PflMI     PfoI    PpuMI
   PspOMI     PsrI     PstI    PvuII     SacI    SacII    SanDI     SapI
     SbfI    SexAI     SfcI     SmaI     SmlI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII     AgeI     AleI     AlfI     AloI
     AscI    AsiSI    AvrII     BaeI     BbeI     BmtI    BsaAI    BsaBI
    BsiWI    BsmBI   BssHII    BssSI  BstZ17I     ClaI    CspCI     DrdI
     EagI     FalI     FseI     FspI    FspAI  HindIII     KasI     KpnI
     MluI     NaeI     NarI   NgoMIV     NheI     NotI     PacI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PvuI    RsrII     SalI
     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XhoI     ZraI
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