Restriction map for pF1KA1160
 (Linear) (Six-base) MAPSORT of: KIAA1160.seq  Check: 1794  from: 1  to: 993



KIAA1160.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AflIII A'CryG_T

Cuts at:      0     439     993 
   Size:        439     554
 
ApoI r'AATT_y

Cuts at:      0     199     206     993 
   Size:        199       7     787

  Fragments arranged by size: 

                 787     199       7
 
BanI G'GyrC_C

Cuts at:      0     971     993 
   Size:        971      22
 
BanII G_rGCy'C

Cuts at:      0     452     960     993 
   Size:        452     508      33

  Fragments arranged by size: 

                 508     452      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      90     993 
   Size:         90     903
 
BglI GCCn_nnn'nGGC

Cuts at:      0      28     924     993 
   Size:         28     896      69

  Fragments arranged by size: 

                 896      69      28
 
BglII A'GATC_T

Cuts at:      0     155     856     993 
   Size:        155     701     137

  Fragments arranged by size: 

                 701     155     137
 
BlpI GC'TnA_GC

Cuts at:      0      53     446     993 
   Size:         53     393     547

  Fragments arranged by size: 

                 547     393      53
 
BmgBI CAC'GTC

Cuts at:      0     726     993 
   Size:        726     267
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     267     993 
   Size:        267     726
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     599     993 
   Size:        599     394
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     775     993 
   Size:        775     218
 
Bpu10I CC'TnA_GC

Cuts at:      0      33     241     993 
   Size:         33     208     752

  Fragments arranged by size: 

                 752     208      33
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     802     993 
   Size:        802     191
 
BsaAI yAC'GTr

Cuts at:      0     442     993 
   Size:        442     551
 
BsaBI GATnn'nnATC

Cuts at:      0     637     993 
   Size:        637     356
 
BsaWI w'CCGG_w

Cuts at:      0     266     993 
   Size:        266     727
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     416     611     625     628     637     670     673     769 
   Size:        416     195      14       3       9      33       3      96

Cuts at:    769     778     968     993 
   Size:          9     190      25

  Fragments arranged by size: 

                 416     195     190      96      33      25      14       9
                   9       3       3
 
BseYI C'CCAG_C

Cuts at:      0     279     993 
   Size:        279     714
 
BsiEI CG_ry'CG

Cuts at:      0     871     993 
   Size:        871     122
 
BsiHKAI G_wGCw'C

Cuts at:      0     452     960     993 
   Size:        452     508      33

  Fragments arranged by size: 

                 508     452      33
 
BsmI GAATG_Cn'

Cuts at:      0     189     993 
   Size:        189     804
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     129     993 
   Size:        129     864
 
Bsp1286I G_dGCh'C

Cuts at:      0     452     960     993 
   Size:        452     508      33

  Fragments arranged by size: 

                 508     452      33
 
BspEI T'CCGG_A

Cuts at:      0     266     993 
   Size:        266     727
 
BspHI T'CATG_A

Cuts at:      0     324     452     993 
   Size:        324     128     541

  Fragments arranged by size: 

                 541     324     128
 
BsrBI CCG'CTC

Cuts at:      0     881     993 
   Size:        881     112
 
BsrDI GCAATG_nn'

Cuts at:      0     716     993 
   Size:        716     277
 
BsrFI r'CCGG_y

Cuts at:      0     870     993 
   Size:        870     123
 
BstBI TT'CG_AA

Cuts at:      0     204     993 
   Size:        204     789
 
BstEII G'GTnAC_C

Cuts at:      0     481     993 
   Size:        481     512
 
BstYI r'GATC_y

Cuts at:      0     155     856     993 
   Size:        155     701     137

  Fragments arranged by size: 

                 701     155     137
 
BtsI GCAGTG_nn'

Cuts at:      0     920     993 
   Size:        920      73
 
ClaI AT'CG_AT

Cuts at:      0     464     993 
   Size:        464     529
 
EaeI y'GGCC_r

Cuts at:      0     868     872     993 
   Size:        868       4     121

  Fragments arranged by size: 

                 868     121       4
 
EagI C'GGCC_G

Cuts at:      0     868     993 
   Size:        868     125
 
EarI CTCTTCn'nnn_

Cuts at:      0      56     425     602     614     623     993 
   Size:         56     369     177      12       9     370

  Fragments arranged by size: 

                 370     369     177      56      12       9
 
EcoICRI GAG'CTC

Cuts at:      0     450     958     993 
   Size:        450     508      35

  Fragments arranged by size: 

                 508     450      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     775     993 
   Size:        775     218
 
EcoO109I rG'GnC_Cy

Cuts at:      0     286     993 
   Size:        286     707
 
EcoRI G'AATT_C

Cuts at:      0     206     993 
   Size:        206     787
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     436     993 
   Size:        436     557
 
FseI GG_CCGG'CC

Cuts at:      0     874     993 
   Size:        874     119
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     128     160     646     678     993 
   Size:        128      32     486      32     315

  Fragments arranged by size: 

                 486     315     128      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     644     993 
   Size:        644     349
 
MspA1I CmG'CkG

Cuts at:      0      57     993 
   Size:         57     936
 
NaeI GCC'GGC

Cuts at:      0     872     993 
   Size:        872     121
 
NgoMIV G'CCGG_C

Cuts at:      0     870     993 
   Size:        870     123
 
PmlI CAC'GTG

Cuts at:      0     442     993 
   Size:        442     551
 
PpuMI rG'GwC_Cy

Cuts at:      0     286     993 
   Size:        286     707
 
PstI C_TGCA'G

Cuts at:      0     818     856     993 
   Size:        818      38     137

  Fragments arranged by size: 

                 818     137      38
 
PvuII CAG'CTG

Cuts at:      0      57     993 
   Size:         57     936
 
SacI G_AGCT'C

Cuts at:      0     452     960     993 
   Size:        452     508      33

  Fragments arranged by size: 

                 508     452      33
 
SbfI CC_TGCA'GG

Cuts at:      0     818     993 
   Size:        818     175
 
ScaI AGT'ACT

Cuts at:      0     364     475     993 
   Size:        364     111     518

  Fragments arranged by size: 

                 518     364     111
 
SfcI C'TryA_G

Cuts at:      0     814     852     993 
   Size:        814      38     141

  Fragments arranged by size: 

                 814     141      38
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      28     993 
   Size:         28     965
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     912     993 
   Size:        912      81
 
TatI w'GTAC_w

Cuts at:      0     106     362     473     993 
   Size:        106     256     111     520

  Fragments arranged by size: 

                 520     256     111     106
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     843     993 
   Size:        843     150
 
XmnI GAAnn'nnTTC

Cuts at:      0     419     993 
   Size:        419     574
 

 Enzymes that do cut:

   AflIII     ApoI     BanI    BanII     BbsI     BglI    BglII     BlpI
    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BstBI   BstEII    BstYI
     BtsI     ClaI     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FseI    Hin4I     MmeI   MspA1I     NaeI   NgoMIV
     PmlI    PpuMI     PstI    PvuII     SacI     SbfI     ScaI     SfcI
     SfiI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI Bme1580I     BmtI    BpuEI
    BsaHI    BsaXI     BsgI    BsiWI    BspMI    BsrGI   BssHII    BssSI
   BstAPI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI     DraI
   DraIII     DrdI     EciI    EcoNI    EcoRV     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI    SexAI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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