Restriction map for pF1KA1154
 (Circular) (Six-base) MAPSORT of: pF1KA1154.seq  Check: 6719  from: 1  to: 4541



pF1KA1154  4541 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AccI GT'mk_AC

Cuts at:   1148    1442    1579    1148 
   Size:        294     137    4110

  Fragments arranged by size: 

                4110     294     137
 
Acc65I G'GTAC_C

Cuts at:   1557    1557 
   Size:       4541
 
AclI AA'CG_TT

Cuts at:   4381    4381 
   Size:       4541
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    271     496     949    2366    2798    3545    3813     271 
   Size:        225     453    1417     432     747     268     999

  Fragments arranged by size: 

                1417     999     747     453     432     268     225
 
AflIII A'CryG_T

Cuts at:    485    1893    2997    3974     485 
   Size:       1408    1104     977    1052

  Fragments arranged by size: 

                1408    1104    1052     977
 
AgeI A'CCGG_T

Cuts at:   2929    2929 
   Size:       4541
 
AlwNI CAG_nnn'CTG

Cuts at:    927    1846    3413     927 
   Size:        919    1567    2055

  Fragments arranged by size: 

                2055    1567     919
 
ApaLI G'TGCA_C

Cuts at:    522    3311     522 
   Size:       2789    1752
 
ApoI r'AATT_y

Cuts at:    957    1002    1545    3894    4150     957 
   Size:         45     543    2349     256    1348

  Fragments arranged by size: 

                2349    1348     543     256      45
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4541
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4541
 
AvaI C'yCGr_G

Cuts at:   1561    1561 
   Size:       4541
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1094    1127    1094 
   Size:         33    4508
 
BamHI G'GATC_C

Cuts at:    844     898    1566     844 
   Size:         54     668    3819

  Fragments arranged by size: 

                3819     668      54
 
BanI G'GyrC_C

Cuts at:    200     276    1327    1557    1971    2219    2254     200 
   Size:         76    1051     230     414     248      35    2487

  Fragments arranged by size: 

                2487    1051     414     248     230      76      35
 
BanII G_rGCy'C

Cuts at:   1555    2585    1555 
   Size:       1030    3511
 
BbeI G_GCGC'C

Cuts at:    204    1975    2223     204 
   Size:       1771     248    2522

  Fragments arranged by size: 

                2522    1771     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    237     956    1205     237 
   Size:        719     249    3573

  Fragments arranged by size: 

                3573     719     249
 
BbvCI CC'TCA_GC

Cuts at:    920     920 
   Size:       4541
 
BciVI GTATCCnnnnn_n'

Cuts at:   2439    3200    4541    2439 
   Size:        761    1341    2439

  Fragments arranged by size: 

                2439    1341     761
 
BclI T'GATC_A

Cuts at:   2061    2061 
   Size:       4541
 
BglII A'GATC_T

Cuts at:   2056    4093    2056 
   Size:       2037    2504
 
BlpI GC'TnA_GC

Cuts at:   1653    1653 
   Size:       4541
 
Bme1580I G_kGCm'C

Cuts at:    526    2166    2259    3315     526 
   Size:       1640      93    1056    1752

  Fragments arranged by size: 

                1752    1640    1056      93
 
BmgBI CAC'GTC

Cuts at:    954     954 
   Size:       4541
 
BmrI ACTGGGnnnn_n'

Cuts at:   1753    1834    2169    4313    1753 
   Size:         81     335    2144    1981

  Fragments arranged by size: 

                2144    1981     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2908    2908 
   Size:       4541
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    392    1070     392 
   Size:        678    3863
 
Bpu10I CC'TnA_GC

Cuts at:    920    1262     920 
   Size:        342    4199
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1391    1717    2596    3088    3386    1391 
   Size:        326     879     492     298    2546

  Fragments arranged by size: 

                2546     879     492     326     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    542    4245     542 
   Size:       3703     838
 
BsaAI yAC'GTr

Cuts at:    366    1894     366 
   Size:       1528    3013
 
BsaBI GATnn'nnATC

Cuts at:      6    2055    4092       6 
   Size:       2049    2037     455

  Fragments arranged by size: 

                2049    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    201    1972    2220    2922    4418     201 
   Size:       1771     248     702    1496     324

  Fragments arranged by size: 

                1771    1496     702     324     248
 
BsaWI w'CCGG_w

Cuts at:    329    1730    2251    2929    3203    3350    3761     329 
   Size:       1401     521     678     274     147     411    1109

  Fragments arranged by size: 

                1401    1109     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    660     908    1288    1351    1363     660 
   Size:        248     380      63      12    3838

  Fragments arranged by size: 

                3838     380     248      63      12
 
BseYI C'CCAG_C

Cuts at:    561    1966    2724    3301    3797     561 
   Size:       1405     758     577     496    1305

  Fragments arranged by size: 

                1405    1305     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    2083    2129    3337     104 
   Size:       1979      46    1208    1308

  Fragments arranged by size: 

                1979    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    526    1555    2333    2523    3315     526 
   Size:       1029     778     190     792    1752

  Fragments arranged by size: 

                1752    1029     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    526    1555    2166    2259    2333    2523    2585    3315     526 
   Size:       1029     611      93      74     190      62     730    1752

  Fragments arranged by size: 

                1752    1029     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:    329    3761     329 
   Size:       3432    1109
 
BspHI T'CATG_A

Cuts at:   3717    3717 
   Size:       4541
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1350    1591    1805    2107    2488    1350 
   Size:        241     214     302     381    3403

  Fragments arranged by size: 

                3403     381     302     241     214
 
BsrBI CCG'CTC

Cuts at:     50     113     353     650    2833    2887    4371    4536      50 
   Size:         63     240     297    2183      54    1484     165      55

  Fragments arranged by size: 

                2183    1484     297     240     165      63      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   2453    2453 
   Size:       4541
 
BsrFI r'CCGG_y

Cuts at:    262    2539    2720    2929     262 
   Size:       2277     181     209    1874

  Fragments arranged by size: 

                2277    1874     209     181
 
BssSI C'ACGA_G

Cuts at:   1313    2812    3170    1313 
   Size:       1499     358    2684

  Fragments arranged by size: 

                2684    1499     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1309    1918    1309 
   Size:        609    3932
 
BstBI TT'CG_AA

Cuts at:    239    2902     239 
   Size:       2663    1878
 
BstEII G'GTnAC_C

Cuts at:    258    1219     258 
   Size:        961    3580
 
BstYI r'GATC_y

Cuts at:      1     344     844     898    1566    2033    2056    2391    2637 
   Size:        343     500      54     668     467      23     335     246

Cuts at:   2637    3649    3735    3747    4093       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     668     500     467     449     346     343     335
                 246      86      54      23      12
 
BtgI C'CryG_G

Cuts at:    310    1529     310 
   Size:       1219    3322
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2491    2672    2835    2491 
   Size:        181     163    4197

  Fragments arranged by size: 

                4197     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1425    1784    1425 
   Size:        359    4182
 
DraI TTT'AAA

Cuts at:   1540    1540 
   Size:       4541
 
DrdI GACnn_nn'nnGTC

Cuts at:   2247    3105    2247 
   Size:        858    3683
 
EaeI y'GGCC_r

Cuts at:    318    2126    2300    2691    2718    4063     318 
   Size:       1808     174     391      27    1345     796

  Fragments arranged by size: 

                1808    1345     796     391     174      27
 
EagI C'GGCC_G

Cuts at:   2126    2126 
   Size:       4541
 
EarI CTCTTCn'nnn_

Cuts at:    151     925    1223    2564    2774     151 
   Size:        774     298    1341     210    1918

  Fragments arranged by size: 

                1918    1341     774     298     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2749    3059    3205    4351    2749 
   Size:        310     146    1146    2939

  Fragments arranged by size: 

                2939    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1553    1553 
   Size:       4541
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    271     392     496     949    1070    2366    2798    3545    3813 
   Size:        121     104     453     121    1296     432     747     268

Cuts at:   3813     271 
   Size:        999

  Fragments arranged by size: 

                1296     999     747     453     432     268     121     121
                 104
 
EcoNI CCTnn'n_nnAGG

Cuts at:    253     839    1228    1390     253 
   Size:        586     389     162    3404

  Fragments arranged by size: 

                3404     586     389     162
 
EcoO109I rG'GnC_Cy

Cuts at:    183    1680     183 
   Size:       1497    3044
 
EcoRI G'AATT_C

Cuts at:    957    1002    1545     957 
   Size:         45     543    3953

  Fragments arranged by size: 

                3953     543      45
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    703    2560     703 
   Size:       1857    2684
 
FspI TGC'GCA

Cuts at:   2322    2322 
   Size:       4541
 
HaeII r_GCGC'y

Cuts at:    125     204    1975    2223    3245    4224     125 
   Size:         79    1771     248    1022     979     442

  Fragments arranged by size: 

                1771    1022     979     442     248      79
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    865     897     898     930    1269    1301    2383    2413    2415 
   Size:         32       1      32     339      32    1082      30       2

Cuts at:   2415    2445     865 
   Size:         30    2961

  Fragments arranged by size: 

                2961    1082     339      32      32      32      30      30
                   2       1
 
HincII GTy'rAC

Cuts at:    791     890    1580     791 
   Size:         99     690    3752

  Fragments arranged by size: 

                3752     690      99
 
HindIII A'AGCT_T

Cuts at:    818     818 
   Size:       4541
 
KasI G'GCGC_C

Cuts at:    200    1971    2219     200 
   Size:       1771     248    2522

  Fragments arranged by size: 

                2522    1771     248
 
KpnI G_GTAC'C

Cuts at:   1561    1561 
   Size:       4541
 
MluI A'CGCG_T

Cuts at:   3974    3974 
   Size:       4541
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    689     876    3212    3396     689 
   Size:        187    2336     184    1834

  Fragments arranged by size: 

                2336    1834     187     184
 
MscI TGG'CCA

Cuts at:    320    2302     320 
   Size:       1982    2559
 
MslI CAynn'nnrTG

Cuts at:   2530    2657    2530 
   Size:        127    4414
 
MspA1I CmG'CkG

Cuts at:    312    1652    1966    2326    3339    3584     312 
   Size:       1340     314     360    1013     245    1269

  Fragments arranged by size: 

                1340    1269    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2722    2722 
   Size:       4541
 
NarI GG'CG_CC

Cuts at:    201    1972    2220     201 
   Size:       1771     248    2522

  Fragments arranged by size: 

                2522    1771     248
 
NgoMIV G'CCGG_C

Cuts at:   2720    2720 
   Size:       4541
 
NspI r_CATG'y

Cuts at:   1594    2094    3001    1594 
   Size:        500     907    3134

  Fragments arranged by size: 

                3134     907     500
 
PciI A'CATG_T

Cuts at:   2997    2997 
   Size:       4541
 
PmeI GTTT'AAAC

Cuts at:   1540    1540 
   Size:       4541
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3680    3712    3680 
   Size:         32    4509
 
PpuMI rG'GwC_Cy

Cuts at:    183     183 
   Size:       4541
 
PstI C_TGCA'G

Cuts at:   1358    1588    2273    1358 
   Size:        230     685    3626

  Fragments arranged by size: 

                3626     685     230
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4541
 
PvuII CAG'CTG

Cuts at:   1966    2326    1966 
   Size:        360    4181
 
RsrII CG'GwC_CG

Cuts at:   2736    2736 
   Size:       4541
 
SacI G_AGCT'C

Cuts at:   1555    1555 
   Size:       4541
 
SacII CC_GC'GG

Cuts at:    313     313 
   Size:       4541
 
SalI G'TCGA_C

Cuts at:   1578    1578 
   Size:       4541
 
SanDI GG'GwC_CC

Cuts at:    183     183 
   Size:       4541
 
SapI GCTCTTCn'nnn_

Cuts at:    151    2564    2774     151 
   Size:       2413     210    1918

  Fragments arranged by size: 

                2413    1918     210
 
SbfI CC_TGCA'GG

Cuts at:   1588    1588 
   Size:       4541
 
ScaI AGT'ACT

Cuts at:   4058    4058 
   Size:       4541
 
SfcI C'TryA_G

Cuts at:     33    1250    1354    1584    2269    3262    3453      33 
   Size:       1217     104     230     685     993     191    1121

  Fragments arranged by size: 

                1217    1121     993     685     230     191     104
 
SfoI GGC'GCC

Cuts at:    202    1973    2221     202 
   Size:       1771     248    2522

  Fragments arranged by size: 

                2522    1771     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4541
 
SmaI CCC'GGG

Cuts at:   1563    1563 
   Size:       4541
 
SmlI C'TyrA_G

Cuts at:   1406    1696    2611    3103    3365    1406 
   Size:        290     915     492     262    2582

  Fragments arranged by size: 

                2582     915     492     290     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4541
 
SphI G_CATG'C

Cuts at:   1594    2094    1594 
   Size:        500    4041
 
StuI AGG'CCT

Cuts at:    804     804 
   Size:       4541
 
StyI C'CwwG_G

Cuts at:   1459    1675    2029    1459 
   Size:        216     354    3971

  Fragments arranged by size: 

                3971     354     216
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2124    2257    2716    2925    2124 
   Size:        133     459     209    3740

  Fragments arranged by size: 

                3740     459     209     133
 
TatI w'GTAC_w

Cuts at:    612    4056     612 
   Size:       3444    1097
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4058    4090    4058 
   Size:         32    4509
 
Tth111I GACn'n_nGTC

Cuts at:   2338    2338 
   Size:       4541
 
XbaI T'CTAG_A

Cuts at:   1572    1572 
   Size:       4541
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2036    2036 
   Size:       4541
 
XmaI C'CCGG_G

Cuts at:   1561    1561 
   Size:       4541
 
XmnI GAAnn'nnTTC

Cuts at:   1016    1016 
   Size:       4541
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PciI     PmeI     PpiI    PpuMI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BcgI    BfrBI     BglI     BmtI    BsaXI
     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI     HpaI
     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XhoI     ZraI
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