Restriction map for pF1KA1154
 (Linear) (Six-base) MAPSORT of: KIAA1154.seq  Check: 6172  from: 1  to: 1428



KIAA1154.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AccI GT'mk_AC

Cuts at:      0    1040    1334    1428 
   Size:       1040     294      94

  Fragments arranged by size: 

                1040     294      94
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     388     841    1428 
   Size:        163     225     453     587

  Fragments arranged by size: 

                 587     453     225     163
 
AflIII A'CryG_T

Cuts at:      0     377    1428 
   Size:        377    1051
 
AlwNI CAG_nnn'CTG

Cuts at:      0     819    1428 
   Size:        819     609
 
ApaLI G'TGCA_C

Cuts at:      0     414    1428 
   Size:        414    1014
 
ApoI r'AATT_y

Cuts at:      0     849     894    1428 
   Size:        849      45     534

  Fragments arranged by size: 

                 849     534      45
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     986    1019    1428 
   Size:        986      33     409

  Fragments arranged by size: 

                 986     409      33
 
BamHI G'GATC_C

Cuts at:      0     736     790    1428 
   Size:        736      54     638

  Fragments arranged by size: 

                 736     638      54
 
BanI G'GyrC_C

Cuts at:      0      92     168    1219    1428 
   Size:         92      76    1051     209

  Fragments arranged by size: 

                1051     209      92      76
 
BbeI G_GCGC'C

Cuts at:      0      96    1428 
   Size:         96    1332
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     129     848    1097    1428 
   Size:        129     719     249     331

  Fragments arranged by size: 

                 719     331     249     129
 
BbvCI CC'TCA_GC

Cuts at:      0     812    1428 
   Size:        812     616
 
Bme1580I G_kGCm'C

Cuts at:      0     418    1428 
   Size:        418    1010
 
BmgBI CAC'GTC

Cuts at:      0     846    1428 
   Size:        846     582
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     962    1428 
   Size:        284     678     466

  Fragments arranged by size: 

                 678     466     284
 
Bpu10I CC'TnA_GC

Cuts at:      0     812    1154    1428 
   Size:        812     342     274

  Fragments arranged by size: 

                 812     342     274
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1283    1428 
   Size:       1283     145
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     434    1428 
   Size:        434     994
 
BsaAI yAC'GTr

Cuts at:      0     258    1428 
   Size:        258    1170
 
BsaHI Gr'CG_yC

Cuts at:      0      93    1428 
   Size:         93    1335
 
BsaWI w'CCGG_w

Cuts at:      0     221    1428 
   Size:        221    1207
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     552     800    1180    1243    1255    1428 
   Size:        552     248     380      63      12     173

  Fragments arranged by size: 

                 552     380     248     173      63      12
 
BseYI C'CCAG_C

Cuts at:      0     453    1428 
   Size:        453     975
 
BsiHKAI G_wGCw'C

Cuts at:      0     418    1428 
   Size:        418    1010
 
Bsp1286I G_dGCh'C

Cuts at:      0     418    1428 
   Size:        418    1010
 
BspEI T'CCGG_A

Cuts at:      0     221    1428 
   Size:        221    1207
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1242    1428 
   Size:       1242     186
 
BsrBI CCG'CTC

Cuts at:      0       5     245     542    1428 
   Size:          5     240     297     886

  Fragments arranged by size: 

                 886     297     240       5
 
BsrFI r'CCGG_y

Cuts at:      0     154    1428 
   Size:        154    1274
 
BssSI C'ACGA_G

Cuts at:      0    1205    1428 
   Size:       1205     223
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1201    1428 
   Size:       1201     227
 
BstBI TT'CG_AA

Cuts at:      0     131    1428 
   Size:        131    1297
 
BstEII G'GTnAC_C

Cuts at:      0     150    1111    1428 
   Size:        150     961     317

  Fragments arranged by size: 

                 961     317     150
 
BstYI r'GATC_y

Cuts at:      0     236     736     790    1428 
   Size:        236     500      54     638

  Fragments arranged by size: 

                 638     500     236      54
 
BtgI C'CryG_G

Cuts at:      0     202    1421    1428 
   Size:        202    1219       7

  Fragments arranged by size: 

                1219     202       7
 
BtsI GCAGTG_nn'

Cuts at:      0    1317    1428 
   Size:       1317     111
 
EaeI y'GGCC_r

Cuts at:      0     210    1428 
   Size:        210    1218
 
EarI CTCTTCn'nnn_

Cuts at:      0      43     817    1115    1428 
   Size:         43     774     298     313

  Fragments arranged by size: 

                 774     313     298      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     284     388     841     962    1428 
   Size:        163     121     104     453     121     466

  Fragments arranged by size: 

                 466     453     163     121     121     104
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     145     731    1120    1282    1428 
   Size:        145     586     389     162     146

  Fragments arranged by size: 

                 586     389     162     146     145
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75    1428 
   Size:         75    1353
 
EcoRI G'AATT_C

Cuts at:      0     849     894    1428 
   Size:        849      45     534

  Fragments arranged by size: 

                 849     534      45
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     595    1428 
   Size:        595     833
 
HaeII r_GCGC'y

Cuts at:      0      17      96    1428 
   Size:         17      79    1332

  Fragments arranged by size: 

                1332      79      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     757     789     790     822    1161    1193    1428 
   Size:        757      32       1      32     339      32     235

  Fragments arranged by size: 

                 757     339     235      32      32      32       1
 
HincII GTy'rAC

Cuts at:      0     683     782    1428 
   Size:        683      99     646

  Fragments arranged by size: 

                 683     646      99
 
HindIII A'AGCT_T

Cuts at:      0     710    1428 
   Size:        710     718
 
KasI G'GCGC_C

Cuts at:      0      92    1428 
   Size:         92    1336
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     581     768    1428 
   Size:        581     187     660

  Fragments arranged by size: 

                 660     581     187
 
MscI TGG'CCA

Cuts at:      0     212    1428 
   Size:        212    1216
 
MspA1I CmG'CkG

Cuts at:      0     204    1428 
   Size:        204    1224
 
NarI GG'CG_CC

Cuts at:      0      93    1428 
   Size:         93    1335
 
PpuMI rG'GwC_Cy

Cuts at:      0      75    1428 
   Size:         75    1353
 
PstI C_TGCA'G

Cuts at:      0    1250    1428 
   Size:       1250     178
 
SacII CC_GC'GG

Cuts at:      0     205    1428 
   Size:        205    1223
 
SanDI GG'GwC_CC

Cuts at:      0      75    1428 
   Size:         75    1353
 
SapI GCTCTTCn'nnn_

Cuts at:      0      43    1428 
   Size:         43    1385
 
SfcI C'TryA_G

Cuts at:      0    1142    1246    1428 
   Size:       1142     104     182

  Fragments arranged by size: 

                1142     182     104
 
SfoI GGC'GCC

Cuts at:      0      94    1428 
   Size:         94    1334
 
SmlI C'TyrA_G

Cuts at:      0    1298    1428 
   Size:       1298     130
 
StuI AGG'CCT

Cuts at:      0     696    1428 
   Size:        696     732
 
StyI C'CwwG_G

Cuts at:      0    1351    1428 
   Size:       1351      77
 
TatI w'GTAC_w

Cuts at:      0     504    1428 
   Size:        504     924
 
XmnI GAAnn'nnTTC

Cuts at:      0     908    1428 
   Size:        908     520
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI    ApaLI     ApoI     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI Bme1580I    BmgBI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstBI
   BstEII    BstYI     BtgI     BtsI     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII     KasI
     MmeI     MscI   MspA1I     NarI    PpuMI     PstI    SacII    SanDI
     SapI     SfcI     SfoI     SmlI     StuI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII    BanII     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BmtI     BplI    BsaBI    BsaXI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspHI    BsrDI    BsrGI   BssHII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI     HpaI
     KpnI     MfeI     MluI     MslI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
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