Restriction map for pF1KA1147
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

Acc65I      AccIII      AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrDI       BsrGI       BssSI       Bst1107I    BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        MfeI        MluI        MroI        MseI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SfiI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tru9I       Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII         994  GACGTC               GACGT^C
AclI          844  AACGTT               AA^CGTT
AcsI          751  AAATTT               [GA]^AATT[CT]
AflIII        662  ACATGT               A^C[GA][CT]GT
AlwI          735  GATCC                GGATC(4/5)
ApoI          751  AAATTT               [GA]^AATT[CT]
Asp700I       963  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         592  GTGCTC               G[AT]GC[AT]^C
BsaI         1286  GAGACC               GGTCTC(1/5)
BsgI          951  CTGCAC               GTGCAG(16/14)
BsiHKAI       592  GTGCTC               G[AT]GC[AT]^C
BspLU11I      662  ACATGT               A^CATGT
BsrBI         261  CCGCTC               CCGCTC(-3/-3)
BssHII        443  GCGCGC               G^CGCGC
BstBI         764  TTCGAA               TT^CGAA
BstEII        246  GGTCACC              G^GT[ACGT]ACC
BstPI         246  GGTCACC              G^GT[ACGT]ACC
BstYI         734  AGATCC               [GA]^GATC[CT]
EcoO65I       246  GGTCACC              G^GT[ACGT]ACC
EcoT14I      1281  CCAAGG               C^C[AT][AT]GG
FseI          122  GGCCGGCC             GGCCGG^CC
HgaI           16  GACGC                GACGC(5/10)
HinfI         880  GAGTC                G^A[ACGT]TC
HphI          248  TCACC                GGTGA(8/7)
KspI          128  CCGCGG               CCGC^GG
MaeIII        247  GTCAC                ^GT[ACGT]AC
MflI          734  AGATCC               [GA]^GATC[CT]
MslI          414  CATGCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV          764  TTCGAA               TT^CGAA
PflMI         225  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          880  GAGTC                GAGTC(4/5)
Psp1406I      844  AACGTT               AA^CGTT
PvuII         692  CAGCTG               CAG^CTG
SacII         128  CCGCGG               CCGC^GG
SfuI          764  TTCGAA               TT^CGAA
SmlI          293  CTCAAG               C^T[CT][GA]AG
SphI         1254  GCATGC               GCATG^C
StuI         1316  AGGCCT               AGG^CCT
StyI         1281  CCAAGG               C^C[AT][AT]GG
Tsp45I        247  GTCAC                ^GT[GC]AC
Tsp509I       752  AATT                 ^AATT
Van91I        225  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          410  CCAACATGCCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         734  AGATCC               [GA]^GATC[CT]
XmnI          963  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          634  GTCTAC               GT^[AC][GT]AC
              997  GTCTAC              
AlwNI        1042  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1105  CAGATGCTG           
ApaI           45  GGGCCC               GGGCC^C
              382  GGGCCC              
BalI          332  TGGCCA               TGG^CCA
              839  TGGCCA              
BbsI         1020  GAAGAC               GAAGAC(2/6)
             1135  GAAGAC              
BfaI         1180  CTAG                 C^TAG
             1273  CTAG                
BpuAI        1020  GAAGAC               GAAGAC(2/6)
             1135  GAAGAC              
BsaAI         898  TACGTG               [CT]AC^GT[GA]
             1000  TACGTG              
BsaBI         675  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              735  GATCCTCATC          
BsaWI        1193  ACCGGA               [AT]^CCGG[AT]
             1289  ACCGGA              
BsmBI          54  CGTCTC               CGTCTC(1/5)
               14  GAGACG              
BsmFI         342  GTCCC                GGGAC(10/14)
              605  GTCCC               
BspMI        1037  ACCTGC               ACCTGC(4/8)
             1307  ACCTGC              
BstAPI       1041  GCAGCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1249  GCAGAGCATGC         
DsaI          128  CCGCGG               C^C[GA][CT]GG
              419  CCGTGG              
EarI         1162  CTCTTC               CTCTTC(1/4)
             1153  GAAGAG              
EcoNI          60  CCTGCCGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1308  CCTGCTGGAGG         
Ksp632I      1162  CTCTTC               CTCTTC(1/4)
             1153  GAAGAG              
MaeI         1180  CTAG                 C^TAG
             1273  CTAG                
MamI          675  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              735  GATCCTCATC          
MluNI         332  TGGCCA               TGG^CCA
              839  TGGCCA              
MscI          332  TGGCCA               TGG^CCA
              839  TGGCCA              
PpuMI         340  GGGTCCC              [GA]G^G[AT]CC[CT]
             1157  AGGACCT             
PstI          184  CTGCAG               CTGCA^G
             1039  CTGCAG              
SexAI         224  ACCAGGT              A^CC[AT]GGT
              518  ACCAGGT             
SmaI          190  CCCGGG               CCC^GGG
             1259  CCCGGG              
XmaCI         190  CCCGGG               C^CCGGG
             1259  CCCGGG              
XmaI          190  CCCGGG               C^CCGGG
             1259  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaI        AvaII       BanI        BanII       BbeI        
BbvI        BcnI        BglI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsiEI       BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EagI        EclXI       
Eco52I      EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HpaII       ItaI        KasI        
MaeII       MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        TaqI        
TseI        TspRI       TthHB8I     
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