Restriction map for pF1KA1147
 (Circular) (Six-base) MAPSORT of: pF1KA1147.seq  Check: 5599  from: 1  to: 4478



pF1KA1147  4478 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1154    1154 
   Size:       4478
 
AatII G_ACGT'C

Cuts at:   1106    1106 
   Size:       4478
 
AccI GT'mk_AC

Cuts at:    743    1106    1516     743 
   Size:        363     410    3705

  Fragments arranged by size: 

                3705     410     363
 
Acc65I G'GTAC_C

Cuts at:   1494    1494 
   Size:       4478
 
AclI AA'CG_TT

Cuts at:    953    4318     953 
   Size:       3365    1113
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1180    2303    2735    3088    3750    1180 
   Size:       1123     432     353     662    1908

  Fragments arranged by size: 

                1908    1123     662     432     353
 
AflIII A'CryG_T

Cuts at:    770    1830    3630    3911     770 
   Size:       1060    1800     281    1337

  Fragments arranged by size: 

                1800    1337    1060     281
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    748     748 
   Size:       4478
 
AlwNI CAG_nnn'CTG

Cuts at:   1155    1218    1783    3221    1155 
   Size:         63     565    1438    2412

  Fragments arranged by size: 

                2412    1438     565      63
 
ApaI G_GGCC'C

Cuts at:    157     494     157 
   Size:        337    4141
 
ApaLI G'TGCA_C

Cuts at:   3316    3316 
   Size:       4478
 
ApoI r'AATT_y

Cuts at:    859    1482    3831    4087     859 
   Size:        623    2349     256    1250

  Fragments arranged by size: 

                2349    1250     623     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4478
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4478
 
AvaI C'yCGr_G

Cuts at:    262     298     372    1367    1498     262 
   Size:         36      74     995     131    3242

  Fragments arranged by size: 

                3242     995     131      74      36
 
BamHI G'GATC_C

Cuts at:   1503    1503 
   Size:       4478
 
BanI G'GyrC_C

Cuts at:    129     223     302     635     976    1494    1908    2156    2191 
   Size:         94      79     333     341     518     414     248      35

Cuts at:   2191     129 
   Size:       2416

  Fragments arranged by size: 

                2416     518     414     341     333     248      94      79
                  35
 
BanII G_rGCy'C

Cuts at:    157     263     494    1492    2522     157 
   Size:        106     231     998    1030    2113

  Fragments arranged by size: 

                2113    1030     998     231     106
 
BbeI G_GCGC'C

Cuts at:    133     227     306    1912    2160     133 
   Size:         94      79    1606     248    2451

  Fragments arranged by size: 

                2451    1606     248      94      79
 
BbsI GAAGACnn'nnnn_

Cuts at:   1135    1250    1135 
   Size:        115    4363
 
BciVI GTATCCnnnnn_n'

Cuts at:   2376    3432    4478    2376 
   Size:       1056    1046    2376

  Fragments arranged by size: 

                2376    1056    1046
 
BclI T'GATC_A

Cuts at:   1998    1998 
   Size:       4478
 
BglI GCCn_nnn'nGGC

Cuts at:    212     232     252     212 
   Size:         20      20    4438

  Fragments arranged by size: 

                4438      20      20
 
BglII A'GATC_T

Cuts at:   1993    4030    1993 
   Size:       2037    2441
 
BlpI GC'TnA_GC

Cuts at:   1590    1590 
   Size:       4478
 
Bme1580I G_kGCm'C

Cuts at:    157     494     979    2103    2196    3320     157 
   Size:        337     485    1124      93    1124    1315

  Fragments arranged by size: 

                1315    1124    1124     485     337      93
 
BmgBI CAC'GTC

Cuts at:    321     321 
   Size:       4478
 
BmrI ACTGGGnnnn_n'

Cuts at:   1690    1771    2106    4250    1690 
   Size:         81     335    2144    1918

  Fragments arranged by size: 

                2144    1918     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2845    2845 
   Size:       4478
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    328    1054    1441     328 
   Size:        726     387    3365

  Fragments arranged by size: 

                3365     726     387
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    386    1654    2533    3247    3545     386 
   Size:       1268     879     714     298    1319

  Fragments arranged by size: 

                1319    1268     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1388    4182    1388 
   Size:       2794    1684
 
BsaAI yAC'GTr

Cuts at:   1008    1110    1831    1008 
   Size:        102     721    3655

  Fragments arranged by size: 

                3655     721     102
 
BsaBI GATnn'nnATC

Cuts at:      6     787     847    1992    4029       6 
   Size:        781      60    1145    2037     455

  Fragments arranged by size: 

                2037    1145     781     455      60
 
BsaHI Gr'CG_yC

Cuts at:    130     224     303    1103    1909    2157    2859    4355     130 
   Size:         94      79     800     806     248     702    1496     253

  Fragments arranged by size: 

                1496     806     800     702     253     248      94      79
            
 
BsaWI w'CCGG_w

Cuts at:   1301    1397    1667    2188    2866    3277    3424    3698    1301 
   Size:         96     270     521     678     411     147     274    2081

  Fragments arranged by size: 

                2081     678     521     411     274     270     147      96
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    280     340    1096     280 
   Size:         60     756    3662

  Fragments arranged by size: 

                3662     756      60
 
BseYI C'CCAG_C

Cuts at:    143     200     312     798    1903    2661    3326    3734     143 
   Size:         57     112     486    1105     758     665     408     887

  Fragments arranged by size: 

                1105     887     758     665     486     408     112      57
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1044    1044 
   Size:       4478
 
BsiEI CG_ry'CG

Cuts at:    104     232     236     275    2020    2066    3296     104 
   Size:        128       4      39    1745      46    1230    1286

  Fragments arranged by size: 

                1745    1286    1230     128      46      39       4
 
BsiHKAI G_wGCw'C

Cuts at:    704    1492    2270    2460    3320     704 
   Size:        788     778     190     860    1862

  Fragments arranged by size: 

                1862     860     788     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    116     168     116 
   Size:         52    4426
 
Bsp1286I G_dGCh'C

Cuts at:    157     263     494     704     979    1492    2103    2196    2270 
   Size:        106     231     210     275     513     611      93      74

Cuts at:   2270    2460    2522    3320     157 
   Size:        190      62     798    1315

  Fragments arranged by size: 

                1315     798     611     513     275     231     210     190
                 106      93      74      62
 
BspHI T'CATG_A

Cuts at:   2910    2910 
   Size:       4478
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1154    1424    1528    1742    2044    2425    1154 
   Size:        270     104     214     302     381    3207

  Fragments arranged by size: 

                3207     381     302     270     214     104
 
BsrBI CCG'CTC

Cuts at:     50     371    2770    2824    4308    4473      50 
   Size:        321    2399      54    1484     165      55

  Fragments arranged by size: 

                2399    1484     321     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2390    2390 
   Size:       4478
 
BsrFI r'CCGG_y

Cuts at:    231     270    1193    2476    2657     231 
   Size:         39     923    1283     181    2052

  Fragments arranged by size: 

                2052    1283     923     181      39
 
BssHII G'CGCG_C

Cuts at:    551     551 
   Size:       4478
 
BssSI C'ACGA_G

Cuts at:   2749    3457    2749 
   Size:        708    3770
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1155    1363    1855    1155 
   Size:        208     492    3778

  Fragments arranged by size: 

                3778     492     208
 
BstBI TT'CG_AA

Cuts at:    873    2839     873 
   Size:       1966    2512
 
BstEII G'GTnAC_C

Cuts at:    354     354 
   Size:       4478
 
BstYI r'GATC_y

Cuts at:      1     842    1503    1970    1993    2328    2574    2880    2892 
   Size:        841     661     467      23     335     246     306      12

Cuts at:   2892    2978    4030       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1052     841     661     467     449     335     306     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     236     527     107 
   Size:        129     291    4058

  Fragments arranged by size: 

                4058     291     129
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2428    2609    2772    2428 
   Size:        181     163    4134

  Fragments arranged by size: 

                4134     181     163
 
BtsI GCAGTG_nn'

Cuts at:    592     958    1721     592 
   Size:        366     763    3349

  Fragments arranged by size: 

                3349     763     366
 
DraI TTT'AAA

Cuts at:   1477    1477 
   Size:       4478
 
DrdI GACnn_nn'nnGTC

Cuts at:   2184    3528    2184 
   Size:       1344    3134
 
EaeI y'GGCC_r

Cuts at:    229     233     272     341     440     947    2063    2237    2628 
   Size:          4      39      69      99     507    1116     174     391

Cuts at:   2628    2655    4000     229 
   Size:         27    1345     707

  Fragments arranged by size: 

                1345    1116     707     507     391     174      99      69
                  39      27       4
 
EagI C'GGCC_G

Cuts at:    229     233     272    2063     229 
   Size:          4      39    1791    2644

  Fragments arranged by size: 

                2644    1791      39       4
 
EarI CTCTTCn'nnn_

Cuts at:   1256    1276    2501    2711    1256 
   Size:         20    1225     210    3023

  Fragments arranged by size: 

                3023    1225     210      20
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2686    3428    3574    4288    2686 
   Size:        742     146     714    2876

  Fragments arranged by size: 

                2876     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1490    1490 
   Size:       4478
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    328    1054    1180    1441    2303    2735    3088    3750     328 
   Size:        726     126     261     862     432     353     662    1056

  Fragments arranged by size: 

                1056     862     726     662     432     353     261     126
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    172    1420     172 
   Size:       1248    3230
 
EcoO109I rG'GnC_Cy

Cuts at:    153     154     177     449     490     491    1266    1617     153 
   Size:          1      23     272      41       1     775     351    3014

  Fragments arranged by size: 

                3014     775     351     272      41      23       1       1
            
 
EcoRI G'AATT_C

Cuts at:   1482    1482 
   Size:       4478
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    510    2497     510 
   Size:       1987    2491
 
FseI GG_CCGG'CC

Cuts at:    235     235 
   Size:       4478
 
FspI TGC'GCA

Cuts at:   2259    2259 
   Size:       4478
 
HaeII r_GCGC'y

Cuts at:    133     227     306    1912    2160    3390    4161     133 
   Size:         94      79    1606     248    1230     771     450

  Fragments arranged by size: 

                1606    1230     771     450     248      94      79
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    355     387     418     450     827     859     879     911    2320 
   Size:         32      31      32     377      32      20      32    1409

Cuts at:   2320    2350    2352    2382     355 
   Size:         30       2      30    2451

  Fragments arranged by size: 

                2451    1409     377      32      32      32      32      31
                  30      30      20       2
 
HincII GTy'rAC

Cuts at:   1517    1517 
   Size:       4478
 
KasI G'GCGC_C

Cuts at:    129     223     302    1908    2156     129 
   Size:         94      79    1606     248    2451

  Fragments arranged by size: 

                2451    1606     248      94      79
 
KpnI G_GTAC'C

Cuts at:   1498    1498 
   Size:       4478
 
MluI A'CGCG_T

Cuts at:   3911    3911 
   Size:       4478
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    623    1301    3237    3421     623 
   Size:        678    1936     184    1680

  Fragments arranged by size: 

                1936    1680     678     184
 
MscI TGG'CCA

Cuts at:    442     949    2239     442 
   Size:        507    1290    2681

  Fragments arranged by size: 

                2681    1290     507
 
MslI CAynn'nnrTG

Cuts at:    526    2467    2594     526 
   Size:       1941     127    2410

  Fragments arranged by size: 

                2410    1941     127
 
MspA1I CmG'CkG

Cuts at:    135     238     802    1155    1589    1903    2263    3047    3292 
   Size:        103     564     353     434     314     360     784     245

Cuts at:   3292     135 
   Size:       1321

  Fragments arranged by size: 

                1321     784     564     434     360     353     314     245
                 103
 
NaeI GCC'GGC

Cuts at:    233     272    1195    2659     233 
   Size:         39     923    1464    2052

  Fragments arranged by size: 

                2052    1464     923      39
 
NarI GG'CG_CC

Cuts at:    130     224     303    1909    2157     130 
   Size:         94      79    1606     248    2451

  Fragments arranged by size: 

                2451    1606     248      94      79
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4478
 
NgoMIV G'CCGG_C

Cuts at:    231     270    1193    2657     231 
   Size:         39     923    1464    2052

  Fragments arranged by size: 

                2052    1464     923      39
 
NspI r_CATG'y

Cuts at:    525     612     774     813    1366    1531    2031    3634     525 
   Size:         87     162      39     553     165     500    1603    1369

  Fragments arranged by size: 

                1603    1369     553     500     165     162      87      39
            
 
PciI A'CATG_T

Cuts at:    770    3630     770 
   Size:       2860    1618
 
PflMI CCAn_nnn'nTGG

Cuts at:    339     339 
   Size:       4478
 
PfoI T'CCnGG_A

Cuts at:   1409    1409 
   Size:       4478
 
PmeI GTTT'AAAC

Cuts at:   1477    1477 
   Size:       4478
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    830     862    2924    2956     830 
   Size:         32    2062      32    2352

  Fragments arranged by size: 

                2352    2062      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    449    1266     449 
   Size:        817    3661
 
PsiI TTA'TAA

Cuts at:    823     823 
   Size:       4478
 
PspOMI G'GGCC_C

Cuts at:    153     490     153 
   Size:        337    4141
 
PstI C_TGCA'G

Cuts at:    296    1151    1525    2210     296 
   Size:        855     374     685    2564

  Fragments arranged by size: 

                2564     855     685     374
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4478
 
PvuII CAG'CTG

Cuts at:    802    1903    2263     802 
   Size:       1101     360    3017

  Fragments arranged by size: 

                3017    1101     360
 
RsrII CG'GwC_CG

Cuts at:   2673    2673 
   Size:       4478
 
SacI G_AGCT'C

Cuts at:   1492    1492 
   Size:       4478
 
SacII CC_GC'GG

Cuts at:    239     239 
   Size:       4478
 
SalI G'TCGA_C

Cuts at:   1515    1515 
   Size:       4478
 
SanDI GG'GwC_CC

Cuts at:    449     449 
   Size:       4478
 
SapI GCTCTTCn'nnn_

Cuts at:   2501    2711    2501 
   Size:        210    4268
 
SbfI CC_TGCA'GG

Cuts at:   1525    1525 
   Size:       4478
 
ScaI AGT'ACT

Cuts at:   3995    3995 
   Size:       4478
 
SexAI A'CCwGG_T

Cuts at:    332     626     332 
   Size:        294    4184
 
SfcI C'TryA_G

Cuts at:     33     292     921    1147    1521    2206    3174    3365      33 
   Size:        259     629     226     374     685     968     191    1146

  Fragments arranged by size: 

                1146     968     685     629     374     259     226     191
            
 
SfoI GGC'GCC

Cuts at:    131     225     304    1910    2158     131 
   Size:         94      79    1606     248    2451

  Fragments arranged by size: 

                2451    1606     248      94      79
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4478
 
SmaI CCC'GGG

Cuts at:    300    1369    1500     300 
   Size:       1069     131    3278

  Fragments arranged by size: 

                3278    1069     131
 
SmlI C'TyrA_G

Cuts at:    401    1633    2548    3262    3524     401 
   Size:       1232     915     714     262    1355

  Fragments arranged by size: 

                1355    1232     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4478
 
SphI G_CATG'C

Cuts at:   1366    1531    2031    1366 
   Size:        165     500    3813

  Fragments arranged by size: 

                3813     500     165
 
SrfI GCCC'GGGC

Cuts at:    300     300 
   Size:       4478
 
StuI AGG'CCT

Cuts at:   1426    1426 
   Size:       4478
 
StyI C'CwwG_G

Cuts at:    107    1389    1612    1966     107 
   Size:       1282     223     354    2619

  Fragments arranged by size: 

                2619    1282     354     223
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    812    2061    2194    2653    2862     812 
   Size:       1249     133     459     209    2428

  Fragments arranged by size: 

                2428    1249     459     209     133
 
TatI w'GTAC_w

Cuts at:    929    1223    3993     929 
   Size:        294    2770    1414

  Fragments arranged by size: 

                2770    1414     294
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3995    4027    3995 
   Size:         32    4446
 
Tth111I GACn'n_nGTC

Cuts at:   2275    2275 
   Size:       4478
 
XbaI T'CTAG_A

Cuts at:   1509    1509 
   Size:       4478
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    525    1973     525 
   Size:       1448    3030
 
XmaI C'CCGG_G

Cuts at:    298    1367    1498     298 
   Size:       1069     131    3278

  Fragments arranged by size: 

                3278    1069     131
 
XmnI GAAnn'nnTTC

Cuts at:   1075    1075 
   Size:       4478
 
ZraI GAC'GTC

Cuts at:   1104    1104 
   Size:       4478
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI     PsiI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AgeI     AhdI     AleI     AloI     AscI    AvrII
     BaeI    BbvCI     BcgI    BfrBI     BmtI   Bpu10I    BsaXI    BsiWI
     BsmI    BspEI    BsrGI    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoRV    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PmlI    PshAI    PspXI
     PsrI     SfiI    SgrAI    SnaBI     SspI     SwaI     XhoI
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