Restriction map for pF1KA1147
 (Linear) (Six-base) MAPSORT of: KIAA1147.seq  Check: 5017  from: 1  to: 1365



KIAA1147.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1046    1365 
   Size:       1046     319
 
AatII G_ACGT'C

Cuts at:      0     998    1365 
   Size:        998     367
 
AccI GT'mk_AC

Cuts at:      0     635     998    1365 
   Size:        635     363     367

  Fragments arranged by size: 

                 635     367     363
 
AclI AA'CG_TT

Cuts at:      0     845    1365 
   Size:        845     520
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1072    1365 
   Size:       1072     293
 
AflIII A'CryG_T

Cuts at:      0     662    1365 
   Size:        662     703
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     640    1365 
   Size:        640     725
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1047    1110    1365 
   Size:       1047      63     255

  Fragments arranged by size: 

                1047     255      63
 
ApaI G_GGCC'C

Cuts at:      0      49     386    1365 
   Size:         49     337     979

  Fragments arranged by size: 

                 979     337      49
 
ApoI r'AATT_y

Cuts at:      0     751    1365 
   Size:        751     614
 
AvaI C'yCGr_G

Cuts at:      0     154     190     264    1259    1365 
   Size:        154      36      74     995     106

  Fragments arranged by size: 

                 995     154     106      74      36
 
BanI G'GyrC_C

Cuts at:      0      21     115     194     527     868    1365 
   Size:         21      94      79     333     341     497

  Fragments arranged by size: 

                 497     341     333      94      79      21
 
BanII G_rGCy'C

Cuts at:      0      49     155     386    1365 
   Size:         49     106     231     979

  Fragments arranged by size: 

                 979     231     106      49
 
BbeI G_GCGC'C

Cuts at:      0      25     119     198    1365 
   Size:         25      94      79    1167

  Fragments arranged by size: 

                1167      94      79      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1027    1142    1365 
   Size:       1027     115     223

  Fragments arranged by size: 

                1027     223     115
 
BglI GCCn_nnn'nGGC

Cuts at:      0     104     124     144    1365 
   Size:        104      20      20    1221

  Fragments arranged by size: 

                1221     104      20      20
 
Bme1580I G_kGCm'C

Cuts at:      0      49     386     871    1365 
   Size:         49     337     485     494

  Fragments arranged by size: 

                 494     485     337      49
 
BmgBI CAC'GTC

Cuts at:      0     213    1365 
   Size:        213    1152
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     946    1333    1365 
   Size:        220     726     387      32

  Fragments arranged by size: 

                 726     387     220      32
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278    1365 
   Size:        278    1087
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1280    1365 
   Size:       1280      85
 
BsaAI yAC'GTr

Cuts at:      0     900    1002    1365 
   Size:        900     102     363

  Fragments arranged by size: 

                 900     363     102
 
BsaBI GATnn'nnATC

Cuts at:      0     679     739    1365 
   Size:        679      60     626

  Fragments arranged by size: 

                 679     626      60
 
BsaHI Gr'CG_yC

Cuts at:      0      22     116     195     995    1365 
   Size:         22      94      79     800     370

  Fragments arranged by size: 

                 800     370      94      79      22
 
BsaWI w'CCGG_w

Cuts at:      0    1193    1289    1365 
   Size:       1193      96      76

  Fragments arranged by size: 

                1193      96      76
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     232     988    1365 
   Size:        172      60     756     377

  Fragments arranged by size: 

                 756     377     172      60
 
BseYI C'CCAG_C

Cuts at:      0      35      92     204     690    1365 
   Size:         35      57     112     486     675

  Fragments arranged by size: 

                 675     486     112      57      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     936    1365 
   Size:        936     429
 
BsiEI CG_ry'CG

Cuts at:      0     124     128     167    1365 
   Size:        124       4      39    1198

  Fragments arranged by size: 

                1198     124      39       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     596    1365 
   Size:        596     769
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       8      60    1365 
   Size:          8      52    1305

  Fragments arranged by size: 

                1305      52       8
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     155     386     596     871    1365 
   Size:         49     106     231     210     275     494

  Fragments arranged by size: 

                 494     275     231     210     106      49
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1046    1316    1365 
   Size:       1046     270      49

  Fragments arranged by size: 

                1046     270      49
 
BsrBI CCG'CTC

Cuts at:      0     263    1365 
   Size:        263    1102
 
BsrFI r'CCGG_y

Cuts at:      0     123     162    1085    1365 
   Size:        123      39     923     280

  Fragments arranged by size: 

                 923     280     123      39
 
BssHII G'CGCG_C

Cuts at:      0     443    1365 
   Size:        443     922
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1047    1255    1365 
   Size:       1047     208     110

  Fragments arranged by size: 

                1047     208     110
 
BstBI TT'CG_AA

Cuts at:      0     765    1365 
   Size:        765     600
 
BstEII G'GTnAC_C

Cuts at:      0     246    1365 
   Size:        246    1119
 
BstYI r'GATC_y

Cuts at:      0     734    1365 
   Size:        734     631
 
BtgI C'CryG_G

Cuts at:      0     128     419    1365 
   Size:        128     291     946

  Fragments arranged by size: 

                 946     291     128
 
BtsI GCAGTG_nn'

Cuts at:      0     484     850    1365 
   Size:        484     366     515

  Fragments arranged by size: 

                 515     484     366
 
EaeI y'GGCC_r

Cuts at:      0     121     125     164     233     332     839    1365 
   Size:        121       4      39      69      99     507     526

  Fragments arranged by size: 

                 526     507     121      99      69      39       4
 
EagI C'GGCC_G

Cuts at:      0     121     125     164    1365 
   Size:        121       4      39    1201

  Fragments arranged by size: 

                1201     121      39       4
 
EarI CTCTTCn'nnn_

Cuts at:      0    1148    1168    1365 
   Size:       1148      20     197

  Fragments arranged by size: 

                1148     197      20
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     946    1072    1333    1365 
   Size:        220     726     126     261      32

  Fragments arranged by size: 

                 726     261     220     126      32
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      64    1312    1365 
   Size:         64    1248      53

  Fragments arranged by size: 

                1248      64      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45      46      69     341     382     383    1158    1365 
   Size:         45       1      23     272      41       1     775     207

  Fragments arranged by size: 

                 775     272     207      45      41      23       1       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     402    1365 
   Size:        402     963
 
FseI GG_CCGG'CC

Cuts at:      0     127    1365 
   Size:        127    1238
 
HaeII r_GCGC'y

Cuts at:      0      25     119     198    1365 
   Size:         25      94      79    1167

  Fragments arranged by size: 

                1167      94      79      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     247     279     310     342     719     751     771     803 
   Size:        247      32      31      32     377      32      20      32

Cuts at:    803    1365 
   Size:        562

  Fragments arranged by size: 

                 562     377     247      32      32      32      32      31
                  20
 
KasI G'GCGC_C

Cuts at:      0      21     115     194    1365 
   Size:         21      94      79    1171

  Fragments arranged by size: 

                1171      94      79      21
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    1193    1365 
   Size:        515     678     172

  Fragments arranged by size: 

                 678     515     172
 
MscI TGG'CCA

Cuts at:      0     334     841    1365 
   Size:        334     507     524

  Fragments arranged by size: 

                 524     507     334
 
MslI CAynn'nnrTG

Cuts at:      0     418    1365 
   Size:        418     947
 
MspA1I CmG'CkG

Cuts at:      0      27     130     694    1047    1365 
   Size:         27     103     564     353     318

  Fragments arranged by size: 

                 564     353     318     103      27
 
NaeI GCC'GGC

Cuts at:      0     125     164    1087    1365 
   Size:        125      39     923     278

  Fragments arranged by size: 

                 923     278     125      39
 
NarI GG'CG_CC

Cuts at:      0      22     116     195    1365 
   Size:         22      94      79    1170

  Fragments arranged by size: 

                1170      94      79      22
 
NgoMIV G'CCGG_C

Cuts at:      0     123     162    1085    1365 
   Size:        123      39     923     280

  Fragments arranged by size: 

                 923     280     123      39
 
NspI r_CATG'y

Cuts at:      0     417     504     666     705    1258    1365 
   Size:        417      87     162      39     553     107

  Fragments arranged by size: 

                 553     417     162     107      87      39
 
PciI A'CATG_T

Cuts at:      0     662    1365 
   Size:        662     703
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     231    1365 
   Size:        231    1134
 
PfoI T'CCnGG_A

Cuts at:      0    1301    1365 
   Size:       1301      64
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     722     754    1365 
   Size:        722      32     611

  Fragments arranged by size: 

                 722     611      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     341    1158    1365 
   Size:        341     817     207

  Fragments arranged by size: 

                 817     341     207
 
PsiI TTA'TAA

Cuts at:      0     715    1365 
   Size:        715     650
 
PspOMI G'GGCC_C

Cuts at:      0      45     382    1365 
   Size:         45     337     983

  Fragments arranged by size: 

                 983     337      45
 
PstI C_TGCA'G

Cuts at:      0     188    1043    1365 
   Size:        188     855     322

  Fragments arranged by size: 

                 855     322     188
 
PvuII CAG'CTG

Cuts at:      0     694    1365 
   Size:        694     671
 
SacII CC_GC'GG

Cuts at:      0     131    1365 
   Size:        131    1234
 
SanDI GG'GwC_CC

Cuts at:      0     341    1365 
   Size:        341    1024
 
SexAI A'CCwGG_T

Cuts at:      0     224     518    1365 
   Size:        224     294     847

  Fragments arranged by size: 

                 847     294     224
 
SfcI C'TryA_G

Cuts at:      0     184     813    1039    1365 
   Size:        184     629     226     326

  Fragments arranged by size: 

                 629     326     226     184
 
SfoI GGC'GCC

Cuts at:      0      23     117     196    1365 
   Size:         23      94      79    1169

  Fragments arranged by size: 

                1169      94      79      23
 
SmaI CCC'GGG

Cuts at:      0     192    1261    1365 
   Size:        192    1069     104

  Fragments arranged by size: 

                1069     192     104
 
SmlI C'TyrA_G

Cuts at:      0     293    1365 
   Size:        293    1072
 
SphI G_CATG'C

Cuts at:      0    1258    1365 
   Size:       1258     107
 
SrfI GCCC'GGGC

Cuts at:      0     192    1365 
   Size:        192    1173
 
StuI AGG'CCT

Cuts at:      0    1318    1365 
   Size:       1318      47
 
StyI C'CwwG_G

Cuts at:      0    1281    1365 
   Size:       1281      84
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     704    1365 
   Size:        704     661
 
TatI w'GTAC_w

Cuts at:      0     821    1115    1365 
   Size:        821     294     250

  Fragments arranged by size: 

                 821     294     250
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     417    1365 
   Size:        417     948
 
XmaI C'CCGG_G

Cuts at:      0     190    1259    1365 
   Size:        190    1069     106

  Fragments arranged by size: 

                1069     190     106
 
XmnI GAAnn'nnTTC

Cuts at:      0     967    1365 
   Size:        967     398
 
ZraI GAC'GTC

Cuts at:      0     996    1365 
   Size:        996     369
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI   AflIII     AlfI    AlwNI
     ApaI     ApoI     AvaI     BanI    BanII     BbeI     BbsI     BglI
 Bme1580I    BmgBI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstBI   BstEII    BstYI
     BtgI     BtsI     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I
     FalI     FseI    HaeII    Hin4I     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI    PflMI     PfoI
     PpiI    PpuMI     PsiI   PspOMI     PstI    PvuII    SacII    SanDI
    SexAI     SfcI     SfoI     SmaI     SmlI     SphI     SrfI     StuI
     StyI    TaqII     TatI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AfeI    AflII     AgeI     AhdI     AleI     AloI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI     BmrI     BmtI     BplI
   Bpu10I    BsaXI    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrGI
    BssSI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI  EcoICRI    EcoRI    EcoRV     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
    PshAI    PspXI     PsrI     PvuI    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SspI
     SwaI     TstI  Tth111I     XbaI     XhoI
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