Restriction map for pF1KA1141
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BbeI        BbrPI       BcgI        BclI        BfrI        
BglI        BlnI        BsaAI       BsaHI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BssHII      Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CfoI        ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HpaI        
KasI        KpnI        KspI        MluI        MroI        
MspA1I      MvnI        NarI        NheI        NotI        
NruI        NsiI        NspI        PacI        PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        PvuII       RsrII       SacII       
SalI        SbfI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Van91I      XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AflIII         86  ACACGT               A^C[GA][CT]GT
Alw44I       1973  GTGCAC               G^TGCAC
AlwNI        2491  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1973  GTGCAC               G^TGCAC
BalI         1800  TGGCCA               TGG^CCA
BciVI         762  GTATCC               GTATCC(6/5)
BcnI          989  CCCGG                CC^[GC]GG
BlpI         2227  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2227  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1492  GATAACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          235  GAGACC               GGTCTC(1/5)
Bsp1407I     2586  TGTACA               T^GTACA
BsrGI        2586  TGTACA               T^GTACA
BstBI         268  TTCGAA               TT^CGAA
BstXI         162  CCACCCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        2227  GCTGAGC              GC^T[ACGT]AGC
DraII         119  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI         1800  TGGCCA               [CT]^GGCC[GA]
EcoNI         190  CCTCTGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      119  AGGACCT              [GA]G^G[ACGT]CC[CT]
HgaI         1391  GCGTC                GACGC(5/10)
HincII       2531  GTCAAC               GT[CT]^[GA]AC
HindII       2531  GTCAAC               GT[CT]^[GA]AC
MamI         1492  GATAACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          111  CAATTG               C^AATTG
MluNI        1800  TGGCCA               TGG^CCA
MscI         1800  TGGCCA               TGG^CCA
MunI          111  CAATTG               C^AATTG
NaeI         1406  GCCGGC               GCC^GGC
NciI          989  CCCGG                CC^[GC]GG
NcoI          503  CCATGG               C^CATGG
NdeI         1541  CATATG               CA^TATG
NgoMIV       1406  GCCGGC               G^CCGGC
NspV          268  TTCGAA               TT^CGAA
PaeR7I       2562  CTCGAG               C^TCGAG
PpuMI         119  AGGACCT              [GA]G^G[AT]CC[CT]
PstI         1716  CTGCAG               CTGCA^G
SapI         1183  GCTCTTC              GCTCTTC(1/4)
ScaI         1807  AGTACT               AGT^ACT
SexAI         358  ACCTGGT              A^CC[AT]GGT
SfuI          268  TTCGAA               TT^CGAA
SspBI        2586  TGTACA               T^GTACA
XbaI         1840  TCTAGA               T^CTAGA
XhoI         2562  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AclI         2127  AACGTT               AA^CGTT
             2534  AACGTT              
AspI          464  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2038  GACAGAGTC           
AvaI           67  CTCGGG               C^[CT]CG[GA]G
             2562  CTCGAG              
AvaII         120  GGACC                G^G[AT]CC
              135  GGACC               
BanI         1010  GGCACC               G^G[CT][GA]CC
             2555  GGTGCC              
BanII        2139  GAGCTC               G[GA]GC[CT]^C
             2307  GAGCTC              
BbsI          392  GTCTTC               GAAGAC(2/6)
             2044  GTCTTC              
BbvCI        2568  CCTCAGC              CCTCAGC(-5/-2)
                4  GCTGAGG             
BglII         180  AGATCT               A^GATCT
             1646  AGATCT              
BpuAI         392  GTCTTC               GAAGAC(2/6)
             2044  GTCTTC              
BsaWI         740  ACCGGA               [AT]^CCGG[AT]
             2327  ACCGGA              
BseRI         248  CTCCTC               GAGGAG(10/8)
             1198  GAGGAG              
BsoBI          67  CTCGGG               C^[CT]CG[GA]G
             2562  CTCGAG              
BspHI         447  TCATGA               T^CATGA
              906  TCATGA              
BsrFI        1322  ACCGGC               [GA]^CCGG[CT]
             1406  GCCGGC              
BssSI        1597  CACGAG               CACGAG(-5/-1)
             1738  CACGAG              
Cac8I         590  GCCAGC               GC[ACGT]^[ACGT]GC
             1406  GCCGGC              
Cfr10I       1322  ACCGGC               [GA]^CCGG[CT]
             1406  GCCGGC              
DraI         1936  TTTAAA               TTT^AAA
             2020  TTTAAA              
EarI          124  CTCTTC               CTCTTC(1/4)
             1184  CTCTTC              
HindIII       648  AAGCTT               A^AGCTT
             2462  AAGCTT              
Ksp632I       124  CTCTTC               CTCTTC(1/4)
             1184  CTCTTC              
PflFI         464  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2038  GACAGAGTC           
Psp1406I     2127  AACGTT               AA^CGTT
             2534  AACGTT              
RcaI          447  TCATGA               T^CATGA
              906  TCATGA              
SacI         2139  GAGCTC               GAGCT^C
             2307  GAGCTC              
StuI         1313  AGGCCT               AGG^CCT
             2210  AGGCCT              
TaqI          269  TCGA                 T^CGA
             2563  TCGA                
Tth111I       464  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2038  GACAGAGTC           
TthHB8I       269  TCGA                 T^CGA
             2563  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       BbvI        BfaI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cfr13I      DdeI        DpnII       DsaI        EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII       XmnI        
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