Restriction map for pF1KA1141
 (Circular) (Six-base) MAPSORT of: pF1KA1141.seq  Check: 3618  from: 1  to: 5726



pF1KA1141  5726 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AccI GT'mk_AC

Cuts at:   2764    2764 
   Size:       5726
 
Acc65I G'GTAC_C

Cuts at:   2742    2742 
   Size:       5726
 
AclI AA'CG_TT

Cuts at:   2236    2643    5566    2236 
   Size:        407    2923    2396

  Fragments arranged by size: 

                2923    2396     407
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    744    1326    1717    1999    2055    3551    3983    4730    4998 
   Size:        582     391     282      56    1496     432     747     268

Cuts at:   4998     744 
   Size:       1472

  Fragments arranged by size: 

                1496    1472     747     582     432     391     282     268
                  56
 
AflIII A'CryG_T

Cuts at:    194    3078    4182    5159     194 
   Size:       2884    1104     977     761

  Fragments arranged by size: 

                2884    1104     977     761
 
AgeI A'CCGG_T

Cuts at:   4114    4114 
   Size:       5726
 
AleI CACnn'nnGTG

Cuts at:   1709    2282    1709 
   Size:        573    5153
 
AlwNI CAG_nnn'CTG

Cuts at:   2604    3031    4598    2604 
   Size:        427    1567    3732

  Fragments arranged by size: 

                3732    1567     427
 
ApaLI G'TGCA_C

Cuts at:   2081    4496    2081 
   Size:       2415    3311
 
ApoI r'AATT_y

Cuts at:    118     895    1460    2273    2357    2525    2730    5079    5335 
   Size:        777     565     813      84     168     205    2349     256

Cuts at:   5335     118 
   Size:        509

  Fragments arranged by size: 

                2349     813     777     565     509     256     205     168
                  84
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5726
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5726
 
AvaI C'yCGr_G

Cuts at:    175    2670    2746     175 
   Size:       2495      76    3155

  Fragments arranged by size: 

                3155    2495      76
 
BamHI G'GATC_C

Cuts at:   2751    2751 
   Size:       5726
 
BanI G'GyrC_C

Cuts at:   1118    2663    2742    3156    3404    3439    1118 
   Size:       1545      79     414     248      35    3405

  Fragments arranged by size: 

                3405    1545     414     248      79      35
 
BanII G_rGCy'C

Cuts at:   2251    2419    2740    3770    2251 
   Size:        168     321    1030    4207

  Fragments arranged by size: 

                4207    1030     321     168
 
BbeI G_GCGC'C

Cuts at:   3160    3408    3160 
   Size:        248    5478
 
BbsI GAAGACnn'nnnn_

Cuts at:    493    2145     493 
   Size:       1652    4074
 
BbvCI CC'TCA_GC

Cuts at:    113    2677     113 
   Size:       2564    3162
 
BciVI GTATCCnnnnn_n'

Cuts at:    881    3624    4385    5726     881 
   Size:       2743     761    1341     881

  Fragments arranged by size: 

                2743    1341     881     761
 
BclI T'GATC_A

Cuts at:   3246    3246 
   Size:       5726
 
BglII A'GATC_T

Cuts at:    288    1754    3241    5278     288 
   Size:       1466    1487    2037     736

  Fragments arranged by size: 

                2037    1487    1466     736
 
BlpI GC'TnA_GC

Cuts at:   2336    2838    2336 
   Size:        502    5224
 
Bme1580I G_kGCm'C

Cuts at:   2085    2589    3351    3444    4500    2085 
   Size:        504     762      93    1056    3311

  Fragments arranged by size: 

                3311    1056     762     504      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    174    1004    2459    2938    3019    3354    5498     174 
   Size:        830    1455     479      81     335    2144     402

  Fragments arranged by size: 

                2144    1455     830     479     402     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    696    4093     696 
   Size:       3397    2329
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    313     989    1322    2135    2387     313 
   Size:        676     333     813     252    3652

  Fragments arranged by size: 

                3652     813     676     333     252
 
Bpu10I CC'TnA_GC

Cuts at:    113    2677     113 
   Size:       2564    3162
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    115    1200    2902    3781    4273    4571     115 
   Size:       1085    1702     879     492     298    1270

  Fragments arranged by size: 

                1702    1270    1085     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    337    5430     337 
   Size:       5093     633
 
BsaAI yAC'GTr

Cuts at:   3079    3079 
   Size:       5726
 
BsaBI GATnn'nnATC

Cuts at:      6    1604    3240    5277       6 
   Size:       1598    1636    2037     455

  Fragments arranged by size: 

                2037    1636    1598     455
 
BsaHI Gr'CG_yC

Cuts at:   3157    3405    4107    5603    3157 
   Size:        248     702    1496    3280

  Fragments arranged by size: 

                3280    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    848    2435    2915    3436    4114    4388    4535    4946     848 
   Size:       1587     480     521     678     274     147     411    1628

  Fragments arranged by size: 

                1628    1587     678     521     480     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    250     280     759     789     250 
   Size:         30     479      30    5187

  Fragments arranged by size: 

                5187     479      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    347    1321     347 
   Size:        974    4752
 
BseYI C'CCAG_C

Cuts at:    782    3151    3909    4486    4982     782 
   Size:       2369     758     577     496    1526

  Fragments arranged by size: 

                2369    1526     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    3268    3314    4522     104 
   Size:       3164      46    1208    1308

  Fragments arranged by size: 

                3164    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   2039    2085    2251    2419    2740    3518    3708    4500    2039 
   Size:         46     166     168     321     778     190     792    3265

  Fragments arranged by size: 

                3265     792     778     321     190     168     166      46
            
 
BsmI GAATG_Cn'

Cuts at:    550     858    1084    1501    1585    2062    2230    2398     550 
   Size:        308     226     417      84     477     168     168    3878

  Fragments arranged by size: 

                3878     477     417     308     226     168     168      84
            
 
Bsp1286I G_dGCh'C

Cuts at:   2039    2085    2251    2419    2589    2740    3351    3444    3518 
   Size:         46     166     168     170     151     611      93      74

Cuts at:   3518    3708    3770    4500    2039 
   Size:        190      62     730    3265

  Fragments arranged by size: 

                3265     730     611     190     170     168     166     151
                  93      74      62      46
 
BspEI T'CCGG_A

Cuts at:   4946    4946 
   Size:       5726
 
BspHI T'CATG_A

Cuts at:    555    1014    4902     555 
   Size:        459    3888    1379

  Fragments arranged by size: 

                3888    1379     459
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2776    2990    3292    3673    2776 
   Size:        214     302     381    4829

  Fragments arranged by size: 

                4829     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    4018    4072    5556    5721      50 
   Size:       3968      54    1484     165      55

  Fragments arranged by size: 

                3968    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3638    3638 
   Size:       5726
 
BsrFI r'CCGG_y

Cuts at:   1430    1514    3724    3905    4114    1430 
   Size:         84    2210     181     209    3042

  Fragments arranged by size: 

                3042    2210     209     181      84
 
BsrGI T'GTAC_A

Cuts at:   2694    2694 
   Size:       5726
 
BssSI C'ACGA_G

Cuts at:   1705    1846    3997    4355    1705 
   Size:        141    2151     358    3076

  Fragments arranged by size: 

                3076    2151     358     141
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3103    3103 
   Size:       5726
 
BstBI TT'CG_AA

Cuts at:    377    4087     377 
   Size:       3710    2016
 
BstXI CCAn_nnnn'nTGG

Cuts at:    277     277 
   Size:       5726
 
BstYI r'GATC_y

Cuts at:      1     288    1100    1127    1364    1754    2751    3218    3241 
   Size:        287     812      27     237     390     997     467      23

Cuts at:   3241    3576    3822    4834    4920    4932    5278       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     997     812     467     449     390     346     335
                 287     246     237      86      27      23      12
 
BtgI C'CryG_G

Cuts at:    107     539     596     611     107 
   Size:        432      57      15    5222

  Fragments arranged by size: 

                5222     432      57      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3676    3857    4020    3676 
   Size:        181     163    5382

  Fragments arranged by size: 

                5382     181     163
 
BtsI GCAGTG_nn'

Cuts at:    288    1413    2001    2969     288 
   Size:       1125     588     968    3045

  Fragments arranged by size: 

                3045    1125     968     588
 
DraI TTT'AAA

Cuts at:   2046    2130    2725    2046 
   Size:         84     595    5047

  Fragments arranged by size: 

                5047     595      84
 
DrdI GACnn_nn'nnGTC

Cuts at:   3432    4290    3432 
   Size:        858    4868
 
EaeI y'GGCC_r

Cuts at:   1908    3311    3485    3876    3903    5248    1908 
   Size:       1403     174     391      27    1345    2386

  Fragments arranged by size: 

                2386    1403    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   3311    3311 
   Size:       5726
 
EarI CTCTTCn'nnn_

Cuts at:    238    1298    3749    3959     238 
   Size:       1060    2451     210    2005

  Fragments arranged by size: 

                2451    2005    1060     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2335    3934    4244    4390    5536    2335 
   Size:       1599     310     146    1146    2525

  Fragments arranged by size: 

                2525    1599    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2249    2417    2738    2249 
   Size:        168     321    5237

  Fragments arranged by size: 

                5237     321     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    313     744     989    1322    1326    1717    1999    2055    2135 
   Size:        431     245     333       4     391     282      56      80

Cuts at:   2135    2387    3551    3983    4730    4998     313 
   Size:        252    1164     432     747     268    1041

  Fragments arranged by size: 

                1164    1041     747     432     431     391     333     282
                 268     252     245      80      56       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:    302     302 
   Size:       5726
 
EcoO109I rG'GnC_Cy

Cuts at:    228    2865     228 
   Size:       2637    3089
 
EcoRI G'AATT_C

Cuts at:   1460    2273    2357    2525    2730    1460 
   Size:        813      84     168     205    4456

  Fragments arranged by size: 

                4456     813     205     168      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    441    3745     441 
   Size:       3304    2422
 
FspI TGC'GCA

Cuts at:   3507    3507 
   Size:       5726
 
HaeII r_GCGC'y

Cuts at:   3160    3408    4430    5409    3160 
   Size:        248    1022     979    3477

  Fragments arranged by size: 

                3477    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    397     429     759     791    1045    1077    1119    1151    1521 
   Size:         32     330      32     254      32      42      32     370

Cuts at:   1521    1553    1746    1778    2616    2648    3568    3598    3600 
   Size:         32     193      32     838      32     920      30       2

Cuts at:   3600    3630     397 
   Size:         30    2493

  Fragments arranged by size: 

                2493     920     838     370     330     254     193      42
                  32      32      32      32      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2641    2765    2641 
   Size:        124    5602
 
HindIII A'AGCT_T

Cuts at:    756    2570     756 
   Size:       1814    3912
 
KasI G'GCGC_C

Cuts at:   3156    3404    3156 
   Size:        248    5478
 
KpnI G_GTAC'C

Cuts at:   2746    2746 
   Size:       5726
 
MfeI C'AATT_G

Cuts at:    219     219 
   Size:       5726
 
MluI A'CGCG_T

Cuts at:   5159    5159 
   Size:       5726
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    194     713    1221    4397    4581     194 
   Size:        519     508    3176     184    1339

  Fragments arranged by size: 

                3176    1339     519     508     184
 
MscI TGG'CCA

Cuts at:   1910    3487    1910 
   Size:       1577    4149
 
MslI CAynn'nnrTG

Cuts at:    275     735     945    1445    1653    1709    2282    2690    3715 
   Size:        460     210     500     208      56     573     408    1025

Cuts at:   3715    3842     275 
   Size:        127    2159

  Fragments arranged by size: 

                2159    1025     573     500     460     408     210     208
                 127      56
 
MspA1I CmG'CkG

Cuts at:   2837    3151    3511    4524    4769    2837 
   Size:        314     360    1013     245    3794

  Fragments arranged by size: 

                3794    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1516    3907    1516 
   Size:       2391    3335
 
NarI GG'CG_CC

Cuts at:   3157    3405    3157 
   Size:        248    5478
 
NcoI C'CATG_G

Cuts at:    107     611     107 
   Size:        504    5222
 
NdeI CA'TA_TG

Cuts at:   1650    1650 
   Size:       5726
 
NgoMIV G'CCGG_C

Cuts at:   1514    3905    1514 
   Size:       2391    3335
 
NspI r_CATG'y

Cuts at:   2779    3279    4186    2779 
   Size:        500     907    4319

  Fragments arranged by size: 

                4319     907     500
 
PciI A'CATG_T

Cuts at:   4182    4182 
   Size:       5726
 
PmeI GTTT'AAAC

Cuts at:   2725    2725 
   Size:       5726
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4865    4897    4865 
   Size:         32    5694
 
PpuMI rG'GwC_Cy

Cuts at:    228     228 
   Size:       5726
 
PsiI TTA'TAA

Cuts at:   1316    1316 
   Size:       5726
 
PspXI vC'TCGA_Gb

Cuts at:   2670    2670 
   Size:       5726
 
PstI C_TGCA'G

Cuts at:   1828    2773    3458    1828 
   Size:        945     685    4096

  Fragments arranged by size: 

                4096     945     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5726
 
PvuII CAG'CTG

Cuts at:   3151    3511    3151 
   Size:        360    5366
 
RsrII CG'GwC_CG

Cuts at:   3921    3921 
   Size:       5726
 
SacI G_AGCT'C

Cuts at:   2251    2419    2740    2251 
   Size:        168     321    5237

  Fragments arranged by size: 

                5237     321     168
 
SalI G'TCGA_C

Cuts at:   2763    2763 
   Size:       5726
 
SapI GCTCTTCn'nnn_

Cuts at:   1298    3749    3959    1298 
   Size:       2451     210    3065

  Fragments arranged by size: 

                3065    2451     210
 
SbfI CC_TGCA'GG

Cuts at:   2773    2773 
   Size:       5726
 
ScaI AGT'ACT

Cuts at:   1917    5243    1917 
   Size:       3326    2400
 
SexAI A'CCwGG_T

Cuts at:    466     466 
   Size:       5726
 
SfcI C'TryA_G

Cuts at:     33    1473    1566    1824    2463    2769    3454    4447    4638 
   Size:       1440      93     258     639     306     685     993     191

Cuts at:   4638      33 
   Size:       1121

  Fragments arranged by size: 

                1440    1121     993     685     639     306     258     191
                  93
 
SfoI GGC'GCC

Cuts at:   3158    3406    3158 
   Size:        248    5478
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5726
 
SmaI CCC'GGG

Cuts at:   2748    2748 
   Size:       5726
 
SmlI C'TyrA_G

Cuts at:    130    1179    2670    2881    3796    4288    4550     130 
   Size:       1049    1491     211     915     492     262    1306

  Fragments arranged by size: 

                1491    1306    1049     915     492     262     211
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5726
 
SphI G_CATG'C

Cuts at:   2779    3279    2779 
   Size:        500    5226
 
StuI AGG'CCT

Cuts at:   1423    2320    1423 
   Size:        897    4829
 
StyI C'CwwG_G

Cuts at:    107     155     416     611    2860    3214     107 
   Size:         48     261     195    2249     354    2619

  Fragments arranged by size: 

                2619    2249     354     261     195      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    287    1019    1023    2173    2425    2632    3309    3442    3901 
   Size:        732       4    1150     252     207     677     133     459

Cuts at:   3901    4110     287 
   Size:        209    1903

  Fragments arranged by size: 

                1903    1150     732     677     459     252     209     207
                 133       4
 
TatI w'GTAC_w

Cuts at:    937    1915    2694    5241     937 
   Size:        978     779    2547    1422

  Fragments arranged by size: 

                2547    1422     978     779
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1727    1759    5243    5275    1727 
   Size:         32    3484      32    2178

  Fragments arranged by size: 

                3484    2178      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    575    2149    3523     575 
   Size:       1574    1374    2778

  Fragments arranged by size: 

                2778    1574    1374
 
XbaI T'CTAG_A

Cuts at:   1948    2757    1948 
   Size:        809    4917
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3221    3221 
   Size:       5726
 
XhoI C'TCGA_G

Cuts at:   2670    2670 
   Size:       5726
 
XmaI C'CCGG_G

Cuts at:   2746    2746 
   Size:       5726
 
XmnI GAAnn'nnTTC

Cuts at:    500     757    1339    1423    2236    2320    2404     500 
   Size:        257     582      84     813      84      84    3822

  Fragments arranged by size: 

                3822     813     582     257      84      84      84
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI     PciI
     PmeI     PpiI    PpuMI     PsiI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI     BmtI
     BsgI    BsiWI    BsmBI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoRV     FseI    FspAI     HpaI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PshAI
   PspOMI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     ZraI
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