Restriction map for pF1KA1141
 (Linear) (Six-base) MAPSORT of: KIAA1141.seq  Check: 5115  from: 1  to: 2613



KIAA1141.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

AclI AA'CG_TT

Cuts at:      0    2128    2535    2613 
   Size:       2128     407      78

  Fragments arranged by size: 

                2128     407      78
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     636    1218    1609    1891    1947    2613 
   Size:        636     582     391     282      56     666

  Fragments arranged by size: 

                 666     636     582     391     282      56
 
AflIII A'CryG_T

Cuts at:      0      86    2613 
   Size:         86    2527
 
AleI CACnn'nnGTG

Cuts at:      0    1601    2174    2613 
   Size:       1601     573     439

  Fragments arranged by size: 

                1601     573     439
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2496    2613 
   Size:       2496     117
 
ApaLI G'TGCA_C

Cuts at:      0    1973    2613 
   Size:       1973     640
 
ApoI r'AATT_y

Cuts at:      0      10     787    1352    2165    2249    2417    2613 
   Size:         10     777     565     813      84     168     196

  Fragments arranged by size: 

                 813     777     565     196     168      84      10
 
AvaI C'yCGr_G

Cuts at:      0      67    2562    2613 
   Size:         67    2495      51

  Fragments arranged by size: 

                2495      67      51
 
BanI G'GyrC_C

Cuts at:      0    1010    2555    2613 
   Size:       1010    1545      58

  Fragments arranged by size: 

                1545    1010      58
 
BanII G_rGCy'C

Cuts at:      0    2143    2311    2613 
   Size:       2143     168     302

  Fragments arranged by size: 

                2143     302     168
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     385    2037    2613 
   Size:        385    1652     576

  Fragments arranged by size: 

                1652     576     385
 
BbvCI CC'TCA_GC

Cuts at:      0       5    2569    2613 
   Size:          5    2564      44

  Fragments arranged by size: 

                2564      44       5
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     773    2613 
   Size:        773    1840
 
BglII A'GATC_T

Cuts at:      0     180    1646    2613 
   Size:        180    1466     967

  Fragments arranged by size: 

                1466     967     180
 
BlpI GC'TnA_GC

Cuts at:      0    2228    2613 
   Size:       2228     385
 
Bme1580I G_kGCm'C

Cuts at:      0    1977    2481    2613 
   Size:       1977     504     132

  Fragments arranged by size: 

                1977     504     132
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      66     896    2351    2613 
   Size:         66     830    1455     262

  Fragments arranged by size: 

                1455     830     262      66
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     588    2613 
   Size:        588    2025
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     881    1214    2027    2279    2613 
   Size:        205     676     333     813     252     334

  Fragments arranged by size: 

                 813     676     334     333     252     205
 
Bpu10I CC'TnA_GC

Cuts at:      0       5    2569    2613 
   Size:          5    2564      44

  Fragments arranged by size: 

                2564      44       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7    1092    2613 
   Size:          7    1085    1521

  Fragments arranged by size: 

                1521    1085       7
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     229    2613 
   Size:        229    2384
 
BsaBI GATnn'nnATC

Cuts at:      0    1496    2613 
   Size:       1496    1117
 
BsaWI w'CCGG_w

Cuts at:      0     740    2327    2613 
   Size:        740    1587     286

  Fragments arranged by size: 

                1587     740     286
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     142     172     651     681    2613 
   Size:        142      30     479      30    1932

  Fragments arranged by size: 

                1932     479     142      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     239    1213    2613 
   Size:        239     974    1400

  Fragments arranged by size: 

                1400     974     239
 
BseYI C'CCAG_C

Cuts at:      0     674    2613 
   Size:        674    1939
 
BsiHKAI G_wGCw'C

Cuts at:      0    1931    1977    2143    2311    2613 
   Size:       1931      46     166     168     302

  Fragments arranged by size: 

                1931     302     168     166      46
 
BsmI GAATG_Cn'

Cuts at:      0     442     750     976    1393    1477    1954    2122    2290 
   Size:        442     308     226     417      84     477     168     168

Cuts at:   2290    2613 
   Size:        323

  Fragments arranged by size: 

                 477     442     417     323     308     226     168     168
                  84
 
Bsp1286I G_dGCh'C

Cuts at:      0    1931    1977    2143    2311    2481    2613 
   Size:       1931      46     166     168     170     132

  Fragments arranged by size: 

                1931     170     168     166     132      46
 
BspHI T'CATG_A

Cuts at:      0     447     906    2613 
   Size:        447     459    1707

  Fragments arranged by size: 

                1707     459     447
 
BsrFI r'CCGG_y

Cuts at:      0    1322    1406    2613 
   Size:       1322      84    1207

  Fragments arranged by size: 

                1322    1207      84
 
BsrGI T'GTAC_A

Cuts at:      0    2586    2613 
   Size:       2586      27
 
BssSI C'ACGA_G

Cuts at:      0    1597    1738    2613 
   Size:       1597     141     875

  Fragments arranged by size: 

                1597     875     141
 
BstBI TT'CG_AA

Cuts at:      0     269    2613 
   Size:        269    2344
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     169    2613 
   Size:        169    2444
 
BstYI r'GATC_y

Cuts at:      0     180     992    1019    1256    1646    2613 
   Size:        180     812      27     237     390     967

  Fragments arranged by size: 

                 967     812     390     237     180      27
 
BtgI C'CryG_G

Cuts at:      0     431     488     503    2613 
   Size:        431      57      15    2110

  Fragments arranged by size: 

                2110     431      57      15
 
BtsI GCAGTG_nn'

Cuts at:      0     180    1305    1893    2613 
   Size:        180    1125     588     720

  Fragments arranged by size: 

                1125     720     588     180
 
DraI TTT'AAA

Cuts at:      0    1938    2022    2613 
   Size:       1938      84     591

  Fragments arranged by size: 

                1938     591      84
 
EaeI y'GGCC_r

Cuts at:      0    1800    2613 
   Size:       1800     813
 
EarI CTCTTCn'nnn_

Cuts at:      0     130    1190    2613 
   Size:        130    1060    1423

  Fragments arranged by size: 

                1423    1060     130
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2227    2613 
   Size:       2227     386
 
EcoICRI GAG'CTC

Cuts at:      0    2141    2309    2613 
   Size:       2141     168     304

  Fragments arranged by size: 

                2141     304     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     636     881    1214    1218    1609    1891    1947 
   Size:        205     431     245     333       4     391     282      56

Cuts at:   1947    2027    2279    2613 
   Size:         80     252     334

  Fragments arranged by size: 

                 431     391     334     333     282     252     245     205
                  80      56       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     194    2613 
   Size:        194    2419
 
EcoO109I rG'GnC_Cy

Cuts at:      0     120    2613 
   Size:        120    2493
 
EcoRI G'AATT_C

Cuts at:      0    1352    2165    2249    2417    2613 
   Size:       1352     813      84     168     196

  Fragments arranged by size: 

                1352     813     196     168      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     333    2613 
   Size:        333    2280
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     289     321     651     683     937     969    1011    1043 
   Size:        289      32     330      32     254      32      42      32

Cuts at:   1043    1413    1445    1638    1670    2508    2540    2613 
   Size:        370      32     193      32     838      32      73

  Fragments arranged by size: 

                 838     370     330     289     254     193      73      42
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2533    2613 
   Size:       2533      80
 
HindIII A'AGCT_T

Cuts at:      0     648    2462    2613 
   Size:        648    1814     151

  Fragments arranged by size: 

                1814     648     151
 
MfeI C'AATT_G

Cuts at:      0     111    2613 
   Size:        111    2502
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     605    1113    2613 
   Size:         86     519     508    1500

  Fragments arranged by size: 

                1500     519     508      86
 
MscI TGG'CCA

Cuts at:      0    1802    2613 
   Size:       1802     811
 
MslI CAynn'nnrTG

Cuts at:      0     167     627     837    1337    1545    1601    2174    2582 
   Size:        167     460     210     500     208      56     573     408

Cuts at:   2582    2613 
   Size:         31

  Fragments arranged by size: 

                 573     500     460     408     210     208     167      56
                  31
 
NaeI GCC'GGC

Cuts at:      0    1408    2613 
   Size:       1408    1205
 
NcoI C'CATG_G

Cuts at:      0     503    2613 
   Size:        503    2110
 
NdeI CA'TA_TG

Cuts at:      0    1542    2613 
   Size:       1542    1071
 
NgoMIV G'CCGG_C

Cuts at:      0    1406    2613 
   Size:       1406    1207
 
PpuMI rG'GwC_Cy

Cuts at:      0     120    2613 
   Size:        120    2493
 
PsiI TTA'TAA

Cuts at:      0    1208    2613 
   Size:       1208    1405
 
PspXI vC'TCGA_Gb

Cuts at:      0    2562    2613 
   Size:       2562      51
 
PstI C_TGCA'G

Cuts at:      0    1720    2613 
   Size:       1720     893
 
SacI G_AGCT'C

Cuts at:      0    2143    2311    2613 
   Size:       2143     168     302

  Fragments arranged by size: 

                2143     302     168
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1190    2613 
   Size:       1190    1423
 
ScaI AGT'ACT

Cuts at:      0    1809    2613 
   Size:       1809     804
 
SexAI A'CCwGG_T

Cuts at:      0     358    2613 
   Size:        358    2255
 
SfcI C'TryA_G

Cuts at:      0    1365    1458    1716    2355    2613 
   Size:       1365      93     258     639     258

  Fragments arranged by size: 

                1365     639     258     258      93
 
SmlI C'TyrA_G

Cuts at:      0      22    1071    2562    2613 
   Size:         22    1049    1491      51

  Fragments arranged by size: 

                1491    1049      51      22
 
StuI AGG'CCT

Cuts at:      0    1315    2212    2613 
   Size:       1315     897     401

  Fragments arranged by size: 

                1315     897     401
 
StyI C'CwwG_G

Cuts at:      0      47     308     503    2613 
   Size:         47     261     195    2110

  Fragments arranged by size: 

                2110     261     195      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     179     911     915    2065    2317    2524    2613 
   Size:        179     732       4    1150     252     207      89

  Fragments arranged by size: 

                1150     732     252     207     179      89       4
 
TatI w'GTAC_w

Cuts at:      0     829    1807    2586    2613 
   Size:        829     978     779      27

  Fragments arranged by size: 

                 978     829     779      27
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1619    1651    2613 
   Size:       1619      32     962

  Fragments arranged by size: 

                1619     962      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     467    2041    2613 
   Size:        467    1574     572

  Fragments arranged by size: 

                1574     572     467
 
XbaI T'CTAG_A

Cuts at:      0    1840    2613 
   Size:       1840     773
 
XhoI C'TCGA_G

Cuts at:      0    2562    2613 
   Size:       2562      51
 
XmnI GAAnn'nnTTC

Cuts at:      0     392     649    1231    1315    2128    2212    2296    2613 
   Size:        392     257     582      84     813      84      84     317

  Fragments arranged by size: 

                 813     582     392     317     257      84      84      84
            
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AleI    AlwNI    ApaLI     ApoI     AvaI
     BanI    BanII     BbsI    BbvCI    BciVI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrFI
    BsrGI    BssSI    BstBI    BstXI    BstYI     BtgI     BtsI     DraI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI    Hin4I   HincII  HindIII     MfeI     MmeI     MscI     MslI
     NaeI     NcoI     NdeI   NgoMIV    PpuMI     PsiI    PspXI     PstI
     SacI     SapI     ScaI    SexAI     SfcI     SmlI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BcgI     BclI    BfrBI     BglI    BmgBI     BmtI
    BsaAI    BsaHI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI   BssHII   BstAPI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI   DraIII     DrdI     EagI    EcoRV     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MluI   MspA1I     NarI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XcmI     XmaI     ZraI
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