Restriction map for pF1KA1124
##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
AocI        Aor51HI     AscI        AseI        AsnI        
Asp718I     AvrII       BamHI       BbeI        BciVI       
BclI        BfrI        BglII       BlnI        BsiEI       
BsiWI       BsmBI       BsmI        BspDI       Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoT22I     FbaI        
FseI        HincII      HindII      HpaI        KasI        
KpnI        KspI        MfeI        MluI        MunI        
NarI        NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SwaI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AatII        4453  GACGTC               GACGT^C
AhdI          787  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         787  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          610  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BglI         2644  GCCCTGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BspHI         878  TCATGA               T^CATGA
BspLU11I     2615  ACATGT               A^CATGT
BstXI        4871  CCACCAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I      787  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        4746  GATATC               GAT^ATC
NcoI         4472  CCATGG               C^CATGG
NdeI         3350  CATATG               CA^TATG
PflFI         610  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        3632  GACTCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          878  TCATGA               T^CATGA
SexAI        4220  ACCTGGT              A^CC[AT]GGT
SmaI         2492  CCCGGG               CCC^GGG
SspI         1143  AATATT               AAT^ATT
Tth111I       610  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3624  TCTAGA               T^CTAGA
XmaCI        2492  CCCGGG               C^CCGGG
XmaI         2492  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccIII        516  TCCGGA               T^CCGGA
             3966  TCCGGA              
AflIII       2615  ACATGT               A^C[GA][CT]GT
             3191  ACGTGT              
Asp700I      2458  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4080  GAAGCCATTC          
AviII        4466  TGCGCA               TGC^GCA
             4643  TGCGCA              
BbrPI        3190  CACGTG               CAC^GTG
             4771  CACGTG              
BcgI         2481  CGAGAAAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              207  GCAGCTTCATCG        
BsaBI        1693  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3873  GATCGTAATC          
BsaWI         516  TCCGGA               [AT]^CCGG[AT]
             3966  TCCGGA              
BseAI         516  TCCGGA               T^CCGGA
             3966  TCCGGA              
Bsp1407I      110  TGTACA               T^GTACA
             4433  TGTACA              
BspEI         516  TCCGGA               T^CCGGA
             3966  TCCGGA              
BsrDI        3423  CATTGC               GCAATG(2/0)
              307  GCAATG              
BsrGI         110  TGTACA               T^GTACA
             4433  TGTACA              
BssHII       3345  GCGCGC               G^CGCGC
             4367  GCGCGC              
BssSI         856  CTCGTG               CACGAG(-5/-1)
             4917  CTCGTG              
BstEII       2497  GGTTACC              G^GT[ACGT]ACC
             3798  GGTCACC             
BstPI        2497  GGTTACC              G^GT[ACGT]ACC
             3798  GGTCACC             
DrdI           94  GACGTGCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1398  GACCGTGCAGTC        
EcoO65I      2497  GGTTACC              G^GT[ACGT]ACC
             3798  GGTCACC             
FspI         4466  TGCGCA               TGC^GCA
             4643  TGCGCA              
HaeII          67  AGCGCC               [GA]GCGC^[CT]
             1545  GGCGCT              
MamI         1693  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3873  GATCGTAATC          
MroI          516  TCCGGA               T^CCGGA
             3966  TCCGGA              
MslI         3321  CACGGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4800  CATGCAGGTG          
NaeI          977  GCCGGC               GCC^GGC
             5118  GCCGGC              
NgoMIV        977  GCCGGC               G^CCGGC
             5118  GCCGGC              
PmaCI        3190  CACGTG               CAC^GTG
             4771  CACGTG              
PmlI         3190  CACGTG               CAC^GTG
             4771  CACGTG              
SbfI          741  CCTGCAGG             CCTGCA^GG
             1719  CCTGCAGG            
SphI         1014  GCATGC               GCATG^C
             4799  GCATGC              
Sse8387I      741  CCTGCAGG             CCTGCA^GG
             1719  CCTGCAGG            
SspBI         110  TGTACA               T^GTACA
             4433  TGTACA              
XmnI         2458  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4080  GAAGCCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  130 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       ApoI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PaeR7I      PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com