Restriction map for pF1KA0982
 (Circular) (Six-base) MAPSORT of: pF1KA0982.seq  Check: 1929  from: 1  to: 4595



pF1KA0982  4595 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AatII G_ACGT'C

Cuts at:   1045    1045 
   Size:       4595
 
AccI GT'mk_AC

Cuts at:   1633    1633 
   Size:       4595
 
Acc65I G'GTAC_C

Cuts at:   1611    1611 
   Size:       4595
 
AclI AA'CG_TT

Cuts at:    372    4435     372 
   Size:       4063     532
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    404     493    2420    2852    3599    3867     404 
   Size:         89    1927     432     747     268    1132

  Fragments arranged by size: 

                1927    1132     747     432     268      89
 
AflIII A'CryG_T

Cuts at:    230    1947    3051    4028     230 
   Size:       1717    1104     977     797

  Fragments arranged by size: 

                1717    1104     977     797
 
AgeI A'CCGG_T

Cuts at:   2983    2983 
   Size:       4595
 
AlwNI CAG_nnn'CTG

Cuts at:    471    1900    3467     471 
   Size:       1429    1567    1599

  Fragments arranged by size: 

                1599    1567    1429
 
ApaLI G'TGCA_C

Cuts at:   3365    3365 
   Size:       4595
 
ApoI r'AATT_y

Cuts at:    310     714     724    1599    3948    4204     310 
   Size:        404      10     875    2349     256     701

  Fragments arranged by size: 

                2349     875     701     404     256      10
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4595
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4595
 
AvaI C'yCGr_G

Cuts at:    622    1615     622 
   Size:        993    3602
 
BamHI G'GATC_C

Cuts at:   1620    1620 
   Size:       4595
 
BanI G'GyrC_C

Cuts at:   1611    2025    2273    2308    1611 
   Size:        414     248      35    3898

  Fragments arranged by size: 

                3898     414     248      35
 
BanII G_rGCy'C

Cuts at:    709     878    1052    1609    2639     709 
   Size:        169     174     557    1030    2665

  Fragments arranged by size: 

                2665    1030     557     174     169
 
BbeI G_GCGC'C

Cuts at:   2029    2277    2029 
   Size:        248    4347
 
BbsI GAAGACnn'nnnn_

Cuts at:    481    1505     481 
   Size:       1024    3571
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     113     124     147      90 
   Size:         23      11      23    4538

  Fragments arranged by size: 

                4538      23      23      11
 
BciVI GTATCCnnnnn_n'

Cuts at:   2493    3254    4595    2493 
   Size:        761    1341    2493

  Fragments arranged by size: 

                2493    1341     761
 
BclI T'GATC_A

Cuts at:   2115    2115 
   Size:       4595
 
BglI GCCn_nnn'nGGC

Cuts at:    704     950    1460     704 
   Size:        246     510    3839

  Fragments arranged by size: 

                3839     510     246
 
BglII A'GATC_T

Cuts at:   2110    4147    2110 
   Size:       2037    2558
 
BlpI GC'TnA_GC

Cuts at:   1707    1707 
   Size:       4595
 
Bme1580I G_kGCm'C

Cuts at:   1073    2220    2313    3369    1073 
   Size:       1147      93    1056    2299

  Fragments arranged by size: 

                2299    1147    1056      93
 
BmgBI CAC'GTC

Cuts at:   1035    1035 
   Size:       4595
 
BmrI ACTGGGnnnn_n'

Cuts at:   1807    1888    2223    4367    1807 
   Size:         81     335    2144    2035

  Fragments arranged by size: 

                2144    2035     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2962    2962 
   Size:       4595
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    495     782     804     495 
   Size:        287      22    4286

  Fragments arranged by size: 

                4286     287      22
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    922    1771    2650    3142    3440     922 
   Size:        849     879     492     298    2077

  Fragments arranged by size: 

                2077     879     849     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4299    4299 
   Size:       4595
 
BsaAI yAC'GTr

Cuts at:    346     702    1948     346 
   Size:        356    1246    2993

  Fragments arranged by size: 

                2993    1246     356
 
BsaBI GATnn'nnATC

Cuts at:      6    2109    4146       6 
   Size:       2103    2037     455

  Fragments arranged by size: 

                2103    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1042    2026    2274    2976    4472    1042 
   Size:        984     248     702    1496    1165

  Fragments arranged by size: 

                1496    1165     984     702     248
 
BsaWI w'CCGG_w

Cuts at:   1013    1784    2305    2983    3257    3404    3815    1013 
   Size:        771     521     678     274     147     411    1793

  Fragments arranged by size: 

                1793     771     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1232    1262    1232 
   Size:         30    4565
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1119    1119 
   Size:       4595
 
BseYI C'CCAG_C

Cuts at:    812    1109    2020    2778    3355    3851     812 
   Size:        297     911     758     577     496    1556

  Fragments arranged by size: 

                1556     911     758     577     496     297
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    817    1080     817 
   Size:        263    4332
 
BsiEI CG_ry'CG

Cuts at:    104    2137    2183    3391     104 
   Size:       2033      46    1208    1308

  Fragments arranged by size: 

                2033    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    623     862    1052    1609    2387    2577    3369     623 
   Size:        239     190     557     778     190     792    1849

  Fragments arranged by size: 

                1849     792     778     557     239     190     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1033    1033 
   Size:       4595
 
Bsp1286I G_dGCh'C

Cuts at:    623     709     862     878    1052    1073    1609    2220    2313 
   Size:         86     153      16     174      21     536     611      93

Cuts at:   2313    2387    2577    2639    3369     623 
   Size:         74     190      62     730    1849

  Fragments arranged by size: 

                1849     730     611     536     190     174     153      93
                  86      74      62      21      16
 
BspEI T'CCGG_A

Cuts at:   3815    3815 
   Size:       4595
 
BspHI T'CATG_A

Cuts at:   3771    3771 
   Size:       4595
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1645    1859    2161    2542    1645 
   Size:        214     302     381    3698

  Fragments arranged by size: 

                3698     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2887    2941    4425    4590      50 
   Size:       2837      54    1484     165      55

  Fragments arranged by size: 

                2837    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1310    1573    2507    1310 
   Size:        263     934    3398

  Fragments arranged by size: 

                3398     934     263
 
BsrFI r'CCGG_y

Cuts at:   1538    2593    2774    2983    1538 
   Size:       1055     181     209    3150

  Fragments arranged by size: 

                3150    1055     209     181
 
BssSI C'ACGA_G

Cuts at:    550    1117    2866    3224     550 
   Size:        567    1749     358    1921

  Fragments arranged by size: 

                1921    1749     567     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1972    1972 
   Size:       4595
 
BstBI TT'CG_AA

Cuts at:   2956    2956 
   Size:       4595
 
BstEII G'GTnAC_C

Cuts at:   1122    1122 
   Size:       4595
 
BstYI r'GATC_y

Cuts at:      1    1303    1620    2087    2110    2445    2691    3703    3789 
   Size:       1302     317     467      23     335     246    1012      86

Cuts at:   3789    3801    4147       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1302    1012     467     449     346     335     317     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:   1235    1235 
   Size:       4595
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    945    1277    2545    2726    2889     945 
   Size:        332    1268     181     163    2651

  Fragments arranged by size: 

                2651    1268     332     181     163
 
BtsI GCAGTG_nn'

Cuts at:    752    1089    1838     752 
   Size:        337     749    3509

  Fragments arranged by size: 

                3509     749     337
 
DraI TTT'AAA

Cuts at:   1594    1594 
   Size:       4595
 
DraIII CAC_nnn'GTG

Cuts at:   1185    1509    1185 
   Size:        324    4271
 
DrdI GACnn_nn'nnGTC

Cuts at:   2301    3159    2301 
   Size:        858    3737
 
EaeI y'GGCC_r

Cuts at:    567     599    1362    2180    2354    2745    2772    4117     567 
   Size:         32     763     818     174     391      27    1345    1045

  Fragments arranged by size: 

                1345    1045     818     763     391     174      32      27
            
 
EagI C'GGCC_G

Cuts at:   2180    2180 
   Size:       4595
 
EarI CTCTTCn'nnn_

Cuts at:   2618    2828    2618 
   Size:        210    4385
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1138    2803    3113    3259    4405    1138 
   Size:       1665     310     146    1146    1328

  Fragments arranged by size: 

                1665    1328    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1050    1607    1050 
   Size:        557    4038
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    404     493     495     782     804    2420    2852    3599    3867 
   Size:         89       2     287      22    1616     432     747     268

Cuts at:   3867     404 
   Size:       1132

  Fragments arranged by size: 

                1616    1132     747     432     287     268      89      22
                   2
 
EcoO109I rG'GnC_Cy

Cuts at:   1167    1734    1167 
   Size:        567    4028
 
EcoRI G'AATT_C

Cuts at:   1599    1599 
   Size:       4595
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2614    2614 
   Size:       4595
 
FspI TGC'GCA

Cuts at:   2376    2376 
   Size:       4595
 
HaeII r_GCGC'y

Cuts at:   2029    2277    3299    4278    2029 
   Size:        248    1022     979    2346

  Fragments arranged by size: 

                2346    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2437    2467    2469    2499    2437 
   Size:         30       2      30    4533

  Fragments arranged by size: 

                4533      30      30       2
 
HincII GTy'rAC

Cuts at:   1085    1634    1085 
   Size:        549    4046
 
KasI G'GCGC_C

Cuts at:   2025    2273    2025 
   Size:        248    4347
 
KpnI G_GTAC'C

Cuts at:   1615    1615 
   Size:       4595
 
MluI A'CGCG_T

Cuts at:   4028    4028 
   Size:       4595
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    992    1534    3266    3450     992 
   Size:        542    1732     184    2137

  Fragments arranged by size: 

                2137    1732     542     184
 
MscI TGG'CCA

Cuts at:    601    2356     601 
   Size:       1755    2840
 
MslI CAynn'nnrTG

Cuts at:   2584    2711    2584 
   Size:        127    4468
 
MspA1I CmG'CkG

Cuts at:    801    1113    1706    2020    2380    3393    3638     801 
   Size:        312     593     314     360    1013     245    1758

  Fragments arranged by size: 

                1758    1013     593     360     314     312     245
 
NaeI GCC'GGC

Cuts at:   2776    2776 
   Size:       4595
 
NarI GG'CG_CC

Cuts at:   2026    2274    2026 
   Size:        248    4347
 
NgoMIV G'CCGG_C

Cuts at:   2774    2774 
   Size:       4595
 
NspI r_CATG'y

Cuts at:    234    1648    2148    3055     234 
   Size:       1414     500     907    1774

  Fragments arranged by size: 

                1774    1414     907     500
 
PciI A'CATG_T

Cuts at:    230    3051     230 
   Size:       2821    1774
 
PflMI CCAn_nnn'nTGG

Cuts at:    954    1553     954 
   Size:        599    3996
 
PmeI GTTT'AAAC

Cuts at:   1594    1594 
   Size:       4595
 
PmlI CAC'GTG

Cuts at:    702     702 
   Size:       4595
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3734    3766    3734 
   Size:         32    4563
 
PpuMI rG'GwC_Cy

Cuts at:   1167    1167 
   Size:       4595
 
PstI C_TGCA'G

Cuts at:   1642    2327    1642 
   Size:        685    3910
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4595
 
PvuII CAG'CTG

Cuts at:    801    2020    2380     801 
   Size:       1219     360    3016

  Fragments arranged by size: 

                3016    1219     360
 
RsrII CG'GwC_CG

Cuts at:   2790    2790 
   Size:       4595
 
SacI G_AGCT'C

Cuts at:   1052    1609    1052 
   Size:        557    4038
 
SalI G'TCGA_C

Cuts at:   1632    1632 
   Size:       4595
 
SanDI GG'GwC_CC

Cuts at:   1167    1167 
   Size:       4595
 
SapI GCTCTTCn'nnn_

Cuts at:   2618    2828    2618 
   Size:        210    4385
 
SbfI CC_TGCA'GG

Cuts at:   1642    1642 
   Size:       4595
 
ScaI AGT'ACT

Cuts at:   4112    4112 
   Size:       4595
 
SfcI C'TryA_G

Cuts at:     33    1638    2323    3316    3507      33 
   Size:       1605     685     993     191    1121

  Fragments arranged by size: 

                1605    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   2027    2275    2027 
   Size:        248    4347
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4595
 
SmaI CCC'GGG

Cuts at:   1617    1617 
   Size:       4595
 
SmlI C'TyrA_G

Cuts at:    937    1750    2665    3157    3419     937 
   Size:        813     915     492     262    2113

  Fragments arranged by size: 

                2113     915     813     492     262
 
SnaBI TAC'GTA

Cuts at:    346     346 
   Size:       4595
 
SpeI A'CTAG_T

Cuts at:     67     829      67 
   Size:        762    3833
 
SphI G_CATG'C

Cuts at:   1648    2148    1648 
   Size:        500    4095
 
StyI C'CwwG_G

Cuts at:   1729    2083    1729 
   Size:        354    4241
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1123    2178    2311    2770    2979    1123 
   Size:       1055     133     459     209    2739

  Fragments arranged by size: 

                2739    1055     459     209     133
 
TatI w'GTAC_w

Cuts at:    274     560    4110     274 
   Size:        286    3550     759

  Fragments arranged by size: 

                3550     759     286
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1232    1264    4112    4144    1232 
   Size:         32    2848      32    1683

  Fragments arranged by size: 

                2848    1683      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2392    2392 
   Size:       4595
 
XbaI T'CTAG_A

Cuts at:   1626    1626 
   Size:       4595
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2090    2090 
   Size:       4595
 
XmaI C'CCGG_G

Cuts at:   1615    1615 
   Size:       4595
 
XmnI GAAnn'nnTTC

Cuts at:   1192    1192 
   Size:       4595
 
ZraI GAC'GTC

Cuts at:   1043    1043 
   Size:       4595
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI     BmtI   Bpu10I    BsiWI
     BsmI    BsrGI   BssHII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SexAI     SfiI
    SgrAI     SrfI     SspI     StuI     SwaI     XhoI
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