Restriction map for pF1KA0982
 (Linear) (Six-base) MAPSORT of: KIAA0982.seq  Check: 3992  from: 1  to: 1482



KIAA0982.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AatII G_ACGT'C

Cuts at:      0     937    1482 
   Size:        937     545
 
AclI AA'CG_TT

Cuts at:      0     264    1482 
   Size:        264    1218
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     385    1482 
   Size:        296      89    1097

  Fragments arranged by size: 

                1097     296      89
 
AflIII A'CryG_T

Cuts at:      0     122    1482 
   Size:        122    1360
 
AlwNI CAG_nnn'CTG

Cuts at:      0     363    1482 
   Size:        363    1119
 
ApoI r'AATT_y

Cuts at:      0     202     606     616    1482 
   Size:        202     404      10     866

  Fragments arranged by size: 

                 866     404     202      10
 
AvaI C'yCGr_G

Cuts at:      0     514    1482 
   Size:        514     968
 
BanII G_rGCy'C

Cuts at:      0     601     770     944    1482 
   Size:        601     169     174     538

  Fragments arranged by size: 

                 601     538     174     169
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     373    1397    1482 
   Size:        373    1024      85

  Fragments arranged by size: 

                1024     373      85
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       5      39    1482 
   Size:          5      34    1443

  Fragments arranged by size: 

                1443      34       5
 
BglI GCCn_nnn'nGGC

Cuts at:      0     596     842    1352    1482 
   Size:        596     246     510     130

  Fragments arranged by size: 

                 596     510     246     130
 
Bme1580I G_kGCm'C

Cuts at:      0     965    1482 
   Size:        965     517
 
BmgBI CAC'GTC

Cuts at:      0     927    1482 
   Size:        927     555
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     387     674     696    1482 
   Size:        387     287      22     786

  Fragments arranged by size: 

                 786     387     287      22
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     814    1482 
   Size:        814     668
 
BsaAI yAC'GTr

Cuts at:      0     238     594    1482 
   Size:        238     356     888

  Fragments arranged by size: 

                 888     356     238
 
BsaHI Gr'CG_yC

Cuts at:      0     934    1482 
   Size:        934     548
 
BsaWI w'CCGG_w

Cuts at:      0     905    1482 
   Size:        905     577
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1124    1154    1482 
   Size:       1124      30     328

  Fragments arranged by size: 

                1124     328      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1011    1482 
   Size:       1011     471
 
BseYI C'CCAG_C

Cuts at:      0     704    1001    1482 
   Size:        704     297     481

  Fragments arranged by size: 

                 704     481     297
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     709     972    1482 
   Size:        709     263     510

  Fragments arranged by size: 

                 709     510     263
 
BsiHKAI G_wGCw'C

Cuts at:      0     515     754     944    1482 
   Size:        515     239     190     538

  Fragments arranged by size: 

                 538     515     239     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     925    1482 
   Size:        925     557
 
Bsp1286I G_dGCh'C

Cuts at:      0     515     601     754     770     944     965    1482 
   Size:        515      86     153      16     174      21     517

  Fragments arranged by size: 

                 517     515     174     153      86      21      16
 
BsrDI GCAATG_nn'

Cuts at:      0    1202    1465    1482 
   Size:       1202     263      17

  Fragments arranged by size: 

                1202     263      17
 
BsrFI r'CCGG_y

Cuts at:      0    1430    1482 
   Size:       1430      52
 
BssSI C'ACGA_G

Cuts at:      0     442    1009    1482 
   Size:        442     567     473

  Fragments arranged by size: 

                 567     473     442
 
BstEII G'GTnAC_C

Cuts at:      0    1014    1482 
   Size:       1014     468
 
BstYI r'GATC_y

Cuts at:      0    1195    1482 
   Size:       1195     287
 
BtgI C'CryG_G

Cuts at:      0    1127    1482 
   Size:       1127     355
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     837    1169    1482 
   Size:        837     332     313

  Fragments arranged by size: 

                 837     332     313
 
BtsI GCAGTG_nn'

Cuts at:      0     644     981    1482 
   Size:        644     337     501

  Fragments arranged by size: 

                 644     501     337
 
DraIII CAC_nnn'GTG

Cuts at:      0    1077    1401    1482 
   Size:       1077     324      81

  Fragments arranged by size: 

                1077     324      81
 
EaeI y'GGCC_r

Cuts at:      0     459     491    1254    1482 
   Size:        459      32     763     228

  Fragments arranged by size: 

                 763     459     228      32
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1030    1482 
   Size:       1030     452
 
EcoICRI GAG'CTC

Cuts at:      0     942    1482 
   Size:        942     540
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     385     387     674     696    1482 
   Size:        296      89       2     287      22     786

  Fragments arranged by size: 

                 786     296     287      89      22       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1059    1482 
   Size:       1059     423
 
HincII GTy'rAC

Cuts at:      0     977    1482 
   Size:        977     505
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     884    1426    1482 
   Size:        884     542      56

  Fragments arranged by size: 

                 884     542      56
 
MscI TGG'CCA

Cuts at:      0     493    1482 
   Size:        493     989
 
MspA1I CmG'CkG

Cuts at:      0     693    1005    1482 
   Size:        693     312     477

  Fragments arranged by size: 

                 693     477     312
 
NspI r_CATG'y

Cuts at:      0     126    1482 
   Size:        126    1356
 
PciI A'CATG_T

Cuts at:      0     122    1482 
   Size:        122    1360
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     846    1445    1482 
   Size:        846     599      37

  Fragments arranged by size: 

                 846     599      37
 
PmlI CAC'GTG

Cuts at:      0     594    1482 
   Size:        594     888
 
PpuMI rG'GwC_Cy

Cuts at:      0    1059    1482 
   Size:       1059     423
 
PvuII CAG'CTG

Cuts at:      0     693    1482 
   Size:        693     789
 
SacI G_AGCT'C

Cuts at:      0     944    1482 
   Size:        944     538
 
SanDI GG'GwC_CC

Cuts at:      0    1059    1482 
   Size:       1059     423
 
SmlI C'TyrA_G

Cuts at:      0     829    1482 
   Size:        829     653
 
SnaBI TAC'GTA

Cuts at:      0     238    1482 
   Size:        238    1244
 
SpeI A'CTAG_T

Cuts at:      0     721    1482 
   Size:        721     761
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1015    1482 
   Size:       1015     467
 
TatI w'GTAC_w

Cuts at:      0     166     452    1482 
   Size:        166     286    1030

  Fragments arranged by size: 

                1030     286     166
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1124    1156    1482 
   Size:       1124      32     326

  Fragments arranged by size: 

                1124     326      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1084    1482 
   Size:       1084     398
 
ZraI GAC'GTC

Cuts at:      0     935    1482 
   Size:        935     547
 

 Enzymes that do cut:

    AatII     AclI     AcuI   AflIII    AlwNI     ApoI     AvaI    BanII
     BbsI     BcgI     BglI Bme1580I    BmgBI     BpmI    BpuEI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BsrDI    BsrFI    BssSI   BstEII    BstYI     BtgI    BtgZI
     BtsI   DraIII     EaeI     EciI  EcoICRI  Eco57MI EcoO109I   HincII
     MmeI     MscI   MspA1I     NspI     PciI    PflMI     PmlI    PpuMI
    PvuII     SacI    SanDI     SmlI    SnaBI     SpeI    TaqII     TatI
     TstI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI     BclI    BfrBI
    BglII     BlpI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaBI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI     DrdI     EagI     EarI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SphI     SrfI
     SspI     StuI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI
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