Restriction map for pF1KA0953
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
Alw44I      ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AviII       AvrII       BbrPI       
BcgI        BclI        BfrI        BglI        BglII       
BlnI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspLU11I    BsrBI       BsrGI       Bst1107I    
BstAPI      BstEII      BstPI       BstZ17I     DraIII      
EagI        EclXI       Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        MfeI        MluI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        SalI        SapI        SfiI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccI         1756  GTCTAC               GT^[AC][GT]AC
AccIII       2127  TCCGGA               T^CCGGA
AflIII        215  ACGTGT               A^C[GA][CT]GT
AhdI          391  GACACCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI           70  CCTGAGG              CC^T[ACGT]AGG
ApaI          985  GGGCCC               GGGCC^C
AspEI         391  GACACCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1540  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          308  TGGCCA               TGG^CCA
BamHI          75  GGATCC               G^GATCC
BbeI          160  GGCGCC               GGCGC^C
BciVI        1312  GTATCC               GTATCC(6/5)
BlpI         1032  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1032  GCTCAGC              GC^T[ACGT]AGC
BsaAI         214  TACGTG               [CT]AC^GT[GA]
BseAI        2127  TCCGGA               T^CCGGA
BspDI         203  ATCGAT               AT^CGAT
BspEI        2127  TCCGGA               T^CCGGA
BsrDI         222  CATTGC               GCAATG(2/0)
BssHII        924  GCGCGC               G^CGCGC
BssSI        1105  CACGAG               CACGAG(-5/-1)
BstBI         488  TTCGAA               TT^CGAA
Bsu36I         70  CCTGAGG              CC^T[ACGT]AGG
CelII        1032  GCTCAGC              GC^T[ACGT]AGC
ClaI          203  ATCGAT               AT^CGAT
CpoI         2012  CGGACCG              CG^G[AT]CCG
DraI          823  TTTAAA               TTT^AAA
Eam1105I      391  GACACCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I         70  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       800  CCAAGG               C^C[AT][AT]GG
HgaI          895  GACGC                GACGC(5/10)
HincII         55  GTTGAC               GT[CT]^[GA]AC
HindII         55  GTTGAC               GT[CT]^[GA]AC
KasI          160  GGCGCC               G^GCGCC
MluNI         308  TGGCCA               TGG^CCA
MroI         2127  TCCGGA               T^CCGGA
MscI          308  TGGCCA               TGG^CCA
MseI          824  TTAA                 T^TAA
NarI          160  GGCGCC               GG^CGCC
NspV          488  TTCGAA               TT^CGAA
PaeR7I        453  CTCGAG               C^TCGAG
PflFI        1540  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         190  GACGTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        2012  CGGACCG              CG^G[AT]CCG
SacI         1954  GAGCTC               GAGCT^C
ScaI          993  AGTACT               AGT^ACT
SexAI         863  ACCTGGT              A^CC[AT]GGT
SfoI          160  GGCGCC               GGC^GCC
SfuI          488  TTCGAA               TT^CGAA
StuI          507  AGGCCT               AGG^CCT
StyI          800  CCAAGG               C^C[AT][AT]GG
Tru9I         824  TTAA                 T^TAA
Tth111I      1540  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1422  TCTAGA               T^CTAGA
XhoI          453  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AcsI          485  AAATTC               [GA]^AATT[CT]
             1406  AAATTC              
AcyI          160  GGCGCC               G[GA]^CG[CT]C
              895  GACGCC              
AlwNI         877  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1823  CAGTGCCTG           
Aor51HI       910  AGCGCT               AGC^GCT
             1178  AGCGCT              
ApoI          485  AAATTC               [GA]^AATT[CT]
             1406  AAATTC              
BbvCI        2038  CCTCAGC              CCTCAGC(-5/-2)
             1017  GCTGAGG             
BcnI          723  CCGGG                CC^[GC]GG
             1055  CCGGG               
BfaI         1355  CTAG                 C^TAG
             1423  CTAG                
BsaBI        1428  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2050  GATGAGGATC          
BsaHI         160  GGCGCC               G[GA]^CG[CT]C
              895  GACGCC              
BsaWI         407  ACCGGA               [AT]^CCGG[AT]
             2127  TCCGGA              
BsgI         1228  CTGCAC               GTGCAG(16/14)
             1615  GTGCAG              
BspHI        1202  TCATGA               T^CATGA
             1550  TCATGA              
BsrI          996  ACTGG                ACTGG(1/-1)
             1326  CCAGT               
BstYI          75  GGATCC               [GA]^GATC[CT]
              344  AGATCC              
DrdI         1066  GACGGGGCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1495  GACATCTCTGTC        
EaeI          308  TGGCCA               [CT]^GGCC[GA]
             1219  CGGCCA              
Eco47III      910  AGCGCT               AGC^GCT
             1178  AGCGCT              
Hin1I         160  GGCGCC               G[GA]^CG[CT]C
              895  GACGCC              
MaeI         1355  CTAG                 C^TAG
             1423  CTAG                
MaeIII        188  GTGAC                ^GT[ACGT]AC
             1493  GTGAC               
MamI         1428  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2050  GATGAGGATC          
MflI           75  GGATCC               [GA]^GATC[CT]
              344  AGATCC              
NciI          723  CCGGG                CC^[GC]GG
             1055  CCGGG               
PflMI         554  CCAATATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1233  CCAGGCGGTGG         
PleI         1412  GACTC                GAGTC(4/5)
              322  GAGTC               
PpuMI         563  GGGACCC              [GA]G^G[AT]CC[CT]
             1211  AGGTCCC             
RcaI         1202  TCATGA               T^CATGA
             1550  TCATGA              
SbfI         1305  CCTGCAGG             CCTGCA^GG
             2097  CCTGCAGG            
SmlI          298  CTCAAG               C^T[CT][GA]AG
              453  CTCGAG              
Sse8387I     1305  CCTGCAGG             CCTGCA^GG
             2097  CCTGCAGG            
Tsp45I        188  GTGAC                ^GT[GC]AC
             1493  GTGAC               
Van91I        554  CCAATATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1233  CCAGGCGGTGG         
XcmI          971  CCACCGGCTCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1382  CCACCGCCCTCATGG     
XhoII          75  GGATCC               [GA]^GATC[CT]
              344  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BssKI       BstF5I      BstNI       
BstUI       BstXI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EarI        EcoNI       EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     KspI        MaeII       MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PstI        PvuII       RsaI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        TseI        Tsp509I     
TspRI       TthHB8I     
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