Restriction map for pF1KA0953
 (Linear) (Six-base) MAPSORT of: KIAA0953.seq  Check: 9832  from: 1  to: 2184



KIAA0953.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2091    2184 
   Size:       2091      93
 
AccI GT'mk_AC

Cuts at:      0    1757    2184 
   Size:       1757     427
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1528    2184 
   Size:       1528     656
 
AfeI AGC'GCT

Cuts at:      0     912    1180    2184 
   Size:        912     268    1004

  Fragments arranged by size: 

                1004     912     268
 
AflIII A'CryG_T

Cuts at:      0     215    2184 
   Size:        215    1969
 
AhdI GACnn_n'nnGTC

Cuts at:      0     396    2184 
   Size:        396    1788
 
AleI CACnn'nnGTG

Cuts at:      0    1822    2184 
   Size:       1822     362
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1102    1134    2184 
   Size:       1102      32    1050

  Fragments arranged by size: 

                1102    1050      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     882    1828    2184 
   Size:        882     946     356

  Fragments arranged by size: 

                 946     882     356
 
ApaI G_GGCC'C

Cuts at:      0     989    2184 
   Size:        989    1195
 
ApoI r'AATT_y

Cuts at:      0     485    1406    2184 
   Size:        485     921     778

  Fragments arranged by size: 

                 921     778     485
 
AvaI C'yCGr_G

Cuts at:      0      79     453    1894    2184 
   Size:         79     374    1441     290

  Fragments arranged by size: 

                1441     374     290      79
 
BamHI G'GATC_C

Cuts at:      0      75    2184 
   Size:         75    2109
 
BanI G'GyrC_C

Cuts at:      0      25     160     352    1084    2184 
   Size:         25     135     192     732    1100

  Fragments arranged by size: 

                1100     732     192     135      25
 
BanII G_rGCy'C

Cuts at:      0     682     989    1150    1958    2184 
   Size:        682     307     161     808     226

  Fragments arranged by size: 

                 808     682     307     226     161
 
BbeI G_GCGC'C

Cuts at:      0     164    2184 
   Size:        164    2020
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     103     535     697    1539    2184 
   Size:        103     432     162     842     645

  Fragments arranged by size: 

                 842     645     432     162     103
 
BbvCI CC'TCA_GC

Cuts at:      0    1018    2039    2184 
   Size:       1018    1021     145

  Fragments arranged by size: 

                1021    1018     145
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1323    2184 
   Size:       1323     861
 
BlpI GC'TnA_GC

Cuts at:      0    1033    2184 
   Size:       1033    1151
 
Bme1580I G_kGCm'C

Cuts at:      0      30     989    1773    2184 
   Size:         30     959     784     411

  Fragments arranged by size: 

                 959     784     411      30
 
BmgBI CAC'GTC

Cuts at:      0     192    1725    2184 
   Size:        192    1533     459

  Fragments arranged by size: 

                1533     459     192
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     656    2109    2184 
   Size:        656    1453      75

  Fragments arranged by size: 

                1453     656      75
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     340    1018    2184 
   Size:        122     218     678    1166

  Fragments arranged by size: 

                1166     678     218     122
 
Bpu10I CC'TnA_GC

Cuts at:      0    1018    1591    2039    2184 
   Size:       1018     573     448     145

  Fragments arranged by size: 

                1018     573     448     145
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283    2184 
   Size:        283    1901
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     644    1855    2080    2184 
   Size:        644    1211     225     104

  Fragments arranged by size: 

                1211     644     225     104
 
BsaAI yAC'GTr

Cuts at:      0     216    2184 
   Size:        216    1968
 
BsaBI GATnn'nnATC

Cuts at:      0    1432    2054    2184 
   Size:       1432     622     130

  Fragments arranged by size: 

                1432     622     130
 
BsaHI Gr'CG_yC

Cuts at:      0     161     896    2184 
   Size:        161     735    1288

  Fragments arranged by size: 

                1288     735     161
 
BsaWI w'CCGG_w

Cuts at:      0     407    2127    2184 
   Size:        407    1720      57

  Fragments arranged by size: 

                1720     407      57
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1102    1132    1165    1195    2184 
   Size:       1102      30      33      30     989

  Fragments arranged by size: 

                1102     989      33      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     400     637    1123    1693    1756    1948    2085    2088 
   Size:        400     237     486     570      63     192     137       3

Cuts at:   2088    2152    2184 
   Size:         64      32

  Fragments arranged by size: 

                 570     486     400     237     192     137      64      63
                  32       3
 
BseYI C'CCAG_C

Cuts at:      0     717    1565    2184 
   Size:        717     848     619

  Fragments arranged by size: 

                 848     717     619
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1213    1636    2184 
   Size:       1213     423     548

  Fragments arranged by size: 

                1213     548     423
 
BsiHKAI G_wGCw'C

Cuts at:      0    1106    1531    1958    2184 
   Size:       1106     425     427     226

  Fragments arranged by size: 

                1106     427     425     226
 
Bsp1286I G_dGCh'C

Cuts at:      0      30     682     989    1106    1150    1531    1773    1958 
   Size:         30     652     307     117      44     381     242     185

Cuts at:   1958    2184 
   Size:        226

  Fragments arranged by size: 

                 652     381     307     242     226     185     117      44
                  30
 
BspEI T'CCGG_A

Cuts at:      0    2127    2184 
   Size:       2127      57
 
BspHI T'CATG_A

Cuts at:      0    1202    1550    2184 
   Size:       1202     348     634

  Fragments arranged by size: 

                1202     634     348
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     347    1299    2091    2184 
   Size:        347     952     792      93

  Fragments arranged by size: 

                 952     792     347      93
 
BsrDI GCAATG_nn'

Cuts at:      0     221    2184 
   Size:        221    1963
 
BsrFI r'CCGG_y

Cuts at:      0     635     973    2015    2184 
   Size:        635     338    1042     169

  Fragments arranged by size: 

                1042     635     338     169
 
BssHII G'CGCG_C

Cuts at:      0     924    2184 
   Size:        924    1260
 
BssSI C'ACGA_G

Cuts at:      0    1105    2184 
   Size:       1105    1079
 
BstBI TT'CG_AA

Cuts at:      0     489    2184 
   Size:        489    1695
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     208     318     996    2163    2184 
   Size:        208     110     678    1167      21

  Fragments arranged by size: 

                1167     678     208     110      21
 
BstYI r'GATC_y

Cuts at:      0      75     344    2184 
   Size:         75     269    1840

  Fragments arranged by size: 

                1840     269      75
 
Bsu36I CC'TnA_GG

Cuts at:      0      71    2184 
   Size:         71    2113
 
BtgI C'CryG_G

Cuts at:      0     683     956    1142    1216    2184 
   Size:        683     273     186      74     968

  Fragments arranged by size: 

                 968     683     273     186      74
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     948    2184 
   Size:        948    1236
 
BtsI GCAGTG_nn'

Cuts at:      0     261     595    2004    2184 
   Size:        261     334    1409     180

  Fragments arranged by size: 

                1409     334     261     180
 
ClaI AT'CG_AT

Cuts at:      0     204    2184 
   Size:        204    1980
 
DraI TTT'AAA

Cuts at:      0     825    2184 
   Size:        825    1359
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1072    1501    2184 
   Size:       1072     429     683

  Fragments arranged by size: 

                1072     683     429
 
EaeI y'GGCC_r

Cuts at:      0     308    1219    2184 
   Size:        308     911     965

  Fragments arranged by size: 

                 965     911     308
 
EarI CTCTTCn'nnn_

Cuts at:      0     286    1201    1966    2184 
   Size:        286     915     765     218

  Fragments arranged by size: 

                 915     765     286     218
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1954    2184 
   Size:       1954     230
 
EcoICRI GAG'CTC

Cuts at:      0    1956    2184 
   Size:       1956     228
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     340    1018    1528    2184 
   Size:        122     218     678     510     656

  Fragments arranged by size: 

                 678     656     510     218     122
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1231    1306    1393    2184 
   Size:       1231      75      87     791

  Fragments arranged by size: 

                1231     791      87      75
 
EcoO109I rG'GnC_Cy

Cuts at:      0     564     985    1212    2184 
   Size:        564     421     227     972

  Fragments arranged by size: 

                 972     564     421     227
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     135    2184 
   Size:        135    2049
 
HaeII r_GCGC'y

Cuts at:      0     164     914    1182    1636    2184 
   Size:        164     750     268     454     548

  Fragments arranged by size: 

                 750     548     454     268     164
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     382     414     622     654    1513    1545    2047    2075 
   Size:        382      32     208      32     859      32     502      28

Cuts at:   2075    2079    2098    2107    2109    2130    2141    2184 
   Size:          4      19       9       2      21      11      43

  Fragments arranged by size: 

                 859     502     382     208      43      32      32      32
                  28      21      19      11       9       4       2
 
HincII GTy'rAC

Cuts at:      0      57    2184 
   Size:         57    2127
 
KasI G'GCGC_C

Cuts at:      0     160    2184 
   Size:        160    2024
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     929    2184 
   Size:        929    1255
 
MscI TGG'CCA

Cuts at:      0     310    2184 
   Size:        310    1874
 
MslI CAynn'nnrTG

Cuts at:      0     206     226    1822    1847    2184 
   Size:        206      20    1596      25     337

  Fragments arranged by size: 

                1596     337     206      25      20
 
MspA1I CmG'CkG

Cuts at:      0     246     685     958    1026    1218    1766    2043    2184 
   Size:        246     439     273      68     192     548     277     141

  Fragments arranged by size: 

                 548     439     277     273     246     192     141      68
            
 
NarI GG'CG_CC

Cuts at:      0     161    2184 
   Size:        161    2023
 
NspI r_CATG'y

Cuts at:      0     619    1119    1890    2184 
   Size:        619     500     771     294

  Fragments arranged by size: 

                 771     619     500     294
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     560    1239    2184 
   Size:        560     679     945

  Fragments arranged by size: 

                 945     679     560
 
PfoI T'CCnGG_A

Cuts at:      0    1121    1364    2184 
   Size:       1121     243     820

  Fragments arranged by size: 

                1121     820     243
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1102    1134    2184 
   Size:       1102      32    1050

  Fragments arranged by size: 

                1102    1050      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     564    1212    2184 
   Size:        564     648     972

  Fragments arranged by size: 

                 972     648     564
 
PshAI GACnn'nnGTC

Cuts at:      0     194    2184 
   Size:        194    1990
 
PspOMI G'GGCC_C

Cuts at:      0     985    2184 
   Size:        985    1199
 
PspXI vC'TCGA_Gb

Cuts at:      0     453    2184 
   Size:        453    1731
 
PstI C_TGCA'G

Cuts at:      0     344     551    1310    2102    2184 
   Size:        344     207     759     792      82

  Fragments arranged by size: 

                 792     759     344     207      82
 
PvuII CAG'CTG

Cuts at:      0     246    1026    2043    2184 
   Size:        246     780    1017     141

  Fragments arranged by size: 

                1017     780     246     141
 
RsrII CG'GwC_CG

Cuts at:      0    2013    2184 
   Size:       2013     171
 
SacI G_AGCT'C

Cuts at:      0    1958    2184 
   Size:       1958     226
 
SacII CC_GC'GG

Cuts at:      0     686     959    1219    2184 
   Size:        686     273     260     965

  Fragments arranged by size: 

                 965     686     273     260
 
SanDI GG'GwC_CC

Cuts at:      0     564    2184 
   Size:        564    1620
 
SbfI CC_TGCA'GG

Cuts at:      0    1310    2102    2184 
   Size:       1310     792      82

  Fragments arranged by size: 

                1310     792      82
 
ScaI AGT'ACT

Cuts at:      0     995    2184 
   Size:        995    1189
 
SexAI A'CCwGG_T

Cuts at:      0     863    2184 
   Size:        863    1321
 
SfcI C'TryA_G

Cuts at:      0     340     547     610    1306    1575    2098    2184 
   Size:        340     207      63     696     269     523      86

  Fragments arranged by size: 

                 696     523     340     269     207      86      63
 
SfoI GGC'GCC

Cuts at:      0     162    2184 
   Size:        162    2022
 
SmlI C'TyrA_G

Cuts at:      0     298     453    2184 
   Size:        298     155    1731

  Fragments arranged by size: 

                1731     298     155
 
StuI AGG'CCT

Cuts at:      0     509    2184 
   Size:        509    1675
 
StyI C'CwwG_G

Cuts at:      0     800    2184 
   Size:        800    1384
 
TatI w'GTAC_w

Cuts at:      0     836     993    2184 
   Size:        836     157    1191

  Fragments arranged by size: 

                1191     836     157
 
Tth111I GACn'n_nGTC

Cuts at:      0    1543    2184 
   Size:       1543     641
 
XbaI T'CTAG_A

Cuts at:      0    1422    2184 
   Size:       1422     762
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     978    1389    2184 
   Size:        978     411     795

  Fragments arranged by size: 

                 978     795     411
 
XhoI C'TCGA_G

Cuts at:      0     453    2184 
   Size:        453    1731
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AloI
    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BlpI Bme1580I    BmgBI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI   BssHII    BssSI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
   HincII     KasI     MmeI     MscI     MslI   MspA1I     NarI     NspI
    PflMI     PfoI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
    PvuII    RsrII     SacI    SacII    SanDI     SbfI     ScaI    SexAI
     SfcI     SfoI     SmlI     StuI     StyI     TatI  Tth111I     XbaI
     XcmI     XhoI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AlfI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI     BcgI     BclI    BfrBI     BglI
    BglII     BmrI     BmtI    BsiEI    BsiWI     BsmI    BsmBI    BsrBI
    BsrGI   BstAPI   BstEII  BstZ17I    CspCI   DraIII     EagI    EcoRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PsiI     PsrI
     PvuI     SalI     SapI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TstI     XmaI
     XmnI     ZraI
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