Restriction map for pF1KA0946
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# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
Alw44I      AocI        ApaI        ApaLI       AscI        
Asp700I     AspHI       AvaI        AviII       AvrII       
BalI        BanII       BbeI        BbsI        BbvCI       
BcgI        BciVI       BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
BstAPI      BstEII      BstPI       BstUI       Bsu36I      
CelII       ClaI        CpoI        DrdI        EaeI        
EagI        EclXI       Eco52I      Eco81I      EcoNI       
EcoO65I     FseI        FspI        HindIII     KasI        
KspI        MamI        MluI        MluNI       MroI        
MscI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NspI        PacI        
PaeR7I      PmeI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
XbaI        XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   68 enzymes) #
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Acc65I       1883  GGTACC               G^GTACC
AccI         1848  GTATAC               GT^[AC][GT]AC
AcyI          573  GACGCC               G[GA]^CG[CT]C
AflIII       1576  ACGTGT               A^C[GA][CT]GT
AgeI         1338  ACCGGT               A^CCGGT
Aor51HI       780  AGCGCT               AGC^GCT
AseI          453  ATTAAT               AT^TAAT
AsnI          453  ATTAAT               AT^TAAT
Asp718I      1883  GGTACC               G^GTACC
AspI          178  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         612  GGATCC               G^GATCC
BanI         1883  GGTACC               G^G[CT][GA]CC
BbrPI        1575  CACGTG               CAC^GTG
BcnI           42  CCCGG                CC^[GC]GG
BmyI           73  GTGCCC               G[AGT]GC[ACT]^C
BpmI          740  CTGGAG               CTGGAG(16/14)
BsaAI        1575  CACGTG               [CT]AC^GT[GA]
BsaHI         573  GACGCC               G[GA]^CG[CT]C
BsaI         1525  GAGACC               GGTCTC(1/5)
BsmI         1701  GAATGC               GAATGC(1/-1)
Bsp1286I       73  GTGCCC               G[AGT]GC[ACT]^C
BspMI         235  GCAGGT               ACCTGC(4/8)
BsrFI        1338  ACCGGT               [GA]^CCGG[CT]
Bst1107I     1848  GTATAC               GTA^TAC
BstBI         330  TTCGAA               TT^CGAA
BstZ17I      1848  GTATAC               GTA^TAC
CfoI          781  GCGC                 GCG^C
Cfr10I       1338  ACCGGT               [GA]^CCGG[CT]
DraI         1723  TTTAAA               TTT^AAA
DraII         362  GGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII        602  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         1249  GAAGAG               CTCTTC(1/4)
Eco47III      780  AGCGCT               AGC^GCT
EcoO109I      362  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         369  GAATTC               G^AATTC
EcoRV        1665  GATATC               GAT^ATC
EcoT22I      1060  ATGCAT               ATGCA^T
HaeII         780  AGCGCT               [GA]GCGC^[CT]
HgaI          573  GACGC                GACGC(5/10)
HhaI          781  GCGC                 GCG^C
Hin1I         573  GACGCC               G[GA]^CG[CT]C
HinP1I        781  GCGC                 G^CGC
HincII        649  GTTAAC               GT[CT]^[GA]AC
HindII        649  GTTAAC               GT[CT]^[GA]AC
HpaI          649  GTTAAC               GTT^AAC
KpnI         1883  GGTACC               GGTAC^C
Ksp632I      1249  GAAGAG               CTCTTC(1/4)
MfeI          512  CAATTG               C^AATTG
MunI          512  CAATTG               C^AATTG
NciI           42  CCCGG                CC^[GC]GG
NheI          699  GCTAGC               G^CTAGC
NsiI         1060  ATGCAT               ATGCA^T
NspV          330  TTCGAA               TT^CGAA
PflFI         178  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1097  CCAAATTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1338  ACCGGT               A^CCGGT
PleI         1514  GACTC                GAGTC(4/5)
PmaCI        1575  CACGTG               CAC^GTG
PmlI         1575  CACGTG               CAC^GTG
PpuMI         362  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI         453  ATTAAT               AT^TAAT
PstI         1314  CTGCAG               CTGCA^G
PvuII        1888  CAGCTG               CAG^CTG
SapI         1249  GAAGAGC              GCTCTTC(1/4)
SfuI          330  TTCGAA               TT^CGAA
Tth111I       178  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1097  CCAAATTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          111  CCATTATAACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AhdI          859  GACAATCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1287  GACAGGAAGTC         
AlwNI        1317  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1874  CAGGATCTG           
AspEI         859  GACAATCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1287  GACAGGAAGTC         
BclI          818  TGATCA               T^GATCA
             1389  TGATCA              
BsaWI         255  ACCGGA               [AT]^CCGG[AT]
             1338  ACCGGT              
BseRI         277  CTCCTC               GAGGAG(10/8)
               10  GAGGAG              
BsgI          440  GTGCAG               GTGCAG(16/14)
             1424  GTGCAG              
BsmAI        1285  GAGAC                GTCTC(1/5)
             1525  GAGAC               
BstXI         120  CCATGGATATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              379  CCATTGCACTGG        
Eam1105I      859  GACAATCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1287  GACAGGAAGTC         
FauI            6  GCGGG                CCCGC(4/6)
               64  GCGGG               
FbaI          818  TGATCA               T^GATCA
             1389  TGATCA              
MwoI          694  GCAGTGCTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              838  GCAAGGCAAGC         
SfaNI         754  GATGC                GCATC(5/9)
             1802  GATGC               
SfcI         1019  CTACAG               C^T[GA][CT]AG
             1314  CTGCAG              
SspI          970  AATATT               AAT^ATT
             1777  AATATT              
TfiI          775  GAATC                G^A[AT]TC
              976  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbvI        BfaI        BsaJI       
BsiYI       BslI        BsmFI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DsaI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        NcoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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