Restriction map for pF1KA0938
 (Circular) (Six-base) MAPSORT of: pF1KA0938.seq  Check: 2143  from: 1  to: 8639



pF1KA0938  8639 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2365    2365 
   Size:       8639
 
AatII G_ACGT'C

Cuts at:   4356    4356 
   Size:       8639
 
AccI GT'mk_AC

Cuts at:    813     949    5677     813 
   Size:        136    4728    3775

  Fragments arranged by size: 

                4728    3775     136
 
Acc65I G'GTAC_C

Cuts at:    907    5655     907 
   Size:       4748    3891
 
AclI AA'CG_TT

Cuts at:   8479    8479 
   Size:       8639
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    157     491     542     844    1535    1706    2905    3272    3496 
   Size:        334      51     302     691     171    1199     367     224

Cuts at:   3496    3821    3907    5293    5398    6464    6896    7249    7911 
   Size:        325      86    1386     105    1066     432     353     662

Cuts at:   7911     157 
   Size:        885

  Fragments arranged by size: 

                1386    1199    1066     885     691     662     432     367
                 353     334     325     302     224     171     105      86
                  51
 
AflII C'TTAA_G

Cuts at:   2580    2580 
   Size:       8639
 
AflIII A'CryG_T

Cuts at:    482     992    1037    5991    7791    8072     482 
   Size:        510      45    4954    1800     281    1049

  Fragments arranged by size: 

                4954    1800    1049     510     281      45
 
AgeI A'CCGG_T

Cuts at:   4028    4028 
   Size:       8639
 
AhdI GACnn_n'nnGTC

Cuts at:   4758    4758 
   Size:       8639
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    134     134 
   Size:       8639
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1306    1338    1306 
   Size:         32    8607
 
AlwNI CAG_nnn'CTG

Cuts at:    342     515    2288    2802    3862    4045    4159    5944    7382 
   Size:        173    1773     514    1060     183     114    1785    1438

Cuts at:   7382     342 
   Size:       1599

  Fragments arranged by size: 

                1785    1773    1599    1438    1060     514     183     173
                 114
 
ApaI G_GGCC'C

Cuts at:   3047    3047 
   Size:       8639
 
ApaLI G'TGCA_C

Cuts at:   1631    2294    7477    1631 
   Size:        663    5183    2793

  Fragments arranged by size: 

                5183    2793     663
 
ApoI r'AATT_y

Cuts at:    690    1892    3018    3486    5643    7992    8248     690 
   Size:       1202    1126     468    2157    2349     256    1081

  Fragments arranged by size: 

                2349    2157    1202    1126    1081     468     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       8639
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8639
 
AvaI C'yCGr_G

Cuts at:    936    1193    1522    1645    2172    3040    5556    5659     936 
   Size:        257     329     123     527     868    2516     103    3916

  Fragments arranged by size: 

                3916    2516     868     527     329     257     123     103
            
 
BamHI G'GATC_C

Cuts at:   2317    4297    5664    2317 
   Size:       1980    1367    5292

  Fragments arranged by size: 

                5292    1980    1367
 
BanI G'GyrC_C

Cuts at:    907    1602    2365    5655    6069    6317    6352     907 
   Size:        695     763    3290     414     248      35    3194

  Fragments arranged by size: 

                3290    3194     763     695     414     248      35
 
BanII G_rGCy'C

Cuts at:   2725    3013    3047    3172    3899    4411    4835    4876    5380 
   Size:        288      34     125     727     512     424      41     504

Cuts at:   5380    5404    5653    6683    2725 
   Size:         24     249    1030    4681

  Fragments arranged by size: 

                4681    1030     727     512     504     424     288     249
                 125      41      34      24
 
BbeI G_GCGC'C

Cuts at:   1606    6073    6321    1606 
   Size:       4467     248    3924

  Fragments arranged by size: 

                4467    3924     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    413     641     706    1034    1498    1523    3567    5140    5299 
   Size:        228      65     328     464      25    2044    1573     159

Cuts at:   5299    5606     413 
   Size:        307    3446

  Fragments arranged by size: 

                3446    2044    1573     464     328     307     228     159
                  65      25
 
BbvCI CC'TCA_GC

Cuts at:   1378    2746    3863    3943    1378 
   Size:       1368    1117      80    6074

  Fragments arranged by size: 

                6074    1368    1117      80
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    8605
 
BciVI GTATCCnnnnn_n'

Cuts at:   2336    2676    4483    6537    7593    8639    2336 
   Size:        340    1807    2054    1056    1046    2336

  Fragments arranged by size: 

                2336    2054    1807    1056    1046     340
 
BclI T'GATC_A

Cuts at:   1841    3963    6159    1841 
   Size:       2122    2196    4321

  Fragments arranged by size: 

                4321    2196    2122
 
BglI GCCn_nnn'nGGC

Cuts at:   1611    2066    2756    3502    4068    1611 
   Size:        455     690     746     566    6182

  Fragments arranged by size: 

                6182     746     690     566     455
 
BglII A'GATC_T

Cuts at:   1714    2827    3546    6154    8191    1714 
   Size:       1113     719    2608    2037    2162

  Fragments arranged by size: 

                2608    2162    2037    1113     719
 
BlpI GC'TnA_GC

Cuts at:    360    2518    3807    4058    5379    5751     360 
   Size:       2158    1289     251    1321     372    3248

  Fragments arranged by size: 

                3248    2158    1321    1289     372     251
 
Bme1580I G_kGCm'C

Cuts at:   1635    2298    3047    6264    6357    7481    1635 
   Size:        663     749    3217      93    1124    2793

  Fragments arranged by size: 

                3217    2793    1124     749     663      93
 
BmgBI CAC'GTC

Cuts at:   5138    5138 
   Size:       8639
 
BmrI ACTGGGnnnn_n'

Cuts at:   1894    4831    5851    5932    6267    8411    1894 
   Size:       2937    1020      81     335    2144    2122

  Fragments arranged by size: 

                2937    2144    2122    1020     335      81
 
BmtI G_CTAG'C

Cuts at:   4427    4427 
   Size:       8639
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    321    1416    2849    4849    7006     321 
   Size:       1095    1433    2000    2157    1954

  Fragments arranged by size: 

                2157    2000    1954    1433    1095
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    766     852    1593    2491    2583    2933    3157    3955    5308 
   Size:         86     741     898      92     350     224     798    1353

Cuts at:   5308     766 
   Size:       4097

  Fragments arranged by size: 

                4097    1353     898     798     741     350     224      92
                  86
 
Bpu10I CC'TnA_GC

Cuts at:   1378    2746    3381    3863    3943    1378 
   Size:       1368     635     482      80    6074

  Fragments arranged by size: 

                6074    1368     635     482      80
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    702    4071    5815    6694    7408    7706     702 
   Size:       3369    1744     879     714     298    1635

  Fragments arranged by size: 

                3369    1744    1635     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    219    1279    1408    2308    2641    8343     219 
   Size:       1060     129     900     333    5702     515

  Fragments arranged by size: 

                5702    1060     900     515     333     129
 
BsaAI yAC'GTr

Cuts at:   5992    5992 
   Size:       8639
 
BsaBI GATnn'nnATC

Cuts at:      6    6153    8190       6 
   Size:       6147    2037     455

  Fragments arranged by size: 

                6147    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1603    4353    6070    6318    7020    8516    1603 
   Size:       2750    1717     248     702    1496    1726

  Fragments arranged by size: 

                2750    1726    1717    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    137    2485    3986    4028    5828    6349    7027    7438    7585 
   Size:       2348    1501      42    1800     521     678     411     147

Cuts at:   7585    7859     137 
   Size:        274     917

  Fragments arranged by size: 

                2348    1800    1501     917     678     521     411     274
                 147      42
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    472     502    2464    2494    3702    3732     472 
   Size:         30    1962      30    1208      30    5379

  Fragments arranged by size: 

                5379    1962    1208      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    237     541     586    2078    4089     237 
   Size:        304      45    1492    2011    4787

  Fragments arranged by size: 

                4787    2011    1492     304      45
 
BseYI C'CCAG_C

Cuts at:    382     515     591     984    1244    2119    2434    2604    3046 
   Size:        133      76     393     260     875     315     170     442

Cuts at:   3046    3072    3110    5403    6064    6822    7487    7895     382 
   Size:         26      38    2293     661     758     665     408    1126

  Fragments arranged by size: 

                2293    1126     875     758     665     661     442     408
                 393     315     260     170     133      76      38      26
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    355    2378    4827    5433     355 
   Size:       2023    2449     606    3561

  Fragments arranged by size: 

                3561    2449    2023     606
 
BsiEI CG_ry'CG

Cuts at:    104    6181    6227    7457     104 
   Size:       6077      46    1230    1286

  Fragments arranged by size: 

                6077    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1635    2298    2725    3519    3899    4411    4835    5380    5563 
   Size:        663     427     794     380     512     424     545     183

Cuts at:   5563    5653    6431    6621    7481    1635 
   Size:         90     778     190     860    2793

  Fragments arranged by size: 

                2793     860     794     778     663     545     512     427
                 424     380     190     183      90
 
BsmI GAATG_Cn'

Cuts at:   2051    3567    3856    2051 
   Size:       1516     289    6834

  Fragments arranged by size: 

                6834    1516     289
 
BsmBI CGTCTCn'nnnn_

Cuts at:   4360    4360 
   Size:       8639
 
Bsp1286I G_dGCh'C

Cuts at:   1635    2298    2725    3013    3047    3172    3519    3899    4411 
   Size:        663     427     288      34     125     347     380     512

Cuts at:   4411    4835    4876    5380    5404    5563    5653    6264    6357 
   Size:        424      41     504      24     159      90     611      93

Cuts at:   6357    6431    6621    6683    7481    1635 
   Size:         74     190      62     798    2793

  Fragments arranged by size: 

                2793     798     663     611     512     504     427     424
                 380     347     288     190     159     125      93      90
                  74      62      41      34      24
 
BspEI T'CCGG_A

Cuts at:    137     137 
   Size:       8639
 
BspHI T'CATG_A

Cuts at:   2807    7071    2807 
   Size:       4264    4375
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2365    5689    5903    6205    6586    2365 
   Size:       3324     214     302     381    4418

  Fragments arranged by size: 

                4418    3324     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     300    6931    6985    8469    8634      50 
   Size:        250    6631      54    1484     165      55

  Fragments arranged by size: 

                6631    1484     250     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   4418    6551    4418 
   Size:       2133    6506
 
BsrFI r'CCGG_y

Cuts at:   4028    6637    6818    4028 
   Size:       2609     181    5849

  Fragments arranged by size: 

                5849    2609     181
 
BssSI C'ACGA_G

Cuts at:   2638    2718    2788    3149    6910    7618    2638 
   Size:         80      70     361    3761     708    3659

  Fragments arranged by size: 

                3761    3659     708     361      80      70
 
BstAPI GCAn_nnn'nTGC

Cuts at:   6016    6016 
   Size:       8639
 
BstBI TT'CG_AA

Cuts at:   2352    5055    7000    2352 
   Size:       2703    1945    3991

  Fragments arranged by size: 

                3991    2703    1945
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3118    3135    3793    3118 
   Size:         17     658    7964

  Fragments arranged by size: 

                7964     658      17
 
BstYI r'GATC_y

Cuts at:      1    1714    2074    2317    2827    3546    3968    4297    4450 
   Size:       1713     360     243     510     719     422     329     153

Cuts at:   4450    5253    5340    5508    5664    6131    6154    6489    6735 
   Size:        803      87     168     156     467      23     335     246

Cuts at:   6735    7041    7053    7139    8191       1 
   Size:        306      12      86    1052     449

  Fragments arranged by size: 

                1713    1052     803     719     510     467     449     422
                 360     335     329     306     246     243     168     156
                 153      87      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    950     950 
   Size:       8639
 
Bsu36I CC'TnA_GG

Cuts at:   1430    1767    5393    1430 
   Size:        337    3626    4676

  Fragments arranged by size: 

                4676    3626     337
 
BtgI C'CryG_G

Cuts at:    230    1467    5219    5332    5371     230 
   Size:       1237    3752     113      39    3498

  Fragments arranged by size: 

                3752    3498    1237     113      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   6589    6770    6933    6589 
   Size:        181     163    8295

  Fragments arranged by size: 

                8295     181     163
 
BtsI GCAGTG_nn'

Cuts at:    909    1032    1275    1746    1857    2511    3630    4227    4815 
   Size:        123     243     471     111     654    1119     597     588

Cuts at:   4815    5300    5882     909 
   Size:        485     582    3666

  Fragments arranged by size: 

                3666    1119     654     597     588     582     485     471
                 243     123     111
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   2358    2393    4380    4415    2358 
   Size:         35    1987      35    6582

  Fragments arranged by size: 

                6582    1987      35      35
 
DraI TTT'AAA

Cuts at:   5638    5638 
   Size:       8639
 
DraIII CAC_nnn'GTG

Cuts at:   1224    1224 
   Size:       8639
 
DrdI GACnn_nn'nnGTC

Cuts at:   6345    7689    6345 
   Size:       1344    7295
 
EaeI y'GGCC_r

Cuts at:   2058    3396    4069    4215    6224    6398    6789    6816    8161 
   Size:       1338     673     146    2009     174     391      27    1345

Cuts at:   8161    2058 
   Size:       2536

  Fragments arranged by size: 

                2536    2009    1345    1338     673     391     174     146
                  27
 
EagI C'GGCC_G

Cuts at:   6224    6224 
   Size:       8639
 
EarI CTCTTCn'nnn_

Cuts at:    426     518     692    1572    2545    2873    3090    3210    3737 
   Size:         92     174     880     973     328     217     120     527

Cuts at:   3737    3883    4107    6662    6872     426 
   Size:        146     224    2555     210    2193

  Fragments arranged by size: 

                2555    2193     973     880     527     328     224     217
                 210     174     146     120      92
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   6847    7589    7735    8449    6847 
   Size:        742     146     714    7037

  Fragments arranged by size: 

                7037     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2723    3897    4409    4833    5378    5651    2723 
   Size:       1174     512     424     545     273    5711

  Fragments arranged by size: 

                5711    1174     545     512     424     273
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    157     491     542     766     844     852    1535    1593    1706 
   Size:        334      51     224      78       8     683      58     113

Cuts at:   1706    2491    2583    2905    2933    3157    3272    3496    3821 
   Size:        785      92     322      28     224     115     224     325

Cuts at:   3821    3907    3955    5293    5308    5398    6464    6896    7249 
   Size:         86      48    1338      15      90    1066     432     353

Cuts at:   7249    7911     157 
   Size:        662     885

  Fragments arranged by size: 

                1338    1066     885     785     683     662     432     353
                 334     325     322     224     224     224     115     113
                  92      90      86      78      58      51      48      28
                  15       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2060    2060 
   Size:       8639
 
EcoO109I rG'GnC_Cy

Cuts at:   3939    5778    3939 
   Size:       1839    6800
 
EcoRI G'AATT_C

Cuts at:   3486    5643    3486 
   Size:       2157    6482
 
EcoRV GAT'ATC

Cuts at:   1083    4887    5050    1083 
   Size:       3804     163    4672

  Fragments arranged by size: 

                4672    3804     163
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1736    2261    6658    1736 
   Size:        525    4397    3717

  Fragments arranged by size: 

                4397    3717     525
 
FspI TGC'GCA

Cuts at:    238    6420     238 
   Size:       6182    2457
 
FspAI rTGC'GCAy

Cuts at:    238     238 
   Size:       8639
 
HaeII r_GCGC'y

Cuts at:    306    1606    2172    2288    6073    6321    7551    8322     306 
   Size:       1300     566     116    3785     248    1230     771     623

  Fragments arranged by size: 

                3785    1300    1230     771     623     566     248     116
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    406     438     451     483     546     578     691     723     862 
   Size:         32      13      32      63      32     113      32     139

Cuts at:    862     894     959     991    1257    1289    2323    2355    2464 
   Size:         32      65      32     266      32    1034      32     109

Cuts at:   2464    2496    2819    2851    3625    3657    3790    3822    4261 
   Size:         32     323      32     774      32     133      32     439

Cuts at:   4261    4293    4608    4640    4744    4776    5127    5159    5289 
   Size:         32     315      32     104      32     351      32     130

Cuts at:   5289    5321    6481    6511    6513    6543     406 
   Size:         32    1160      30       2      30    2502

  Fragments arranged by size: 

                2502    1160    1034     774     439     351     323     315
                 266     139     133     130     113     109     104      65
                  63      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      30      30      13       2
 
HincII GTy'rAC

Cuts at:    814    2190    5678     814 
   Size:       1376    3488    3775

  Fragments arranged by size: 

                3775    3488    1376
 
HindIII A'AGCT_T

Cuts at:   1541    1667    2577    1541 
   Size:        126     910    7603

  Fragments arranged by size: 

                7603     910     126
 
KasI G'GCGC_C

Cuts at:   1602    6069    6317    1602 
   Size:       4467     248    3924

  Fragments arranged by size: 

                4467    3924     248
 
KpnI G_GTAC'C

Cuts at:    911    5659     911 
   Size:       4748    3891
 
MfeI C'AATT_G

Cuts at:   4733    4905    4733 
   Size:        172    8467
 
MluI A'CGCG_T

Cuts at:   8072    8072 
   Size:       8639
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    269    1367    2123    4479    7398    7582     269 
   Size:       1098     756    2356    2919     184    1326

  Fragments arranged by size: 

                2919    2356    1326    1098     756     184
 
MscI TGG'CCA

Cuts at:   6400    6400 
   Size:       8639
 
MslI CAynn'nnrTG

Cuts at:    566     823    1472    3469    4211    4519    5370    6628    6755 
   Size:        257     649    1997     742     308     851    1258     127

Cuts at:   6755     566 
   Size:       2450

  Fragments arranged by size: 

                2450    1997    1258     851     742     649     308     257
                 127
 
MspA1I CmG'CkG

Cuts at:    114    1244    1377    2980    3250    3424    3885    5418    5750 
   Size:       1130     133    1603     270     174     461    1533     332

Cuts at:   5750    6064    6424    7208    7453     114 
   Size:        314     360     784     245    1300

  Fragments arranged by size: 

                1603    1533    1300    1130     784     461     360     332
                 314     270     245     174     133
 
NaeI GCC'GGC

Cuts at:   6820    6820 
   Size:       8639
 
NarI GG'CG_CC

Cuts at:   1603    6070    6318    1603 
   Size:       4467     248    3924

  Fragments arranged by size: 

                4467    3924     248
 
NcoI C'CATG_G

Cuts at:   1467    5219    5332    5371    1467 
   Size:       3752     113      39    4735

  Fragments arranged by size: 

                4735    3752     113      39
 
NgoMIV G'CCGG_C

Cuts at:   6818    6818 
   Size:       8639
 
NheI G'CTAG_C

Cuts at:   4423    4423 
   Size:       8639
 
NspI r_CATG'y

Cuts at:    851     996    1041    1587    1987    5692    6192    7795     851 
   Size:        145      45     546     400    3705     500    1603    1695

  Fragments arranged by size: 

                3705    1695    1603     546     500     400     145      45
            
 
PasI CC'CwG_GG

Cuts at:    882     882 
   Size:       8639
 
PciI A'CATG_T

Cuts at:    992    1037    7791     992 
   Size:         45    6754    1840

  Fragments arranged by size: 

                6754    1840      45
 
PflMI CCAn_nnn'nTGG

Cuts at:    830     830 
   Size:       8639
 
PfoI T'CCnGG_A

Cuts at:   1570    3971    1570 
   Size:       2401    6238
 
PmeI GTTT'AAAC

Cuts at:   5638    5638 
   Size:       8639
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   7085    7117    7085 
   Size:         32    8607
 
PsiI TTA'TAA

Cuts at:   2575    2575 
   Size:       8639
 
PspOMI G'GGCC_C

Cuts at:   3043    3043 
   Size:       8639
 
PspXI vC'TCGA_Gb

Cuts at:    936    2172    5556     936 
   Size:       1236    3384    4019

  Fragments arranged by size: 

                4019    3384    1236
 
PstI C_TGCA'G

Cuts at:   2673    2979    3884    5686    6371    2673 
   Size:        306     905    1802     685    4941

  Fragments arranged by size: 

                4941    1802     905     685     306
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       8639
 
PvuII CAG'CTG

Cuts at:   1244    1377    2980    3250    3885    5418    6064    6424    1244 
   Size:        133    1603     270     635    1533     646     360    3459

  Fragments arranged by size: 

                3459    1603    1533     646     635     360     270     133
            
 
RsrII CG'GwC_CG

Cuts at:   6834    6834 
   Size:       8639
 
SacI G_AGCT'C

Cuts at:   2725    3899    4411    4835    5380    5653    2725 
   Size:       1174     512     424     545     273    5711

  Fragments arranged by size: 

                5711    1174     545     512     424     273
 
SalI G'TCGA_C

Cuts at:    812    5676     812 
   Size:       4864    3775
 
SapI GCTCTTCn'nnn_

Cuts at:   3210    3737    3883    6662    6872    3210 
   Size:        527     146    2779     210    4977

  Fragments arranged by size: 

                4977    2779     527     210     146
 
SbfI CC_TGCA'GG

Cuts at:   5686    5686 
   Size:       8639
 
ScaI AGT'ACT

Cuts at:   8156    8156 
   Size:       8639
 
SfcI C'TryA_G

Cuts at:     33    2564    2669    2975    3245    3880    5682    6367    7335 
   Size:       2531     105     306     270     635    1802     685     968

Cuts at:   7335    7526      33 
   Size:        191    1146

  Fragments arranged by size: 

                2531    1802    1146     968     685     635     306     270
                 191     105
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2066    2066 
   Size:       8639
 
SfoI GGC'GCC

Cuts at:   1604    6071    6319    1604 
   Size:       4467     248    3924

  Fragments arranged by size: 

                4467    3924     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8639
 
SmaI CCC'GGG

Cuts at:   1195    1647    3042    5661    1195 
   Size:        452    1395    2619    4173

  Fragments arranged by size: 

                4173    2619    1395     452
 
SmlI C'TyrA_G

Cuts at:    681     936    2172    2580    4086    5556    5794    6709    7423 
   Size:        255    1236     408    1506    1470     238     915     714

Cuts at:   7423    7685     681 
   Size:        262    1635

  Fragments arranged by size: 

                1635    1506    1470    1236     915     714     408     262
                 255     238
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       8639
 
SphI G_CATG'C

Cuts at:   5692    6192    5692 
   Size:        500    8139
 
StyI C'CwwG_G

Cuts at:    266    1086    1467    1593    2369    4398    5219    5332    5371 
   Size:        820     381     126     776    2029     821     113      39

Cuts at:   5371    5773    6127     266 
   Size:        402     354    2778

  Fragments arranged by size: 

                2778    2029     821     820     776     402     381     354
                 126     113      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    583     874    1048    2358    4662    5343    6222    6355    6814 
   Size:        291     174    1310    2304     681     879     133     459

Cuts at:   6814    7023     583 
   Size:        209    2199

  Fragments arranged by size: 

                2304    2199    1310     879     681     459     291     209
                 174     133
 
TatI w'GTAC_w

Cuts at:   8154    8154 
   Size:       8639
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3702    3734    8156    8188    3702 
   Size:         32    4422      32    4153

  Fragments arranged by size: 

                4422    4153      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    705    1497    5107    6436     705 
   Size:        792    3610    1329    2908

  Fragments arranged by size: 

                3610    2908    1329     792
 
XbaI T'CTAG_A

Cuts at:    600    4974    5236    5670     600 
   Size:       4374     262     434    3569

  Fragments arranged by size: 

                4374    3569     434     262
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1474    3616    4746    6134    1474 
   Size:       2142    1130    1388    3979

  Fragments arranged by size: 

                3979    2142    1388    1130
 
XhoI C'TCGA_G

Cuts at:    936    2172    5556     936 
   Size:       1236    3384    4019

  Fragments arranged by size: 

                4019    3384    1236
 
XmaI C'CCGG_G

Cuts at:   1193    1645    3040    5659    1193 
   Size:        452    1395    2619    4173

  Fragments arranged by size: 

                4173    2619    1395     452
 
XmnI GAAnn'nnTTC

Cuts at:   3194    3194 
   Size:       8639
 
ZraI GAC'GTC

Cuts at:   4354    4354 
   Size:       8639
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI     PsiI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AleI     AscI    AvrII     BaeI    BfrBI    BsiWI    BsrGI
   BssHII   BstEII     ClaI     FseI     HpaI     NdeI     NotI     NruI
     NsiI     PacI     PmlI    PpuMI    PshAI     PsrI    SacII    SanDI
    SexAI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
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