Restriction map for pF1KA0937
 (Circular) (Six-base) MAPSORT of: pF1KA0937.seq  Check: 8788  from: 1  to: 4970



pF1KA0937  4970 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    864     864 
   Size:       4970
 
AccI GT'mk_AC

Cuts at:   1436    2008    1436 
   Size:        572    4398
 
Acc65I G'GTAC_C

Cuts at:   1986    1986 
   Size:       4970
 
AclI AA'CG_TT

Cuts at:   4810    4810 
   Size:       4970
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    877    1759    2795    3227    3974    4242     877 
   Size:        882    1036     432     747     268    1605

  Fragments arranged by size: 

                1605    1036     882     747     432     268
 
AflIII A'CryG_T

Cuts at:   2322    3426    4403    2322 
   Size:       1104     977    2889

  Fragments arranged by size: 

                2889    1104     977
 
AgeI A'CCGG_T

Cuts at:   3358    3358 
   Size:       4970
 
AhdI GACnn_n'nnGTC

Cuts at:    288     288 
   Size:       4970
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    256     784     256 
   Size:        528    4442
 
AlwNI CAG_nnn'CTG

Cuts at:    811    1005    2275    3842     811 
   Size:        194    1270    1567    1939

  Fragments arranged by size: 

                1939    1567    1270     194
 
ApaI G_GGCC'C

Cuts at:    740    1381     740 
   Size:        641    4329
 
ApaLI G'TGCA_C

Cuts at:   3740    3740 
   Size:       4970
 
ApoI r'AATT_y

Cuts at:   1974    4323    4579    1974 
   Size:       2349     256    2365

  Fragments arranged by size: 

                2365    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4970
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4970
 
AvaI C'yCGr_G

Cuts at:   1022    1682    1990    1022 
   Size:        660     308    4002

  Fragments arranged by size: 

                4002     660     308
 
BamHI G'GATC_C

Cuts at:   1115    1995    1115 
   Size:        880    4090
 
BanI G'GyrC_C

Cuts at:    572     838    1313    1534    1786    1986    2400    2648    2683 
   Size:        266     475     221     252     200     414     248      35

Cuts at:   2683     572 
   Size:       2859

  Fragments arranged by size: 

                2859     475     414     266     252     248     221     200
                  35
 
BanII G_rGCy'C

Cuts at:    740     925    1381    1984    3014     740 
   Size:        185     456     603    1030    2696

  Fragments arranged by size: 

                2696    1030     603     456     185
 
BbeI G_GCGC'C

Cuts at:    576    2404    2652     576 
   Size:       1828     248    2894

  Fragments arranged by size: 

                2894    1828     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    865    1534     865 
   Size:        669    4301
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4936
 
BciVI GTATCCnnnnn_n'

Cuts at:   2868    3629    4970    2868 
   Size:        761    1341    2868

  Fragments arranged by size: 

                2868    1341     761
 
BclI T'GATC_A

Cuts at:   2490    2490 
   Size:       4970
 
BglII A'GATC_T

Cuts at:   2485    4522    2485 
   Size:       2037    2933
 
BlpI GC'TnA_GC

Cuts at:   2082    2082 
   Size:       4970
 
Bme1580I G_kGCm'C

Cuts at:    740    1381    2595    2688    3744     740 
   Size:        641    1214      93    1056    1966

  Fragments arranged by size: 

                1966    1214    1056     641      93
 
BmgBI CAC'GTC

Cuts at:   1434    1434 
   Size:       4970
 
BmrI ACTGGGnnnn_n'

Cuts at:    325     678     743     917    1011    1051    1121    2182    2263 
   Size:        353      65     174      94      40      70    1061      81

Cuts at:   2263    2598    4742     325 
   Size:        335    2144     553

  Fragments arranged by size: 

                2144    1061     553     353     335     174      94      81
                  70      65      40
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1872    3337    1872 
   Size:       1465    3505
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    800     879    1529    1776     800 
   Size:         79     650     247    3994

  Fragments arranged by size: 

                3994     650     247      79
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2146    3025    3517    3815    2146 
   Size:        879     492     298    3301

  Fragments arranged by size: 

                3301     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4674    4674 
   Size:       4970
 
BsaAI yAC'GTr

Cuts at:    507    2323     507 
   Size:       1816    3154
 
BsaBI GATnn'nnATC

Cuts at:      6    2484    4521       6 
   Size:       2478    2037     455

  Fragments arranged by size: 

                2478    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    165     415     573    2401    2649    3351    4847     165 
   Size:        250     158    1828     248     702    1496     288

  Fragments arranged by size: 

                1828    1496     702     288     250     248     158
 
BsaWI w'CCGG_w

Cuts at:    881     958    1610    1647    1710    2159    2680    3358    3632 
   Size:         77     652      37      63     449     521     678     274

Cuts at:   3632    3779    4190     881 
   Size:        147     411    1661

  Fragments arranged by size: 

                1661     678     652     521     449     411     274     147
                  77      63      37
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    340     370     391     421    1527    1557     340 
   Size:         30      21      30    1106      30    3753

  Fragments arranged by size: 

                3753    1106      30      30      30      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    348     348 
   Size:       4970
 
BseYI C'CCAG_C

Cuts at:    177     243     548    2395    3153    3730    4226     177 
   Size:         66     305    1847     758     577     496     921

  Fragments arranged by size: 

                1847     921     758     577     496     305      66
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1148    1317    1148 
   Size:        169    4801
 
BsiEI CG_ry'CG

Cuts at:    104     125     159    2512    2558    3766     104 
   Size:         21      34    2353      46    1208    1308

  Fragments arranged by size: 

                2353    1308    1208      46      34      21
 
BsiHKAI G_wGCw'C

Cuts at:    155    1984    2762    2952    3744     155 
   Size:       1829     778     190     792    1381

  Fragments arranged by size: 

                1829    1381     792     778     190
 
BsmI GAATG_Cn'

Cuts at:   1637    1637 
   Size:       4970
 
BsmBI CGTCTCn'nnnn_

Cuts at:    268     268 
   Size:       4970
 
Bsp1286I G_dGCh'C

Cuts at:    155     740     925    1381    1984    2595    2688    2762    2952 
   Size:        585     185     456     603     611      93      74     190

Cuts at:   2952    3014    3744     155 
   Size:         62     730    1381

  Fragments arranged by size: 

                1381     730     611     603     585     456     190     185
                  93      74      62
 
BspEI T'CCGG_A

Cuts at:    881    1610    1710    4190     881 
   Size:        729     100    2480    1661

  Fragments arranged by size: 

                2480    1661     729     100
 
BspHI T'CATG_A

Cuts at:   1121    4146    1121 
   Size:       3025    1945
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    293     864    1004    1513    2020    2234    2536    2917     293 
   Size:        571     140     509     507     214     302     381    2346

  Fragments arranged by size: 

                2346     571     509     507     381     302     214     140
            
 
BsrBI CCG'CTC

Cuts at:     50    3262    3316    4800    4965      50 
   Size:       3212      54    1484     165      55

  Fragments arranged by size: 

                3212    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1010    2882    1010 
   Size:       1872    3098
 
BsrFI r'CCGG_y

Cuts at:    214     569    2968    3149    3358     214 
   Size:        355    2399     181     209    1826

  Fragments arranged by size: 

                2399    1826     355     209     181
 
BsrGI T'GTAC_A

Cuts at:   1463    1463 
   Size:       4970
 
BssSI C'ACGA_G

Cuts at:   1750    3241    3599    1750 
   Size:       1491     358    3121

  Fragments arranged by size: 

                3121    1491     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2347    2347 
   Size:       4970
 
BstBI TT'CG_AA

Cuts at:   3331    3331 
   Size:       4970
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     825    1344    1896     114 
   Size:        711     519     552    3188

  Fragments arranged by size: 

                3188     711     552     519
 
BstYI r'GATC_y

Cuts at:      1    1115    1995    2462    2485    2820    3066    4078    4164 
   Size:       1114     880     467      23     335     246    1012      86

Cuts at:   4164    4176    4522       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1114    1012     880     467     449     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    614    1365     614 
   Size:        751    4219
 
BtgI C'CryG_G

Cuts at:    125     524     593     716     917    1872     125 
   Size:        399      69     123     201     955    3223

  Fragments arranged by size: 

                3223     955     399     201     123      69
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    854    2920    3101    3264     854 
   Size:       2066     181     163    2560

  Fragments arranged by size: 

                2560    2066     181     163
 
BtsI GCAGTG_nn'

Cuts at:    799    1501    1946    2213     799 
   Size:        702     445     267    3556

  Fragments arranged by size: 

                3556     702     445     267
 
DraI TTT'AAA

Cuts at:   1969    1969 
   Size:       4970
 
DrdI GACnn_nn'nnGTC

Cuts at:   2676    3534    2676 
   Size:        858    4112
 
EaeI y'GGCC_r

Cuts at:    122     156     628     974    1007    1428    1587    1869    2555 
   Size:         34     472     346      33     421     159     282     686

Cuts at:   2555    2729    3120    3147    4492     122 
   Size:        174     391      27    1345     600

  Fragments arranged by size: 

                1345     686     600     472     421     391     346     282
                 174     159      34      33      27
 
EagI C'GGCC_G

Cuts at:    122     156    2555     122 
   Size:         34    2399    2537

  Fragments arranged by size: 

                2537    2399      34
 
EarI CTCTTCn'nnn_

Cuts at:   1663    2993    3203    1663 
   Size:       1330     210    3430

  Fragments arranged by size: 

                3430    1330     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    295     316     556    3178    3488    3634    4780     295 
   Size:         21     240    2622     310     146    1146     485

  Fragments arranged by size: 

                2622    1146     485     310     240     146      21
 
EcoICRI GAG'CTC

Cuts at:   1982    1982 
   Size:       4970
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    800     877     879    1529    1759    1776    2795    3227    3974 
   Size:         77       2     650     230      17    1019     432     747

Cuts at:   3974    4242     800 
   Size:        268    1528

  Fragments arranged by size: 

                1528    1019     747     650     432     268     230      77
                  17       2
 
EcoO109I rG'GnC_Cy

Cuts at:    605     737     943    1044    1377    2109     605 
   Size:        132     206     101     333     732    3466

  Fragments arranged by size: 

                3466     732     333     206     132     101
 
EcoRI G'AATT_C

Cuts at:   1974    1974 
   Size:       4970
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2989    2989 
   Size:       4970
 
FspI TGC'GCA

Cuts at:   2751    2751 
   Size:       4970
 
HaeII r_GCGC'y

Cuts at:    555     576    1682    2404    2652    3674    4653     555 
   Size:         21    1106     722     248    1022     979     872

  Fragments arranged by size: 

                1106    1022     979     872     722     248      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    274     306     343     375     391     423     525     557    2812 
   Size:         32      37      32      16      32     102      32    2255

Cuts at:   2812    2842    2844    2874     274 
   Size:         30       2      30    2370

  Fragments arranged by size: 

                2370    2255     102      37      32      32      32      32
                  30      30      16       2
 
HincII GTy'rAC

Cuts at:   1089    2009    1089 
   Size:        920    4050
 
KasI G'GCGC_C

Cuts at:    572    2400    2648     572 
   Size:       1828     248    2894

  Fragments arranged by size: 

                2894    1828     248
 
KpnI G_GTAC'C

Cuts at:   1990    1990 
   Size:       4970
 
MluI A'CGCG_T

Cuts at:   4403    4403 
   Size:       4970
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1250    1525    3641    3825    1250 
   Size:        275    2116     184    2395

  Fragments arranged by size: 

                2395    2116     275     184
 
MscI TGG'CCA

Cuts at:    630     976    1009    1589    1871    2731     630 
   Size:        346      33     580     282     860    2869

  Fragments arranged by size: 

                2869     860     580     346     282      33
 
MslI CAynn'nnrTG

Cuts at:    430     455    1342    2959    3086     430 
   Size:         25     887    1617     127    2314

  Fragments arranged by size: 

                2314    1617     887     127      25
 
MspA1I CmG'CkG

Cuts at:    161     181     747    1132    2081    2395    2755    3768    4013 
   Size:         20     566     385     949     314     360    1013     245

Cuts at:   4013     161 
   Size:       1118

  Fragments arranged by size: 

                1118    1013     949     566     385     360     314     245
                  20
 
NaeI GCC'GGC

Cuts at:    216     571    3151     216 
   Size:        355    2580    2035

  Fragments arranged by size: 

                2580    2035     355
 
NarI GG'CG_CC

Cuts at:    573    2401    2649     573 
   Size:       1828     248    2894

  Fragments arranged by size: 

                2894    1828     248
 
NcoI C'CATG_G

Cuts at:    524     593     917    1872     524 
   Size:         69     324     955    3622

  Fragments arranged by size: 

                3622     955     324      69
 
NgoMIV G'CCGG_C

Cuts at:    214     569    3149     214 
   Size:        355    2580    2035

  Fragments arranged by size: 

                2580    2035     355
 
NspI r_CATG'y

Cuts at:    454     771     903    2023    2523    3430     454 
   Size:        317     132    1120     500     907    1994

  Fragments arranged by size: 

                1994    1120     907     500     317     132
 
PasI CC'CwG_GG

Cuts at:    367     640    1216    1926     367 
   Size:        273     576     710    3411

  Fragments arranged by size: 

                3411     710     576     273
 
PciI A'CATG_T

Cuts at:   3426    3426 
   Size:       4970
 
PflMI CCAn_nnn'nTGG

Cuts at:    811     811 
   Size:       4970
 
PfoI T'CCnGG_A

Cuts at:    409     777     409 
   Size:        368    4602
 
PmeI GTTT'AAAC

Cuts at:   1969    1969 
   Size:       4970
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4109    4141    4109 
   Size:         32    4938
 
PpuMI rG'GwC_Cy

Cuts at:    605     943    1044     605 
   Size:        338     101    4531

  Fragments arranged by size: 

                4531     338     101
 
PshAI GACnn'nnGTC

Cuts at:    260     260 
   Size:       4970
 
PspOMI G'GGCC_C

Cuts at:    736    1377     736 
   Size:        641    4329
 
PstI C_TGCA'G

Cuts at:    290    1001    2017    2702     290 
   Size:        711    1016     685    2558

  Fragments arranged by size: 

                2558    1016     711     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4970
 
PvuII CAG'CTG

Cuts at:    747    2395    2755     747 
   Size:       1648     360    2962

  Fragments arranged by size: 

                2962    1648     360
 
RsrII CG'GwC_CG

Cuts at:   3165    3165 
   Size:       4970
 
SacI G_AGCT'C

Cuts at:   1984    1984 
   Size:       4970
 
SalI G'TCGA_C

Cuts at:   2007    2007 
   Size:       4970
 
SanDI GG'GwC_CC

Cuts at:   1044    1044 
   Size:       4970
 
SapI GCTCTTCn'nnn_

Cuts at:   2993    3203    2993 
   Size:        210    4760
 
SbfI CC_TGCA'GG

Cuts at:   2017    2017 
   Size:       4970
 
ScaI AGT'ACT

Cuts at:   4487    4487 
   Size:       4970
 
SexAI A'CCwGG_T

Cuts at:   1403    1403 
   Size:       4970
 
SfcI C'TryA_G

Cuts at:     33     171     286     742     997    1300    1620    2013    2698 
   Size:        138     115     456     255     303     320     393     685

Cuts at:   2698    3691    3882      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1121     993     685     456     393     320     303     255
                 191     138     115
 
SfoI GGC'GCC

Cuts at:    574    2402    2650     574 
   Size:       1828     248    2894

  Fragments arranged by size: 

                2894    1828     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4970
 
SgrAI Cr'CCGG_yG

Cuts at:    569     569 
   Size:       4970
 
SmaI CCC'GGG

Cuts at:   1024    1992    1024 
   Size:        968    4002
 
SmlI C'TyrA_G

Cuts at:   2125    3040    3532    3794    2125 
   Size:        915     492     262    3301

  Fragments arranged by size: 

                3301     915     492     262
 
SnaBI TAC'GTA

Cuts at:    507     507 
   Size:       4970
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4970
 
SphI G_CATG'C

Cuts at:    454     903    2023    2523     454 
   Size:        449    1120     500    2901

  Fragments arranged by size: 

                2901    1120     500     449
 
StyI C'CwwG_G

Cuts at:    524     593     917    1097    1872    2104    2458     524 
   Size:         69     324     180     775     232     354    3036

  Fragments arranged by size: 

                3036     775     354     324     232     180      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1193    1331    1552    1607    2553    2686    3145    3354    1193 
   Size:        138     221      55     946     133     459     209    2809

  Fragments arranged by size: 

                2809     946     459     221     209     138     133      55
            
 
TatI w'GTAC_w

Cuts at:   1463    4485    1463 
   Size:       3022    1948
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1527    1559    4487    4519    1527 
   Size:         32    2928      32    1978

  Fragments arranged by size: 

                2928    1978      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1816    2767    1816 
   Size:        951    4019
 
XbaI T'CTAG_A

Cuts at:   2001    2001 
   Size:       4970
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1582    2465    1582 
   Size:        883    4087
 
XmaI C'CCGG_G

Cuts at:   1022    1990    1022 
   Size:        968    4002
 
XmnI GAAnn'nnTTC

Cuts at:    301    1672     301 
   Size:       1371    3599
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AleI     AloI     AscI    AvrII     BaeI
    BbvCI    BfrBI     BglI     BmtI   Bpu10I    BsiWI   BssHII   BstEII
  BstZ17I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PmlI     PsiI    PspXI     PsrI    SacII     SfiI     SrfI
     SspI     StuI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com