Restriction map for pF1KA0850
 (Linear) (Six-base) MAPSORT of: KIAA0850.seq  Check: 6711  from: 1  to: 1926



KIAA0850.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1108    1926 
   Size:       1108     818
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     355     515     863    1926 
   Size:         91     264     160     348    1063

  Fragments arranged by size: 

                1063     348     264     160      91
 
AflII C'TTAA_G

Cuts at:      0     511    1926 
   Size:        511    1415
 
AhdI GACnn_n'nnGTC

Cuts at:      0     109    1926 
   Size:        109    1817
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1649    1681    1926 
   Size:       1649      32     245

  Fragments arranged by size: 

                1649     245      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     244    1926 
   Size:        244    1682
 
ApoI r'AATT_y

Cuts at:      0      36     206     220     414     488    1852    1926 
   Size:         36     170      14     194      74    1364      74

  Fragments arranged by size: 

                1364     194     170      74      74      36      14
 
BanII G_rGCy'C

Cuts at:      0     976    1218    1903    1926 
   Size:        976     242     685      23

  Fragments arranged by size: 

                 976     685     242      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      41    1135    1926 
   Size:         41    1094     791

  Fragments arranged by size: 

                1094     791      41
 
BbvCI CC'TCA_GC

Cuts at:      0     722    1926 
   Size:        722    1204
 
BclI T'GATC_A

Cuts at:      0     681    1926 
   Size:        681    1245
 
Bme1580I G_kGCm'C

Cuts at:      0    1488    1926 
   Size:       1488     438
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     609    1926 
   Size:        609    1317
 
BmtI G_CTAG'C

Cuts at:      0     151    1926 
   Size:        151    1775
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1676    1926 
   Size:       1676     250
 
Bpu10I CC'TnA_GC

Cuts at:      0     722     854    1926 
   Size:        722     132    1072

  Fragments arranged by size: 

                1072     722     132
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1903    1926 
   Size:       1903      23
 
BsaAI yAC'GTr

Cuts at:      0     778    1926 
   Size:        778    1148
 
BsaBI GATnn'nnATC

Cuts at:      0     194     233    1926 
   Size:        194      39    1693

  Fragments arranged by size: 

                1693     194      39
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     466     496     972    1002    1643    1649    1673    1679 
   Size:        466      30     476      30     641       6      24       6

Cuts at:   1679    1926 
   Size:        247

  Fragments arranged by size: 

                 641     476     466     247      30      30      24       6
                   6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     514     517    1664    1926 
   Size:        514       3    1147     262

  Fragments arranged by size: 

                1147     514     262       3
 
BseYI C'CCAG_C

Cuts at:      0     565    1795    1926 
   Size:        565    1230     131

  Fragments arranged by size: 

                1230     565     131
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     781    1577    1926 
   Size:        625     156     796     349

  Fragments arranged by size: 

                 796     625     349     156
 
BsiHKAI G_wGCw'C

Cuts at:      0     976    1380    1926 
   Size:        976     404     546

  Fragments arranged by size: 

                 976     546     404
 
BsmI GAATG_Cn'

Cuts at:      0    1162    1926 
   Size:       1162     764
 
Bsp1286I G_dGCh'C

Cuts at:      0     976    1218    1380    1488    1903    1926 
   Size:        976     242     162     108     415      23

  Fragments arranged by size: 

                 976     415     242     162     108      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     345    1108    1926 
   Size:        345     763     818

  Fragments arranged by size: 

                 818     763     345
 
BsrDI GCAATG_nn'

Cuts at:      0     576    1797    1854    1926 
   Size:        576    1221      57      72

  Fragments arranged by size: 

                1221     576      72      57
 
BsrGI T'GTAC_A

Cuts at:      0    1541    1926 
   Size:       1541     385
 
BstBI TT'CG_AA

Cuts at:      0    1146    1926 
   Size:       1146     780
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1638    1926 
   Size:       1638     288
 
Bsu36I CC'TnA_GG

Cuts at:      0      73    1926 
   Size:         73    1853
 
BtsI GCAGTG_nn'

Cuts at:      0     149     272     741    1835    1926 
   Size:        149     123     469    1094      91

  Fragments arranged by size: 

                1094     469     149     123      91
 
DraIII CAC_nnn'GTG

Cuts at:      0     778    1926 
   Size:        778    1148
 
EaeI y'GGCC_r

Cuts at:      0      84     117     786    1272    1926 
   Size:         84      33     669     486     654

  Fragments arranged by size: 

                 669     654     486      84      33
 
EarI CTCTTCn'nnn_

Cuts at:      0     491     635     647     831    1926 
   Size:        491     144      12     184    1095

  Fragments arranged by size: 

                1095     491     184     144      12
 
EcoICRI GAG'CTC

Cuts at:      0     974    1926 
   Size:        974     952
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     355     515     863    1676    1926 
   Size:         91     264     160     348     813     250

  Fragments arranged by size: 

                 813     348     264     250     160      91
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      95     127    1168    1200    1926 
   Size:         95      32    1041      32     726

  Fragments arranged by size: 

                1041     726      95      32      32
 
HindIII A'AGCT_T

Cuts at:      0     514    1011    1926 
   Size:        514     497     915

  Fragments arranged by size: 

                 915     514     497
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     512    1765    1926 
   Size:        512    1253     161

  Fragments arranged by size: 

                1253     512     161
 
MscI TGG'CCA

Cuts at:      0      86     119     788    1274    1926 
   Size:         86      33     669     486     652

  Fragments arranged by size: 

                 669     652     486      86      33
 
MslI CAynn'nnrTG

Cuts at:      0    1280    1636    1926 
   Size:       1280     356     290

  Fragments arranged by size: 

                1280     356     290
 
MspA1I CmG'CkG

Cuts at:      0     398     679    1926 
   Size:        398     281    1247

  Fragments arranged by size: 

                1247     398     281
 
NdeI CA'TA_TG

Cuts at:      0    1419    1926 
   Size:       1419     507
 
NheI G'CTAG_C

Cuts at:      0     147    1926 
   Size:        147    1779
 
PmlI CAC'GTG

Cuts at:      0     778    1926 
   Size:        778    1148
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1649    1681    1926 
   Size:       1649      32     245

  Fragments arranged by size: 

                1649     245      32
 
PshAI GACnn'nnGTC

Cuts at:      0    1509    1926 
   Size:       1509     417
 
PstI C_TGCA'G

Cuts at:      0     160     324    1119    1518    1926 
   Size:        160     164     795     399     408

  Fragments arranged by size: 

                 795     408     399     164     160
 
PvuII CAG'CTG

Cuts at:      0     398     679    1926 
   Size:        398     281    1247

  Fragments arranged by size: 

                1247     398     281
 
SacI G_AGCT'C

Cuts at:      0     976    1926 
   Size:        976     950
 
SapI GCTCTTCn'nnn_

Cuts at:      0     635    1926 
   Size:        635    1291
 
ScaI AGT'ACT

Cuts at:      0     987    1926 
   Size:        987     939
 
SfcI C'TryA_G

Cuts at:      0     156     320     839    1115    1514    1808    1926 
   Size:        156     164     519     276     399     294     118

  Fragments arranged by size: 

                 519     399     294     276     164     156     118
 
SmlI C'TyrA_G

Cuts at:      0     511    1882    1926 
   Size:        511    1371      44

  Fragments arranged by size: 

                1371     511      44
 
StyI C'CwwG_G

Cuts at:      0     520    1783    1926 
   Size:        520    1263     143

  Fragments arranged by size: 

                1263     520     143
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     591    1644    1926 
   Size:        591    1053     282

  Fragments arranged by size: 

                1053     591     282
 
TatI w'GTAC_w

Cuts at:      0     668     843     985    1233    1541    1926 
   Size:        668     175     142     248     308     385

  Fragments arranged by size: 

                 668     385     308     248     175     142
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1467    1499    1643    1675    1926 
   Size:       1467      32     144      32     251

  Fragments arranged by size: 

                1467     251     144      32      32
 
XbaI T'CTAG_A

Cuts at:      0     379    1926 
   Size:        379    1547
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1722    1827    1926 
   Size:       1722     105      99

  Fragments arranged by size: 

                1722     105      99
 
XmnI GAAnn'nnTTC

Cuts at:      0     656    1142    1926 
   Size:        656     486     784

  Fragments arranged by size: 

                 784     656     486
 

 Enzymes that do cut:

     AarI     AcuI    AflII     AhdI     AloI    AlwNI     ApoI    BanII
     BbsI    BbvCI     BclI Bme1580I     BmrI     BmtI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspMI    BsrDI    BsrGI    BstBI    BstXI   Bsu36I
     BtsI   DraIII     EaeI     EarI  EcoICRI  Eco57MI    Hin4I  HindIII
     MmeI     MscI     MslI   MspA1I     NdeI     NheI     PmlI     PpiI
    PshAI     PstI    PvuII     SacI     SapI     ScaI     SfcI     SmlI
     StyI    TaqII     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AleI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI    BmgBI     BplI     BsaI    BsaHI    BsaWI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI   BstEII    BstYI  BstZ17I     BtgI    BtgZI     ClaI    CspCI
     DraI     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XhoI     XmaI     ZraI
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